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Profil Kadar Kortisol dan Seng pada Kambing Peranakan Etawah Saat Melahirkan yang Diberi Tambahan Seng dalam Pakannya Derthi Widhyari, Sus; Widodo, Setyo; Teguh Wibawan, I Wayan; Sutama, I Ketut; Esfandiari, Anita
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Jurnal Veteriner

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The objective at this experiment was to study the role of zinc supplementation (Zn) in minimizingstress at parturition. Thirty etawah cross breed age 3-6 years with average body weight 30-50 kg were usedin this study and divided into three groups ; i) animal were given grass + feed concentrate + ZnSO4 40 mg/kg dry matter ( control group) ; (ii) grass + feed concentrate + ZnSO4 60 mg/kg dry matter (treatment 1), and(iii) grass + feed concentrate + ZnSO4 80 mg/kg dry matter (treatment 2), respectively drinking water weregiven ad libitum. To monitor zinc and cortisone level, blood samples were withdrawn from each animalevery two weeks starting at age of pregnancy 3 months up to two months post parturition, in addition, tomonitor the cortisone level blood samples were also collected at 1,2,3 and 7 days post parturition. The Znand cortisone serum were analyzed using atomic absorbent spectro photometric (AAS) andradioimmunoassay (RIA), respectively the result showed that generally pregnancy at the beginning andget serum level at cortisone was relatively similar between the three animal groups and there was atendency to increase and reached peak at parts then gradually decrease until the and of the observation. Atparturition the lowest serum level at cortisone (19.00±18.72 mg/ml) was observed in animals receiving the60/ dry matter zinc supplement compared to animal receiving 80 mg/ dry matter zinc ( 52.65 ± 30.83 mg/ml) and control animal ( 75.92 ± 42.88 mg/ml). in addition serum level at Zn was significantly higher inanimal receiving 60 mg and 80 mg Zn in their diets compared to control animals. The best profiles wereseen in animal given 60 mg/kg dry matter Zn supplement. In conclusion, the addition of Zn in the diets hadsignificant effect in minimizing stress as pictured by the low serum level at cortisone at parturition.
Anestesi Infus Gravimetrik Ketamin dan Propofol pada Anjing (THE GRAVIMETRIC INFUSION ANAESTHESIA WITH KETAMINE AND PROPOFOL IN DOGS) Sudisma, I Gusti Ngurah; Widodo, Setyo; Sajuthi, Dondin; Soehartono, Harry
Jurnal Veteriner Vol 13, No 2 (2012)
Publisher : Jurnal Veteriner

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This study aim was to evaluate quality of anaesthesia by using gravimetric infusion anaesthesia withketamine and propofol in dogs. The quality of anaesthesia, duration of actions, and the physiological responsseof anaesthesia were evaluated in twenty domestic dogs. Anaesthesia was induced intramuscularly withatropine (0.03 mg/kg)-xylazine (2 mg/kg) (AX), intravenously ketamine-propofol (KP) (4 mg/kg), andmaintained with continuous intravenous infusion with pre-mixed propofol (P) and normal saline containing2 mg/ml of propofol and 2 mg/ml of ketamine (K). Domestic stray dogs were randomly divided into fivegroups. Groups AXKP-K2P2, AXKP-K4P4, and AXKP-K6P6 were treated with ketamine-propofol the dose0.2 mg/kg/minute, 0.4 and 0.6 mg/kg/minute respectively, while group AXKP-P4 was given propofol 0.4 mg/kg/minute and group AXKP-I was given isoflurane 1-2%. Heart rate (HR), respiratory rate (RR),electrocardiogram (ECG), blood oxygen saturation (SpO2), end tidal CO2 (ET CO2), and capillary refill time(CRT) were measured. No significant difference (P>0.05) found between the groups in anaesthetion times.All groups showed rapid and smooth inductions, prolonged surgical stage, and rapid recovery. Groups AXKPK2P2and AXKP-K4P4 showed minimal physiological effect on the dogs. The HR, RR, ET CO2, SpO2, CRT,and ECG wave were stabl. Combination of AXKP-K6P6 induced SpO2 depression, increased and instabilityof HR, RR and ET CO2. Groups AXKP-P4 showed decreased of HR and respiratory depression. All anaestheticcombinations showed no significant influence (P>0.05) on the electricity of the dog’s heart. The combinationof ketamine-propofol at dose 0.2 and 0.4 mg/kg/minute were found to be better as an application formaintaining anaesthesia by gravimetric continuous intravenous infusion. The method is a suitablealternative for inhalation anaesthesia in dogs.
Gambaran kristal urin pada hewan model nefrolitiasis hasil induksi etilen glikol Purwono, Rini Madyastuti; Wientarsih, Ietje; Widodo, Setyo; Purwaningsih, Erni H; Harlina, Eva
ARSHI Veterinary Letters Vol 3, No 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

