Articles

Found 22 Documents
Search

Comparison of Cytokine Profile between Indonesian Thin-Tailed and Merino Sheep during A Primary Infection with Fasciola gigantica Wiedosari, Ening; Piedrafita, David
Jurnal Veteriner Vol 13, No 1 (2012)
Publisher : Jurnal Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study was to investigate the expression of cytokines profiles interferon-? (IFN-?),interleukin-5 (IL-5) and IL-10 in Rasciola gigantica resistant Indonesian thin-tailed (ITT) sheep comparedto susceptible merino sheep infected with Fasciola gigantica. A total of ten ITT and merino sheep wererandomly allocated into infected (n=5) and control (n=5) groups, sheep were infected with 250 viablemetacercariae of F. gigantica. The cytokines were determined by isolated mRNA from hepatic lymph nodeby semi-quantitative RT-PCR (Reverse Trancriptase -Polymerase Chain Reaction). The result showedITT sheep produced significantly higher IL-5 and 10 ( P<0,05 ) than merino sheep, while ITT sheepproduced less IFN-? ( P<0,05) than merino sheep at 10 weeks post infection. It could be concluded thatmerino sheep tend to develop T type 1 cells, while the ITT sheep tend to develop T type 2 cells whicheffectively killed F. gigantica.
The susceptibility differences of buffalo and Ongole calves against trickle infection with Fasciola gigantica Wiedosari, Ening; Widjajanti, S; Partoutomo, S
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 1 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.133 KB) | DOI: 10.14334/jitv.v3i1.95

Abstract

A pen trial was carried out in order to determine the susceptibility differences of a trickle infection with Fasciola gigantica in buffalo and Ongole calves. Treated animals were infected orally with 15 metacercariae of F. gigantica twice weekly for 32 weeks and were slaughtered at 36 weeks. The results showed that buffalo calves had significantly lower fluke burdens than Ongole calves (P<0 .01) . All of the infected Ongole calves had fasciola eggs in their faeces 18 weeks after the commencement of infection. In contrast, eggs were detected only in 3 out of 7 infected buffalo calves at week 20, in 4 at week 28 and in 6 at week 30. Faeces of the seventh buffalo remained free of eggs until week 36. Rates of growth were reduced by 25%and 10,3% in infected Ongole and buffalo calves respectively . Circulating blood eosinophilia ofboth hosts, ahallmark of helminth infections, increased following infection, but values in buffaloes was greater than Ongole calves mainly in week 4 and 8 after infection (P<0.01). These results might be concluded that the susceptibility of buffalo calves to trickle infection with F. gigantica was lower compared to Ongole calves.   Keywords : Fasciola gigantica; susceptibility, buffalo calves, Ongole calves
Pathology and clinicopathology of buffalo against trickle infection with Fasciola gigantica Wiedosari, Ening
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 2 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v3i2.107

Abstract

Eleven male buffalo calves were divided into infected group of 7 animals and non-infected control group of 4 animals. The infected group was then inoculated with trickle doses of 15 Inetacercariae of Fasciola gigantica twice weekly for 32 weeks and killed 36 weeks after first infection. There were no clinical symptoms observed . Infected and non-infected buffaloes, had similar values of packed cell volume, haemoglobin and red blood cell counts . In infected buffaloes, plasma glutamate dehydrogenase enzyme activity increased in proportion to the dregree of hepatocyte destruction level and evidence of necrosis caused by the migrating of immature flukes through the parenchyma prior to their entry into the bile ducts. While the values of plasma glutamyl transpeptidase showed only a minimal rise with a small peak in week 20 as evidenced by histological observation that infected caused limited damage to epithelial surface of the bile duct . These results indicates that, the resistance mechaninisms of buffalo against fasciolosis infection occurred in the liver or before flukes entering into the bile ducts. These results seem to indicate that, in buffalo, resistance mechanisms to fasciolosis infection occured in the liver or before flukes entry into the bile ducts.   Key words: Fasciola gigantica, clinicopathology, buffalo calves, trickle infection, resistance
Role of Natural Immunomodulator (Aloe Vera) in Cellular and Humoral Immune Responses Wiedosari, Ening
Indonesian Bulletin of Animal and Veterinary Sciences Vol 17, No 4 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/wartazoa.v17i4.871

