Dyah Ayu Widiasih
Bagian Kesmavet FKH UGM Yogyakarta

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Kajian Kasus-kontrol Avian Influenza Pada Unggas di Jawa Timur, Jawa Tengah dan Daerah Istimewa Yogyakarta= A Case-control Study on Avian Influenza in Poultry in East Java, Central Java and Yogyakarta Special Province. Widiasih, Dyah Ayu; Susetyo, Heru; Sumiarto, Bambang; Tabbu, Charles Rangga; Budiharta, Setyawan
Jurnal Sain Veteriner Vol 24, No 1 (2006)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Kajian kasus-kontrol yang dirancang untuk menyidik kejadian avian influenza (Al) dan mencari hubungannya dengan faktor resiko penyakit, telah dilakukan terhadap 218 dusun di Jawa Timur, Jawa Tengah dan Daerah Istimewa Yogyakarta. Sebagai kasus (109 dusun) adalah dusun yang pernah dilaporkan atau sedang mengalami kasus AI, dan kontrol (109 dusun), adalah dusun yang dilaporkan belum pernah mengalami, tetapi dekat dengan dusun kasus. Kuesioner digunakan untuk menjaring variabel yang diperkirakan berasosiasi dengan kejadian AI. Data yang diperoleh dianalisis dengan Chi Square (x2) dan odds ratio (OR). Hasil kajian menunjukkan bahwa faktor adanya hewan pengerat (OR = 1,90), faktor adanya burung liar (OR = 24,00), faktor pekerja pulang sehabis kerja (OR = 2,65), dan faktor sektor III (OR = 1,79) mempunyai asosiasi karat dengan kejadian AI di suatu dusun, sedangkan beberapa faktor biosekuriti berasosiasi lemah (OR = 1,0 – 1,5) terhadap kejadian Al.
The Analysis of Amount and Various Age of Productive Female Bali Cattle that Slaughtered at Abbatoirs Suardana, I Wayan; Sukada, I Made; Suada, I Ketut; Widiasih, Dyah Ayu
Jurnal Sain Veteriner Vol 31, No 1 (2013)
Publisher : Fakultas Kedokteran Hewan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.662 KB)

Abstract

Abstract Base on the ability for surviving at the limited vegetations, bali cattle is famous  as a pioneer cattle.  Although the fertility of Bali cattle has  known   so high (up to 80%), but  the slaughter  of productive  female  of bali cattle from year to year is so high too, so that the existance of Bali cattle in the future is threatened extinct. The accurate data  indicating  the amount of Bali cattle slaughtered  at  the abbatoirs are  not available yet, exactly from the Pesanggaran and Mambal  abbatoirs as  the bigger abbatoirs in Bali.  The study used 246 heads of  Bali cattle originated from Pesanggaran, and 232 heads of  Bali cattle originated from Mambal abbatoirs, respectively. The study indicated as many as   81,7%, and 87,5% of Bali cattle slaughtered  at  those abbatoirs  were female. According to their  ages, most of them were  productive too, i.e.  99% at Pesanggaran, and 67,49% at Mambal abbatoirs, respectively. These  result  indicated  it is needed a  special attention from the Bali government exactly from the Animals Husbandry Officer in order to prevent the loss of Bali cattle populations  in the future.    
Penentuan Marka Genetik Escherichia coli O157:H7 Asal Hewan dan Manusia dengan Metode Random Amplified Polymorphic DNA (GENETIC MARKERS IDENTIFICATION OF ESCHERICHIA COLI O157:H7 ORIGINATED FROM ANIMALS AND HUMAN BY USING RANDOM AMPLIFIED POLYMORPHIC DNA Suardana, I Wayan; Widiasih, Dyah Ayu; Pinatih, Komang Januartha Putra
Jurnal Veteriner Vol 15, No 3 (2014)
Publisher : Jurnal Veteriner

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Abstract

The use of random amplified polymorphic DNA (RAPD) as a method to identify a genetic markerof bacteria is widely used by researcher. This method is known as a simple, faster, and reliabletechnicque. This study is to find out the aplication of RAPD method in order to identify specific markersof E. coli O157:H7 as a zoonotic agent. The study began by cultivating of 20 isolates of E. coli O157:H7colected by previous study that consist of 2 isolates originated from cattle feces, 2 isolates originatedfrom beef, 2 isolates originated from chicken feces, 2 isolates originated from healthy human and 11isolates originated from unhealthy human (human with kidney failure). All isolates were confirmed byculturing on selective medium sorbitol MacConkey agar (SMAC). Confirmation were followed by testingon O157 latex aglutination, and finally by testing on H7 antiserum. RAPD method as molecularanalysis was performed using decamer primers mixture OPA-01, OPA-02, OPA-03, and OPA-04.Results of study showed both bands 1721 and 700 bp are specifically to differentiate of isolatesoriginated from cases of healthy and unhealthy human. On the other hand, bands with position 1721 bp,300 bp, and 250 bp indicate the isolates originated from unhealthy human, healthy human and chicken,respectively. Isolates from beef are characterized by both bands 1400 and 429 bp, and the isolates fromcattle feces are identified by band with position 342 bp. The specific bands are considered as markers inorder to know the source of E. coli O157:H7 fastly.
The Analysis of Amount and Various Age of Productive Female Bali Cattle that Slaughtered at Abbatoirs Suardana, I Wayan; Sukada, I Made; Suada, I Ketut; Widiasih, Dyah Ayu
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.662 KB) | DOI: 10.22146/jsv.2642

Abstract

Abstract Base on the ability for surviving at the limited vegetations, bali cattle is famous  as a pioneer cattle.  Although the fertility of Bali cattle has  known   so high (up to 80%), but  the slaughter  of productive  female  of bali cattle from year to year is so high too, so that the existance of Bali cattle in the future is threatened extinct. The accurate data  indicating  the amount of Bali cattle slaughtered  at  the abbatoirs are  not available yet, exactly from the Pesanggaran and Mambal  abbatoirs as  the bigger abbatoirs in Bali.  The study used 246 heads of  Bali cattle originated from Pesanggaran, and 232 heads of  Bali cattle originated from Mambal abbatoirs, respectively. The study indicated as many as   81,7%, and 87,5% of Bali cattle slaughtered  at  those abbatoirs  were female. According to their  ages, most of them were  productive too, i.e.  99% at Pesanggaran, and 67,49% at Mambal abbatoirs, respectively. These  result  indicated  it is needed a  special attention from the Bali government exactly from the Animals Husbandry Officer in order to prevent the loss of Bali cattle populations  in the future.    
Kajian Kasus-kontrol Avian Influenza Pada Unggas di Jawa Timur, Jawa Tengah dan Daerah Istimewa Yogyakarta= A Case-control Study on Avian Influenza in Poultry in East Java, Central Java and Yogyakarta Special Province. Widiasih, Dyah Ayu; Susetyo, Heru; Sumiarto, Bambang; Tabbu, Charles Rangga; Budiharta, Setyawan
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1791.726 KB) | DOI: 10.22146/jsv.341

Abstract

Kajian kasus-kontrol yang dirancang untuk menyidik kejadian avian influenza (Al) dan mencari hubungannya dengan faktor resiko penyakit, telah dilakukan terhadap 218 dusun di Jawa Timur, Jawa Tengah dan Daerah Istimewa Yogyakarta. Sebagai kasus (109 dusun) adalah dusun yang pernah dilaporkan atau sedang mengalami kasus AI, dan kontrol (109 dusun), adalah dusun yang dilaporkan belum pernah mengalami, tetapi dekat dengan dusun kasus. Kuesioner digunakan untuk menjaring variabel yang diperkirakan berasosiasi dengan kejadian AI. Data yang diperoleh dianalisis dengan Chi Square (x2) dan odds ratio (OR). Hasil kajian menunjukkan bahwa faktor adanya hewan pengerat (OR = 1,90), faktor adanya burung liar (OR = 24,00), faktor pekerja pulang sehabis kerja (OR = 2,65), dan faktor sektor III (OR = 1,79) mempunyai asosiasi karat dengan kejadian AI di suatu dusun, sedangkan beberapa faktor biosekuriti berasosiasi lemah (OR = 1,0 – 1,5) terhadap kejadian Al.
Isolasi dan uji kepekaan Escherichia coli O157:H7 Isolat lokal asal feses dapi terhadap berbagai jenis antibiotika Suardana, I Wayan; Suarsana, I Nyoman; Wibowo, Michael Haryadi; Widiasih, Dyah Ayu
Jurnal Sain Veteriner Vol 29, No 2 (2011): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5938.951 KB) | DOI: 10.22146/jsv.39512

Abstract

Escherichia coli O157:H7 is one of the bacteria infection agents that life threatening.