Articles

Flower Characteristics and the Yield of Jatropha (Jatropha curcas L.) Accessions

HAYATI Journal of Biosciences Vol 16, No 4 (2009): December 2009
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The flower characteristics of a plant is one of the important traits correlated to its productivity. Study on flower characteristics is useful to understand how to increase the crop productivity. The research was conducted at Agro Techno Park Center, Indonesian State Ministry of Research and Technology, Bakung Village, Ogan Ilir Distric South Sumatra from April 2007 to August 2008.  The objective of this research was to evaluate the flower characteristics and the yield of jatropha (Jatropha curcas L.) accessions. The research was arranged in a randomized block design, consisted of fifteen accessions of jatropha, with three replicates. The Jatropha accessions were collected from different agro ecosystems located in Sumatra, Java, and Borneo. The results indicated that the evaluated accessions have significant differences in the flower characteristic and the yields. The ratio male–female flower is the most important character for J. curcas L. because it has a significant correlation to the yield.         Key words: flower, yield, Jatropha curcas

Evaluasi Penampilan dan Efek Heterosis Hasil Persilangan Beberapa Aksesi Jarak Pagar (Jatropha curcas L.)

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 41, No 1 (2013): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Agronomic performance and heterosis effect of progeny are important criteria for selection of parental hybrid. Theagronomic performance evaluation of Jatropha progeny which was derived from crossing of some Jatropha accessions hasbeen carried out for those goals. The study was conducted at Agro Techno Park, Indralaya South Sumatra. The crossingcombinations were Pidie x Medan, Curup x Lampung, Pidie x Lampung, Lampung x Pidie, Curup x Medan, Indralaya xPontianak, Medan x Palembang, Curup x Pidie, and Jogya x Komering. Oil production, nitrate reductase enzyme activity,leaf chlorophyll content and relative plant growth rate were evaluated. The result showed that the relative plant growth ratecould be used as early detection parameter for yield potential. Progenies of Pontianak x Indralaya, Palembang x Indralayaand Pidie x Lampung were selected as promising hybrids based on high productivity and heterosis effect.Keywords: Jatropha curcas, heterosis, relative plant growth rate, nitrate reductase activity

The Correlation Between Adiponectin and Tumor Necrosis Factor Alpha (TNF-a) in Indonesia Obese non Diabetic Males

Journal of the Indonesian Medical Association Vol. 61 No. 1 January 2011
Publisher : Journal of the Indonesian Medical Association

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Abstract

Adiponectin is an adipocytokine, abundant in the peripheral circulation, which has been shown to have anti-inflammatory and anti-atherogenic properties. Plasma concentration of adiponectin is decreased in obesity and negatively correlates with body mass index (BMI) and with insulin resistance. The aim of this study is to know adiponectin profile and its association with TNF-a in Indonesian obese non diabetic males. This is a cross sectional study with 137 Indonesian obese non diabetic males volunteers (age between 30-60 years, waist circumference >90 cm and fasting blood glucose <126 mg/dL) which was done in Jakarta, Indonesia. There is a significant negative correlation between adiponectin and TNF (r=-0.232; p=0.006) and nonsignificant negative correlations between adiponectin and fasting plasma glucose, adiponectin and HOMAIR, and adiponectin and waist circumference. This study confirm that central obesity will reduce adiponectin concentration so that increase insulin resistance and inflammation risk in Indonesian obese non diabetic men.Key words: Obesity, Adiponectin, TNF-a, Insulin Resistance, Inflammation

Disturbance of High Density Lipoprotein (HDL) Biogenesis in Men with Metabolic Syndrome

Journal of the Indonesian Medical Association Vol. 60 No. 8 August 2010
Publisher : Journal of the Indonesian Medical Association

