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Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line

Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of  C.  roseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 μg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 μg/mL induced apoptosis for 22.235%.  Meanwhile doxorubicin at concentration of 10  μg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by using  in vitro assay: inhibition of  2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging  activity. Antioxidant activity of  C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls.  The results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 μg/mL, 19.200 μg/mL  and  94.178  μg/mL, respectively. We suggest that  C. roseus extract had a potential anticancer activity by inducing apoptosis.

Free Radical Scavenging Activity of Ethanolic Leaves Extract and Its Different Solvent Fractions of Piper betle L. In Vitro

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Various diseases including cancer and cardiovascular diseasesare induced by free radicals via lipid peroxidation, protein peroxidation, DNA damage, and cellular degeneration in thecells.Piper betle L.,commonly called betel,belongs to thePiperaceae family.It originated from South and South Eas tAsia,and has been used as a traditional medicine by people theredue to its medicinal properties.In the present study, free radicalscavenging activity of ethanolic leaves extract of Piper betle L.together with its different solvent fractions(hexane,ethylacetate,butanol,and water)were evaluated by DPPH freeradical scavenging assay.The ethanolic extract and the ethylacetate fraction had shown very significant DPPH (1,1)diphenyl)2)picryl)hydrazyl) radical scavenging activitycompared to the other fractions.In DPPH free radical scavengingassay,the IC50 value of the ethanolic extract and the ethyl acetate fraction were found to be 17.431g/mL and 11.53 1g/mL, respectively. The free radical scavenging activity was assumed due to the total phenolic content.The total phenoliccontent was found to be highest in the ethanol extract(277.68µgEGCG/mg)and the ethyl acetate fraction(559.38µgEGCG/mg).The results concluded that the ethanolic leaves extract of Piper  betle L. and its ethyl acetate fraction have a consider ably freeradical scavenging activity.