Articles

Kualitas Agarosa Hasil Isolasi dari Rhodymenia ciliata Menggunakan Deae-Selulosa Salamah, Ella; Susanti, Dyah; Wikanta, Thamrin
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 8, No 1 (2005): Buletin Teknologi Hasil Perikanan
Publisher : Departement of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (37.868 KB)

Abstract

Penelitian ini bertujuan untuk menguji pengaruh penambahan DEAE-selulosa terhadap kualitas agarosa dari bahan baku Rhodymenia ciliata. Penelitian terdiri dari dua tahap yaitu penelitian pendahuluan yang bertujuan untuk menentukan konsentrasi NaOH optimum pada pra-ekstraksi agar dan penelitian utama yang bertujuan untuk menguji pengaruh perbandingan DEAE-selulosa dengan rumput laut Rhodymenia ciliata terhadap kualitas agarosa. Hasil penelitian menunjukkan bahwa pada perlakuan pemberian DEAE-selulosa 27% terhadap berat rumput laut kering menghasilkan agarosa optimum dengan sifat-sifat fisik yang terbaik.Kata Kunci : Agarosa, DEAE-selulosa
EXTRACTION OF POLYPHENOL FROM Sargassum sp. AND ITS ENTRAPMENT IN THE NANOCHITOSAN Wikanta, Thamrin
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Seaweed is substantial in bioactive compounds of polyphenols that can scavengefree radicals. The aim of this study was to determine the total content of polyphenoliccompounds in the Sargassum sp and its aktivities by using DPPH and FRAP methods.DPPH methods resulting the value of IC50 (Inhibition concentration) and FRAPmethods resulting the total of Fe3+ reduced to Fe2+. Entrapment of Sargassum sp. extractusing nanoparticle chitosan-based was to protect the active compounds in the extract tokeep well the activity of the polyphenol of extraction. Results showed that totalpolyphenol contents of Sargassum polycistum and S. hystrix at 10 mg/ml were 108,64μg/mL and 65,5 μg/mL respectively. IC50 value on DPPH test of S. polycistum and S.hystrix were 667,234 μg/mL and 2861,49 μg/mL, respectively. The reasults of FRAPtest of S. polycistum extracts at concentrations of 100, 500, and 1000 μg/mL were 67,00;127,14; and 214,59 μmol/μg, respectively, while the S. hystrix extract was 48,47; 62,48;80,22μmol/μg extract. Loading capacity of chitosan-STPP and chitosan-carrageenan onpolyphenol were 0,45 and 0,34%, respectively, and entrapment efficiency of Chitosan-STPP and Chitosan-carrageenan were 21,96% and 39,62%, respectively.Keywords: Polyphenol, Sargassum sp, antioxidant , and nanochitosan
Kualitas Agarosa Hasil Isolasi dari Rhodymenia ciliata Menggunakan Deae-Selulosa Salamah, Ella; Susanti, Dyah; Wikanta, Thamrin
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 8, No 1 (2005): Buletin Teknologi Hasil Perikanan
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (37.868 KB) | DOI: 10.17844/jphpi.v8i1.1026

Abstract

Penelitian ini bertujuan untuk menguji pengaruh penambahan DEAE-selulosa terhadap kualitas agarosa dari bahan baku Rhodymenia ciliata. Penelitian terdiri dari dua tahap yaitu penelitian pendahuluan yang bertujuan untuk menentukan konsentrasi NaOH optimum pada pra-ekstraksi agar dan penelitian utama yang bertujuan untuk menguji pengaruh perbandingan DEAE-selulosa dengan rumput laut Rhodymenia ciliata terhadap kualitas agarosa. Hasil penelitian menunjukkan bahwa pada perlakuan pemberian DEAE-selulosa 27% terhadap berat rumput laut kering menghasilkan agarosa optimum dengan sifat-sifat fisik yang terbaik.Kata Kunci : Agarosa, DEAE-selulosa
Ekstraksi Khitosan dari Kepala Udang Putih (Penaeus merguensis) Yunizal, Yunizal; Indriati, Ninoek; Murdinah, Murdinah; Wikanta, Thamrin
Agritech Vol 21, No 3 (2001)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.13597

