Dyah Widiastuti
Research and Development Animal Borne Diseases Control Unit, Banjarnegara

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GAMBARAN UMUM KASUS LEPTOSPIROSIS DI KOTA SEMARANG TAHUN 2004-2006 Widiastuti, Dyah; Djati, Rr. Anggun Paramita
Jurnal Vektor Penyakit Vol 2, No 1 Okt (2008)
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Study on Leptospirosis incidences during 2004 until 2006 was done in Semarang City. Secondary data about Leptospirosis incidences were collected from Semarang Health Office. The result showed that within three years, 2004-2006, Leptospirosis was occuring in Semarang City. A different proportion of the case was found between male and female which the male is predominantly (66%) transmitted with Leptospirosis. There were more cases found in lower area, than in upper area. Besides, the pattern of cases almost look like as the pattern of rainfall index.   Keywords: Leptospirosis, rainfall index, Semarang City.
PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10 Widiastuti, Dyah; Umniyati, Sitti Rahmah; Wijayanti, Nastiti
Balaba Vol 8, No 1 Jun (2012)
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ABSTRACTDengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infectedAedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae.aegypti, which should be the main targetof surveillance and control activities. Virologic surveillance for dengue viruses in its vector has been used as an earlywarning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash usingimmunocytochemical streptavidin biotin peroxidase complex (SBPC) assay is an alternative method for dengue vectorsurveillance. The study aimed to develope immunocytochemical SBPC assay to detect Dengue virus infection in headsquash of Ae.aegypti. The study design was experimental. Artificially-infected adult Ae. aegypti mosquitoes of DENV 3were used as infectious samples and non-infected adult Ae. aegypti mosquitoes were used as normal ones. Theimmunocytochemical SBPC assay using monoclonal antibody DSSE10 then was applied in mosquito head squash todetect Dengue virus antigen. The results were analyzed by descriptive analysis. The immunocytochemical SBPC assaycan detect Dengue virus antigen in mosquito head squash at day 2 postinfection. There are some false positive resultsfound in immunocytochemical SBPC assay.Key Word: Dengue, immunocytochemistry, DSSE10
SPOT SURVEY PENINGKATAN KASUS MALARIA DI DESA WONOHARJO WILAYAH PUSKESMAS ROWOKELE KABUPATEN KEBUMEN Widiastuti, Dyah; Djati, Anggun Paramita
Balaba Vol 8, No 2 Des (2012)
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ABSTRACTIntroduction : There was an increasing malaria cases on August 2011 in Wonoharjo village, Banyumasregency. 12 malaria cases were reported by Local Health Office. The aim of this study was to investigate theprevalence rate and the entomological situation in transmission area.Methods : Mass blood survey for microscopic diagnosis was conducted at Dukuh Beji and Lokarsa in Wonoharjovillage on 13-15 August 2011. Entomological survey using landing collection method and light traps wasconducted only at Dukuh Lokarsa in Wonoharjo village on 15-16 August 2011.Results : A total of 7 samples among 68 were positive by microscopy, giving a point prevalence of 10.3%. Thespecies distribution was 57.1% trophozoit Plasmodium falciparum (Pfr), 28,6% trophozoit and gametositP.falciparum (Pfrg) and 14.3% falciparum-vivax mixed infection. Increasing malaria cases was caused byimported cases in Wagirpadan village which located next to Wonoharjo village. The suspected mosquito vectorswere An.balabacensis and An.maculatus which caught from indoor and outdoor resting collection.Conclussions : Indigenous malaria transmission occured in Wonoharjo with the suspected vector wereAn.balabacensis and An.maculatus. The peak of mosquito bitting time was on 20.00 – 21.00 WIB. Migrationsurveillance need to be done effectively.
