Nyoman Suci Widyastiti
4Bagian Patologi Klinik Fakultas Kedokteran UNDIP Semarang

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HUBUNGAN ANTARA EKSKRESI IODIUM URIN DAN EKSKRESI TIOSIANAT URIN DENGAN TOTAL GOITER RATE Studi pada Anak SD di Kecamatan Bulakamba, Kabupaten Brebes Jawa Tengah

MEDIA MEDIKA MUDA 2012:MMM VOLUME 1 NUMBER 1 YEAR 2012
Publisher : MEDIA MEDIKA MUDA

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Background: Health issue become a serious problem in Indonesia such as Iodine Deficiency Disorders (IDD) nowadays is more attended because of the effects i.e. goitre. Goitre is the one of several IDD spectrums. Iodine deficiency is not the only affector of IDD. The others like goitrogenic substances, for the example is thiocyanate can be a competitor with iodine in the tyroid hormone synthesis. The levels of endemicities for certain area is assessed based on the size alteration of tyroid gland (TGR). Urine Excretion Iodine can discribe the iodine intake by person. The most thiocyanate excretion is by the kidney, so that the levels can be checked in urine. It is important to know whether goitre is affected by iodine defficiency or thiocyanate consumption. Therfore this research aims to determine the relationship between UEI, Urine Thiocyanate Excretion and TGR.Methode : This was an analytical observational research with cross sectional design. Samples were 67 respondens. Simple random sampling were done with them. TGRs were secondary data from Dinas Kesehatan of Brebes Regency in 2010/2011. EIU was measured with Acid Digestion Methode with persultafe ammonium. Urine Thiocyanate Excretion was measured with spectrophotometri methode. The data were subjected to statistical analysis for correlation at 5% level of significance and regression, using SPSS 15.0 for WindowsResults : UEI levels were 192,00 + 349 (346,00) μg/L. Urine Thiocyanate Levels were 0,36 + 4,64 (1,69) μg/mL. With the Spearman Test was showed that there was a relationship between UEI and TGR at 5% level of significance (p=0,001).There was no relationship between Urine Thiocyanate Levels and TGR at 5% level of significance (p=0,491). The R2 value was 0,022 using Regression Test for EIU and TGR.Conclusion : The UEI levels have a significantly relationship with TGRKeywords : IDD, TGR, UEI levels, Urine Thiocyanate Excretion levels

HUBUNGAN ANTARA BESI SERUM DAN SATURASI TRANSFERIN DENGAN KADAR HEPSIDIN PADA CARRIER TALASEMIA- β

MEDIA MEDIKA MUDA 2012:MMM VOLUME 1 NUMBER 1 YEAR 2012
Publisher : MEDIA MEDIKA MUDA

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Latar belakang: Penyakit genetik anemia hemolitik seperti talasemia terutama carrier talasemia umumnya jarang diketahui oleh masyarakat awam, namun tingkat prevalensinya cukup tinggi dalam penyebab mortalitas dan morbiditas. Hal tersebut terjadi dikarenakan penggunaan suplementasi besi yang berlebih sehingga terjadi iron overload. Iron load diatur oleh hepsidin. Hepsidin mempunyai peran dalam regulasi homeostasis besi dan aktivitas eritropoeisisdalam tubuh manusia. Oleh sebab itu penelitian ini bertujuan untuk mengetahui hubungan kadar hepsidin dengan besi serum dan saturasi transferin pada carrier talasemia-β.Metode: Desain penelitian ini adalah cross sectional dengan pendekatan observasional analitik. Pengambilan sampel dengan cara consecutive sampling dari data primer yang dilakukan di laboratorium Patologi Klinik RSUP Dr.Kariadi dan PMI kota Semarang. 26 responden dilakukan pengambilan darah sebanyak 6 ml. Kemudian dilakukan pengukuran kadar hepsidin menggunakan ELISA reader berdasarkan gelombang spektrofotometri, sedangkan untuk pengukuran status besi dan saturasi transferin menggunakan alat automatic analyzer. Analisa data diolah menggunakan program komputer dengan taraf signifikansi diterima bila p> 0,05 dan perhitungan data numerik.Hasil: Sampel penelitian melibatkan 26 responden. Hasil uji Spearman menunjukkan tidak terdapat hubungan yang bermakna antara besi serum dengan kadar hepsidin (p= 0,071, r= 0,077), dan saturasi transferin dengan kadar hepsidin (p= 0,211, r= 0,373) pada carrier talasemia-β.Simpulan: Kadar hepsidin tidak memiliki hubungan yang bermakna dengan besi serum dan saturasi transferin pada carrier talasemia-β.Kata kunci: Carrier talaesemia-β, kadar hepsidin, besi serum dan saturasi transferin

