Irkham Widiyono
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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PROFIL FARMAKOKINETIK OKSITETRASIKLIN HIDROKLORID DALAM BERBAGAI JARINGAN TIKUS SPRAGUE DAWLEY Dwi Wijayanti, Agustina; Hakim, Lukman; Widiyono, Irkham
Jurnal Sain Veteriner Vol 25, No 2 (2007)
Publisher : Fakultas Kedokteran Hewan

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Telah dilakukan penelitian mengenai kadar oksitetrasiklin hidroklorida dalam hati, ginjal dan otot tikus Sprague Dawley jantan untuk mendapatkan profil farmakokinetik dalam jaringan tubuh. Tujuan penelitian ini adalah untuk mengetahui profil absorpsi, distribusi, metabolisme dan ekskresi oksitetrasiklin hidroklorida pada berbagaai jaringan tubuh yang berbeda. Metode penelitian yang digunakan adalah pengambilan sampel jaringan setelah tikus disuntik senyawa oksitetrasiklin secara intravena dengan dosist unggal 20 mg/kg berat badan. Organ hati, ginjal dan otot diambil setelah t kus dietanasi menggunakan eter pada menit ke 1, 5, 15, 20, 30, 60, 120 dan 240. Selanjutnya semua sampel jaringan disimpan dalam freezer(-20C) kemudian diekstraksi dan dianalisis serta diukur kadarnya menggunakan High Performance Liquid Chromatograph(HPLC). Parameter farmakokinetik obat ditentukan menggunakan model non kompartemen. Hasil penelitian menunjukkan bahwa terdapat perbedaan kadar, profil dan parameter farmakokinetik oksitetrasiklin hidroklorida pada hati, ginjal dan otot yang menunjukkan perbedaan sifat distribusi, metabolisme dan ekskresi obat.Kata kunci: oksitetrasiklin hidroklorida, profil farmakokinetik, jaringan.
Pengaruh Panhisterektomi dan Konsumsi Suplemen 1,25-Dihidroksivitamin D3 Selama 1,5 Bulan terhadap Retensi Kalsium pada Tikus Wistar -, Hartiningsih; Anggraini, Devita; Widiyono, Irkham
Jurnal Veteriner Vol 11, No 1 (2010)
Publisher : Jurnal Veteriner

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The objective of the research was to study the effect of panhisterectomy and 1,25-dihydroxyvitamin D3supplement consumption for 1.5 months on Ca retention of Wistar rats fed with casein. Twenty female ofWistar rats, 8 weeks of age were randomly divided into four groups (normal group N and NK,panhisterectomized group H and HK) in wich group consisted of 5 rats. Group N and H rats were fed witha standard diet, while group NK and HK rats were fed with a standard diet+1,25-dihydroxyvitamin D3supplement. At 19 weeks of age, they were placed into individual metabolic cages for balance studies.From day 7 to 11 of the balance studies, daily unconsumed food, urine, and feces were collected andrecorded for Ca analyses. The research results showed that Ca comsumption and fecal Ca excretion weresignificantly higher (P<0.05) in panhisterectomized rats consuming 1,25-dihydroxyvitamin D3 supplement,as compared with the normal rats consuming 1,25-dihydroxyvitamin D3 supplement. Urinary Ca excretionand Ca retention in panhisterectomized rats were not significantly different those of normal rats. WhileCa consumption and fecal Ca excretion were significantly reduced (P<0.05) in normal rats consuming 1,25-dihydroxyvitamin D3 supplement but urinary Ca excretion and Ca retention in normal rats consuming1,25-dihydroxyvitamin D3 supplement were not significantly different compare with normal rats notconsuming 1,25-dihydroxyvitamin D3 supplement. It is evident that panhisterectomy and 1,25-dihydroxyvitamin D3 supplement did not affect the calcium retention.
Penentuan Efektifitas Oksitetrasiklin Melalui Parameter Farmakokinetik/farmakodinamik pada Plasma dan Jaringan Ayam Broiler Dwi Wijayanti, Agustina; Hakim, Lukman; Widiyono, Irkham; Irianti, Tatang
Jurnal Veteriner Vol 11, No 2 (2010)
Publisher : Jurnal Veteriner

