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The Potency of Xanthones as Antioxidant and Antimalarial, and their Synergism with Artemisinin in Vitro

Journal of the Indonesian Medical Association Vol. 63 No. 3 March 2013
Publisher : Journal of the Indonesian Medical Association

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Abstract

Introduction: Artemisinin in artemisinin based combination therapy (ACT) was used to overcome the resistance caused by free radical overproduction. Xanthone as antioxidant can also inhibit in vitro heme polymerization, therefore it is predicted has anti-malaria activity. The aim of this study is to evaluate the potency of alpha mangostin, gamma mangostin, garcinone C, and garcinone D as antioxidant by determination of DPPH scavenging activity, falciparum antimalarial, and their synergism with artemisinin as antimalarial falciparum in vitro.Method: Diphenylpicrylhydrazyl (DPPH) scavenging activity was examined according to Unlu,et al.’s technique and the IC50 was determined by correlation regression analysis. Antimalarialactivity of each xanthone and its combination with artemisinin was evaluated in P. falciparumstrain 3D7 culture according to Budimulja, et al. Their IC50 was calculated by probit analysis and FIC50 was counted according to the formula.Results: All of these xanthones had IC50 of DPPH scavenging activity <200 μM. Alpha mangostin, gamma mangostin, and garcinone C had anti-malaria activity with IC50<1 μg/ml, but garcinone D had IC50 1-10 μg/ml. FIC50 of all xanthone and artemisinin combinations were <1.Conclusions: All of xanthones have antioxidant potency by determination of DPPH scavengingactivity, antimalarial potency and work synergisticaly with artemisinin as falciparum antimalarialin vitro. J Indon Med Assoc. 2013;63:95-9.Keywords: xanthone, DPPH, falciparum antimalarial, artemisinin, synergism