Pengaruh suplementasi β-carotene terhadap kadar glukosa darah dan kadar malondialdehida pada tikus sprague dawley yang diinduksi Streptozotocin

JURNAL GIZI INDONESIA Vol 2, No 2 (2014)
Publisher : JURNAL GIZI INDONESIA

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Background : Hyperglycemia on diabetes mellitus can cause increasing of free radicals production. Free radicals caused lipid peroxidation reaction by forming malondialdehyde (MDA). β-carotene has antioxidant activity may inhibit the formation of ROS.Objective : To prove the effect of multilevel doses β-carotene 1 mg/kg BW, 20 mg/kg BW and 20 mg/kg BW on alternate days within 30 days orally supplementation on blood glucose level and MDA level on Sprague Dawley male rats induced by streptozotocin (STZ). Methods : Thirty rats were randomly divided into 5 groups: X1=Negative control/diabetic, X2 (STZ 40 mg/kg BW + BC 1 mg/kg BW), X3 (STZ 40 mg/kg BW + BC 10mg/kg BW), X4 (STZ 40 mg/kg BW + BC 20 mg/kg BW), X5 (technic control/non diabetic). β-Carotene supplementation was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP and MDA level by ELISA with TBARS methods. Data was analized using paired t-test, wilcoxon, one way anova and post hoc bonferroni. Results : there was a significant difference of blood glucose level (p = 0,0001) and MDA level (p = 0,0001) after suplementation β-carotene on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most effective and efficient dose to lowering blood glucose, while 20 mg/kg BW to lowering MDA level. Conclusion : The multilevel doses β-carotene (1 mg/kg BW, 10 mg/kg BW and 20 mg/kg BW) on alternate days within 30 days orally supplementation can decrease blood glucose and MDA level. β-carotene 10 mg/kg BW is the most effecetive and efficient to decrease blood glucose and β-carotene 20 mg/kg BW to decrease MDA level.

Efek suplementasi β-carotene terhadap kolesterol total, trigliserida dan malondialdehid pada tikus sprague dawley yang diabet

JURNAL GIZI INDONESIA Vol 2, No 2 (2014)
Publisher : JURNAL GIZI INDONESIA

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Background : diabetes mellitus (DM) is associated with increased total cholesterol and triglyceride, also well characterized by increased malondialdehyde production. β-carotene has antioxidant activity, glycemic and lipid control. Objective : to analyze the effect of β-carotene on total cholesterol, triglyceride and MDA on diabetic Rattus norvegicus sprague dawley. Methods : thirty rats were randomly divided into 5 groups : 1 (STZ), 2 (STZ+β-carotene 1 mg/kg BW), 3 (STZ+β-carotene 10 mg/kg BW), 4 (STZ+β-carotene 20 mg/kg BW), 5 (normal). Streptozotocin induced intraperitoneal 40 mg/kg BW. β-carotene was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP, total cholesterol by CHOD-PAP, triglyceride by GPO and MDA by ELISA with TBARS methods. Hypothesis test used one way anova then followed by post hoc bonferroni to analyze the efficient dose effect. Results : there was a significant difference of total cholesterol (p=0.002) after β-carotene 10 mg/kg BW supplementation on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most efficient dose to lowering total cholesterol. There were significant differences of triglyceride (p=0.0001) and MDA (p=0.0001) after β-carotene 1, mg/kg BW, 10 mg/kg BW and 20 mg/kg BW supplementation on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most efficient dose to lowering triglyceride, while 20 mg/kg BW to lowering MDA. Conclusion : β-carotene 10 mg/kg BW is the most efficient dose to lowering total cholesterol and triglyceride. β-carotene 20 mg/kg BW is the most efficient dose to lowering MDA.