Abstract

Aloe vera belongs to a group of Liliaceae family plant and cultivated worldwide. It possesses acemannan (acetylated mannan), which has a significant pharmacological property. The acemannan has an immunomodulatory activity when administered to animals. The major immunomodulating effect includes the activation of immune effector cells, such as lymphocytes and macrophages, resulting in the production of cytokines, interleukin (IL)-1, IL-6, IL-12 and tumor necrosis factor alpha (TNFα). In particular, this extract can modulate the differentiation capacity of CD4+T cells to mature into Th1 subsets and enhance the innate cytokine response. As a consequence, this extract will have a profound effect in controlling disease, caused by intracellular infectious agents (bacteria and viruses). However, further studies are needed to determine the immunomodulating effects of Aloe vera in multi-component extracts equivalent to what are being  used commonly in traditional medicine.   Key words: Aloe vera, immunomodulator, cytokines
Infection of Parainfluenza type 3 (PI-3) as one of the causative agent of pneumonia in sheep and goats Sendow, Indrawati; Syafriati, Tatty; Wiedosari, Ening; Selleck, Paul
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 1 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v7i1.276

Abstract

Serological survey was conducted to obtain the prevalence of Parainfluenza type 3 (PI-3) reactor as one of the causative agent of pneumonia in sheep and goats in abatoir at Jakarta and some small holder farms in Indonesia. Serological test using serum neutralization from 724 goat sera and 109 sheep sera indicated that only 1% of goats were serologically reactors and none of sheep sera had antibodies against PI-3 virus. Isolation of the virus from 56 bronchus and trachea swab and 345 lungs indicated that only one sampel from lung showed cythopathic effect (CPE) in Madin Darby Bovine Kidney (MDBK) cell lines identification of the virus using serum neutralization test indicated that the virus neutralized reference PI-3 antisera. The isolate came from one lung (7%) of 24 that showed histopathologically pneumonia intertitialis that usually caused by viral infection. Key words: Parainfluenza type 3 (PI-3), virus isolation, sheep, goat, pneumonia intertisialis
The susceptibility differences of buffalo and Ongole calves against trickle infection with Fasciola gigantica Wiedosari, Ening; Widjajanti, S; Partoutomo, S
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v3i1.95

Abstract

A pen trial was carried out in order to determine the susceptibility differences of a trickle infection with Fasciola gigantica in buffalo and Ongole calves. Treated animals were infected orally with 15 metacercariae of F. gigantica twice weekly for 32 weeks and were slaughtered at 36 weeks. The results showed that buffalo calves had significantly lower fluke burdens than Ongole calves (P<0 .01) . All of the infected Ongole calves had fasciola eggs in their faeces 18 weeks after the commencement of infection. In contrast, eggs were detected only in 3 out of 7 infected buffalo calves at week 20, in 4 at week 28 and in 6 at week 30. Faeces of the seventh buffalo remained free of eggs until week 36. Rates of growth were reduced by 25%and 10,3% in infected Ongole and buffalo calves respectively . Circulating blood eosinophilia ofboth hosts, ahallmark of helminth infections, increased following infection, but values in buffaloes was greater than Ongole calves mainly in week 4 and 8 after infection (P<0.01). These results might be concluded that the susceptibility of buffalo calves to trickle infection with F. gigantica was lower compared to Ongole calves.   Keywords : Fasciola gigantica; susceptibility, buffalo calves, Ongole calves
The activities of antioxidant enzymes extracted from Fasciola gigantica infecting Thin Tailed and Merino sheep Wiedosari, Ening
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v11i2.520

