Winarso Dradjad Widodo
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Pollen Viability and Pollen Tube Growth of IPB’s Papaya Suketi, Ketty; Tuharea, Cenra Intan Hartuti; Widodo, Winarso Dradjad; Poerwanto, Roedhy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 1 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The purpose of the research  was to examine the pollen germination process and growth rate of pollen tubes of papaya. Pollen tube growth of nine genotypes of papaya (IPB 1, IPB 2, IPB 3, IPB 4,  IPB 5, IPB 7, IPB 8,  IPB  9,  and  IPB 10) was investigated in this experiment in order to study their pollen germination rate and pollen viability. The fresh pollen were excised from the flowers of papaya grown at Tajur Field Station of  Research Center for Tropical Fruit, Bogor Agricultural University (IPB), Bogor. The extracted pollen was cultured aseptically on the Brewbaker and Kwack medium (pH 7.3) at ambient  temperature of 26-28 °C. Pollen germination and pollen tube growth was observed under optical microscope with 100 and 400 magnification. Papaya pollen viability was not associated with size-based categories of papaya fruits. IPB 4 had the longest pollen tube at first 30 minutes after germination  (115.5 µm), whereas IPB 10 had the shortest (99.5 µm).  The distance  from  stigma  to   ovary  in  hermaphrodite  flowers  varies with genotypes, ranging from 7.38 to 13.44 mm.  Average length of pollen tube within four hours of germination for small papaya fruit category (IPB 1, IPB 3, and IPB 4) was 1,030.67 ± 19.14 #m, while the distance between stigma and ovary was short (14.85 ± 2.19 mm) so that the expected of  fertilization process occurred sooner.  At the end of the experiment (four hours after germination), IPB 1 genotype  had the longest pollen tube (1,052 µm) while IPB 9 genotype (913 µm) had the shortest  pollen tube.  Genotype with the highest percentage of germination  at the end experiment was IPB 2 (65.65%), whereas the lowest was IPB 7 (42.56%).   Keywords: Carica papaya, germination rate, pollen germination
Aktivitas Hormon Endogen dalam Buah Anggur Muscat ofAlexandria Muda Tanpa Biji Hasill Induksi Antibiotika Widodo, Winarso Dradjad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 30, No 3 (2002): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

The experiment was conducted to determine the activity of endogenous hormones in young seedless berry of Muscat of Alexandria (MOA) grapes induced by streptomycin (SM) and spectinomycin (SE). Young berries dipped in 200 ppm M or 200 ppm SE at 3 days before full-bloom and untreated berries were analyzed for the activity of . endogenous auxin, cytokinin. gibberellin and abscisic acid (ABA). The experiment was conducted at the Laboratory of ! Pomology, Faculty of Agriculture, Okayama University, Japan, from February 1999 to August 1999. Both SM and SE reatments did not affect ABA activities of young berries. Cytokinin activity were slightly decreased by SM and SE treatments at 6 days after treatment (DAT) and became more severe at J 0 and J 3 DAT The response of berry auxin activity to antibiotic treatment was similar but stronger than cytokinins. Antibiotic treatments significantly decreased the activity ofGA at 6 and 10 DAT   1 Keywords,´ Antibiotics, Endogenous hormones, Spectinomycin, Streptomycin