Pengaruh suplementasi vitamin e (α-tokoferol) terhadap kadar gamma glutamil transferase (ggt) dan kadar nitric oxide (no) pada tikus (Studi pada tikus rattus novergicus strain wistar jantan terpapar inhalasi uap benzene)

JURNAL GIZI INDONESIA Vol 3, No 2 (2015)
Publisher : JURNAL GIZI INDONESIA

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Latar Belakang : Paparan benzene dapat menyebabkan stres oksidatif, diantaranya ditandai dengan peningkatan kadar GGT dan kadar NO. Vitamin E dapat mengurangi dan mencegah produksi radikal bebas. Reaktivitas hidrogen fenolik pada kelompok hidroksil α-tokoferol akan menstabilkan elektron tidak berpasangan pada radikal bebas. Tujuan : Membuktikan pengaruh suplementasi vitamin E terhadap kadar GGT dan kadar NO pada tikus jantan terpapar uap benzene. Metode : Penelitian ini adalah penelitian eksperimen pada binatang coba dengan rancangan post test only controlled group design. Sampel 35 ekor tikus strain wistar jantan dibagi secara random dalam enam kelompok yaitu kontrol negatif, kontrol positif dan empat kelompok perlakuan suplementasi vitamin E dosis bertingkat (X1 = 1,8 IU;  X2 = 3,6 IU; X3 = 7,2 IU; dan X4 = 14,4 IU per hari untuk setiap 200 gram berat badan tikus). Setiap kelompok kecuali kontrol negatif dipapar 300 ppm uap benzene selama 2 minggu (6 hari/minggu, 6 jam/hari). Pengukuran kadar GGT menggunakan metoda Kinetik Colorimetric dan kadar NO menggunakan metoda Colorimetric Gries dilakukan pada setiap kelompok. Hasil : Suplementasi vitamin E berpengaruh menurunkan  kadar GGT (p=0,0001). Pengaruh paparan benzene terhadap peningkatan kadar GGT dapat dicegah dengan baik pada dosis suplementasi vitamin E 14,4 IU/200gr BB tikus/hari. Suplementasi vitamin E tidak berpengaruh terhadap kadar NO. Simpulan : Ada pengaruh suplementasi vitamin E terhadap penurunan kadar GGT dan  tidak ada pengaruh suplementasi vitamin E terhadap penurunan kadar NO.

PERBEDAAN NEUTROPHIL-LYMPHOCYTE RATIO PADA SUBJEK BUKAN PEROKOK, PEROKOK RINGAN DAN PEROKOK SEDANG-BERAT

JURNAL KEDOKTERAN DIPONEGORO Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Jurusan Kedokteran Umum, Fakultas Kedokteran, Universitas Diponegoro

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Latar Belakang : Merokok merupakan problem kesehatan serius yang menyebabkan angka kesakitan dan kematian yang tinggi. Paparan asap rokok yang berlangsung lama dapat memicu inflamasi pada saluran nafas dan parenkim paru perokok serta mempengaruhi jumlah dan hitung jenis leukosit. White Blood Cell Count (WBC) menggambarkan status inflamasi. Neutrofil dan limfosit merupakan bagian terbesar dari leukosit sehingga mampu menggambarkan sebagian besar respon imun tubuh. Neutrophil-Lymphocyte Ratio (NLR) dapat menggambarkan status inflamasi pada perokok.Tujuan : Membuktikan adanya perbedaan NLR antara subjek bukan perokok, perokok ringan dan perokok sedang-berat.Metode : Penelitian deskriptif analitik dengan pendekatan belah lintang dengan subjek penelitian sebanyak 36 orang yang dibagi menjadi tiga kelompok menurut klasifikasi perokok Sitepoe, yaitu kelompok subjek bukan perokok, perokok ringan dan perokok sedang berat. NLR didapat dengan membandingkan jumlah neutrofil absolut dengan jumlah limfosit absolut. Uji statistik menggunakan uji one way Anova dan uji Post Hoc Bonferroni.Hasil : Rerata NLR pada kelompok subjek bukan perokok sebesar 2,42 ± 0,51, kelompok subjek perokok ringan sebesar 3,01 ± 1,29 dan kelompok subjek perokok sedang-berat sebesar 2,02 ± 0,63. Uji Post Hoc menunjukkan ada perbedaan antara nilai NLR kelompok subjek perokok ringan dan perokok sedang berat (p=0,030) dan tidak ada perbedaan antara nilai NLR kelompok subjek bukan perokok dengan kedua kelompok lainnya (p=0,348; p=0,821).Kesimpulan : Terdapat perbedaan antara nilai NLR subjek perokok ringan dan perokok sedang-berat, namun tidak ada perbedaan antara nilai NLR antara subjek bukan perokok dengan perokok ringan maupun perokok sedang-berat.

