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Uji Fitokimia dan Potensi Antioksidan Ekstrak Etanol Kayu Secang (Caesalpinia sappan L.)

Jurnal Kedokteran Maranatha Vol 11, No 1 (2011)
Publisher : Universitas Kristen Maranatha

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Abstract

Penyakit degeneratif dan penyakit kronis sering disebabkan oleh stres oksidatif yang dipicu oleh radikal bebas. Untuk mencegah terjadinya stres oksidatif diperlukan antioksidan. Kayu secang (Caesalpinia sappan L.) sering dikonsumsi sebagai minuman seduhan karena dipercaya dapat menyembuhkan berbagai jenis penyakit. Penelitian ini bertujuan menguji kadar senyawa fitokimia dan kadar total fenol ekstrak etanol dalam kayu secang dengan menggunakan standar epigalokatekin (EGC) dan epikatekin galat (ECG), serta menguji aktivitas antioksidan dengan menggunakan metode pemerangkapan radikal bebas 1,1-difenil-2-pikrilhidrazil (DPPH). Penelitian ini membandingkan aktivitas antioksidan dalam ekstrak kayu secang dengan EGC dan ECG dalam 6 level konsentrasi (100; 50; 25; 12,5; 6,25; 3,125 μg/ml). Data dianalisis dengan menggunakan analisis rancangan acak lengkap dan dilanjutkan dengan uji jarak berganda Duncan. Uji fitokimia secara kualitatif serta uji total fenol menggunakan standar ECG dan EGC. Hasil penelitian menunjukkan ekstrak kayu secang mengandung senyawa terpenoid, fenol sangat tinggi, tetapi tidak mengandung steroid dan tanin. Ekstrak kayu secang mengandung kadar total fenol ekivalen EGC adalah 849,11 μg/mg dan ekivalen ECG 825,11 μg/mg. Ekstrak kayu secang memiliki aktivitas antioksidan tinggi yaitu 80,46-89,13%

Free Radicals Scavenger Potency of Betel Leaves (Piper betel L.) Extract and Various Fractions

Jurnal Kedokteran Maranatha Vol 10, No 1 (2010)
Publisher : Universitas Kristen Maranatha

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Abstract

The imbalance between free radicals and antioxidants in the biology system can cause various diseases. Betel leaves (Piper betel L.), parts of a medicinal plant, are popularly used as a herbal remedy for diseases, but the scientific basis especially of their antioxidant properties remains unknown. To evaluate free radicals scavenger activity of ethanol extract and various fractions (hexane, ethyl acetate, butanol and water fraction), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and hydroperoxide (H2O2) scavenging activity were determined. To determine the DPPH and H2O2 scavenging activity, betel leaves extract and fractions were prepared in 10 concentrations. The result of this research showed that betel leaves extract and fractions had higher H2O2 scavenger and lower DPPH scavenger activities than gallic acid. The highest DPPH scavenging activity with Inhibitory Concentration (IC50) was found in gallic acid 0.732 μg/mL, ethyl acetate fraction 3.156 μg/mL, and ethanol extract 5.489 μg/mL. The highest H2O2 scavenging activity with IC50 was found in butanol fraction 0.223 μg/mL, gallic acid 0.597 μg/mL, and ethyl acetate fraction 0.783 μg/mL. In conclusion, betel leaves extract and fractions were potential free radicals scavenger; they could be potential candidates to inhibit oxidative stress. Gallic acid and ethyl acetate fraction were the highest free radicals scavenger both in DPPH and H2O2 free radical scavenging activity.Keywords: free radical, betel leaves, DPPH, H2O2, antioxidant

Hubungan antara Kadar Karbon Monoksida (CO) Udara dan Tingkat Kewaspadaan Petugas Parkir di Tiga Jenis Tempat Parkir

Jurnal Kedokteran Maranatha Vol 10, No 1 (2010)
Publisher : Universitas Kristen Maranatha

