I Putu Agus Hendra Wibawa
UPT Balai Konservasi Tumbuhan Kebun Raya “Eka Karya” Bali LIPI

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UJI DAYA HAMBAT EKSTRAK DAUN KEDONDONG (LANNEA GRANDIS ENGL) TERHADAP PERTUMBUHAN BAKTERI ERWINIA CAROTOVORA Sudira, I Wayan; Merdana, I Made; Wibawa, I Putu Agus Hendra
Buletin Veteriner Udayana Vol. 3 No. 1 Pebruari 2011
Publisher : Buletin Veteriner Udayana

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Research with the title of the inhibition test kedondong leaf extract (Lannea grandisENGL)on the growth of bacteria Erwinia carotovora, causes soft rot of Aloe vera have beenconducted in vitro on media PPGA and testing of antibacterial activity of leaf extractkedondong on aloe vera leaf pieces. This study aims to determine the bactericidal activityof leaf extract and concentration kedondong minimal constraints on the growth ofbacteria Erwinea carotovora. Bacteria E. Carotovora isolated from the Aloe Vera plant softrot disease.Sections of leaves among the sick and the healthy cut to the size of ± 3 cm andcleaned with water and then soaked with 70% alcohol for 2 minutes inserted into laminar flow. Leaves clean kedondong have weighed as much as 100 grams chopped, then addedwith 1000 ml of methanol solvent. Immersion extract was filtered with filter paper watmanNo. 2.The filtrate obtained was evaporated by vacuum rotary evaporator to separate thesolvent (methanol) and the extract, made up to concentration. Bacteria isolated on mediapropagated PPGA tilted as stock for testing bacterial pathogens isolated colony of yellowwhitecolor with the aroma of sulfur-like smell of gas.Based on research results, it can beconcluded that the leaf extract could inhibit bacterial growth kedondongE carotovora withdoses at least 4% concentration in media PPGA and pieces of aloe vera leaves, whereas atconcentrations of 1, 2 and 3% have not been able to provide inhibition.
Association of Spheranthus africanus (Asteraceae) with other Weeds In a Fallowed Rice Field Priyadi, Arief; Wibawa, I Putu Agus Hendra; Lugrayasa, I Nyoman
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

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Spheranthus africanus is a minor weed of rice, which can be utilized for various purposes. It can be used for phytoremediation of soil contaminated with Pb and also reported to have anti-bacterial activity to be used as natural pesticides. However, because of its minority status little is known about this species in its natural habitat. This study was aimed to understanding the relationship between S. africanus with other weed species in a fallowed paddy field in Tabanan District, Bali, Indonesia. Vegetation analysis by quarter method was done in the rice field, where the species had occurred, in 2011 and 2012. Determination of association type between S. africanus with other weed was conducted by using 2x2 contingency table to calculate χ2, association coefficient (AC) and Ochiai index (OI). The result shows that the Shannon diversity index in this same area for the two consecutive years were 2.06 and 2.48 with species richness of 22 and 27 respectively. Calculation of Summed Dominance Ratio (SDR) showed that the species composition was not evenly distributed, in other words some species dominated others. In terms of association, although S. africanus mainly has no association with most of the weed species in the field, it prominently has positive association with Lindernia ciliata, and Digitaria ciliaris, with no significant negative association. Keywords: diversity, species association, Spheranthus africanus
Plant Collections of Bali Botanic Garden Potentially Used As Organic Pesticides Wibawa, I Putu Agus Hendra; Priyadi, Arief
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

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Along with society awareness of the importance of healthy life, demand of agricultural products which are pesticide residues free has been increasing. Recently, efforts to control plant pests and diseases by farmers have been relied on synthetic pesticides. Utilization of this kinds of pesticides results in various diseases such as cancer, fetal defects, infertility, liver diseases, and so on.  