Putu Aditya Wiguna
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Detection of HBV-DNA and Its Correlation with the HBeAg/Anti-HBe Serological Status in HBsAg-positive Patients Widita, Haris; Soemohardjo, Soewignjo; Muttaqin, Zainul; Wiguna, Putu Aditya; Rhamdiani, Shelly Olivia; Wijaya, Mahendra; Gunawan, Stephanus
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 2, August 2012
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Background: In the past years, HBeAg and anti-HBe status in individuals with positive HBsAg were often correlated to viral replication. This study was aimed to find correlation between the HBV viremia and HBeAg/anti-HBe serological status in HBsAg-positive individuals. Method: An observational-analytic design was performed in this study. The sera of all positive HBsAg patients at Biomedika Hospital Laboratory were collected and examined for HBeAg and anti-HBe using immunochromatography technique between January and April 2012. The sampling method was purposive sampling. Afterwards, the sera were examined for HBV-DNA by polymerase chain reaction (PCR). Results: Sufficient amount of sera were collected from 44 patients consisting of 33 males and 11 females. The mean age was 15-68 years. Positive HBeAg and negative anti-HBe status was found in 11 (42%) patients. Negative HBeAg and positive anti-HBe was found in 26 (59.1%) patients. Both HBeAg and anti-HBe were negative in 7 (16.3%) patients. HBV-DNA was detected in all 11 (100%) patients with positive HBeAg and negative anti-HBe. HBV-DNA was also detected in 11 (42%) patients with negative HBeAg and positive anti-HBe. However, there was only one patient (14.3%) with both negative HBeAg and anti-HBe status, who had detectable HBV-DNA. Conclusion: Positive HBeAg can be used as an indicator of viremia, but negative HBeAg cannot be used as an indicator of the absence of viremia without further HBV-DNA testing. Patients with negative HBeAg and positive HBV-DNA were suspected for having pre-core mutant. Keywords: HBV-DNA, positive HBsAg, HBeAg, anti-HBe, pre-core mutant
Detection of Hepatitis B Virus Pre-core Mutant by Allele Specific Polymerase Chain Reaction Soemohardjo, Soewignjo; Widita, Haris; Muttaqin, Zainul; Gunawan, Stephanus; Wijaya, Mahendra; Wiguna, Putu Aditya; Rhamdiani, Shelly Olivia
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 14, NUMBER 1, April 2013
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Introduction: Mutation in pre-core region is characterized by negative HBeAg and positive anti-HBe despite active replications of the virus. The mutation has diagnostic and prognostic implications. Therefore, detectionof pre-core mutant is important. Standard diagnosis approach for detecting pre-core mutant is through DNA sequencing of hepatitis B virus (HBV) pre-core region. Unfortunately, DNA sequencing is not available in mostcenters. Hence, a simpler diagnostic approach is necessary.Method: An observational-analytic design study was performed. Detection of pre-core mutant was conducted in individuals with positive HBsAg and HBV DNA that had various patterns of HBeAg and anti HBe. HBsAg, HBeAg and anti-HBe was detected using immunochromatography technique. The HBV DNA was evaluated by using qualitative polymerase chain reaction (PCR) testing. PCR was done by three rounds of amplification with primers derived from wild type pre-core and mutant pre-core. Results: Of 25 sera with HBeAg negative, anti-HBe positive and HBV DNA positive, allele specific (AS) PCR pre-core mutant was detected in 20 (80%) sera. Two sera with HBeAg negative, anti HBe negative and HBV DNA positive were negative for pre-core mutant. Of 8 sera with HBeAg positive, anti HBe negative and HBV DNA positive, pre-core mutant was detected in 2 (25%) sera.Conclusion: Most of individuals with HBV DNA positive, HBeAg negative and anti-HBe positive have harbored pre-core mutant. The finding indicated that all patients with HBsAg positive, HBV DNA positive and HBeAg negative, but anti-HBe positive should be examined for the presence of pre-core mutant. Pre-core mutant is also found in HBeAg positive individual. Keywords: HBV, pre-core mutant, polymerase chain reaction
Detection of Hepatitis B Virus Pre-core Mutant by Allele Specific Polymerase Chain Reaction Soemohardjo, Soewignjo; Widita, Haris; Muttaqin, Zainul; Gunawan, Stephanus; Wijaya, Mahendra; Wiguna, Putu Aditya; Rhamdiani, Shelly Olivia
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 14, NUMBER 1, April 2013
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/141201319-23