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Abstract

Tingkat polusi udara di Indonesia semakin meningkat, salah satu sebabnya adalah bertambahnya jumlah kendaraan bermotor yang meningkatkan jumlah emisi gas buang. Salah satu senyawa di dalam emisi gas buang adalah karbon monoksida (CO). Penelitian ini bertujuan untuk mengetahui perbedaan kadar CO udara di 3 jenis tempat parkir dan pengaruhnya terhadap tingkat kewaspadaan petugas parkir. Desain penelitian ini adalah observasional analitik yang bersifat komparatif. Data yang diukur adalah kadar CO udara di tempat parkir terbuka (TPB), semi terbuka (TPSB), dan tertutup (TPT). Subjek penelitian adalah 18 orang petugas parkir yang bekerja di 3 jenis tempat parkir. Kadar CO udara diukur menggunakan alat CO–CO2 meter SIBATA, sedangkan tingkat kewaspadaan diukur dengan Jhonson Pascal Test. Data dianalisis menggunakan ANOVA satu arah, uji lanjut Duncan’s Post Hoc Test dengan α = 0,05 dan uji regresi korelasi linier. Hasil pengukuran menunjukkan bahwa kadar CO udara di TPT (13,86 ppm) lebih tinggi daripada di TPB (1,96 ppm) dan TPSB (2,32 ppm) dan tingkat kewaspadaan petugas parkir tidak dipengaruhi oleh kadar CO udara baik di TPB (r = 0,35), TPSB (r = 0,001), maupun di TPT (r = 0,044). Simpulannya, terdapat perbedaan kadar CO udara di 3 jenis tempat parkir, akan tetapi tidak ada hubungan antara kadar CO udara dan tingkat kewaspadaan petugas parkir.Kata kunci: CO, tingkat kewaspadaan, tempat parkir terbuka, tempat parkir semi terbuka, tempat parkir tertutup.

Potensi Antioksidan sebagai Antidiabetes

Jurnal Kedokteran Maranatha Vol 7, No 2 (2008)
Publisher : Universitas Kristen Maranatha

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Abstract

Diabetes mellitus (DM) is a syndrome  that is marked by elevated blood sugar level. DM is  categorized into two types:  type 1 DM is called insulin-dependent diabetes mellitus (IDDM) and type 2 DM is  called non-insulin-dependent diabetes mellitus (NIDDM). Glucose can be oxidized before or after bound with protein (glycated protein), producing Reactive Oxygen Species (ROS). Diabetic patients indicate lipid peroxide and Thiobarbituric Acid Reactive Subtances  (TBARS) plasma levels that are higher than normal individuals. The combination of glycation and glucose oxidation forms AGEs (Advanced Glycogen End-products). Glycated protein and AGEs modified protein can cause oxidative stress by  releasing  O2*- ,H2O2 and  carbonyl  toxic can damage protein. Many kinds of plants contain  active compounds that can inhibit carbohydrate hydrolysis and glucose absorption, regenerate β-cells so as to increase the release of insulin, inhibit aldose reductase and control the glucose level. Synthetic as well as natural antioxidant compounds from various plants can control glucose level and inhibit diabetic complications. Polyphenolic active compounds in  plants have antioxidant and hypoglycemic activities. To reduce the amount of type 1 DM patients it is necessary to change the life style, among others by consuming  enough vegetables and fruits as antioxidant sources.

Potensi Tumbuhan Erythrina (Leguminosae) sebagai Antifertilitas

Jurnal Kedokteran Maranatha Vol 7, No 2 (2008)
Publisher : Universitas Kristen Maranatha

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Abstract

Erythrina plants (Leguminosae) were used as traditional antifertility agents. In the continuing research for novel plant with antifertility capacity from Indonesian plants, the methanol extract of Erythrina plants showed significant antifertility activity in an in vitro assay of spermatozoa of white rat (R. norvegicus). The methanol extract was separated using bioassay-guide n-hexane, ethylacetate, and n-buthanol fractionation. The ethylacetate fraction was tested in vitro on spermatozoa of white rat (R. norvegicus) at a concentration of 0.25 x 10-3 μg/μL. It showed a decrease of their motilities and viabilities, as well as an increasing abnormality of the spermatozoa.

Penapisan Aktivitas Superoksida Dismutase pada Berbagai Tanaman

Jurnal Kedokteran Maranatha Vol 5, No 1 (2005)
Publisher : Universitas Kristen Maranatha

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Abstract

Free radicals can be damaged on cell integration, because free radicals very reactive to cell component either structur component or functional component. To scavenge and inhibit chain reaction of free radicals were required exogen antioxidant. Natural antioxidant from plant was more interested because its more safe than synthetic antioxidant. Plant in the world such as spices, vegetables, fruits, medicine plants much contain antioxidant. Antioxidant activity to dismutate superoxide radical (O2-) from various plant was expected capable to help know potential natural antioxidant resources. This research was found out that salam leaf (Eugenia polyanta Wight), sappan wood (Caesalpinia sappan L.), seed and fruit guava have (Psidium guajava L.) superoxide dismutase (SOD) activity 100%.