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Abstract

High Density Lipoprotein cholesterol (HDL-c) has an  important antiatherogenic role through its reverse cholesterol transport (RCT) activity by stimulating  cholesterol efflux from peripheral tissue to the liver to be excreted through gallbladder. Biogenesis of HDL  involves factors that regulate the synthesis,  intravascular remodeling, and catabolism of HDL, including apolipoprotein A-1 (apoA-1), prebeta-1 HDL, and cholesteryl ester transfer protein (CETP).Disturbance of these factors can lead to low  concentrations of HDL-c. Metabolic syndrome(MetS) is characterized by low concentration of HDL-c. This study aimed to determine mechanisms of disturbance of HDL biogenesis factors in men with MetS that lead to low concentration of HDL-c. Using a cross-sectional design, this study involved 79 adult men, aged 25-60 years old with metabolic syndrome (IDF criteria 2005). This study was a comparative  observational study with cross-sectional design involving 70 subjects in the control group and 79 subjects in the MetS group (IDF criteria 2005). Subjects were men aged 25-60 years. Measured indicators were waist circumference (WC), blood pressure, fasting blood glucose (FBG), triglyceride, HDL-c, apoA-1,prebeta-1 HDL, and CETP. Data were analyzed using SPSS version 13 software. The results showed that there were significant differences in HDL biogenesis factors between the control group with SM groups, namely apoA1 (132.64+15.27 vs 119.08+19.72), prebeta-1 HDL (38 612.50+15 359.04 vs. 16 074.82+6 201.67), and CETP (2 364+0.442 vs 2 892+0.553). The study concluded that the disturbance of HDL biogenesis factors in MetS individual lead to lowconcentrations of HDL-c, namely decreased of  synthesis of HDL-c and increased catabolism of HDL-c.Keywords: ApoA-1, prebeta-1 HDL, CETP, HDL biogenesis and metabolic syndrome

Modifikasi Aplikasi Unsur Hara untuk Perbaikan Vigorasi Bibit Padi dalam Cekaman Terendam

Jurnal Lahan Suboptimal Vol 1, No 1 (2012)
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Suwignyo et al., 2012. Modification of Nutrient Application for the Improvement of Rice Seed Vigor in Submergence Threat. JLSO 1(1):1-11.In fresh water swamp rice cultivation, farmers  face the problem of high water levels during the early stage of plant growth, causing risky situation for seed. This study was aimed to obtain a method to increase rice seedling tolerance to submergence stress through improving fertilizer application  rice variety. The experimental design used was factorial completely randomized design with three replications. The factor consists of rice varieties (Inpara 3, Inpara 4, Inpara 5, BR 11 sub-1, IR 42, Rutti, Uffa, and Serendah Kuning), and fertilization treatment (NPK at planting without submergence; NPK with N at 7 days prior to submergence; NPK with N at one day after submergence; NPK + manure with N at 7 days prior to submergence; NPK + Manure + Si + Zn with N at 7 days prior to submergence; and NPK + Manure + Si + Zn with N at one day after submergence). The results showed that application of the proper nutrients could increase rice seedling vigor under submergence stress. At nursery stage, application of NPK, Manure, Si and Zn, with Nitrogen  after submergence would improve seedling vigor after recovery period. Submergence stress tolerant varieties showed higher ability to survive under submergence stress, and Inpara 4, Inpara 5, and BR11 Sub-1 performed better than Inpara 3. Serendah Kuning showed  better response to submergence stress than other local varieties. 

Identifikasi Beberapa Aksesi Jarak Pagar (Jatropha curcas L.) Melalui Analisis RAPD dan Morfologi

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 2 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this research was to study and cluster of Jatropha germplasm belonging to University of Sriwijaya.  This research was conducted from September 2007 until July 2008. The research used 14 accessions of Jatropha taken from some regions in Indonesia, namely: Komering, Palembang, Yogyakarta, Indralaya, ATP2, Pontianak, Lahat, Pagaralam, Curup, Lampung, Medan Aceh Besar, Pidi and Gorontalo.  Accessions of Jatropha curcas L. were planted at Agro Techno Park (ATP)  Bakung village, Indralaya Utara district Ogan Ilir, South Sumatera using Randomized Complete Block Design.  RAPD analysis using 20 primers was done  at RGCI (Research Group on  Crop Improvement), Bogor Agricultural University.  Dendrogram based on RAPD analysis  produced five groups that were: the first group was Komering, Lahat, Pidi, Indralaya, Aceh Besar, Pontianak and Curup.  The second group was Palembang and ATP2. The third group was Pagaralam, Gorontalo, and Medan.  Lampung was included in to fourth group.  The fifth group was Yogyakarta.  Dendrogram from morphological marker had also five groups.  First group was: Komering, Indralaya, Pontianak. Lahat, and Pagaralam.  Second group was: Palembang, Lampung, Pidi, Medan, and ATP2. Third group was: Curup. Fourth group was: Yogyakarta and Gorontalo.  Fifth group was: Aceh Besar. The difference of member from each groups between dendrogram using RAPD and morphological markers indicated that the bands resulted from RAPD did not have relation with characters observed.   Key words:  Jatropha curcas L, RAPD analysis, Morphologycal marker, cluster analysis