Abstract

Introduction: Mutation in pre-core region is characterized by negative HBeAg and positive anti-HBe despite active replications of the virus. The mutation has diagnostic and prognostic implications. Therefore, detectionof pre-core mutant is important. Standard diagnosis approach for detecting pre-core mutant is through DNA sequencing of hepatitis B virus (HBV) pre-core region. Unfortunately, DNA sequencing is not available in mostcenters. Hence, a simpler diagnostic approach is necessary.Method: An observational-analytic design study was performed. Detection of pre-core mutant was conducted in individuals with positive HBsAg and HBV DNA that had various patterns of HBeAg and anti HBe. HBsAg, HBeAg and anti-HBe was detected using immunochromatography technique. The HBV DNA was evaluated by using qualitative polymerase chain reaction (PCR) testing. PCR was done by three rounds of amplification with primers derived from wild type pre-core and mutant pre-core. Results: Of 25 sera with HBeAg negative, anti-HBe positive and HBV DNA positive, allele specific (AS) PCR pre-core mutant was detected in 20 (80%) sera. Two sera with HBeAg negative, anti HBe negative and HBV DNA positive were negative for pre-core mutant. Of 8 sera with HBeAg positive, anti HBe negative and HBV DNA positive, pre-core mutant was detected in 2 (25%) sera.Conclusion: Most of individuals with HBV DNA positive, HBeAg negative and anti-HBe positive have harbored pre-core mutant. The finding indicated that all patients with HBsAg positive, HBV DNA positive and HBeAg negative, but anti-HBe positive should be examined for the presence of pre-core mutant. Pre-core mutant is also found in HBeAg positive individual. Keywords: HBV, pre-core mutant, polymerase chain reaction
Detection of HBV-DNA and Its Correlation with the HBeAg/Anti-HBe Serological Status in HBsAg-positive Patients Widita, Haris; Soemohardjo, Soewignjo; Muttaqin, Zainul; Wiguna, Putu Aditya; Rhamdiani, Shelly Olivia; Wijaya, Mahendra; Gunawan, Stephanus
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 2, August 2012
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/132201286-89

Abstract

Background: In the past years, HBeAg and anti-HBe status in individuals with positive HBsAg were often correlated to viral replication. This study was aimed to find correlation between the HBV viremia and HBeAg/anti-HBe serological status in HBsAg-positive individuals. Method: An observational-analytic design was performed in this study. The sera of all positive HBsAg patients at Biomedika Hospital Laboratory were collected and examined for HBeAg and anti-HBe using immunochromatography technique between January and April 2012. The sampling method was purposive sampling. Afterwards, the sera were examined for HBV-DNA by polymerase chain reaction (PCR). Results: Sufficient amount of sera were collected from 44 patients consisting of 33 males and 11 females. The mean age was 15-68 years. Positive HBeAg and negative anti-HBe status was found in 11 (42%) patients. Negative HBeAg and positive anti-HBe was found in 26 (59.1%) patients. Both HBeAg and anti-HBe were negative in 7 (16.3%) patients. HBV-DNA was detected in all 11 (100%) patients with positive HBeAg and negative anti-HBe. HBV-DNA was also detected in 11 (42%) patients with negative HBeAg and positive anti-HBe. However, there was only one patient (14.3%) with both negative HBeAg and anti-HBe status, who had detectable HBV-DNA. Conclusion: Positive HBeAg can be used as an indicator of viremia, but negative HBeAg cannot be used as an indicator of the absence of viremia without further HBV-DNA testing. Patients with negative HBeAg and positive HBV-DNA were suspected for having pre-core mutant. Keywords: HBV-DNA, positive HBsAg, HBeAg, anti-HBe, pre-core mutant
Faktor yang Berhubungan dengan Pemberian ASI Eksklusif pada Ibu Bekerja di Kota Mataram Wiguna, Putu Aditya; Yuliani, Eka Arie; Affarah, Wahyu Sulistya; Reditya, Ni Made
Jurnal Kedokteran Vol 5 No 4 (2016)
Publisher : Faculty of Medicine Universitas Mataram