Dominant factors influencing leptospira sp infection in rat and suncus Ikawati, Bina; Sunaryo, Sunaryo; Widiastuti, Dyah
Health Science Journal of Indonesia Vol 3, No 2 Des (2012)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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AbstrakLatar belakang: Tikus dan cecurut dapat menularkan leptospirosis. Setiap jenis tikus dan cecurut mempunyai habitat masing-masing, antara lain jenis, daerah, dan ketinggian. Tujuan penelitian ini untuk menganalisis pengaruh ketinggian, variasi spesies, jenis tanah, tata guna lahan, dan banjir terhadap infeksi Leptospira sp pada tikus dan cecurut.Metode: Penelitian potong lintang dilakukan di beberapa lokasi pada bulan April-November 2011 di Jawa Tengah (kota dan kabupaten Semarang, kabupaten Demak, kabupaten Sleman dan Wonogiri). Tikus dan cecurut yang tertangkap diidentifikasi, dan diambil ginjal untuk diperiksa keberadaan bakteri leptospira dengan Polymerase Chain Reaction (PCR). Untuk mengidentifikasi faktor yang mempengaruhi infeksi leptosira sp pada tikus, analisis data memakai pendekatan risiko relatif.Hasil: Spesies tikus dan cecurut, tingkat elevasi dan jenis tanah berpengaruh terhadap risiko infeksi Leptospira sp. Dibandingkan dengan S. murinus, Rattus norvegicus spesies hampir 78 kali lipat berisiko infeksi Leptospira sp (risiko relatif rasio suaian (RRa = 77.87, P = 0,001], dan R. tanezumi memiliki risiko yang lebih rendah (RRa = 7,95, P = 0,048). Menurut tingkat elevasi tanah, elevasi yang rendah (0-100 m) dibandingkan dengan yang lebih tinggi (101-600 m) berisiko 2,3 kali lipat terinfeksi Leptospira sp (RRa = 2,27, P = 0,132).Kesimpulan:Infeksi leptospirosis pada tikus berkaitan dengan species tikus dan cecurut serta tingkat elevasi serta jenis tanah. (Health Science Indones 2012;2:xx-xx)Kata kunci:species tikus dan cecurut, leptospirosisAbstract Background: Mice and suncus may transmit leptospirosis. Each type mice and suncus had different habitat, among other, types, regions, and altitude. The purpose of this study to analyze the effect of altitude, variety of species, soil type, land use and flood against Leptospira sp infection in mice and sucus.Methods:A cross-sectional study conducted at several locations in April-November 2011 in Central Java (the town and district of Semarang, district of Demak, district of Sleman and Wonogiri). Mice and suncus caught were identified, kidney were taken for the existence of bacteria leptospira by Polymerase Chain Reaction (PCR). To identify factors affecting leptosira sp infection, analysis of data using relative risk approach.Results:Species of rats and suncus, elevation level, soil types and area related to risk Leptospira sp infection. Compared with S. murinus, Rattus norvegicusspecies had almost 78-fold to be Leptospira sp infected (adjusted relative risk ratio (RRa = 77.87; P = 0.001], and R. tanezumi had less risk (RRa = 7.95; P = 0.048). In term of elevation level, Lower elevation level (0-100 m) compared with higher level (101-600 m) had 2.3-fold risk to be Leptospira sp infection (RRa = 2.27; P = 0.132).Conclusion:Species of rats and suncus, elevation level, soil types and area related to risk Leptospira sp infection. (Health Science Indones 2012;2:xx-xx)Key words:Species of rats and suncus and leptospirosis
Mapping of leptospirosis risk factor based on remote sensing image in Tembalang, Semarang City, Central Java Sunaryo, Sunaryo; Widiastuti, Dyah
Health Science Journal of Indonesia Vol 3, No 1 Jun (2012)
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AbstrakLatar belakang: Leptospirosis merupakan penyakit zoonosis yang disebabkan oleh bakteri leptospira dan menular kepada manusia melalui kontak dengan urine hewan dan lingkungan yang terkontaminasi bakteri leptospira. Kecamatan Tembalang merupakan daerah endemis leptospirosis selama tiga tahun terakhir. Tujuan penelitian ini mengkaji kegunaan citra penginderaan jauh untuk pemetaan faktor risiko lingkungan leptospirosis.Metode: Penelitian ini menggunakan cara potong lintang, subyek sebanyak 246 dipetakan dengan GPS. Dengan program ArcGis 9.2 kasus leptospirosis ditumpang susun dengan citra Quickbird, kemudian dilakukan interpretasi kenampakan visual, dan dilakukan digitasi layar untuk identifi kasi faktor risiko secara visual.Hasil: Berdasarkan visualisasi digital diperoleh data bahwa kasus leptospirosis tahun 2009 terbanyak tersebar membentuk klaster di wilayah Tembalang dengan indeks jarak terdekat 0,009 km, sedangkan indeks jarak terjauh 18 km. Kasus lebih banyak ditemukan pada anak-anak dan remaja laki-laki, secara