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Phytochemical Assay and Antiplatelet Activity of Fractions of Velvet Bean Seeds (Mucuna pruriens L.) WIDOWATI, WAHYU; RATNAWATI, HANA; RUSDI, UDJU DJUNAEDI; WINARNO, WAHYU; IMMANUEL, VICTOR
HAYATI Journal of Biosciences Vol 17, No 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.207 KB) | DOI: 10.4308/hjb.17.2.85

Abstract

Platelet aggregation is an important factor contributing to the formation of thrombus due to an uncontrolled blood clotting. An antiplatelet agent is a compound which decreases platelet aggregation and inhibits thrombus formation. The objectives of this study were to determine the class of compound employing phytochemical assay and to determine the in vitro antiplatelet activity of four fraction, namely hexane, ethyl acetate, butanol, and water fractions of velvet bean seeds (Mucuna pruriens L.) using epinephrine (EPN) as agonist of platelet aggregation. The antiplatelet activities were tested in human platelet rich plasma with hyperaggregation. To determine the activities, EPN was arranged at 4 level of concentrations  (300, 150, 75, and 30 μM), and antiplatelet agents were at 500 µg/ml. The results indicated that ethyl acetate, butanol and water fraction contained high flavonoids and moderate phenols. The water, butanol and ethyl acetate fractions of velvet bean seeds exhibited potential inhibition of EPN-induced platelet aggregation at all concentrations. The strongest antiplatelet agent was water fraction and had the same antiplatelet activity as aspirin at level 150, 75, and 30 μM of EPN. Butanol fraction had the same antiplatelet activity as aspirin at the lowest EPN (30 μM).
PENGARUH PELATIHAN KERJA, DISIPLIN KERJA DAN MOTIVASI TERHADAP KINERJA PADA PT. DUA MITRA LANGGENG SURAKARTA Winarno, Wahyu
KELOLA Vol 5, No 2 (2018): December
Publisher : KELOLA

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Abstract

The purpose of this study is to determine the effect of job training, work discipline and motivation on employee performance PT. Dua Mitra Langgeng Surakarta. Population in this research all employees of PT. Two partners Langgeng numbered 250 people, while the sample used as many as 100 people who become respondents and sampling techniques of this study using accidental sampling technique. Based on the results analysis known to job training, work discipline and motivation have an effect on signifikan to employee performance of PT. Dua Mitra Langgeng Surakarta. The simultaneous test results of job training variables, work discipline and motivation have a significant effect on employee performance variable of PT. Dua Mitra Langgeng Surakarta.Keywords: Job Training, Work Discipline, Motivation and Performance
PEMANFAATAN BEBERAPA PERANGKAP WARNA BERPEREKAT DALAM MENGENDALIKAN HAMA PADA TANAMAN KEDELAI VARIETAS WILIS Erdiansyah, Iqbal; Winarno, Wahyu; Pambudi, Nanang Setyo
AGRITROP Vol 17, No 1 (2019): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v17i1.2186

Abstract

Penelitian bertujuan untuk mengetahui keefektifan pengendalian hama menggunakan perangkap warna berperekat terhadap tanaman kedelai varietas wilis Penelitian ini dilaksanakan pada bulan Agustus 2017 sampai dengan bulan Oktober 2017. Bertempat di Desa Ampel Krajan Kecamatan Wuluhan Kabupaten Jember. Penelitian ini dilakukan dengan menggunakan Rancangan Acak Kelompok (RAK) Non Faktorial yaitu pemberian perekat berwarna yang terdiri dari 5 level yang diulang sebanyak 6 kali yaitu warna kuning, warna hijau, warna putih, warna merah, dan warna biru. Pengamatan dilakukan terhadap parameter macam-macam serangga, jumlah hama dan intensitas serangan hama. Hasil penelitian menunjukan bahwa Pemanfaatan beberapa perangkap pada tanaman kedelai varietas Willis memberikan pengaruh nyata (significant) terhadap parameter jumlah hama yang terperangkap. Perangkap warna kuning merupakan perangkap warna yang efektif karena hama lebih tertarik pada warna kuning.
The Antiplatelet Aggregation Effect of Extract And Ethyl Acetate Fraction of Velvet Bean Seed (Mucuna pruriens L.) in Dyslipidemic Rat Widowati, Wahyu; Ratnawati, Hana; Rusdi, Udju Djunaedi; Winarno, Wahyu; Kasim, Felix
Agritech Vol 31, No 1 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9726