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Pendahuluan: ASI eksklusif berdampak luas pada status gizi dan kesehatan balita. Angka keberhasilan pemberian ASI eksklusif pada ibu bekerja sangat rendah. Tujuan: Menentukan faktor-faktor yang berhubungan dengan pemberian ASI eksklusif pada ibu bekerja di Kota Mataram. Metode: Penelitian deskriptif analitik dengan rancangan potong lintang menggunakan kuisioner dilakukan sepanjang Desember 2013. Responden merupakan ibu bekerja di Kota Mataram, memiliki bayi berusia 6-36 bulan, menyusui, bayi juga tidak memiliki kelainan kongenital atau penyakit kronis. Hasil: Dari 288 ibu yang diwawancarai, 135 (46,9%) memberikan ASI eksklusif. Odd ratio menyusui dengan ASI eksklusif lebih tinggi pada ibu yang mendapat dukungan suami (OR = 21,32, CI = 2,28-160,77, p = 0,000), memiliki sarana dan prasarana menyusui di tempat kerja (OR=1,91, CI = 1,12-3.26, p = 0,017), memiliki durasi perjalanan ke tempat kerja kurang dari 15 menit (OR=1,93, CI = 1,20-3,11, p=0,006) dan menyiapkan ASI perah (OR = 2,45, CI = 1,52-3,95, p=0,000). Paparan terhadap saran untuk memberikan makanan atau minuman lain selain ASI pada bayi berumur kurang dari 6 bulan, lamanya jam kerja, jabatan ibu di tempat kerja, dan lamanya cuti menyusui tidak berhubungan dengan keberhasilan pemberian ASI eksklusif (p>0,05). Kesimpulan: Keberhasilan menyusui dengan ASI Eksklusif pada ibu bekerja berhubungan dengan dukungan suami, ketersediaan sarana dan prasarana menyusui di tempat kerja, durasi perjalanan ke tempat kerja kurang dari 15 menit dan menyiapkan ASI perah.
PERBANDINGANEFEKPEMBERIANAIR KELAPA MUDADAN AIR PUTIHTERHADAPKECEPATANPEMULIHAN DENYUT NADIPADA PEMAIN FUTSAL FK UNRAM Widiastuti, Ida Ayu Eka; Wiguna, Putu Aditya
Jurnal Kedokteran Vol 2 No 2 (2013)
Publisher : Faculty of Medicine Universitas Mataram

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In physical activities, oxygen consumption, heart rate, body temperature and chemical compound in human body will alter transformation. Indirect method to measure workload can be done by measuring pulse during activities. Fluid loss in activities can be replaced by administering fluid that have good rehydration effect, among those are coconut water and drinking water. The aim of this study is to compare the effect of coconut water and drinking water consumption on pulse recovery time after exercise. This experimental study was conducted with randomized pre and posttest group design. Subject of this study is Mataram University Faculty of Medicine futsal team. Fourteen subjects were divided into two groups, each group consist of seven individuals. Coconut water was given to group 1 and drinking water was given to group 2 after treadmill exercise using Bruce Protocol for 15 minutes. The data were analyzed by using MannWhitney Test. Pulse recovery time average of group 1 that consumes coconut water was 157, 29 seconds or 2 minutes 62 seconds, while group 2 that consumes drinking water average was 282,86 seconds or 4 minutes 71 seconds. Mann-Whitney analysis shows that pulse recovery times after treadmill exercise were not significantly different (p > 0.05).Pulse recovery time average on group that consumed coconut water were better (28.5%) than group that consumed drinking water, but were not significantly different (p > 0.05). Keywords :Treadmill exercise, recovery time, coconut water, drinking water, futsal player
PROFIL ANEMIA BERDASARKAN INDEKS ERITROSIT PADA SISWA SMPN 2 KECAMATAN KHAYANGAN KABUPATEN LOMBOK UTARA Kurniawan, Moh. Arif; Lestarini, Ima Arum; Wiguna, Putu Aditya
Jurnal Kedokteran Vol 3 No 1 (2014)
Publisher : Faculty of Medicine Universitas Mataram