temporal kasus meningkat pada musim kemarau, antara bulan Juli dan Agustus. Hasil interpretasi visual dan digitasi diperoleh peta penggunaan lahan, badan air, pemukiman, area luasan banjir, kerapatan vegetasi dan ketinggian tempat.Kesimpulan: Citra penginderaan jauh resolusi spasial tinggi sangat baik untuk pemetaan faktor risiko leptospirosis. Sebaran kasus leptospirosis membentuk klaster di wilayah Tembalang, kasus didominasi anakanakdan remaja laki-laki. (Health Science Indones 2012;1:45-50)Kata kunci: citra penginderaan jauh, leptospirosis, Tembalang AbstractBackground: Leptospirosis is a zoonotic disease, caused by leptospira bacteria and transmitted to human by contact though contaminated animal urine and environment. Tembalang Sub District is endemic area ofleptospirosis and increased at last three years. The aim of this research was to study the ability and usefulness of image remote sensing for mapping as distribution of leptospirosis, physical environment of risk factor analysis.Methods: This cross sectional design consisted of 246 leptospirosis subjects mapped with GPS, and processed by using ArcGis 92 program. Leptospirosis case was overlaid with remote sensing (Quickbird image), then is done interpretation of spatial feature, and digitation on screen to visual identify of risk factor.Results: Based on digital visualization leptospirosis cases in 2009 were clustered in Tembalang with shortest distance index 0,009 km and is furthermost 18 km. More case distribution found at children and men adolescent. Temporally, case increased in the dry season, among of July and August. Result of visual interpretation and digitation can obtain land use map, water body, settlement, fl oods area, vegetation index and height.Conclusion: Spatial high resolution remote sensing image is very good for mapping of leptospirosis risk factor. Leptospirosis case distribution forms the cluster in Tembalang; case is predominated by children andmen adolescent. (Health Science Indones 2012;1:45-50) 
Several environmental factors related with endemicity status of dengue haemorrhagic fever Djati, Rr. Anggun Paramita; Widiastuti, Dyah
Health Science Journal of Indonesia Vol 3, No 1 Jun (2012)
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AbstrakLatar belakang: Faktor lingkungan mempengaruhi kejadian Demam Berdarah Dengue (DBD). Tujuan penelitian ini untuk mengidentifi kasi faktor-faktor lingkungan yang berkaitan dengan status endemisitas DBDdi Gunungkidul.Metode: Data dikumpulkan dengan studi potong lintang pada bulan Oktober-Desember 2010. Sampel rumah dipilih dengan multistage sampling. Sampel penelitian terdiri dari 155 rumah masing-masing daerah sporadic dan non-aporadik DBD. Data yang dilakukan dengan observasi langsung meliputi tipe rumah, jarak antar rumah, tinggi tanaman, luas tanaman, dan rimbunan tanaman. Risiko relatif (RR) diperhitungkan menggunakan Stata 9.Hasil: Rimbunan tanaman, tipe rumah, dan jarak antar rumah merupakan tiga faktor lingkungan dominan terhadap status endemisitas DBD. Jika dibandingkan daerah dengan area dengan rimbunan tanaman yangjarang, daerah dengan rimbunan tanaman sinambung merata memiliki risiko 2,5 kali menjadi endemis DBD [95% interval kepercayaan (CI) = 1.95-3.13]. Selanjutnya, area dengan jarak antara rumah yang sangatdekat dibandingkan dengan jarak antar rumah 10 m atau lebih memiliki risiko lebih tinggi menjadi daerah endemik.Kesimpulan: Semakin rimbun tanaman di sekitar rumah dan semakin dekat jarak antara rumah mempertinggi risiko status endemik DBD. (Health Science Indones 2012;1:27-30)Kata kunci: status endemisitas, DBD, lingkungan AbstrackBackground: Epidemiologically, environmental factors also affect the incidence of Dengue Haemorrhagic Fever (DHF). The purpose of this study was to identify environmental factors related to the endemicity statusof DHF in district of Gunungkidul, Yogyakarta.Methods: A cross sectional study has been done in endemic and sporadic area. Collected data conducted in October-December 2010. Samples of house were selected by multistage sampling. Samples of this study consisted of 155 houses of each area. Data collected by observation survey. The data were house type, distance between houses, plants height, plants area, and hedge plants. Relative risk (RR) was estimated using STATA 9 software.Results: Our fi nal model suggested that there were three risk factors (hedge plants, house type, and distance between the houses) were dominant risk factors for endemicity status of DHF. Compared with rare hedge plants area, area with hedge plants evenly continuous had 2.5 times risk to be endemic DHF [adjusted relative risk (RR) = 2.47; 95% confi dence interval (CI) = 1.95-3.13]. In term of house type, compared with temporary housing, semi-permanent as well as permanent houses were less risk to be endemic DHF area. In addition, compared with the area with distant between houses (10 m or more), the area with medium, near, as well as very near distant between houses had higher risk to be endemic area.Conclusion: More continuous hedge plants, and the closer the distance between the houses, increasing the risk of endemicity status of DHF. (Health Science Indones 2012;1:27-30) 
Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquito Widiastuti, Dyah; Yunianto, Bambang; Umniyati, Sitti Rahmah; Wijayanti, Nastiti
Health Science Journal of Indonesia Vol 2, No 2 Des (2011)
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Latar belakang: Survei virologi pada nyamuk vektor dapat digunakan sebagai Sistem Kewaspadaan Dini untuk mencegah penularan Demam dengue di suatu daerah. Pemeriksaan laboratoris untuk deteksi virus Dengue pada nyamuk seperti isolasi virus, Polymerase Chain Reaction (PCR) dan Direct Fluorescent-Antibody (DFA) memerlukan keahlian yang tinggi, peralatan yang mahal dan waktu yang lama. Suatu metode berdasarkan imunositokimia menggunakan antibody monoclonal DSSE10 memiliki beberapa kelebihan. Tujuan penelitian ini untuk mengevaluasi sensitifitas dan spesifitas pemeriksaan imunositokimia dibandingkan metode Reverse Transcription-Polymerase Chain Reaction (RT-PCR) untuk mendeteksi infeksi Virus Dengue 3. Metode: Penelitian eksperimental dilakukan di laboratorium Parasitologi Fakultas Kedokteran Universitas Gajah Mada (UGM) pada bulan Mei 2009-Oktober 2010. Sebanyak 22 Ae. aegypti yang diinfeksi virus Dengue 3 digunakan sebagai  kelompok  infeksius dan  35  nyamuk  yang  tidak  diinfeksi  sebagai  kelompok  non infeksius.  Pemeriksaan imunositokimia  Streptavidin  Biotin Peroxidase  Complex  (SBPC)  menggunakan  antibodi  monoklonal DSSE10 dilakukan pada sediaan head squash Ae .aegypti untuk mendeteksi antigen virus Dengue 3.  Pemeriksaan RT-PCR sebagai baku emas diaplikasikan pada toraks nyamuk.Hasil: Nilai Kappa menunjukkan kesepakatan yang baik antara dua orang pemeriksa (0,63). Imunositokimia mendeteksi antigen virus Dengue-3 dengan sensitivitas yang sama dengan RT-PCR (sensitivitas 100%). Namun spesifisitas IC lebih rendah dibanding RT-PCR (spesifisitas 91%) karena beberapa hasil positif palsu muncul pada pemeriksaan ini. Kesimpulan: Metode IC memiliki nilai sensitivitas dan spesifisitas yang tinggi dibandingkan dengan metode RT-PCR. Metode IC ini dapat digunakan untuk surveilans virus Dengue pada nyamuk vektor. (Health Science Indones 2011;2:87-91).AbstractBackground: Virological  surveillance  provides  an  early warning  sign  for  the  risk  of  transmission  in  an area. Laboratory  tests  for  dengue  virus  infection  on mosquitoes  include  isolation  of  the  virus,  Polymerase Chain Reaction  (PCR)  and  Direct  Fluorescent-Antibody (DFA)  requires  a  high  level  of  technical  skill, expensive equipment,  and  time-consuming.  A  method based  on  immunocytochemical  (IC)  using  monoclonal antibody DSSE10 has several advantages. This study aimed to evaluate sensitivity and specificity IC assay compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR) as gold standard to detect Dengue Virus (DENV)-3 infections in mosquito Aedes aegypti.Methods: An experimental study was conducted in laboratory of Medical Parasitology, Faculty of Medicine, Universitas Gadjah Mada (UGM) in May 2009 until October 2010. A total of 22 artificially-infected adult Ae.  aegypti  mosquitoes  of  DENV 3  were  used  as  infectious  samples  and  35  non-infected adult  Ae.  aegypti mosquitoes  were  used  as  normal  ones. The  IC  Streptavidin  Biotin  Peroxidase  Complex  (SBPC) assay  using monoclonal antibody DSSE10 was applied in mosquito head squash to detect Dengue virus antigen. RT-PCR as a gold standard was applied in mosquito thorax.Results:  The  kappa  value  showed  a  good  agreement between  two  observers  (kappa  value  0.63).  IC could detect dengue virus antigen as sensitive as RT-PCR (sensitivity 100%). But IC was less specific than RT-PCR (specificity 91%) because some false positive results were found in this method.Conclusion: The IC method has a high sensitivity and high specificity compared with RT-PCR. This IC method may  be useful  for  virological  surveillance  of  dengue  infected Aedes  mosquitoes.  (Health  Science  Indones 2011;2:87-91). 