Abstract

Previous studies shown that Indonesian Thin Tailed (ITT) sheep are more resistant to Fasciola gigantica infection than Merino sheep. This difference could be mediated by intrinsic defense enzymes of the parasite. Certain enzymes are known to be crucial in parasite survival against host-derived immune responses. We measured some of them to identify if any comparative differences between the enzyme activities of the parasites from the two hosts (ITT & Merino sheep) could account for the mechanisms of parasite resistance to killing by the Merino host and susceptibility to killing by the ITT host. Parasites were extracted from the liver of infected ITT and Merino sheep and superoxide dismutase (SOD), glutathione S-transferase (GST) and catalase (CAT) enzyme activities were assayed. SOD and GST levels were found to be higher in parasites isolated from Merino than those of ITT sheep (P<0.05), CAT activity was not detected in any of the parasites. There was significantly higher eosinophils (P<0,05) in the ITT sheep peritoneal cells. These results suggested that SOD dan GST are important molecules in determining susceptibility in Fasciola-infected Merino sheep and resistance in Fasciola-infected ITT sheep. Key Words: Fasciola Gigantica, Indonesian Thin Tailed Sheep, Merino Sheep, Superoxide Dismutase (SOD), Glutathione S-Transferase (GST)
Role of Natural Immunomodulator (Aloe Vera) in Cellular and Humoral Immune Responses Wiedosari, Ening
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 17, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.295 KB) | DOI: 10.14334/wartazoa.v17i4.871

Abstract

Aloe vera belongs to a group of Liliaceae family plant and cultivated worldwide. It possesses acemannan (acetylated mannan), which has a significant pharmacological property. The acemannan has an immunomodulatory activity when administered to animals. The major immunomodulating effect includes the activation of immune effector cells, such as lymphocytes and macrophages, resulting in the production of cytokines, interleukin (IL)-1, IL-6, IL-12 and tumor necrosis factor alpha (TNFα). In particular, this extract can modulate the differentiation capacity of CD4+T cells to mature into Th1 subsets and enhance the innate cytokine response. As a consequence, this extract will have a profound effect in controlling disease, caused by intracellular infectious agents (bacteria and viruses). However, further studies are needed to determine the immunomodulating effects of Aloe vera in multi-component extracts equivalent to what are being  used commonly in traditional medicine.   Key words: Aloe vera, immunomodulator, cytokines
PENGARUH JAMU HERBAL SEBAGAI ANTIKOKSIDIA PADA AYAM PEDAGING YANG DIINFEKSI Eimeria tenella WIEDOSARI, ENING; SUHIRMAN, SHINTA; SEMBIRING, BAGEM BR
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK
STUDI KOMPARATIF RESISTENSI ANTARA SAPI BALI DAN MADURA TERHADAP INFEKSI Fasciola gigantica Wiedosari, Ening
Widyariset Vol 13, No 2 (2010): Widyariset
Publisher : LIPI-Press

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Designing of future approaches for the control of Fasciola gigantica infection in cattle requires an understanding of the host-parasite relationships. This study was therefore undertaken to compare the susceptibility to infection with F. gigantica between Bali and Madura cattle. Seven Bali and seven Madura cattle were infected orally with 15 metacercariae of F. gigantica twice weekly for 32 weeks. Similar observations were made on four Bali and 4 Madura cattle maintained fluke-free as controls. The study shows that there was a trend of a lower fluke burden and faecal egg counts Madura cattle than in Bali cattle. The packed cell volume (PCV) values was significantlyhigher in infected Madura than in Bali cattle (P<0,05). The increased of eosinophil cell response and the antibody isotypes imunoglobulin (Ig)G1 in Madura cattle were significantly higher than in Bali cattle (P<0,05). These varying responses represent differences in host-parasite relationships between Bali and Madura cattle and may be linked to the observed varying levels of resistance to F. gigantica infection between these host.