Vitamin E effect on osmotic fragility in β thalassemia major

Paediatrica Indonesiana Vol 54, No 5 (2014): September 2014
Publisher : Indonesian Pediatric Society

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Background Blood transfusion remains the main therapy for anemia in β thalassemia major patients. However, frequent transfusions can cause oxidative stress in response to iron overload. Vitamin E is considered to be the best lipid-soluble exogenous antioxidant in humans. It can protect phospholipid membrane from peroxidarion. Erythrocyte osmotic fragility is a useful test to assess for the improvement of red blood cells in thalassemia patients after vitamin E supplementation.Objective To investigate the effect of vitamin E for improving erythrocyte osmotic fragility in β thalassemia major and for decreasing the need for frequent transfusions.Methods T his was a double blind placebo controlled randomized clinical trial on children aged 2-14 years with thalassemia major who received frequent blood transfusions. Fifty subjects were divided into 2 groups: group I with vitamin E supplementation and group II with placebo, as a control group, for a period of 1 month. Pre- and post-treatment data on erythrocyte osmotic fragility and hemoglobin level were analyzed with non-paired T-test.Results Improved erythrocyte osmotic fragility was found: in group I, pre-treatment 31.59 (SD 6.342)% to post-treatment 38.08 (SD 7.165)%, compared to the control group pre-treatment 34.40 (SD 6.985)% to post-treatment 29.26 (SD 9.011)% (P=0.0001). Comparison of the mean delta Hb level in group  I was 0.94 (SD 0.605) gr% and that of group II was - 0.23 (SD 1.199) gr% (P=0.0001).Conclusion Vitamin E supplementation improves erythrocyte fragility and Hb level in β thalassemia major pediatric patients.

Pengaruh pemberian minuman lidah buaya terhadap kadar antioksidan total dan persentase lemak tubuh pada sindrom metabolik

Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 7, No 1 (2018)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

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Abstract

Background: Metabolic syndrome is related to glucose metabolism disturbance (hyperglycemia), lipid (dyslipidemia), high blood pressure, and central obesity. Metabolic syndrome implicates to heart attack, diabetes mellitus, hypertension, and death. Aloe vera, well known rich of polyphenol and vitamin, has a pharmacological effect to improve insulin resistance, chronic inflammation, and oxidative stress.Objective: To prove the effect of Aloe vera-based drink toward total antioxidant concentration improvement and body fat percentage reduction in metabolic syndrome subjects.Methods: Thus study was pre-post randomized true experimental study with control group design. The subjects were divided by 2 groups, treatment group (n=19) and control group (n=19). Treatment group was given 165 g/d Aloe vera-based drink for 30 days. Both of groups were given nutrition education about metabolic syndrome management. Total antioxidant concentration and body fat percentage were assessed pre-post-test by ABTS and BIA respectively, while food intake and activity were assessed by 24-h recall and GPAQ respectively. Independent t-test and Mann Whitney test were used to compare before and after treatment between control and treatment group.Results: Total antioxidant concentration significantly improved (p=0.00) in treatment group from 1.2 to 2.0 mmol/L, while total antioxidant concentration decreased from 1.4 to 1.3 mmol/L (p=0.074) in this control group. Moreover, there was significant difference of total antioxidant between treatment and control groups in the end of study (p=0.00). Furthermore, body fat percentage was reduced significantly from 32.8 to 32,4 mmol/L (p=0.005) in treatment group, while the reduction of body fat percentage in control group was not significant from 33.1 to 33,4 mmol/L (p=0.100). There was no difference of body fat percentage between two groups after intervention (p=0.358).Conclusion: Aloe vera-based drink improved total antioxidant concentration in metabolic syndrome subjects.