Abstract

Cardiovascular disease (CVD) is the first cause of death in the world, CVD has complex and multifactorial process including atherogenic lipoprotein, oxidized low density lipoprotein (LDL), endothelial dysfunction, plaque stability, vascular inflammation, thrombotic and fibrinolytic disorder, exercises and genetic factor. Inhibiting the platelet ag- gregation is one of the CVD prevention. Velvet bean seed (Mucuna pruriens L.) can be found abundantly in Indonesia, but has not been used as herbal medicine. Ethanol extract and ethyl acetate of velvet bean seed contain high flavonoids and antioxidants properties which is expected could inhibit platelet aggregation. The objectives of the research were to determine the activity of ethanol extract and ethyl acetate fraction of velvet bean seed towards clotting and bleeding time in dyslipidemic rats. This research used completely randomized design in dyslipidemic rats which were given by ethanol extract of velvet bean seed at the concentrations of 50, 100 and 200 mg/kg BW/day and ethyl acetate fraction of velvet bean seed at the concentrations of 15, 30 and 60 mg/kg BW/day and 42.2 mg/kg BW/day aspirin for ten days. Clotting and bleeding time were measured at days 0, 10, and 20. Data were analyzed using One way analysis of vari- ance and continued with Duncan’s post Hoc test with 95 % level of significancy. The results showed that administration of 60 mg/kg BW/day ethyl acetate fraction of velvet bean seed and at the concentrations of 100 and 200 mg/kg BW/ day ethanol extract of velvet bean seed, prolong the clotting time at day 10, ethyl acetate fraction at the concentration of 60 mg/kg BW/day, 200 mg/kg BW/day ethanol extract of velvet bean prolong bleeding time at day 10.
Phytochemical Assay and Antiplatelet Activity of Fractions of Velvet Bean Seeds (Mucuna pruriens L.) WIDOWATI, WAHYU; RATNAWATI, HANA; RUSDI, UDJU DJUNAEDI; WINARNO, WAHYU; IMMANUEL, VICTOR
HAYATI Journal of Biosciences Vol 17, No 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.17.2.85

Abstract

Platelet aggregation is an important factor contributing to the formation of thrombus due to an uncontrolled blood clotting. An antiplatelet agent is a compound which decreases platelet aggregation and inhibits thrombus formation. The objectives of this study were to determine the class of compound employing phytochemical assay and to determine the in vitro antiplatelet activity of four fraction, namely hexane, ethyl acetate, butanol, and water fractions of velvet bean seeds (Mucuna pruriens L.) using epinephrine (EPN) as agonist of platelet aggregation. The antiplatelet activities were tested in human platelet rich plasma with hyperaggregation. To determine the activities, EPN was arranged at 4 level of concentrations  (300, 150, 75, and 30 ?M), and antiplatelet agents were at 500 µg/ml. The results indicated that ethyl acetate, butanol and water fraction contained high flavonoids and moderate phenols. The water, butanol and ethyl acetate fractions of velvet bean seeds exhibited potential inhibition of EPN-induced platelet aggregation at all concentrations. The strongest antiplatelet agent was water fraction and had the same antiplatelet activity as aspirin at level 150, 75, and 30 ?M of EPN. Butanol fraction had the same antiplatelet activity as aspirin at the lowest EPN (30 ?M).
Free Radical Scavenging and α-Glucosidase Inhibitor Activity of Ethanolic Extract of Mucuna pruriens L. Widowati, Wahyu; Ratnawati, Hana; Retnaningsih, Ch.; Lindayani, .; Rusdi, Udju D.; Winarno, Wahyu
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 5, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Biji kacang “koro benguk” atau velvet bean sudah sangat dikenal tetapi dasar ilmiah penggunaannya sebagai obat tradisional terutama sebagai antioksidan dan antidiabetik masih belum diketahui. Oleh sebab itu dalam penelitian ini dilakukan percobaan untuk mengevaluasi aktivitas antioksidan dan antidiabetik dari ekstrak biji koro benguk, serta fraksi heksana, etil asetat, butanol dan air menggunakan uji in vitro menggunakan DPPH (1,1-difenil-2-pikril-hidrazil) dengan pembanding positif kuersetin dan BHA. Hasil penelitian menunjukkan bahwa IC50 DPPH untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol dan air berturut-turut sebesar 22,17; 152,60; 113,03; 4,93; dan 9,14 μg/mL, sedangkan untuk kuersetin dan BHA masing masing sebesar  4,28 dan 22,587 μg/mL. Aktivitas antidiabetik ditentukan menggunakan metode penghambatan α-glukosidase dengan glucobay sebagai kontrol positif. Hasil penelitian menunjukkan bahwa IC50 antidiabetik untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol, air, dan glucobay berturut-turut sebesar 24,44; 19,55; 8,40; 80,98; 48.97; dan 16,23 μg/mL. ABSTRACT Velvet bean seeds are ubiquitous but the scientific basis for its medicinal use especially as antioxidant and antidiabetic remains unknown. Therefore, a study was designed to evaluate  antioxidant and antidiabetic activities of the  extract, hexane, ethyl acetate, butanol and water fractions of velvet bean seeds. In order to evaluate antioxidant activity of extract and fractions  using  quercetin,  butylated hydroxyanisole (BHA) as positive control and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity was determined. The results showed that IC50 of DPPH were 22.17; 152.60; 113.03; 4.93; 9.14 μg/mL respectevily;  quercetin 4.28 and BHA 22.587 μg/mL.  Antidiabetic activity was determined by using  of α-glucosidase inhibition assay of  extract, four fractions and glucobay as positive control. The results showed that antidiabetic IC50 were24.44; 19.55; 8.40; 80.98; 48.97μg/mL respectively and glucobay as positive control  16.23 μg/mL.
Respon Pertumbuhan dan Hasil Tanaman Kentang (Solanum tuberosum L.) Terhadap Pemberian Naungan dan Pupuk Kieserite di Dataran Medium Purnomo, Dedi; Damanhuri, FNU; Winarno, Wahyu
Agriprima, Journal of Applied Agricultural Sciences Vol 2, No 1 (2018): MARET
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.136 KB) | DOI: 10.25047/agriprima.v2i1.72

