Gustav A. Wattimena
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Pemetaan Gen Resistensi Lapang terhadap Penyakit Hawar Daun Kentang ~ pada Populasi Fl Solanum tuberosum (SH2988) x Solanum microdontum (MCD167) Tutupary, J. M.; Wattimena, Gustav A.; Aswidinnoor, Hajrial; Muladno, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 32, No 3 (2004): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

Potatoes can be severely affected by late blight, a fungal disease caused by Phvtophthora infestans. which can destroy the foliage and tubers of the crop. Initial attempts to control late blight in potato deployed resistance genes (R genes) derived from the hexaploid Solanum demissum (RI - Rll). However, their immunity reaction can be easily overcome by the development of new virulent factors. It is currently accepted that the most effective solution against late blight involve the use offield-resistant varieties as part of an integrated pest management strategy. Field resistance to Phvtophthora i!!festans were examined in a diploid segregating potato population. The population produced crosses between Solanum microdontum genotype (MCDI67) and ~ tuberosum genotype (SH2988) have been assessed for resistance to f.. infestans (late blight) in the field and prol1ed to segregate for f.. infestans resistance. A genetic linkage map of this population was constructed by using PCR-based CAPS markers. The result showed that one QTL on foliage resistance to late blight were linked with marker GP 180 on chromosome 4 of potato in coupling phase. Key words: CAPS markers, Field resistance, Late blight, Phvtophthora infestans. potato, QTL
Karakterisasi Morfologi Bunga dan Buah Abnormal Kelapa Sawit (Elaeis guineensis Jacq) Hasil Kultur Jaringan Hetharie, Helen; Wattimena, Gustav A.; Thenawidjaya S., Maggy; Aswidinnoor, Hajrial; Toruan-Mathius, Nurita; Ginting, Gale
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 1 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Tissue culture-derived plants of oil palm (Elaeis guineensis Jacq) can produce abnormal female dan male flowers, especially female, which develop into mantled fruit. This abnormality have caused yield lost up to 40%. The objectives of this research were to study the stages of flower development in which the abnormality started to appear, to characterize the morphology of the abnormal flower and fruit, and to determine the degree of abnormality. Plant materials used in this research were MK152, MK 176, MK 203, MK163, MK104, MK 212 and MK 209 clones obtained from the collection of "Badan Pengkajian dan Penerapan Teknologi (BPPT)" in Ciampea, Bogor. Characterization of abnormality at the flowering stage was conducted  based on morphological performance at several stages of flower development, while the degree of abnormality at fruiting stage was determined based on the condition of carpel, mesocarp and seed. Normal flower and fruit of the same clone were used as the control. The results showed that the abnormality started at primordial stage of flower organ formation. Supplement carpel was found surrounding gynoecium for the abnormal female flower, which was composed of three to six carpels present at  separate whorl of flower. Abnormal male  flower had no additional whorl of  flower, instead stamens were converted into carpel-like structures. In this case, number of carpels structure depends on the degree of abnormality. There were three levels of abnormality, i.e. light abnormality (AbR), heavy abnormality (AbB) and severe abnormality (AbSB). The severe abnormal fruit was seedless, with soft mesocarp (AbSB1) and woody mesocarp (AbSB2).   Key words : oil palm, mantled fruit, supplement carpel, flower whorl, tissue culture
Karakterisasi Morfologi Bunga dan Buah Abnormal Kelapa Sawit (Elaeis guineensis Jacq) Hasil Kultur Jaringan Hetharie, Helen; Wattimena, Gustav A.; Thenawidjaya S., Maggy; Aswidinnoor, Hajrial; Toruan-Mathius, Nurita; Ginting, Gale
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 1 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Tissue culture-derived plants of oil palm (Elaeis guineensis Jacq) can produce abnormal female dan male flowers, especially female, which develop into mantled fruit. This abnormality have caused yield lost up to 40%. The objectives of this research were to study the stages of flower development in which the abnormality started to appear, to characterize the morphology of the abnormal flower and fruit, and to determine the degree of abnormality. Plant materials used in this research were MK152, MK 176, MK 203, MK163, MK104, MK 212 and MK 209 clones obtained from the collection of "Badan Pengkajian dan Penerapan Teknologi (BPPT)" in Ciampea, Bogor. Characterization of abnormality at the flowering stage was conducted? based on morphological performance at several stages of flower development, while the degree of abnormality at fruiting stage was determined based on the condition of carpel, mesocarp and seed. Normal flower and fruit of the same clone were used as the control. The results showed that the abnormality started at primordial stage of flower organ formation. Supplement carpel was found surrounding gynoecium for the abnormal female flower, which was composed of three to six carpels present at ?separate whorl of flower. Abnormal male? flower had no additional whorl of? flower, instead stamens were converted into carpel-like structures. In this case, number of carpels structure depends on the degree of abnormality. There were three levels of abnormality, i.e. light abnormality (AbR), heavy abnormality (AbB) and severe abnormality (AbSB). The severe abnormal fruit was seedless, with soft mesocarp (AbSB1) and woody mesocarp (AbSB2). ? Key words : oil palm, mantled fruit, supplement carpel, flower whorl, tissue culture
Pemetaan Gen Resistensi Lapang terhadap Penyakit Hawar Daun Kentang ~ pada Populasi Fl Solanum tuberosum (SH2988) x Solanum microdontum (MCD167) Tutupary, J. M.; Wattimena, Gustav A.; Aswidinnoor, Hajrial; Muladno, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 32, No 3 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Potatoes can be severely affected by late blight, a fungal disease caused by Phvtophthora infestans. which can destroy the foliage and tubers of the crop. Initial attempts to control late blight in potato deployed resistance genes (R genes) derived from the hexaploid Solanum demissum (RI - Rll). However, their immunity reaction can be easily overcome by the development of new virulent factors. It is currently accepted that the most effective solution against late blight involve the use offield-resistant varieties as part of an integrated pest management strategy. Field resistance to Phvtophthora i!!festans were examined in a diploid segregating potato population. The population produced crosses between Solanum microdontum genotype (MCDI67) and ~ tuberosum genotype (SH2988) have been assessed for resistance to f.. infestans (late blight) in the field and prol1ed to segregate for f.. infestans resistance. A genetic linkage map of this population was constructed by using PCR-based CAPS markers. The result showed that one QTL on foliage resistance to late blight were linked with marker GP 180 on chromosome 4 of potato in coupling phase. Key words: CAPS markers, Field resistance, Late blight, Phvtophthora infestans. potato, QTL