Waist Circumference was Positively Correlated with Chemerin, Retinol-Binding Protein 4 and hsCRP

The Indonesian Biomedical Journal Vol 4, No 1 (2012)
Publisher : The Prodia Education and Research Institute (PERI)

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Abstract

BACKGROUND: Central obesity is associated with various chronic metabolic disorders characterized by abnormal cytokine production, increased acute phase reactants, and activation of inflammatory signaling pathways. This study was aimed to investigate the association of waist circumference, chemerin, and retinol binding protein (RBP)-4 with inflammation in men with central obesity.METHODS: The research was conducted with a crosssectional design involving 68 centrally obese male subjects aged 30 to 60 years old, with waist circumference (WC) >90 cm. All subjects fulfilled the inclusion and exclusion criteria. Anthropometric parameters, fasting glucose, creatinine, SGOT, SGPT and hsCRP were measured. Serum concentrations of chemerin and RBP4 were measured by ELISA.RESULTS: The trend lines showed that chemerin, RBP4, and hsCRP increased with WC. Pearson correlation test showed a positively significant correlation between WC and hsCRP (r=0.242, p<0.05); and also between chemerin and hsCRP (r=0.244, p<0.05) and RBP4 (r=0.321, p<0.01). Subjects were stratified into four groups based on their chemerin and RBP4 levels (high chemerin/high RBP4, high chemerin/low RBP4, low chemerin/high RBP4, or low chemerin/low RBP4). Subjects who were in the high chemerin/low RBP4 group were more likely to have high level of inflammation (47.6%), but subjects with high chemerin/high RBP4 showed low level of inflammation (42.9%) as compared with the other three groups.CONCLUSIONS: We concluded that increased WC was correlated with elevated levels of chemerin, RBP4, and hsCRP. High chemerin was correlated with increased level of RBP4 as well as with high level of inflammation.KEYWORDS: waist circumference, chemerin, RBP4, hsCRP, inflammation

Metaflammation, NLRP3 Inflammasome Obesity and Metabolic Disease

The Indonesian Biomedical Journal Vol 3, No 3 (2011)
Publisher : The Prodia Education and Research Institute (PERI)

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Abstract

BACKGROUND: Increasing prevalence of obesity gives rise to many problems associated with multiple morbidities, such as diabetes, hypertension, heart disease, sleep apnea and cancer. The mechanism of obesity is very complex, thus its link to various disease is poorly understood. This review highlights important concepts in our understanding of the pathogenesis of obesity and related complications.CONTENT: Many studies have tried to explore the exciting and puzzling links between metabolic homeostasis and inflammatory responses. A form of subclinical, low-grade systemic inflammation is known to be associated with both obesity and chronic disease. This, later called as "metaflammation", refers to metabolically triggered inflammation. The nutrient-sensing pathway and the immune response coordination are facilitated by these molecular sites in order to maintain homeostasis under diverse metabolic and immune conditions. Recent studies have found that the NLRP3 inflammasome during metabolic stress forms a tie linking TXNIP, oxidative stress, and IL-1β production. This provides new opportunities for research and therapy for the disease often described as the next global pandemic: type 2 diabetes mellitus (T2DM).SUMMARY: The crucial role of metaflammation in many complications of obesity shown by the unexpected overlap between inflammatory and metabolic sensors and their downstream tissue responses. Then great interest arose to explore the pathways that integrate nutrient and pathogen sensing, give more understanding in the mechanisms of insulin resistance type 2 diabetes, and other chronic metabolic pathologies. A family of intracellular sensors called NLR family is a critical component of the innate immune system. They can form multiprotein complexes, called inflammasome which is capable of responding to a wide range of stimuli including both microbial and self molecules by activating the cysteine protease caspase-1, leading to processing and secretion of the proinflammatory cytokines IL-1β and IL-18, which play crucial roles in host defense. Inflammasome dysregulation has been linked to some autoinflammatory and metabolic diseases. These provide opportunities to continue to improve our understanding of the nature of metaflammation in the hope of modifying it to prevent and treat diseasese.KEYWORDS: Inflammation, metaflammation, inflammasome, metabolic disease, obesity

