R Wasito
Fakultas Kedokteran Hewan Universitas Gadjah Mada

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The Pathological Study of Suspected Swollen Head Syndrome in Broiler Chickens Sutrisno, Bambang; Widyarini, Sitarina; Wasito, R; Wuryastuty, Hastari
Jurnal Sain Veteriner Vol 31, No 1 (2013)
Publisher : Fakultas Kedokteran Hewan

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The  objective of this study was to determine the pathological lesions of the broiler chickens having the clinical signs that appeared to be the swollen head syndrome (SHS),  such as simetrically and bilaterally swollen periorbital areas. Eighteen broiler chickens were collected from three poultry farms of the three diffrerent areas, e.g. Kulonprogo, Sleman and Bantul at Special Province of Yogyakarta, respectively. Six of those broiler chickens were selected from each poultry farm consisted of three SHS- broiler chickens and three non SHS-broiler chickens. All of broiler chickens were necropsied, observed anatomic patologic lesions, and then the organs of interest were processed and stained routinely with hematoxylin and eosin for histopathologic examination. Subcutaneus edema and cellulitis were found in the frontal and occipital skin. Based on the histopathological examination, purulent and granulomatous cellulitis and lymphocytic perivasculitis, and subcutaneus edema were found in the frontal and occipital skin. Other lesions, such as: necrotic, lymphocytic sinusitis, tracheitis and pneumonia accompanied by atrophy of mucosal glands were also present. It is concluded that the pathological lesions of SHS in the broiler chickens were found not only in the frontal and occipatal skin, but also in both lower and upper respiratory tracts.      
Cytotoxic Tobacco Smoke in The Cornea of Wistar Rats Fed Turmeric (Curcuma Domestica Val.) Extract Kusumawardani, Agapietta; Sarwendah, Kurniati; Rahmad, Laelatul; Milah, Nida Ul; Herliyani, Novi; Sutrisno, Bambang; Wuryastuty, Hastari; Wasito, R
Jurnal Sain Veteriner Vol 31, No 1 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract An increasing number of smokers who tend to rise rapidly from year to year has become a major focus of public health issues. Exposure to environmental tobacco smoke, containing toxic chemicals can trigger acute or chronic inflammation and oxidative stress in the eyes. To overcome this condition, phytotherapeutic and/or phytopreventive approaches which are efficatious, safe and cheap is needed. One of spices from Indonesia which is greatly possible to be promoted as a herbal medicine, is a phytochemical curcumin in turmeric. This present study was conducted to identify and determine the effects of turmeric extract (Curcuma domestica Val.) as phytotherapeutic and/or phytopreventive in the eyes lesion of Wistar rats induced by cigarette smokes. Twenty male Wistar rats at 3 months old were divided randomly into four groups of five each. Those were the control group (KI), the group given 9 cigarettes smoke/day (K II), the group given turmeric extract 1 ml 0, 25% /day (K III) and the group given 9 cigarettes smoke/day and 0, 25% turmeric extract 1 ml /day (K IV) for 60 days. Water and feed was given ad libitum. At the end of the experiment, all rats were euthanized with ether, necropsied andeyes balls were collected, fixed formalin 10% and stained with routine hematoxylin-eosin. The results of the present study indicated that 9 cigarettes smoke/day increased mitotic activity and caused hyperplasia in the corneal epithelial cells (K II), and addition of turmeric extract 0, 25% 1 ml per oral for 60 days prevented both mitotic activity and hyperplasia in the corneal epithelial cells (K IV). Extract of turmeric (Curcuma domestica Val.) has been proved to have herbal therapeutic and/or preventive effects againts the pathotoxicological cigarette smokes. Further study needs to be done to more evaluate pathogenesis of the eyes lesions and phytotherapeutic and/or phytopreventive effects of turmeric extract as well.   