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Tingginya tingkat keterulangan kasus urolitiasis baik pada hewan maupun manusia, menuntut penelitian dalam mencari solusi menurukan angka keterulangan. Penelitian urolitiasis membutuhkan hewan model urolitiasis yang sesuai dengan kondisi sebenarnya. Salah satu metode yang digunakan adalah menciptakan kondisi hiperoksaluria. Penggunaan induksi etilen glikol menjadi metode yang paling umum digunakan. Induksi ini menghasilkan kristal kalsium oksalat monohidrat. 
Using homeopathica drugs combination at peripartal phase in preventing bovine subclinical mastitis Sanjaya, Agatha W.; Wibawan, I W.T.; Sudarwanto, Mirnawati; Widodo, Setyo; Enbergs, Heinrich
Medical Journal of Indonesia Vol 13, No 4 (2004): October-December
Publisher : Faculty of Medicine Universitas Indonesia

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A total of 33 dairy lactating cows suffering from subclinical mastitis were classified into group A (10 cows), group B (12 cows) and group C (11 cows). They were treated with the combination of homeopathic drugs and placebo, applied at the 4th and 3rd week antepartum (a.p), and continuing every week postpartum (p.p), for four times. Group A received Coenzyme comp® (in the 4th and 3rd week a.p), Lachesis comp® combined with Traumeel®(1st and 2nd week p.p) and Coenzyme comp® combined with Carduus comp® (3rd and 4th week p.p). Group B received Traumeel®+ Mucosa comp® (4th and 3rd week a.p), Lachesis comp®+ Traumeel® (1st and 2nd week p.p) and Coenzyme comp®+ Carduus comp® (3rd and 4th week p.p) and group C as a placebo. The incidence of subclinical mastitis in group A and C appeared irregular. In contrast, group B showed a constant percentage (33.3%). Group A and B showed significant response to the homeopathic drugs, expressed as an increasing of the somatic cell count value. At peripartal phase, haptoglobin increased in group B 0.80 mg/ml and group A as well as placebo 1.40 mg/ml. After calving, group B expressed a constant value (0.05 mg/ml), while group A and placebo rose significantly. The milk yield in normal lactation period (the 3rd - 7th month) increased significantly, with an increasing 14.1% for group B and 4.9% A respectively. (Med J Indones 2004; 13: 221-6)Keywords: Homeopathy, Homeopathic combination, Subclinical Mastitis, Peripartal Phase
Konsentrasi Protein Total, Albumin, dan Globulin Anak Kambing Peranakan Etawah Setelah Pemberian Berbagai Sediaan Kolostrum* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O Esfandiari, Anita; Widhyari, Sus Derthi; Widodo, Setyo; Wibawan, I Wayan Teguh; Sajuthi, Dondin; Sutama, I Ketut
Jurnal Veteriner Vol 15, No 3 (2014)
Publisher : Jurnal Veteriner

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This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat) colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat), frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums) would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.
Efek Penambahan Mineral Zn Terhadap Gambaran Hematologi pada Anak Sapi Frisian Holstein Widhyari, Sus Derthi; Esfandiari, Anita; Wijaya, Agus; Wulansari, Retno; Widodo, Setyo; Maylina, Leni
Jurnal Ilmu Pertanian Indonesia Vol 19, No 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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The objective of this experiment was to determine the effects of zinc supplementation on health status in dairy calves. Nine Frisian Holstein (FH) at 6-10 months of age were used in this experiment and divided into three groups. First group (no added Zn) for control, the second group was added 60 ppm Zn, and the third group was added 120 ppm Zn. Zn was administered daily for three months. Blood samples were collected from the jugular vein and anticoagulated with EDTA. Whole blood were used for measuring erythrocytes, hemoglobin concentration, hematocrit value, total leukocyte count, and leukocyte cell types. The results showed that no difference among groups for hematological parameters and the value of hematology ​​were in the range values references. In conclusion, 60 and 120 ppm Zn supplementation in the feed is relatively safe for health. Keywords: dairy calves, erythrocyte, leukocyte, Zn mineral
Infusum Daun Alpukat Sebagai Inhibitor Kristalisasi Kalsium Oksalat pada Ginjal (THE AVOCADO LEAVES INFUSUM AS INHIBITOR ON RENAL CALCIUM OXALATE CRYSTALIZATION) Madyastuti, Rini; Widodo, Setyo; Wientarsih, Ietje; Harlina, Eva
Jurnal Veteriner Vol 16, No 4 (2015)
Publisher : Jurnal Veteriner