One of the alternatives which can be chosen to minimize those negative effects is using organic pesticides. Its ingredients are organic compounds which easily degraded, do not pollute the environment and relative safe for humans and livestock because its residues are degradable. Indonesia is one of countries with biggest biodiversity in the world, so it has great opportunities to produce organic pesticides. Bali Botanic Garden (BBG) is a government institution which plays role to conserve various kinds of plants from their natural habitats, especially the threatened species. This activity was aimed at knowing how many plant collections in BBG which have potency as organic pesticides and their specific functions whether as fungicides, insecticides, bactericides, rodenticides, nematicides, molluscicides, or fish poisons. The results show that there were 23 plants which have potency as organic pesticides. From that group, 22 kinds act as insecticides, 7 as fungicides, 5 as nematicides, 3 as molluscicides, rodenticides, fish poisons (pissicides), and bactericides respectively.Keywords: organic pesticides, Bali Botanic Garden, plant collections Keywords: organic pesticides, Bali Botanic Garden, plant collections
STUDI KERAGAMAN JENIS, KANDUNGAN GIZI ESENSIAL DAN KALSIUM OKSALAT DIOSCOREA DI PULAU BALI DAN LOMBOK Wibawa, I Putu Agus Hendra; Kurniawan, Agung; Adjie, Bayu
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) Juli 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Dioscorea tuber has many benefits, for example as food or as traditional medicine. The high carbohydrate and protein contents make this plant suitable as a food. However, the presence of crystal oxalate, that causes irritation is an disadvantage aspect on the development of this species. Both of wild and cultivated species will assure the gene flow which is important in efforts to improve the tuber quality should be examine further.  This research aimed to observe the biodiversity of Dioscorea in Bali and Lombok, to recognize the utilization and to reveal their nutrients (carbohydrate, protein and fat) and also the contents of calcium oxalate. The results showed that there are five Dioscorea spesies, namely  Dioscorea alata L. (ubi) (11 cultivars), Dioscorea esculenta (Lour.) Burkill (ubi aung), Dioscorea hispida Dennst. (gadung), Dioscorea bulbifera L. and Dioscorea pentaphylla L.   The highest carbohydrate content (87,42% ) is found in D. alata (ubi ungu cultivar) from Lombok,  while the highest protein content (5,05%.) is found in D. alata (ubi dadap cultivar) from Singaraja, Bali.  Furthermore, the highest percentage of fat content (0,85%) is found in D. bulbifera from Karangasem, Bali, whereas the high quantity of glaucomannan (44,52%.) id found in D. pentaphylla from Tabanan, Bali, while D. esculenta from Jembarana, Bali has the  highest calcium oxalate (0,57%), and D. alata (ubi injin cultivar) from Karangasem, Bali has the least calcium oxalate (0,18%).
STUDI KERAGAMAN JENIS, KANDUNGAN GIZI ESENSIAL DAN KALSIUM OKSALAT DIOSCOREA DI PULAU BALI DAN LOMBOK Wibawa, I Putu Agus Hendra; Kurniawan, Agung; Adjie, Bayu
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) July 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Dioscorea tuber has many benefits, for example as food or as traditional medicine. The high carbohydrate and protein contents make this plant suitable as a food. However, the presence of crystal oxalate, that causes irritation is an disadvantage aspect on the development of this species. Both of wild and cultivated species will assure the gene flow which is important in efforts to improve the tuber quality should be examine further.  This research aimed to observe the biodiversity of Dioscorea in Bali and Lombok, to recognize the utilization and to reveal their nutrients (carbohydrate, protein and fat) and also the contents of calcium oxalate. The results showed that there are five Dioscorea spesies, namely  Dioscorea alata L. (ubi) (11 cultivars), Dioscorea esculenta (Lour.) Burkill (ubi aung), Dioscorea hispida Dennst. (gadung), Dioscorea bulbifera L. and Dioscorea pentaphylla L.   The highest carbohydrate content (87,42% ) is found in D. alata (ubi ungu cultivar) from Lombok,  while the highest protein content (5,05%.) is found in D. alata (ubi dadap cultivar) from Singaraja, Bali.  Furthermore, the highest percentage of fat content (0,85%) is found in D. bulbifera from Karangasem, Bali, whereas the high quantity of glaucomannan (44,52%.) id found in D. pentaphylla from Tabanan, Bali, while D. esculenta from Jembarana, Bali has the  highest calcium oxalate (0,57%), and D. alata (ubi injin cultivar) from Karangasem, Bali has the least calcium oxalate (0,18%).