The Effects of Turmeric Extract (Curcuma domestica Val) on Cigarette Smoke Cardiotoxicity in The Wistar Rats Millah, Nida Ul; Kusumawardani, Agapietta; Rahmad, Laelatul; Herliyani, Novi; Sarwendah, Kurniati; Sutrisno, Bambang; Wuryastuty, Hastari; Wasito, R
Jurnal Sain Veteriner Vol 31, No 2 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract            The objective of this research is to determine the effects of turmeric (Curcuma domestica Val) extract administration toward heart histopathologic lesions of male albino Wistar rats + 3 months old exposed by cigarette smoke. Twenty male Wistar rats were divided randomly into four groups of five each: Group I was given neither cigarette smoke exposure nor turmeric extract administration (control) (KI), Group II was given cigarette smoke exposure of 9 cigarettes/ day (KII), Group III was given 1 ml turmeric extract 0.25% (KIII) and Group IV was given cigarette smoke exposure of 9 cigarettes/ day and 1 ml turmeric extract 0.25% (KIV). After 2 months of treatment, necropsy was conducted to all rats and the heart samples were collected to be processed histopatologically and stained routinely with hematoxylin-eosin. The results of the present study indicated that the cigarette smoke caused cardiomyopathy in KII marked by the histopathologic lesions such as vacuolation, homogeneous eosinophilic cytoplasms, edema and necrosis of myocardia, also congestion and mild hemorrhages (KII). Whereas, rats in Groups KI, KIII and KIV had normal histologic structures of myocardia. It is concluded, that turmeric extract could be a preventive herbs toward cardiotoxic effects of cigarette smoke. Further research has to be conducted to determine the anti cardiomyopathy mechanism of turmeric rhizome due to cigarette smoke exposure.
ANALYSIS OF COLFFORM BACTERIA IN THE WELL WATER AND ITS POSSIBLE BIOPATHOLOGIC EFFECTS Wuryastuti, Hastari; Wasito, R; Chalimah, Siti; Andayani, Sri; Indraswati, Yuni; Lestariyadi, Leksono; K, Prapti; Amien, Mohammad
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan

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Coliform is a Gram-negative, rod shape bacteria Some strains, especially Escherichia coli can cause diarrhea among animals aad humans. Contamination of water whith pathogenic E. coli has been associated with this disease in animals and humans.In the present study, coliform bacteria were determined with most probable number (MPN) method cultured on lactose broth and brillian green bile broth. Results indicated that the well water samples collected from the areas of Karangmalang, Sungai Code and Deresan had 150 coliform/100 ml, 210 coliform/100 ml and 460 coliform/l00 ml, respectively. Whereas, standard number of coliform organisms in drinking water recommended by World Health Organization (WHO) is 10 coliform/100 ml and is 1 E. coli/100 ml. Therefore, based on the present study, the coliform organisms content in the well water is for above its normal level value as recommended by WHO. Use of the water for daily consumption, especially as drinking water needs to be a great concern
The Effect of Turmeric (Curcuma Domestica Val.) Extract on the Histopathological Lesions of Lung Induced by Cigarette Smokes in Wistar Rats Rahmad, laelatul; Milah, Nida Ul; Kusumawardani, Agapietta; Herliyani, Novi; Sarwendah, Kurniati; Sutrisno, Bambang; Wuryastuti, Hastari; Wasito, R
Jurnal Sain Veteriner Vol 33, No 1 (2015)
Publisher : Fakultas Kedokteran Hewan

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This present study is aimed to observe and determine the effects of turmeric (Curcuma domestica Val.) extract administration towards the cytotoxicity effect of cigarette smokes in the lungs of Wistar rats. Twenty Wistar rats, male, 3 months old, were used and divided randomly into four groups, of five each. Group I is as a control (K I), Group II was given 9 cigarettes/day without turmeric extract administration (K II), Group III giventurmeric extract 1 ml 0.25%/day (K III) and Group IV was given 9 cigarettes/day and turmeric extract 0.25%/day (K IV) for 60 days. At the end of the present study, all rats were necropsied and organs, particularly the lungs were collected, processed histopatologically and stained routenely with hematoxilyn eosin. The results indicated that the lungs had macrophages infiltration in the septal alveoli and hyperplasia of bronchial associated lymphoidtissue (K II). Whereas, the lungs in the rats in Groups K I, K III and K IV were still within the normal limits. It is concluded, that turmeric extract may have a potential antipulmonary cytotoxity effect due to cigarette smoke.