Angiostrongylus cantonensis Widiastuti, Dyah
Balaba Vol 7, No 1 Jun (2011)
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Bagi Anda yang termasuk pelahap sayuran mentah (lalapan), harus cukup berhati-hati dengan agen penyakit berikut ini. Nama latinnya adalah Angiostrongylus cantonensis, salah satu jenis cacing Nematoda yang juga sering dikenal dengan nama rat lungworm, penyebab utama dari penyakit eosinophilic meningitis. Sebagaimana Nematoda lainnya, cacing ini juga memiliki bentuk filiform (seperti benang). Cacing jantanya berukuran ±7,7 mm dengan diameter 0,30 mm, sedangkan cacing betina ± 12,8 mm dan diameter 0,36 mm. Organ genitalia pada cacing  jantan berupa bursa kopularis sedangkan cacing betina berupa vulva yang terletak di ujung posterior. Pada bagian kepala terdapat 3 buah labia, 2 diantaranya terletak di bagian dorsal, sedang yang 1 buah terletak di bagian lateraf.
Trichinella spiralis, Cacing yang Menginfeksi Otot Astuti, Novia Tri; Widiastuti, Dyah
Balaba Vol 5, No 1 Jun (2009)
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Trichinella spiralis merupakan salah satu jenis nematoda/cacing gilig. Cacing ini tersebar di seluruh dunia (kosmopolit), terutama daerah beriklim sedang. Trichinella spiralis menyebabkan penyakit yang disebut trichinosis, trikinelosis, dan trikiniasis. Selain menginfeksi manusia, cacing ini juga menginfeksi mamalia lain seperti tikus, kucing, anjing, babi, beruang dan lain-lain.
KEANEKARAGAMAN JENIS NYAMUK Anopheles DI DAERAH DENGAN ATAU TANPA KEBUN SALAK DI KABUPATEN BANJARNEGARA Widiastuti, Dyah; Yunianto, Bambang; Ikawati, Bina
Balaba Edisi 002 No.01/Tahun II Juni 2006
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Anopheles has been known as vectors of malaria and filaria. A research which was aimed to evaluate the diversity of Anopheles mosquitoes at Salak and Non Salak Areas was conducted. This Research located in Banjarnegara Regency which lay in a mountainous in the middle of Central Java (712"- 731 N and 10920"-10945"W). The location was divided into two groups i.e. (1) Kendaga Village (Banjarmangu Subdistrict) representing of Salak area, (2) Badakarya Village (Punggelan Subdistrict) representing Non Salak area. Mosquitoes were collected by landing and resting collection methods. All mosquitoes were anaesthetized with chloroform and identified under microscope. Shanon-Weaver Index and Eveness Index were measured to evaluate the diversity of Anopheles mosquitoes. The results showed there were 6 species of Anopheles from both areas i.e. A. aconitus. A. balabacensis. A. barbirostris. A. kochi. A. vagus and A. maculatus. Result of examination by Independent Sample T-Test indicated that the diversity index value between two areas were not significantly different. Keywords: Anopheles, diversity, salakarea, non salakarea