Abstract

In order to optimize the utilization of shrimp head, study on extraction of chitosan from head of white shrimp (Panaeud merguensis) was carried out. The aim of the study was to find out the optimum conditions of deproteinization, demineralization and deacetylation process in the extraction of chitosan. The result showed that the most suitable condition of deproteinization process was obained by boiling shrimp head in solution of NaOH 3 % at 100°C for 90 minutes with the ratio of solution and shrimp head 6 : 1 (v/w). However the most suitable condition of demineralization process was obtained by dipping of deproteinized shrimp head in HCl 1,25N solution for 20 hours at ambient temperature. Futhermore, the most suitable condition for deacetylation process was achieved by heating demineralized shrimp head in NaOH 50% solution at 100°C for 90 minutes. As final product, chitosan contained: 6.73% moisture, 6.74% total nitrogen (d.b), 1.00% totla ash (d.b), while the degree of deacetylation and viscosity were 83.10% and 3.50 cps, respectively.
SYNTHESIS OF POLYVINYL ALCOHOL-CHITOSAN HYDROGEL AND STUDY OF ITS SWELLING AND ANTIBACTERIAL PROPERTIES Wikanta, Thamrin; Erizal, Mr; Tjahyono, Mr; Sugiyono, Mr
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 1 (2012): May 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.071 KB) | DOI: 10.15578/squalen.v7i1.10

Abstract

The aim of this research was to synthesize a hydrogel for wound dressing by mixing of polyvinyl alcohol (PVA) and chitosan (CTS) and processed by combination technique of freezing-thawing and irradiation by gamma ray, and to study of its properties. PVA aqueous solution 10% (w/v) was mixed with 2% (w/v) chitosan (CTS) solution and homogenized. The PVA-CTS mixture was processed by freezing-thawing up to 3 cycles, and then irradiated by gamma rays at the doseranged of 20-50 kGy  (dose rate was 10 kGy/hour). Result showed that PVA-CTS hydrogel with the gel fraction of 83%, 87%, 90%, and 83% were obtained at the irradiation dose of 20 kGy, 30 kGy, 40 kGy, and 50 kGy, respectively. Increasing of irradiation dose caused increasing of water absorption of hydrogel, i.e. 1.700 %, 1.715 %, 1.913 %, and 2.036 %, respectively, and the hydrogel reached the equilibrium in 25 hours. The hydrogel showed very slow water evaporation rate (~ 2%) at the initial time (1 hour) and then increased very fast (up ~50 %) at 24 h, i.e. 43%, 39.13%, 44%, and 53%, respectively. The elongation at break of hydrogels were obtained 245%, 322%, 322%, and 205% with the maximum value were obtained at irradiation dose ranged of 30-40 kGy. The presence of chitosan in the PVA hydrogel made it having higher antibacterial properties with the inhibitionzone value of 8 mm at irradiation dose of 30-40 kGy compared to PVA hydrogel as a negative control (6 mm) and to chloramphenicol as a positive control (8 mm).
Correlation between fucoxanthin contents in Turbinaria sp.and sea water nutrients at Binuangeun and Krakal Coasts Januar, Hedi Indra; Wikanta, Thamrin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 1 (2011): May 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.043 KB) | DOI: 10.15578/squalen.v6i1.57

Abstract

Fucoxanthin pigment is a potential compound in nutraceutical. It is commonly found in algaesuch as in Turbinaria sp.,a very abundant species in Indonesian coastal. To maximize theproduction of fucoxanthin for the development of nutraceutical industry in the coastal area, it isimportant to identify the optimal environmental parameters for its biosynthesis. It has been reportedthat nitrogen and or phosphate nutrients affect the growth of algae. Therefore, this paper aimed toobtain an optimal environment for Turbinaria sp. to produce fucoxanthin based on correlationanalysis. Correlated data were fucoxanthin isolated from Turbinaria sp.that was collected fromKrakal (Yogyakarta) and Binuangeun (Banten) coasts. Fucoxanthin contents in each sample werecompared based on the peak areas in chromatogram after 1 mg injection of n-hexane extract ofT. decurrrens in the HPLC system. The analysis results showed that the peak areas of fucoxanthinwere significantly different among sampling locations (P = 7.99 x 10-6), sampling periods (P = 6 x10-3) and interaction of both (P = 3 x 10-3). Whereas the correlation analysis revealed that interactionbetween phosphate and temperature could be a predictor that strongly (R = 0.986) and significantlycorrelated (P = 1.09 x 10-7) with the peak areas of fucoxanthin (R2= 96.5%). These results were inline with growth limiting factor in seawater and the function of fucoxanthin as ultraviolet protector.Based on these results, it is suggested that the suitable location for fucoxanthin production is in thearea of agricultural run-off that contains an elevated concentration of dissolved phosphate.
Chitosan Composite of Crab Shell and Hydroxyapatite of Tuna Fish Bone as Biomaterials for Guided Tissue Regeneration Wikanta, Thamrin; Erizal, Erizal; Sugiyono, Sugiyono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 3 (2013): December 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.257 KB) | DOI: 10.15578/squalen.v8i3.85