Baseline and Post-exercise High-Sensitivity C-Reactive Protein Levels in Endurance Cyclists: The Indonesian North Coast and Tour de Borobudur 2017 Study

The Indonesian Biomedical Journal Vol 11, No 1 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

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BACKGROUND: Inflammation plays an important role in the atherosclerotic process. High-sensitivity C-reactive-protein (hs-CRP) is commonly used as inflammatory biomarker. It is well known that regular physical activity lowers hs-CRP levels, while prolonged exercise induces hs-CRP elevations. However, the relationship of training and exercise characteristics with hs-CRP levels remains not well elucidated. We evaluated baseline and post-exercise hs-CRP levels and its association with training and exercise characteristics.METHODS: Eighty-eight male endurance cyclists were involved. Demographic data, health condition and training characteristics were collected. Baseline and postexercise blood-samples were collected to determine hsCRP concentrations. A hs-CRP cut-off point of 3 mg/L was used. Blood-cell count and biochemical parameters were measured at baseline. Heart rate (HR) was measured during exercise.RESULTS: Cyclists performed 7.3 hours (interquartilerange (IQR) = 5.4-7.5) of endurance exercise at intensity of 81.8 % (IQR = 74.9-85.8). Cyclists with baseline hsCRP ≥ 3 mg/L reported higher body mass, body mass index (BMI), waist-circumference and total-cholesterol. An increase in hs-CRP was following endurance exercise. Cyclists with any elevation of hs-CRP reported a higher BMI, HR during exercise and exercise intensity. Binary logistic regression analysis showed BMI (OR = 1.24, 95% CI = 1.04-1.48) and cycling distance (OR = 0.22, 95% CI = 0.06-0.76) were associated with post-exercise hs-CRP elevations.CONCLUSION: Body mass, BMI, waist-circumference, total- and HDL-cholesterol are associated with baseline hsCRP, whereas BMI and cycling distance were associated with hs-CRP elevations. These findings suggest that anthropometry parameters and lipid levels attributed to baseline hs-CRP, while anthropometry parameters and cycling intensity attributed to post-exercise hs-CRP elevations.KEYWORDS: C-reactive-protein, exercise, endurancecycling, inflammation, acute-phase-response 

PENGARUH PEMBERIAN JAMUR KUPING HITAM (AURICULARIA POLYTRICHA) TERHADAP KADAR TRIGLISERID SERUM TIKUS WISTAR YANG DIINDUKSI MINYAK JELANTAH

JURNAL KEDOKTERAN DIPONEGORO Vol 6, No 2 (2017): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Program Studi Kedokteran, Fakultas Kedokteran, Universitas Diponegoro

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Latar belakang : Pada era modern seperti sekarang ini banyak sekali orang-orang yang lebih memilih untuk makan-makanan cepat saji. Namun biasanya minyak yang digunakan untuk menggoreng makanan tersebut adalah minyak jelantah. Padahal minyak jelantah itu sendiri memiliki lemak trans yang begitu tinggi, sehingga menyebabkan naiknya kadar kolesterol salah satunya trigliserid. Hal itu dapat menyebabkan meningkatnya kejadian suatu penyakit seperti aterosklerosis. Jamur kuping hitam diketahui memiliki kandungan vitamin, polisakarida, dan zat-zat lain yang dapat menurunkan kadar trigliseridaTujuan : Mengetahui efek pemberian jamur kuping hitam terhadap penurunan kadar trigliserid pada tikus wistar jantan yang diinduksi minyak jelantahMetode : Penelitian ini berjenis true eksperimental dengan post-test only with control group design menggunakan 24 tikus wistar sebagai objek percobaan. Kemudian dibagi menjadi 4 kelompok. Kelompok K1 diberi diet standar dan aquades 2ml/200 gramBB. Kelompok K2 diberi diet standar, aquades 2ml/200 gramBB dan minyak jelantah sebanyak 3ml/200 gramBB. Kelompok K3 diberi diet standar dan air rebusan jamur kuping hitam sebanyak 2ml/200 gramBB. Dan kelompok  K4 diberi diet standar, minyak jelantah 3ml/200 gramBB, dan air rebusan jamur kuping hitam 2ml/200 gramBB. Data dianalisis dengan uji Saphiro-Wilk, Levene test, oneway ANOVA, dan Post Hoc testHasil : Diperoleh hasil yang signifikan dilihat dari rerata kelompok dengan  kadar trigliserid dimulai dari terendah yaitu kelompok K3 (81,56 mg/dL),kemudian kelompok K1 (88,88 mg/dL),kemudian kelompok K4 (98,99 mg/dL),dan terakhir kemudian kelompok K2 (129,42 mg/dL)Simpulan : Pemberian air rebusan jamur kuping hitam dapat menurunkan kadar trigliserid