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Latar belakang: Anemia adalah penurunan volume eritrosit atau kadar hemoglobin (Hb) hingga dibawah rentang yang berlaku untuk orang sehat. Peningkatan atau penurunan kejadian anemia pada suatu daerah dapat dipengaruhi oleh kondisi demografi dan geografis suatu daerah. Kecamatan Kayangan memiliki kondisi geografis berupa dataran tinggi dan pantai. Mayoritas penduduknya berprofesi sebagai nelayan dan petani. SMPN 2 Kayangan merupakan salah satu sekolah tingkat menengah pertama yang berlokasi di Kecamatan Kayangan, yang mayorits siswanya tingg al di daerah dataran tinggi dan perkebunan. Penelitian ini dilakukan untuk mengetahui profil anemia berdasarkan indeks eritrosit yang paling banyak terjadi pada siswa SMPN 2 Kayangan. Metode: Rancangan penelitian ini mengunakan studi cross sectional. Teknik pengambilan sampel mengunakan sistem total samplingdari 222 siswa, namun responden yang memuhi kriteria inklusi sebanyak 196 siswa dan data diolah dengan metode analisis univariat. Hasil : Dari 196 responden, 24 (12,2%) siswa dinyatakan anemia berdasarkan nilai patokan Hb populasi (12,97 gr/dl?1,05), anemia paling banyak pada kelompok usia 13-14 tahun sebanyak 15 (62,5%) siswa, berjenis kelamin perempuan sebanyak 15 (62,5%) siswa, dengan status IMT < 18,5 sebanyak 16 (66,7%) siswa. Berdasarkan indeks eritrositnya, kejadian anemia mikrositik hipokromik sebanyak 15 (62,5%) siswa, anemia normositik hipokromik sebanyak 7 (29,2%) siswa dan anemia normositik normokromik sebanyak 2 (8,3%) siswa. Kesimpulan: anemia terbanyak adalah anemia mikrositik hipokromik sebanyak 15 (62,5%) siswa, diikuti anemia normositik hipokromik sebanyak 7 (29,2%) siswa dan anemia normositik normokromik sebanyak 2 (8,3%) siswa, dengan nilai rerata Hb12,97 gr/dl ?1,05. Kejadian anemia didominasi oleh kelompok usia 13-14 tahun berjenis kelamin perempuan dengan IMT < 18,5. Kata kunci: anemia, indeks eritrosit, siswa SMP
PERBANDINGAN EFEK PEMBERIAN AIR PUTIH DAN MINUMAN ISOTONIK TERHADAP KECEPATAN PEMULIHAN DENYUT NADI PADA MAHASISWA FAKULTAS KEDOKTERAN UNIVERSITAS MATARAM Putra, Kadek Soga Prayaditya; Widiastuti, Ida Ayu Eka; Wiguna, Putu Aditya
Jurnal Kedokteran Vol 3 No 1 (2014)
Publisher : Faculty of Medicine Universitas Mataram

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Latar Belakang: Saat melakukan latihan fisik terjadi peningkatan produksi keringat.Selama berkeringat tubuh akan kehilangan cairan dan elektrolit. Minuman isotonik adalah minuman dengan konsentrasi yang sama dengan cairan tubuh, sehingga dapat dengan cepat menggantikan kehilangan cairan dan elektrolit tubuh setelah beraktivitas. Tujuan dari penelitian ini adalah membandingkan efek pemberian air putih dan minuman isotonik terhadap kecepatan pemulihan denyut nadi. Metode:Rancangan penelitian ini adalah eksperimental pretest and posttest group design.Subjek penelitian adalah 25 orang Mahasiswa Fakultas Kedokteran Universitas Mataram,yang dipilih secarasimple random sampling. Sebelum melakukan latihan treadmill menggunakan protokol Bruce modifikasi selama 15 menit, subjek diberikan air putih dan minuman isotonik. Rentang waktu antara pemberian air putih dan minuman isotonik adalah 2 hari. Data dianalisis menggunakan uji Wilcoxon. Hasil: Berdasarkan hasil analisis data didapatkan bahwa rerata kecepatan pemulihan denyut nadi setelah pemberian air putih adalah 6,16 menit dan minuman isotonik adalah 4, 04 menit. Hasil uji rerata beda kecepatan pemulihan denyut nadi dengan metode Wilcoxon diperoleh hasil p < 0,05. Simpulan: Rerata kecepatan pemulihan denyut nadi setelah pemberian minuman isotonik lebih cepat jika dibandingkan dengan kecepatan pemulihan denyut nadi setelah pemberian air putih. Terdapat perbedaan bermakna efek pemberian air putih dan minuman isotonik terhadap rerata kecepatan pemulihan denyut nadi (p < 0,05). Kata Kunci: Latihan treadmill, kecepatan pemulihan denyut nadi, minuman isotonik, air putih, protokol Bruce modifikasi
Deskripsi Tingkat Pengetahuan dan Perilaku Pencegahan terhadap Malaria pada Siswa Sekolah Dasar di Daerah Non Endemis Malaria di Lombok Suryani, Dewi; Cenderadewi, Mutia; Wiguna, Putu Aditya; Rahman, Hadian; Fathana, Prima Bella
Jurnal Kedokteran Vol 6 No 2 (2017)
Publisher : Faculty of Medicine Universitas Mataram