Adipose-Derived Stem Cells for Future Regenerative System Medicine

The Indonesian Biomedical Journal Vol 4, No 2 (2012)
Publisher : The Prodia Education and Research Institute (PERI)

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Abstract

BACKGROUND: The potential use of stem cell-based therapies for repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions for a number of disease. Despite the advances, the availability of stem cells remaining a challenge for both scientist and clinicians in pursuing regenerative medicine. CONTENT: Subcutaneous human adipose tissue is an abundant and accessible cell source for applications in tissue engineering and regenerative medicine. Routinely, the adipose issue is digested with collagenase or related lytic enzymes to release a heterogeneous population for stromal vascular fraction (SVF) cells. The SVF cells can be used directly or can be cultured in plastic ware for selection and expansion of an adherent population known as adipose-derived stromal/stem cells (ASCs). Their potential in the ability to differentiate into adipogenic, osteogenic, chondrogenic and other mesenchymal lineages, as well in their other clinically useful properties, includes stimulation of angiogenesis and suppression of inflammation.SUMMARY: Adipose tissue is now recognized as an accessible, abundant and reliable site for the isolation of adult stem cels suitable for the application of tissue engineering and regenerative medicine applications. The past decade has witnessed an explosion of preclinical data relating to the isolation, characterization, cryopreservation, differentiation, and transplantation of freshly isolated stromal vascular fraction cells and adherent, culture-expanded, adipose-derived stromal/stem cells in vitro and in animal models.KEYWORDS: adipose tissue, adult stem cells, regenerative medicine, mesenchymal stem cells

Pro-inflammatory Profiles of Indonesian Adult Men with Central Obesity: A Preliminary Study on TNF-alpha, sTNFR-2 and IL-1beta

The Indonesian Biomedical Journal Vol 2, No 1 (2010)
Publisher : The Prodia Education and Research Institute (PERI)

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Abstract

BACKGROUND: Central obesity is closely associated with chronic inflammation, characterized by abnormal cytokine production such as IL-1β, tumor necrosis factor-α (TNF-α) and tumor necrosis factor receptor-2 (TNFR-2). Central obesity and chronic inflammation form a complex link with insulin resistance, leading to the development of type-2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). Knowing the coinciding occurrence of chronic inflammation and central obesity, this study aimed to examine pro-inflammatory cytokine profiles of Indonesian adult men with central obesity.METHODS: This cross-sectional study recruited 80 apparently healthy Indonesian adult men, aged 23-53 years with waist circumference of 64-125 cm. This study was done in Jakarta. Measurements included clinical parameters like systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting blood glucose (FBG), aspartate amino transferase (AST), alanine amino transferase (ALT), creatinine, high sensitivity C-reactive protein (hsCRP), anthropometric parameters, namely weight, height and waist circumference; and pro-inflammatory cytokines TNF-α, soluble TNFR-2 (sTNFR-2) and IL-1β.RESULTS: Basic characteristics of the subjects showed linear increase in values of SBP, DBP and the serum concentrations of AST, ALT, FBG, GFR, hsCRP (p<0.005, respectively) with the degree of obesity. sTNFR-2 and IL-1 β positively correlated with hsCRP (r=0.277, p=0.013 and r= 0.257, p=0.022, respectively), WC (r=0.380, p=0.001 and r=0.400, p<0.001, respectively) and body mass index (BMI) (r=0.364, p=0.001 and r=0.399, p<0.001, respectively). Moreover, TNF-α did not show correlations with hsCRP, WC and BMI.CONCLUSION: There was a linear increase in the serum concentrations of sTNFR-2 and IL-1β in subjects with central obesity. Both pro-inflammatory markers, correlated with hsCRP, WC and BMI, but TNF-α did not. sTNFR-2 and IL-1β were, therefore, considered as valid biomarkers to indicate chronic inflammation in Indonesian adult men with central obesity.KEYWORDS: central obesity, TNF-α, soluble TNFR-2 (sTNFR-2), IL-1β