Abstract

The needs of membrane biomaterials to prevent the migration process of epithelial cells into the bony area after tooth withdrawal process, for filler the holed tooth and to cure gingivitis have increased significantly. In this study, a biomembranes composed of chitosan (CTS) and hydroxyapatite (HA) were prepared. CTS was made of crab shell and HA was made of tuna fish bone. There were 4 groups of treatments, i.e.: K0: only 4 g CTS as a control; K1: combination of 4 g CTS and 2 g HA; K2: combination of 4 g CTS and 4 g HA; and K3: combination of 4 g CTS and 6 g HA. The dried and solid membrane was soaked in 5% Na2SO3 for 1 hour, and then dried in oven at 60 0C for 24 hours. The composite membrane properties, i.e.: water absorption, biodegradation, tensile strength, elongation at break, functional group (by FTIR), and microstructure (by SEM) were evaluated. Result showed that the increasing of HA content in membrane will decreasing the water absorption. The increasing of HA content in membrane and soaking time in Phosphate Buffered Saline (PBS), can increase in biodegradation rate. The biodegradation rate of membrane was about 4.44-6.01% during 27 days of soaking time. The increasing soaking time in PBS can decrease the tensile strength of membrane at about 18.33%-21.17% and decreased the elongation at break at about 26.64%-59.94%. FT-IR analysis indicated that there was a crosslinking between a CTS-HA composite membrane and Na2SO3. Among all the composites studied, CTS-HA (4/2 g/g) composite membrane is the best one for Guided Tissue Regeneration (GTR).
Astaxanthin: Senyawa Antioksidan Karoten Bersumber dari Biota Laut Munifah, Ifah; Wikanta, Thamrin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 1, No 1 (2006): December 2006
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.866 KB) | DOI: 10.15578/squalen.v1i1.71

Abstract

Meskipun berbagai jenis antioksidan dalam makanan telah berkontribusi dalam pencegahan penyakit, namun sejumlah riset yang telah dilakukan lebih memfokuskan pada tiga jenis antioksidan yakni vitamin E, vitamin C dan karotenoid karena berperan penting sebagai nutrien yang diperlukan dalam metabolisme tubuh manusia.
SYNTHESIS OF POLYETHYLENE OXIDE (PEO)–CHITOSAN HYDROGEL PREPARED BY GAMMA RADIATION TECHNIQUE Erizal, Erizal; Wikanta, Thamrin
Indonesian Journal of Chemistry Vol 11, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.292 KB) | DOI: 10.22146/ijc.21413

Abstract

Crosslinked PEO hydrogel containing chitosan for wound dressing has been prepared by gamma radiation technique. Chitosan solutions with different concentration (0.5-2% w/v) have been blended with 5% aqueous solution of PEO and irradiated at the doses 20-40 kGy by gamma rays. The copolymers were characterized by Fourier transform infra red spectroscopy and their physico-chemical properties of hydrogels were evaluated in terms of gel fraction, swelling ratio, elongation at break and antimicrobial activities. It was found that under maximum condition of incorporation 1% chitosan with irradiation dose of 20 kGy, PEO-chitosan hydrogels with high gel fraction (85%), swelling ratio (10 g/g) and elongation at break (145%) were obtained. The examination of the microbe penetration shows that the prepared hydrogels can be considered as a good barrier against microbes. Thus, PEO-chitosan hydrogel showed satisfactory properties for use as a wound dressing.
PENGARUH KOMBINASI PERLAKUAN KALIUM HIDROKSIDA DAN NATRIUM KARBONAT DALAM EKSTRAKSI NATRIUM ALGINAT TERHADAP KUALITAS PRODUK YANG DIHASILKAN Basmal, Jamal; Wikanta, Thamrin; Tazwir, Tazwir
Jurnal Penelitian Perikanan Indonesia Vol 8, No 6 (2002): (Vol.8 No.6 2002)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jppi.8.6.2002.45-52

Abstract

Penelitian ekstraksi sodium alginat (Na-alginat) telah dilakukan menggunakan rumput laut coklat Sargassum filipendula yang direndam di dalam larutan KOH: 0,25%, 0,50%, 0,75%, dan 1,0% dengan waktu perendaman setiap perlakuan 30 dan 60 menit.