Taking inventory of Orchid Types in a Region of Tambora Mountain - Sumbawa Island Wibawa, I Putu Agus Hendra; Peneng, I Nyoman; Priyadi, Arief
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Indonesia is well known as one of the countries with high biodiversity in the world, including its orchids. It is expected that there are 6,000 kinds of orchids which grow wildly in forest regions around the world. Along with accelerated forest destructions in Indonesia, due to land conversions or natural disasters, the existence of orchids in their natural habitats is threatened. It is likely that some of these richness could be lost before they can be conserved and known of their functions. Regions of Tambora mountain in Sumbawa island have biodiversity that had not been revealed. Therefore, taking inventory of flora in that region is very crucial to be done as soon as possible. This activity was aimed at saving biodiversity especially rare and endemic orchids there, and also quantitatively knowing richness and frequency of orchids species which are found. From the research, it is known that there were 22 orchids species, from which only one is terrestrial and the rests are epiphytes. In terms of richness, there were some orchids which were common and also rare. Keywords: orchids, Tambora Mountain, conservation, exploration
Uji Efektivitas Ekstrak Mimba (Azadirachta indica A. Juss.) untuk Mengendalikan Hama Penggerek Daun pada Tanaman Podocarpus neriifolius WIBAWA, I PUTU AGUS HENDRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.1, Januari 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The effectiveness analysis of neem plant (Azadirachta indica A. Juss.) to control leaf borer on plant Podocarpus neriifolius Plant maintenance and development cannot be avoided from pests and diseases. Losses due to pest or disease attacks encourage patients to control variousefforts. So far the pest and disease control efforts carried out are still based on synthetic pesticides. The use of various types of synthetic pesticides, on the other hand bring problems to the environment such as causing poisoning to humans, killing non-target organisms, secondary pest outbreaks, pest resistance, soil, water and air pollution. One alternative pest control that can be done is by organic pesticides. One of the plants reported to have the ability to control pests is the neem plant (Azadirachta indica). This study aims to determine the effectiveness of neem extract in controlling leaf borer caterpillar on Podocarpus neriifolius compared to vegetable pesticides Dane brand and synthetic pesticides. The results of the research conducted, it was found that the most effective dose of mimba extract was 80 g/liter. Both wet and dry extracts are equally effective in controlling pests. Control using dry leaf extract with dose of 80 g/liter is more effective than using the Dane brand pesticide in three times the dose.
Pengaruh Konsentrasi Pupuk Cair (Mikroba Fungsional Tahan Salin) Terhadap Perkembangan Stek Daun Begonia rex “Silver Circle” WIBAWA, I PUTU AGUS HENDRA; LUGRAYASA, I NYOMAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.2, April 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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 The Effect Of Liquid Fertilizer Concentration On Growths Of Leaf Cuttings Begonia Rex "Silver Circle"   Begonia is one of the large genera in the Angiosperm group with asymmetrical leaf characteristics that are very suitable for ornamental plants. "Silver Circle" is the most popular type of Begonia rex. To ensure the availability of Begonia plants in large quantities, there is a need for propagation and cultivation.  The success of plants propagation by cuttings is influenced by various factors, namely the type of cuttings and environmental factors including temperature and light humidity and nutrients from the growing media. The quality of planting media can be improved by functional microbial liquid fertilizer. Liquid fertilizer containing microbes functional can produce nutrients and growth hormones that can support plant growth. This study aims to determine the effect of liquid fertilizer concentration given, and also to determine the optimal liquid fertilizer concentration on the growth of B. rex leaf cuttings "Silver Circle". The treatment of liquid fertilizer gave significantly different results from controls on the parameters of leaf area and plant height. The most optimal dose of liquid fertilizer is 300 mL/L.