Follicular Development Dynamics and Plasma Progesterone Profile during the Estrous Cycle of Dairy Cows Putro, PP; Wasito, R; Wuryastuty, H; Indarjulianto, S
ANIMAL PRODUCTION Vol 10, No 2 (2008): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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A total of five local Friesian cows, 4 to 5 years, healthy, reproductively sound, were used in the study to understand the development dynamics of dominant follicles and plasma progesterone profile during estrous cycle.   Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz transducer was performed daily for one full cycle to follow the development of dominant follicle and corpus luteum dynamics.   Blood plasma was collected daily to determine progesterone levels using EIA technique.   Follicular development dynamics and plasma progesterone levels were analyzed statistically using analyses of variance, while correlation between plasma progesterone levels and corpus luteum size were tested using correlation analyses.   The length of estrous cycle in local Friesian cow was 21.00 + 1.00 days.   Follicular dynamics during estrous cycle indicated only had two waves of dominant follicular development.   The first wave of dominant follicle was firstly identified on day 3, reached maximum diameter (11.17 + 0.90 mm) on day 11 and identifiable until day 15.   The second dominant follicle appeared on day 11, reached maximum size on day 21 (13.17 + 0.69 mm) and underwent ovulation on the next day.   The increased diameter of dominant follicle from days 17 to 21 was linear with growth rate of 1.20 + 0.18 mm/day.   Ovulatory dominant follicle had larger size than non-ovulatory ones (P<0.05).   Corpus luteum was ultrasonically detectable from days 5 to 19 of the estrous cycle.   The maximum size of corpus luteum (11.83 + 0.75 mm) attained on day 11, and then regressed substantially till day 19.  Plasma progesterone level started to rise during the luteal phase, reached the peak level on day 12 (4.64 + 0.23 ng/ml) and then decreased steadily till the lowest level (0.42 + 0.05 ng/ml) at the time of estrus.  The plasma progesterone profile and the corpus luteum development were concomitant each other during estrous cycle of the cow.  It can be concluded that the follicular dynamics during estrous cycle in local Friesian cows only had two waves of dominant follicular development, while plasma progesterone levels manifested corpus luteum function throughout the estrous cycle. (Animal Production 10(2): 73-77 (2008) Key Words:  Follicle, corpus luteum, progesterone, estrous cycle, cow
Identification of Excretory – Secretory Sporozoite Antigens of Eimeria tenella with Monoclonal Antibody Prastowo, J; Nurcahyo, W; Kurniasih, Kurniasih; Wasito, R
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The research done specifically to identified the. Eimeria tenella excretory-secretory sporozoit antigen by using the monoclonal antibody reaction instead. Eimeria tenella excretory-secretory sporozoit antigen. The 107 sporozoits were obtained from existation of 5x107 oocysts with 25 ml tripsin and sodium taurocholat. Excretion - secretion antigen were preparated by freezing and thawing and then be isolated with ice bath 20 second, 20 meter of amplitude as the further treatment.  Determinating the excretion -secretion sporozoit protein by using BCA-test. While the visualization sporozoit protein antigen by SDS-PAGE. Monoclonal antibody were produced through hybridization between B BALB/c limphosit immunized with Eimeria tenella excretion-secretion sporozoite antigen and myeloma cells. The monoclonal antibody identification  of excretion – secretion antigen were done through western blott. The visualization of protein molecule weight of Eimeria tenella sporozoit were resulted 20 protein fraction, included 14, 16, 17, 18, 20, 24, 26, 32, 34, 36, 39, 43, 47, 56, 65, 67, 76, 87, 94, and 96 KDa. There were 12 kinds of monoclonal antibody could be produced from these proteins. While the 5 hybridoma immunoblotting resulted specific reaction by the appearances of reaction ribbon obviously, there were MABset 1 which identified protein epitop with 14.7 KDa of molecule weight,  MAbset 2 which identified protein epitop with 43 KDa of molecule weight, MAbset 3 which identified protein epitop with 42 KDa of molecule weight, MAbset 4 which identified protein epitop with 47 KDa of molecule weight and MAbset 5 which identified protein epitop with 90 KDa of molecule weight. As the conclusion, five of excretion-secretion sporozoit antigen were proper immunogen to stimulate the hospest immunity. (Animal Production 7(2): 95-100 (2005) Key Words: Sporozoit, Antigent, Eimeria tenella, Coccidiosis
The Effect of Turmeric (Curcuma Domestica Val.) Extract on the Histopathological Lesions of Lung Induced by Cigarette Smokes in Wistar Rats Rahmad, laelatul; Milah, Nida Ul; Kusumawardani, Agapietta; Herliyani, Novi; Sarwendah, Kurniati; Sutrisno, Bambang; Wuryastuti, Hastari; Wasito, R
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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This present study is aimed to observe and determine the effects of turmeric (Curcuma domestica Val.) extract administration towards the cytotoxicity effect of cigarette smokes in the lungs of Wistar rats. Twenty Wistar rats, male, 3 months old, were used and divided randomly into four groups, of five each. Group I is as a control (K I), Group II was given 9 cigarettes/day without turmeric extract administration (K II), Group III giventurmeric extract 1 ml 0.25%/day (K III) and Group IV was given 9 cigarettes/day and turmeric extract 0.25%/day (K IV) for 60 days. At the end of the present study, all rats were necropsied and organs, particularly the lungs were collected, processed histopatologically and stained routenely with hematoxilyn eosin. The results indicated that the lungs had macrophages infiltration in the septal alveoli and hyperplasia of bronchial associated lymphoidtissue (K II). