I WAYAN TEGUH WIBAWAN
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan Institut Pertanian Bogor

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PEMANFAATAN TELUR AYAM SEBAGAI PABRIK BIOLOGIS (KAJIAN PUSTAKA) WIBAWAN, I WAYAN TEGUH
Majalah Ilmiah Peternakan Vol 11, No 1 (2008)
Publisher : Majalah Ilmiah Peternakan

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ABSTRAK Penelitian tentang pemanfaatan telur sebagai pabrik biologis dilakukan sangat komprehensif di beberapa institusi perguruan tinggi di Inonesia, yakni Fakultas Kedokteran Hewan (FKH) Institut Pertanian Bogor (IPB), FKH Universitas Gadjah Mada (UGM), FKH Universitas Udayana (UNUD), FKH Universitas Syahkuala (Unsyiah), Fakultas Kedokteran Gigi Universitas Indonesia. Jejaring penelitian dibangun dengan baik dan telah menghasilkan beberapa produk, antara lain: telur berkhasiat flu burung H5N1, telur Anti Tetanus Serum, Telur Anti Diare, Telur anti Plaque dan Telur Anti White Spot Syndrome Virus pada udang. Secara ilmiah khasiat IgY spesifik dalam kuning telur sebagai senyawa therapeutic telah diuji dan tinggal memerlukan sentuhan akhir tersendiri untuk dapat disajikan sebagai produk komersial. Peran industri yang relevan sangat dibutuhkan dalam mewujudkan hal ini. THE USE OF EGG AS A BIOLOGICAL PRODUCER FOR SPESIFIC ANTIBODIES ABSTRACTS The use of egg as a biological producer for specific antibodies were done intensively among well known universities in Indonesia, such as Faculty of Veterinary Medicine (FVM) of FVM of IPB, FVM of UGM, FVM of UDAYANA and FVM of UNSYIAH as well as Faculty of Dental Medicine Indonesia University. The collaboration and research networking among those institutions run as expected and succeed to produce some important products such as eggs containing IgY anti avian flu as well as anti tetanus, anti diarrhea, anti plaques and eggs anti WSSV in shrimp. The potency of specific IgY as immunotherapeutic substances had been studied and need the commercial touch from the respective industries until this products in market available.
Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA) Purnawarman, Trioso; Wibawan, I Wayan Teguh; Pasaribu, Fachriyan Hasmi; Setiyono, Agus; Saepulloh, Muharam
Jurnal Veteriner Vol 13, No 1 (2012)
Publisher : Jurnal Veteriner

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Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.
Uji Patogenisitas Zoospora Kapang Lagenidium giganteum terhadap Larva Instar-2 Nyamuk Aedes aegypti Skala Laboratorium (PATHOGENICITY TEST OF ZOOSPORA LAGENIDIUM GIGANTEUM FUNGI AGAINST AEDES AEGYPTI LARVAE 2nd UNDER LABORATORY CONDITION) Indrawati, Agustin; Sudarwanto, Mirnawati; Tampubolon, Mangaraja Pidoli; Soejoedono, Retno Damayanti; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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Dengue Haemorrhagic fever (DHF) is one of fearsome diseases in society. Incidence of the disease isincreasing. Dengue fever is caused by dengue virus and transmitted by Aedes aegypti mosquito vector.Various chemical controls have been conducted to prevent the spread of the disease, but active contents ofthe chemical controlling substances are suspected causing many negative effect, in environment, such asvector resistance, death of non target living creatures, and environmental contamination.This researchobjective was to find an alternative solution in order to control the dengue vector by using entomopathogenicfungi as biological control agent. This research was conducted by isolation and identification of fungiinfecting mosquito larvae. Macroscopic observation revealed that one of the nine isolation products wasLagenidium giganteum. The effectiveness test in laboratory showed the zoospore LD50 to Ae.aegypti larvaeof instar 2nd was 2,35 x 106 zoospore/ml, while the LD95 value was 1,35 x 107 zoospore/ml. The oosporeeffectiveness test showed LD50 was 6,7 x 102 oospore/ml and LD95 was 1,94 x 103 oospore/ml. Using LPCBdye and blue tolouidin 2,5%, the infection mechanism of L.giganteum fungi in Ae.aegypti mosquito larvawas detected. The research is concluded that the entomophatogen fungi L. giganteum was very prospectiveto be used as a biological control agent against vector of DHF.
PHYLOGENETIC AND ANTIGENIC STRUCTURE OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM WATERFOWLS Susanti, R; Damajanti Soejoedono, Retno; Kade Mahardika, I Gusti Ngurah; Wibawan, I Wayan Teguh; Thenawidjaja Suhartono, Maggy
Jurnal Veteriner Vol 9, No 3 (2008)
Publisher : Jurnal Veteriner

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A study was carried (1) to analyze the phylogenetic relationship of fragment hemaglutinin (HA) geneof avian influenza viruses (AIV) subtype H5N1 isolated from apparently healthy backyard waterfowls inWest Java with representative of animal and human isolates from Indonesia and some countries in Asia;(2) to find out cross-reactivity of those viruses with a standard Indonesian strain. Nucleotide sequences ofHA gene of AIV H5N1 from backyard waterfowls along with other H5N1 isolates of Indonesian and Asianorigin were aligned using with ClustalW of MEGA 3.1 program. Estimation of genetic distance and theconstruction phylogenetic tree were conducted by Neighbor Joining method and calculation of distancematrix using Kimura 2-parameter. Antigenic analysis was conducted using hemagglutination inhibition(HI) test. Result of phylogenetic analysis indicated that all viruses from backyard waterfowls form threedistinct sublineages. One lineage was located in Indonesia cluster and two lineages in Asia cluster. In thephylogenetic analysis, it was concluded that multiple introductions of AIV H5N1 to Indonesia have occurred.Six AI H5N1 viruses from backyard waterfowls (IPB1-RS to IPB6-RS) appeared to be different ancestorsthose isolated previously in Indonesia. Cross-antigenic analysis showed that nine viruses isolates used inthis study were antigenically different to Legok 2003 chicken strain of AIV H5N1. The HI titer of anti-Legok 2003 antibody with all newly isolated viruses is up to 6 log lower then the HI titer using homologstrain.
Neutralization Ability of Specific Antibody of Avian Influenza H5 to Several Viruses of H5N1 Field Isolates Angi, Andrijanto H.; Wibawan, I Wayan Teguh; Murtini, Sri
Forum Pasca Sarjana Vol 32, No 1 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Avian Influenza (AI) is well known as Avian flu, Fowl pest, Fowl plaque, or Flu burung, caused by influenza virus type A.  This virus is belonged to Orthomyxoviridae and could infect many kind of species such as bird, pig, horse, cat, as well as human.  Vaccination is applied to control the disease using inactivated vaccine, which induced the specific antibody against H5 antigen.  Passive immunization using specific antisera against H5 antigen is thought to be usefull in controlling the disease especially in the treatment of infected host.  In this experiment the neutralization ability of specific antisera against H5 were studied using various field viral isolates subtype H5N1.  Antisera was developed in Cavia porcellus which vaccinated with AI subtype H5N1 in activated vaccine.  The titre of antisera obtained is 28 used HI test.  Four AI virus subtype H5N1 isolates from 2003 to 2006 agains viral were we as tested virus.  The neutralization test showed that the sera were able to neutralizing 10 4 EID50 AI virus H5N1 with neutralization index range of 1.1-1.3.  The result indicated that the specific antisera had the neutralization potency to the field virus.   Key words: avian influenza, neutralization test, neutralization index
Hemagglutination Activities of Streptococcus Agalactiae Isolates on The Animal and Human Erythrocytes Utama, Iwan Harjono; Kendran, Anak Agung Sagung; Wibawan, I Wayan Teguh; Pasaribu, Fachriyan Hasmi
Media Veteriner Vol 7, No 2 (2000): Media Veteriner
Publisher : Media Veteriner

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Hemagglutination activities and phenotypic expressions of fifty-five S. agalactiae isolates consisted of 19 standard and 36 field isolates from subclinical mastitis cattle were observed. Five (eighteen); 3 (4); 2 (7); 1 (2); and 5 (11) isolates (numbers in brackets indicated field isolates) were able to hemagglutinate cattle, horse, sheep, chicken, and human erythrocytes, respectively. The distribution of hemagglutination pattern was discissed in this paper.
Relation Between Encapsulation of Streptococci of Serological Group B and Adherence Properties of The Bacteria to Deae-Sephacel Lammler, Christoph; Wibawan, I Wayan Teguh; Pasaribu, Fachriyan H
Media Veteriner Vol 5, No 4 (1998): Media Veteriner
Publisher : Media Veteriner

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Determination of surface charge of group B streptococci by ion exchange chromatography on DEAE-Sephacel revealed that bovine and human group B streptococcal isolates with protein surrface antigens alone, or bacteria with protein antigen in combination with polysacharide antigens, adhered strongly to the gel matrix. In contrary, cultures with polysacchaide antigens alone showed no comparable adherence properties. Removal of neuraminic acid from bacterial surface enhanced, but pronase treatment reduced the adherence values. The importance of type specific capsular sialylation for group B streptococcal surface charge could be confirmed with group B streptococci of serotype III and their transposon mutagenized asialocapsular mutants. In contrary to the encapsulated parent strains the asialo capsular mutants adhered strongly to the gel matrix. Comparable differences were observed with unencapsulated group B streptococcal variant strains and its isogenic encapsulated parent strains. The capsule material seemed to mask the surface proteins responsible for the adherence to the gel matrix. The determination of surface charge of group B streptococci by ion exchange chromatography might help to understand the importance of capsular sialylation for individual isolates of this bacterial species.
ISOLASI DAN KARAKTERISASI HEMAGLUTININ Staphylococcus aureus PENYEBAB MASTITIS SUBKLINIS PADA SAPI PERAH Abrar, Mahdi; Wibawan, I Wayan Teguh; Priosoeryanto, Bambang Pontjo; Soedarwanto, Mirnawati; Pasaribu, Fachriyan Hasymi
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.462 KB) | DOI: 10.21157/j.ked.hewan.v6i1.347

Abstract

Dalam penelitian ini isolasi dan karakterisasi hemaglutinin Staphylococcus aureus dilakukan dengan teknik afinitas kromatograf Karakterisasi hemaglutinin yang dihasilkan dilajutkan dengan teknik elektroforesis menggunakan metode sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) dan dilanjutkan untuk melihat pengaruh suhu dan enzim terhadap aktivitas hemaglutinin. Hasil penelitia menunjukkan bahwa komponen hemaglutinin Staphylococcus aureus yang telah diisolasi memiliki berat molekul 46 kDa. Aktivit Staphylococcus aureus dalam meghemaglutinasi hilang pada pemanasan 60° C dan pengaruh enzim proteolitik. Hasil ini mengindikasika bahwa hemaglutinin Staphylococcus aureus adalah protein.
Development of Rapid Agglutination Test to Detect Chicken Marek Antibody WIBAWAN, I WAYAN TEGUH; HALIMAH, LIA SITI; DJANNATUN, TITIEK; ZARKASIE, KAMALUDDIN
Microbiology Indonesia Vol 3, No 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

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To our knowlege, there is no rapid agglutination test to detect antibodies to viruses which might be due to the small dimension of viral particles. Through complex formation of Staphylococcus aureus bearing protein A-rabbit IgG-anti IgY-IgY anti Marek virus, agglutination of antibodies to viruses can be achieved and visualized. To design the prototype of the test, the bacterial cells of Staphylococcus aureus were coupled to a complex compound consisting of IgG-IgY-Marek antigen. This protocol was able to detect clearly the presence of Marek antibody in chicken sera, showing the rapid, clear and distinct agglutination reaction on the glass objects. No agglutination reaction was observed in the reaction of specified pathogen free chicken sera with this prototype showing the specificity of the test. This finding demonstrates a novel rapid agglutination which can be used for the detection of antibodies to various agents.
Vaksin Polivalen Untuk Mencegah Penyakit Flu Burung (POLIVALEN VACCINE TO PREVENT BIRD FLU DISEASES) Suartha, I Nyoman; Wirata, I Wayan; Putra, I Gusti Ngurah Narendra; Dewi, Ni Made Ritha Krisna; Anthara, I Made Suma; Wibawan, I Wayan Teguh; Mahardika, I Gusti Ngurah Kade
Jurnal Veteriner Vol 13, No 2 (2012)
Publisher : Jurnal Veteriner

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This study was carried out to determine the use of bird flu polyvalent vaccines containing two or threeor more virus isolates representating of circulating viruses in the region. Three seed isolates of avianinfluenza H5N1 virus were used in this experiment. The isolates were Chicken/Denpasar/Unud-01/2004,Chicken/Klungkung/Unud-12/2006, and Chicken/Jembrana/Unud-17/2006. The seeds were inactivatedusing 0.01% formaldehide than mixed (AI3G) alumunium hidroxide adjuvant and then injectedintramuscularly to Isa Brown layer chicken at 3 weeks of age and repeated at the age of 5 weeks. The doseof each seed virus was 27 HA units. Sera were collected at one and two weeks after the second vaccination.The result showed that the arithmetic meant titer (AMT) of sera that tested with homologous isolate washigher than the test using a heterologous isolates, in the standard haemaglutination inhibition (HI) assay.The mixed AI3G vaccine produced a uniform AMT against the constituent isolates, while vaccines withindividual isolate yielded a lower and more variation in AMT. Further experiments using a commercialhomologous H5N1 and heterologous H5N2 commercial vaccines has resulted AMT that 1-4 log lower thanAI3G vaccine. It is concluded that polyvalent vaccine with field seed isolates is recommended to be appliedin the poultry farm in Indonesia.
Co-Authors Agik Suprayogi Agnesia Endang Tri Hastuti Wahyuni Agus Setiyono AGUSTIN INDRAWATI Ahmad Sulaeman Aida Louise Tenden Rompis Aisjah Girindra Anak Agung Sagung Kendran Andrijanto H. Angi Anita Esfandiari Asep Gunawan, Asep BAMBANG PONTJO PRIOSOERYANTO BAMBANG PONTJO PRIYOSOERYANTO Budi Setiawan Cece Sumantri Christoph Lammler Darusman, Huda Salahuddin Denny Widaya Lukman DEWI APRI ASTUTI DONDIN SAJUTHI Endhie D. Setiawan, Endhie D. Etih Sudarnika Fachriyan H Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Pasaribu, Fachriyan Feri Kusnandar Gatut Ashadi Hadri Latif Harlystiarini, Harlystiarini Hendri Latif, Hendri HERA MAHESHWARI I Gusti Ayu Agung Suartini I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra I Ketut Sutama I Made Suma Anthara I Nyoman Suartha I Wayan Wirata Indrawati Sendow Iwan Harjono Utama Kallau, Novalino Harold Geoffrey Kamaluddin Zarkasie Kamil Riski Sidik, Kamil Riski Kereh, Veybe Gresje LIA SITI HALIMAH Luwito, Bagus Nanang Maggy Thenawidjaja Suhartono Mahdi Abrar Mangaraja Pidoli Tampubolon Maria Fatima Palupi Mayasari, Ni Luh Putu Ika Michael Haryadi Wibowo Mirnawati Soedarwanto MIRNAWATI SUDARWANTO Mohammad Ashraf, Mohammad Muharam Saepulloh Muhsinin, Muhammad Muladno . Nahrowi Nahrowi Ni Luh Putu Agustini, Ni Luh Putu Ni Made Ritha Krisna Dewi Niken Ulupi Nurbani Kalsum R Susanti Rachmawati, Faidah Rachmawati Retno Damajanti Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Wulansari Rita Mutia Setyo Widodo Siti Gusti Ningrum, Siti Gusti Sri Murtini Sudarwanto, Mirnawati Baharudin Sus Derthi Widhyari Sylvia Oscarina, Sylvia TITIEK DJANNATUN Trioso Purnawarman Wyanda Arnafia, Wyanda