PERBANDINGAN EFEKTIFITAS EKSTRAK LEGUNDI (Vitex trifolia L.), SIRSAK (Annona muricata L.) DAN DAMAR (Agathis borneensis Warb.) TERHADAP MORTALITAS LARVA Artona flavipuncta HAMA PADA TANAMAN KECOMBRANG (Etlingera elatior (Jack) R.M.Sm.) Wibawa, I Putu Agus Hendra
Agro Bali Vol 2, No 1 (2019)
Publisher : Universitas Panji Sakti

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Kecombrang (Nicolaia speciosa) adalah sejenis tumbuhan rempah yang dimanfaatkan sebagai bahan campuran atau bumbu penyedap pada berbagai macam masakan di Nusantara. Kecombrang adalah salah satu koleksi tumbuhan Kebun Raya Bali yang sering terserang oleh larva A. flavipuncta. Penanggulangan hama menggunakan pestisida sintetis menimbulkan banyak dampak negatif terhadap manusia dan lingkungan. Salah satu alternatif yang dapat digunakan adalah menggunakan pestisida nabati yang bersumber dari tanaman. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas beberapa tanaman koleksi Kebun Raya Bali seperti legundi (Vitex trifolia), sirsak (Annona muricata) dan damar (Agathis borneensis) terhadap mortalitas larva A. flavipuncta dan menentukan konsentrasi ekstrak yang paling efektif dalam membunuh larva. Hasil penelitian menunjukkan bahwa ekstrak yang paling efektif membunuh larva A. flavipuncta adalah ekstrak daun sirsak dengan konsentrasi optimum 60 g/L.
PERBANDINGAN EFEKTIVITAS BEBERAPA PESTISIDA ORGANIK PADA BUIDAYA BROKOLI (Brassica rapa L.) DI BEDUGUL, BALI Wibawa, I Putu Agus Hendra
Agro Bali Vol 1, No 1 (2018)
Publisher : Universitas Panji Sakti

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Pemeliharaan dan pengembangan tanaman tidak bisa dihindarkan dari serangan hama dan penyakit. Kerugian akibat serangan hama dan penyakit mendorong dilakukannya berbagai usaha pengendalian. Selama ini usaha pengendalian hama dan penyakit masih bertumpu pada penggunaan pestisida sintetis. Penggunaan pestisida sintetis dapat mengakibatkan masalah bagi manusia dan lingkungan. Seiring dengan meningkatnya kesadaran masyarakat akan pola hidup sehat, mengakibatkan meningkatnya permintaan produk sayur dan buah yang bebas dari residu pestisida sintesis. Salah satu alternatif pengendalian hama penyakit yang dapat dilakukan adalah dengan menggunakan pestisida nabati. Pestior merupakan merk dagang pestisida nabati yang dikembangkan di Kebun Raya Bali, terbuat dari bahan alami sehingga tidak mencemari lingkungan dan aman bagi manusia karena bahan aktifnya bersifat mudah terurai. Penelitian ini bertujuan untuk mengetahui efektivitas Pestior dalam mengendalikan hama pada tanaman Brokoli, dibandingkan dengan pestisida nabati lain yang biasa digunakan oleh para petani di Bedugul, Bali. Hasil penelitian menunjukkan bahwa terdapat perbedaan nyata dalam jumlah gigitan hama antara perlakuan satu dengan yang lainnya. Gigitan terbanyak dialami oleh perlakuan Pestior dosis 100 ml/L air, berbeda nyata dengan perlakuan Bumbu Bali dengan jumlah gigitan terbanyak kedua. Jumlah gigitan yang paling sedikit dialami oleh Pestior dosis 200 ml/L air yang tidak berbeda nyata dengan perlakuan Turex. Hal ini diduga terjadi karena dosis bahan aktif yang terdapat pada Pestior dosis 200 ml/L air dapat mencegah gigitan atau membuat hama tidak berselera untuk memakannya karena efek racun dalam perutnya.