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Latar Belakang: Malaria merupakan mosquito borne-disease yang masih menjadi permasalahan global. Banyak penelitian yang mendukung anak sekolah sebagai agen perubahan karena memiliki potensi untuk menyebarkan pesan kesehatan kepada anak yang lain, anggota keluarga, dan masyarakat. Penelitian ini merupakan penelitian lanjutan setelah sebelumnya telah dilakukan penelitian serupa di daerah endemis malaria di Lombok Timur. Penelitian ini bertujuan untuk mengetahui tingkat pengetahuan terkait malaria, sumber informasi tentang malaria serta perilaku dan penggunaan anti-nyamuk terkait penularan malaria pada siswa sekolah dasar di Mataram. Metode: Metode yang digunakan dalam penelitian ini adalah deskriptif observational dengan desain cross sectional. Populasi penelitian ini adalah siswa kelas 5 dan 6 di 4 Sekolah Dasar (SD) di Kota Mataram. &nbsp;Hasil: Tedapat 128 siswa yang terlibat dalam penelitian ini. Sebesar 92% siswa pernah mendengar istilah malaria dan sumber informasi utama mengenai sakit malaria adalah dari orang tua (46%). Didapat hanya 21,1% siswa yang mempunyai tingkat pengetahuan baik terhadap malaria. Aspek pengetahuan mengenai malaria yang perlu ditingkatkan adalah 1) pendapat mengenai sakit malaria; 2) gejala sakit malaria; 3) penyebab sakit malaria; 4) waktu nyamuk menggigit dan 5) cara pencegahan sakit malaria untuk diri sendiri. Ibu adalah anggota keluarga yang bertanggungjawab menyediakan metode pencegahan gigitan nyamuk di rumah. Sebagian besar rumah tangga menggunakan semprotan pembasmi nyamuk untuk menghindari gigitan nyamuk. Berdasarkan hasil penelitian ini, perlu dilakukan upaya intervensi berbasis sekolah untuk meningkatkan pengetahuan anak SD mengenai malaria. Kesimpulan: Pengetahuan anak SD di Kota Mataram tentang malaria sebagian besar tergolong sedang dan baik. Pencegahan malaria terutama dilakukan oleh ibu dengan menggunakan obat nyamuk semprot.
Faktor-faktor yang Mempengaruhi Keberhasilan Pemberian ASI Eksklusif di Kota Mataram Rahmadhona, Devi; Affarah, Wahyu Sulistya; Wiguna, Putu Aditya; Reditya, Ni Made
Jurnal Kedokteran Vol 6 No 2 (2017)
Publisher : Faculty of Medicine Universitas Mataram

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Latar Belakang: Tingkat pemberian ASI eksklusif di indonesia masih sangat rendah. Data dari Dinas Kesehatan Provinsi NTB mengenai cakupan pemberian ASI eksklusif belum memuaskan. Rendahnya pemberian ASI merupakan ancaman bagi tumbuh kembang anak yang akan berpengaruh pada pertumbuhan dan pengembangan kualitas SDM. Tujuan penelitian adalah untuk mengetahui faktor-faktor yang mempengaruhi keberhasilan pemberian ASI eksklusif di Kota Mataram. Metode: Metode penelitian yang digunakan adalah desktiptif analitik dengan desain potong lintang menggunakan kuisioner selama bulan Agustus hingga September 2014 pada ibu dengan bayi berumur 6-12 bulan yang tidak memiliki kelainan kongenital atau penyakit kronis. Hasil: Sebanyak 301 responden diwawancarai, sebanyak 198 orang (65,8%) memberikan ASI eksklusif. Faktor sosiodemografi yang memiliki hubungan bermakna dengan keberhasilan pemberian ASI eksklusif adalah pekerjaan ibu dengan tingkat korelasi sangat lemah (r=0,156,p=0,007). Faktor pra dan pasca natal yang memiliki hubungan bermakna dengan korelasi sangat kuat adalah pemberian MPASI (r=-0,844,p=0,000). Faktor psikososial yang memiliki hubungan bermakna dengan tingkat korelasi lebih tinggi dibanding faktor lainnya adalah keyakinan ibu untuk dapat menyusui (r=0,527,p=0,000) dan persepsi ibu terhadap kepuasan bayi saat menyusui (r=0,468,p=0,000). Kesimpulan: Sebanyak 65,8% ibu di Kota Mataram berhasil memberikan ASI eksklusif. Faktor sosiodemografi, faktor psikososial, faktor pra dan pasca natal memiliki hubungan bermakna dengan keberhasilan pemberian ASI eksklusif.