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Urine crystal is a crystal nucleus which tend to form urine stone. The case of urine stone seems to beincreased every year. Crystallization could induce acute tubular necrosis which impact on renal dysfunction.The signs of this condition are high level of urea, creatinine and decrease glomerulus filtration rate. Theobjective of this research was to evaluate the effects of infusum Persea americana Mill as an inhibitorcrystallization which induced by ethylene glycol on white male rats. 20 male rats were divided into 4groups; K1 as negative group received only distilled water ad libitum, K2 as positive group receiveddistilled water containing ethylene glycol, K3 (dose 5%) and K4 (dose 10%) as treatment groups receivedwater containing ethylene glycol and avocado leaves infusion. Phytochemsitry screening of infusion avocadoleaves consisted of flavonoid, saponin, tanine and quinone. Result of analysis showed that the level ofureum and creatinine on K2 was higher than K3 and K4 group. The increased level could be inhibited byinfusion avocado leaves. The measurement of glomerular filtration rate in treatment groups wassignificantly different (p<0.05). Descriptive histopathology observation showed that renal lesio in grouptreatment (K3 and K4) were declined. Large crystal calcium oxalate on K2 group was observed by usingpolarized microscope, whereas small crystal calcium oxalate were seen in the infusion of avocado leavesgroups. These result showed the ability of infusion of avocado leaves as an inhibitor on the growth ofcrystallization calcium oxalate
Efek Penambahan Mineral Zn Terhadap Gambaran Hematologi pada Anak Sapi Frisian Holstein Widhyari, Sus Derthi; Esfandiari, Anita; Wijaya, Agus; Wulansari, Retno; Widodo, Setyo; Maylina, Leni
Jurnal Ilmu Pertanian Indonesia Vol 19, No 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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The objective of this experiment was to determine the effects of zinc supplementation on health status in dairy calves. Nine Frisian Holstein (FH) at 6-10 months of age were used in this experiment and divided into three groups. First group (no added Zn) for control, the second group was added 60 ppm Zn, and the third group was added 120 ppm Zn. Zn was administered daily for three months. Blood samples were collected from the jugular vein and anticoagulated with EDTA. Whole blood were used for measuring erythrocytes, hemoglobin concentration, hematocrit value, total leukocyte count, and leukocyte cell types. The results showed that no difference among groups for hematological parameters and the value of hematology were in the range values references. In conclusion, 60 and 120 ppm Zn supplementation in the feed is relatively safe for health.
Tinjauan Penambahan Mineral Zn dalam Pakan Terhadap Kualitas Spermatozoa pada Sapi Frisian holstein Jantan Widhyari, Sus Derthi; Esfandiari, Anita; Wijaya, Agus; Wulansari, Retno; Widodo, Setyo; Maylina, Leni
Jurnal Ilmu Pertanian Indonesia Vol 20, No 1 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Bulls are expected to be able to produce a good quality and quantity of sperm. The objective of this experiment was to study the effect of Zn supplementation on the sperm quality, in Frisian holstein bulls. Ten bulls, 1618 months of age were used in this experiment. The experimental bulls were divided into two groups, i.e.,group without Zn supplementation (control) and group with 60 ppm of Zn supplementation. Zn supplementations were given everyday for a period of four months. Semen was collected by using artificial vaginaat the end of the experiment. Semen quality was evaluated macroscopically and microscopically. The variables measured were semen volume, semen pH, sperm motility, sperm concentration, sperm viability, and sperm abnormality. The results showed that Zn supplementation significantly increased sperm motility and sperm concentration (P<0.05), whereas there was no significant difference in other parameters.
KIVSA-4 Identifikasi Klinis Kristaluria pada Kasus Feline Lower Urinary Track Disease (FLUTD) di Klinik Hewan Maximus Pet Care Mihardi, Arief Purwo; Paramita, Intan Maria; Pakpahan, Sherli Noviaria; Widodo, Setyo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Feline lower urinary tract disease (FLUTD) terjadi karena adanya disfungsi dari kantung kemih maupun uretra pada kucing.  Salah satu simptom dari FLUTD yaitu polakiuria tanpa disertai poliuria, adanya stranguria dan hematuria (Gunn-Moore 2003; Westroop dan Buffington 2010).  Menurut Hostutler et al. (2005), hampir kebanyakan kucing yang mengalami LUTD terjadi karena terjadinya feline idiopathic, interstitial cystitis, urolitiasis, infeksi bakterial pada saluran urinari, malformasi anatomi saluran urinari, neoplasia, behavioral disorder, dan gangguan syaraf seperti refleks dysnergia.  Seperti yang dilaporkan Dorsch et al. (2014), dari 302 ekor kucing yang mengalami LUTD terdapat feline idiopathic cystitis (FIC) (55%), infeksi bakterial saluran urinari (18,9%), uretral plug (10,3%) dan urolithiasis (7%).  Kojrys et al. (2017) juga melaporkan 385 kucing yang mengalami LUTD terdapat 60,7% mengalami FIC, 17,4% obstruksi uretra akibat plug, 7,8% infeksi bakterial saluran urinari, 13% mengalami urolitiasis, 1 % terjadinya hiperplasia.Hampir sebagian besar kejadian LUTD diikuti dengan adanya obstruksi.  Menurut laporan Kojrys et al. (2017), FLUTD diikuti terjadi obstruksi uretra pada 229 kucing.  Umumnya obstruksi ini terjadi pada kucing jantan (204 ekor) dan hanya terdapat 25 ekor terjadi pada kucing betina.  Obstruksi ini biasanya terjadi pada kasus FIC yakni 129 ekor dan 67 ekor mengalami urolitiasis.  Menurut Osborne dan Lulich (2006), jenis kristal urin yang sering ditemukan pada kasus urolitiasis seperti struvit, kalsium oksalat, urat, sistin ataupun campuran.  Studi ini dilakukan untuk mengetahui karakteristik kristalurin yang terjadi pada 13 ekor kucing yang mengalami LUTD.