Abstract

Pengembangan budidaya kentang perlu dilakukan untuk meningkatkan hasil produksi nasional, salah satu langkahnya yaitu dengan mengembangkan kentang di dataran medium 300 sampai 700 m dpl. Penelitian ini bertujuan untuk mengembangkan teknologi budidaya kentang pada dataran medium melalui pengaplikasian pupuk kieserite (Mg) dan naungan. Pelaksanaan penelitian di mulai dari bulan Agustus 2016 sampai dengan bulan Desember 2016. Penelitian ini menggunakan rancangan acak kelompok (RAK) faktorial yang terdiri dari dua faktor. Faktor pertama yaitu pupuk kieserite terdiri dari lima taraf : 0 kg/ha, 50 kg/ha, 100 kg/ha, 150 kg/ha, dan 200 kg/ha. Faktor kedua yaitu naungan dengan kerapatan 60% dan tanpa naungan. Hasil analisis data menunjukkan penggunaan naungan dengan kerapatan 60% berpengaruh sangat nyata pada tinggi tanaman umur 14 HST, 28 HST, dan 42 HST, luas daun 42 HST, serta berpengaruh sangat nyata terhadap jumlah umbi persampel, berat umbi persampel dan tidak berpengaruh nyata pada jumlah cabang tanaman. Perlakuan pupuk kieserite (Mg) memberikan pengaruh nyata pada jumlah cabang tanaman umur 42 HST, tetapi tidak berpengaruh terhadap tinggi tanaman umur 14 HST, 28 HST, dan 42 HST, luas daun 42 HST, jumlah umbi persampel, berat umbi persampel. Interaksi antara naungan dan pupuk kieserite (Mg) tidak  memberikan pengaruh nyata pada tinggi tanaman, luas daun, jumlah cabang, jumlah umbi, dan berat umbi, hal ini diduga karena kurang sesuainya dosis dan intensitas pemberian pupuk kieserite (Mg) yang di aplikasikan.
Pemanfaatan Ektrak Daun Pepaya (Carica papaya L.) Sebagai Insektisida Nabati Pengendali Walang Sangit (Leptocorisa acuta) Pada Tanaman Padi Listianti, Nafa Novika; Winarno, Wahyu; Erdiansyah, Iqbal
Agriprima, Journal of Applied Agricultural Sciences Vol 3, No 1 (2019): MARET
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.292 KB) | DOI: 10.25047/agriprima.v3i1.142

Abstract

Keberadaan hama walang sangit (L. acuta) sebagai hama utama tanaman padi menyebabkan produksi padi rendah. Pengendalian walang sangit yang biasanya menggunakan insektisida sintetik dinilai kurang efektif karena hama telah resisten terhadap insektisida sintetik. Upaya pengendalian walang sangit (L. acuta) dapat dilakukan dengan memanfaatkan bahan yang berasal dari organ tanaman. Penelitian ini bertujuan untuk menekan aktivitas serangan hama walang sangit (L. acuta). Jalan Parangtritis, Kelurahan Antirogo, Kecamatan Sumbersari, Jember merupakan tempat pelaksanaan penelitian. Penelitian ini disusun dengan RAK (Rancangan Acak Kelompok) Non Faktorial, menggunakan tiga perlakuan dan diulang sembilan kali. Perlakuan meliputi P0 (insektisida sintetik bahan aktif deltametrin 1 ml/liter), P1 (konsentrasi ekstrak daun pepaya 55%), dan P2 (konsentrasi ekstrak daun pepaya 75 %). Hasil penelitian menunjukkan bahwa perlakuan P2 (konsentrasi 75 % ekstrak daun pepaya) mampu menekan intensitas serangan walang sangit (L. acuta) pada pengamatan minggu ke 3 dan ke 4 dengan rata-rata intensitas serangan 26,31 % sampai 23,17 %. Selain itu, pada perlakuan P2 (75 % ekstrak daun pepaya) berpengaruh pada persentase bulir bernas per sampel dengan rerata 38,09 % dan berat padi per sampel dengan rerata 7,11 gram.