Co-Authors , Susantidiana . Khodijah, . . SUWANDI Adi, Nuri P. Ali Aspar Mappahya Allen Widyasanto, Allen Andi Asadul Islam Andi Asadul, Andi Andri Hidayat, Andri Anggi Kartikawati, Anggi Anna Meilana, Anna Anna Meiliana Anna Meilina, Anna Antonia Anna Lukito Anwar Santoso Anwar Susanto, Anwar Any Kusumastuti Arief Rachmawan, Arief As'ad, Suryani Astuti Kurnianingsih, Astuti As’ad, Suryani Bachtiar Murtala Bahtiar, Akhmad Seruji Bayu Winata Putera, Bayu Winata Benyamin Lakitan Bertha Pangaribuan Burhanuddin Bahar Cahyono Kaelan, Cahyono Candra Ninghayu Cynthia R. Sartika Cynthia Retna Sartika, Cynthia Retna Dewi Meidalima Dewi, Rafika Diah Kumalasari Dwi Yuniati Daulay, Dwi Yuniati Eka Armas Pailis Eli Halimah, Eli Erizal Sugiono, Erizal Erna Siaga, Erna Evriani Mareza, Evriani Evy Liswati, Evy Fatahillah, FNU Ferry Sandra Firdaus Sulaiman, Firdaus Gatot Lawrence, Gatot Gatot S. Lawrence Gatot Susilo Lawrence Gribaldi Gribaldi Harun Alrasyid Hendri Candro Nauli Manalu, Hendri Candro Nauli HENY HAWALID Hera Yuliana Intantri, Hera Yuliana Hesty Sihombing Husnul Hotimah, Husnul Ida P. Sormin Ida Paulina Sormin Ilhamjaya Patellongi Imelda Novianti, Imelda Indriyanti Rafi Sukmawati, Indriyanti Rafi Irawan Yusuf Irwan Suhendry, Irwan Joko Widodo Kadir, Sabaruddin Kartika Kartika Kurniawidjaja, Meily Kurniawidjaja Laela Hayu Nurani Laily Ilman Widuri, Laily Ilman Lazim, Farihin lhamjaya Patellongi Lies Gantini, Lies Lucia Herminawati, Lucia Lukman H Makmun Mansyur Arief, Mansyur Mansyur Arif Maria Diah Fibriani, Maria Diah Marita Kaniawati Marsetio Donosepoetro Meihana, Mei Meily L Kurniawidjaja, Meily L Meiriza Djohari, Meiriza Memen Surahman Mery Hasmeda Muchtaruddin Mansyur MUHAMAD UMAR HARUN Mulyana Mulyana Nelly Sari, Nelly Nuri Purwito Purwito Adi, Nuri Purwito Purwito Nurkhasa Nurkhasa, Nurkhasa Nurrani Mustika Dewi, Nurrani Mustika Patellongi, Ilham Jaya Peter Kabo Pratami, Vani N. Reni Suryanita Rina Triana, Rina Rinda Fajrin Aldina, Rinda Fajrin Rini Budiyati, Rini Rosalia E Napitupulu, Rosalia E Rujito Agus Suwignyo Rusli Muljadi, Rusli Samuel Oetoro Sanjaya, Nanda Sayurandi Sayurandi, Sayurandi Sholehah, Ari Fatus SITI HERLINDA Suryani As’ad Suryani As’ad Susana Gunawan, Susana SUSANTI DIANA Suwandi Suwandi Syakib Bakri Teguh A S Ranakusuma, Teguh A S Teguh A. S. Ranakusuma, Teguh A. S. Teguh A.S. Ranakusuma Teguh Santoso Terawan Agus Putranto, Terawan Agus Tommy Heryantho, Tommy Toti Indrawati Triani Adam Trilis Yulianti, Trilis Tugas Ratmono, Tugas Widjaja Lukito Widya Kurniawati, Widya Wiwik Rositawati, Wiwik Yan Sukmawan, Yan Yani Lina Yenny Surjawan Yulhasmir, Yulhasmir Yusmiati Yusmiati, Yusmiati Zainal Arifin Zainal Ridho Djafar Zulfikar Djauhari