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A study was conducted to determine the effectiveness of oxytetracycline on the basis of itspharmacokinetic/pharmacodynamic (PK/PD) parameters in the plasma and tissues of broiler following a50 mg/kg single dose of intravenous administration. The male broiler were injected with oxytetracycline attarsal medial venous. The blood, liver, kidney and abdominal muscle were collected at 14 points of timesamplings. Blood was centrifuged to obtain plasma and all tissues were extracted with Mc Ilvine buffer.Drug concentration in samples was determined by High Performance Liquid Chromatography (HPLC)using AOAC standard reference for tetracycline. The pharmacokinetic parameters of oxytetracycline werecalculated using non compartment method. The results were as follows. For plasma: the AUC was 790,615,5 ?g/mL minute , Clearance was 63,242 mL/min/kg bb, Tmax/Cmax was 1 minute/0,7 ?g/mL, T1/2elimination was 6,4, hours, Vd was 37 L/kg. For liver ; AUC was 36418,89, minute , Tmax/Cmax was 16hours/17,15 ?g/g, and T1/2 elimination was 24,5, hours. For kidney; AUC6808,41 ?g/g minute , Tmax/Cmax 1 minute/16,73 ?g/g, , T1/2 elimination was 11,55 hours. For muscle; AUC was 3502 ?g/g minute,Tmax/Cmax was 1 minute/2,58 ?g/g, T1/2 elimination was 167,39 hours. The ratio of AUC/MIC inplasma and tissues appeared to a good pharmacokinetic/pharmacodynamic parameter to determine theeffectiveness of oxytetracycline.
Kadar Estradiol dan Progesteron Serum, Tampilan Vulva dan Sitologi Apus Vagina Kambing Bligon Selama Siklus Birahi Widiyono, Irkham; Putro, Prabowo Purwono; -, Sarmin; Astuti, Pudji; Airin, Claude Mona
Jurnal Veteriner Vol 12, No 4 (2011)
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A study was carried out to determine the serum estradiol and progesterone concentration, vulvaappearance, and vaginal cytology during estrous cycle in bligon goat. Four female non pregnant bligongoats (Indonesian indigenous) were used in this research. They were clinically healthy and showed normalestrous cycle. Estrous state was examined by using biological method. When the animals showed acceptanceto be mounted by a buck, they were confirmed as the first day of estrous cycle (estrous phase). Collectionof blood sample, preparation of vaginal smears, and measurement of some physical genital parameterswere conducted by standard veterinary clinical methods at day 11, day between 3-5, day between 6-16, andday between 17-19 of estrous cycle. Enzyme linked immunosorbent assay (ELISA) was used to determineserum progesterone and estradiol concentrations. Mean difference of each parameter was analyzed byanalyses of variance (Anova), followed by Tuckey HSD. The serum estradiol concentration ranged between211.25-247.77 pg/ml. Peak of the estradiol was occurred at the first day of estrous phase, namely 247.77pg/ml and to decrease at day 3-16. The serum progesterone concentration was at the level of 0.21-0.70 ng/ml and showed a significant increase at day 6-16 (p<0.05). The specific physical genital changes (swellingvulva, reddish mucous membrane of vulva, and excretion of viscous transparent vaginal discharge) wereidentified as the animals were at the estrous phase. Composition of exfoliative vaginal cytology did notshow any specific and consistent changes throughout the estrous cycle. Superficial and intermediate cellswere very dominant (>60%) during estrous cycle. In conclusion, serum estradiol, progesterone, the vulvaappearance combined with sexual receptivity could be a good estrous predictor for female cycling bligongoat.
Kemampuan Kelenjar Saliva untuk Mengkonsentrasikan Fosfat pada Kambing Widiyono, Irkham
Buletin Peternakan Vol 21, No 1 (1997): Buletin Peternakan Vol. 21 (1) April 1997
Publisher : Fakultas Peternakan UGM

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Kadar Mineral Makro dalam Serum Kambing Peranakan Ettawa yang Secara Klinis Sehat (SERUM MACROMINERALS CONCENTRATIONS IN CLINICALLY HEALTHY ETTAWA CROSSBRED GOATS) Widiyono, Irkham; ., Sarmin
Jurnal Veteriner Vol 13, No 2 (2012)
Publisher : Jurnal Veteriner

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A study to evaluate the serum macrominerals status in clinically healthy Ettawa crossbred goatshave been carried out in order to obtain the baseline data for monitoring health condition. A total of 60Ettawa crossbred goats were used in the study. The demographic characteristics of the animals groupwere: (i) young male and female goats (5-8 months); (ii) adult male and female goats (1.5 – 4 years); (iii)pregnant, and (iv) lactating animals. The serum macrominerals concentrations were within the referencestatus of other small ruminant species. The mean calcium (Ca), potassium (K), and chloride (Cl)concentrations were not significantly difference (P>0.05) between the animals groups. The Na concentrationof adult female goats was 139.33 ± 1.94mmol/L and was significantly lower compared to the animals inthe other groups (P<0.05). The inorganic phosphor (Pi) concentrations in pregnant and lactating animalswere significantly lower (P<0.05) compared to that in young, adult, male and female goats. The macromineralsconcentration in serum was influence by the physiological status of the animals.
Keterkaitan Panhisterektomi dan Suplemen 1,25- Dihidroksivitamin D3 dengan Risiko Urolitiasis pada Tikus (CORRELATION BETWEEN PANHISTERCTOMY AND 1.25-DIHYDROXYVITAMIN D3 SUPPLEMENTATION ON RATS UROLITHIASIS RISK) ., Hartiningsih; Anggraeni, Devita; Widiyono, Irkham; Wuryastuti, Hastari
Jurnal Veteriner Vol 13, No 3 (2012)
Publisher : Jurnal Veteriner

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The objective of this research was to study the correlation of panhisterectomy and supplement 1.25-dihydroxyvitamin D3 on urolithiasis risk in Wistar rats. Twenty female Wistar rats at 8 weeks of age, weredivided into four groups (control fed standard diet, control fed standard diet+1,25-dihydroxyvitamin D3 supplement, panhisterectomy fed standard diet and panhisterectomy fed standard diet +1,25-dihydroxyvitamin D3 supplement). Eleven weeks after treatment, each of rats was placed into individualmetabolic cage for balance study for a week. From day 4 to 11 of the balance study, every morning theremaining food, feces, and urine were collected and recorded for calcium (Ca) analysis. At the end ofbalance study, blood samples were taken from canthus retroorbitalis medialis for estrogen analysis. Theresults showed urinary and fecal Ca excretions were not significantly different compared to the controlgroup. Calcium consumption was significantly higher (P<0.05) in panhisterectomized rats compared withthose in control rats. While, estrogen in panhisterectomized group was not significantly different to thosein control rats. Calcium urinary and Ca consumption in rats consuming 1,25-dihydroxyvitamin D3 supplement were significantly higher (P<0.05) compared with those in without 1,25-dihydroxyvitamin D3 supplementation, but Ca excretion in feses was not significantly different. Estrogen in rats consuming1.25-dihydroxyvitamin D3 supplement was significantly lower (P<0.05) compared with the rats that without1,25-dihydroxyvitamin D3 supplemention. It can be concluded that panhisterectomy does not seem to affecturolithiasis risk, while 1,25-dihydroxyvitamin D3 supplement may affect urolithiasis risk. There is likelyno association between panhisterectomy and 1.25-dihydroxyvitamin D3 supplementation on urolithiasisrisk in Wistar rats.
STATUS MAKROMINERAL (Ca dAn P) DOMBA YANG TERINFESTASI RINGAN DAN BERAT CACING STRONGIL Purnamaningsih, Hary; Widiyono, Irkham; Titik Mulyani, Guntari
Jurnal Sain Veteriner Vol 25, No 2 (2007)
Publisher : Fakultas Kedokteran Hewan

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Infestasi parasit gastlointestinal adalah salah satu penyebab defisiensi makromineral. Penelitian ini bertujuan untuk mengevaluasi status makrominera(Ca dan P ) pada domba yang terinfestasi ringan dan berat oleh cacing strongil. Tiga puluh tujuh (37) ekor domba lokal, jantan, umur +12 bulan di daerah Sleman digunakan dalam penelitian ini. Domba di kelompokkan menjadi kelompok yang terinfestasi ringan( 26 ekor) dan kelompoky ang terinfestasbi erat (10 ekor) cacings hongil. Pengelompokkan domba didasarkan pada pemeriksaan parasitologi terhadap jumlah telur cacing per gram tinja. Setiap domba pada masing-masing kelompok diambil sampel darah sebanyak 5 mL melalui vena jugularis, selanjutnya dipisahkan plasmanya. Pengambilan sampel darah dilakukan sebelum domba digembalakan atau  diberi pakan. Plasma yang diperoleh disimpan pada suhu- 20C sampai analisis mineral dilakukan. Analisis Ca dalam plasma dilakukan dengan metode o-Kresolpthalein Komplekson yang diuraikan oleh Ray Sarkerd an Chaunan( 1967), dan fosfat anorganik dalam plasma diperiksa dengan menggunakan metodef osfomolibdat( Kraft dan Duer, 1999). Pemeriksaan jumlah telur cacing dilakukan secara mikroskopik dengan menggunakan metode McMaster. Hasil penelitian menunjukkan bahwa(1) 97% domba lokal, jantan, dewasa(± 12 bulan) di daerah Sleman terinfestasi cacing strongil, (2) infestasi ringan (< 1.000 epg) dan infestasi berat (> 1.000 epg) cacing strongil pada domba tampaknya tidak menimbulkan gejala klinis atau gangguan patologis yang dapat mengganggu metabolisme makromineral( Ca dan P), dan (3) stafus domba yang terinfestasi ringan tidak menunjukkan perbedaan yang nyata dengan domba yang terinfestasi berat dan status makrominerapl ada kedua kelompok tersebut masih dalam batas nilai fisiologisnorm(Ca:9,65±0,29 d an 10,60 ±.0,46m g/dl dan (P:6,61r0,25dan6,76±0,38mg/dl). Kata kunci: makromineral, cacings trongil, domba
Pengaruh Pemberian Teri Asin Terhadap Ekskresi Kalsium Urin dan Mineralisasi Tulang Femur Tikus Penderita ., Hartiningsih; Widiyono, Irkham; Wuryastuty, Hastari
Jurnal Sain Veteriner Vol 23, No 2 (2005)
Publisher : Fakultas Kedokteran Hewan

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PENGARUH KONSUMSI IKAN TERI TERHADAP MINERALISASI TULANG PADA TIMIS PUTIH (Rattus norvegicus albinus) = EFFECT OF "TERI" FISH CONSUMPTION ON BONEMINERALIZATION IN RATS (Railus nonvgicus albinus) Widiyono, Irkham; Wuryastuti, Hastari; ., Hartiningsih
Jurnal Sain Veteriner Vol 19, No 2 (2001): en
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Pengaruh konsumsi ikan teri terhadap mineralisasi tulang telah diteliti pada tikus dewasa normal (sehat) dan penderita osteodistrofia umur 4 bulan. Osteodisatrofia pada hewan penelitian ini diinduksi dengan pemberian pakan yang mengandung fosfor (P) tinggi dan kalsium (Ca) cukup. Pakan perlakuan (pakan teri) dibuat dari tepung jaeune._ tepung kedelai dan tepung teri yang memiliki kandungan Ca dan P sebanyak masing-masing 0,5% dan 0,65%. Kandungan ikan ten dalam pakan tersebut adalah 20% (b/b). Sebagai pakan kontrol adalah pakan yang tersusun dari tepung jaeuna_ tepune kedelai, CaCO3 dan NaH2PO4 dan mempunyai kandungan Ca dan P yang sama dengan pakan ten. Perlalcuan pakan berlangsung selama 2 bulan secara terus menerus. Pada minggu terakhir dilakukan studi balms Ca dan P. Pada hari terakhir penelitian dilakukan pengambilan sampel darah. Selanjutnya hewan dietanasi dan dilakukan koieksi tulang femur. Hasil pemeriksaan kimia darah, berat badan dan tulang femur (panjang, berat kering, densitas abu, mineralisasi tulang, kandungan Ca dan P) menunjukkan bahwa semua parameter dalam batasan normal dan tidak menunjukkan adanya perbedaan diantara kelompok perlalcuan pakan tersebut. Dari hasil penelitian ini dapat disimpulkan bahwa konsumsi ikan terimenimbulkan efek negatif terhadap metabolisme Ca dan P serta mineralisasi tulang, baik pada individu normal (sehat) maupun yang mengalami gangguan patologik tulang (osteodistrofia).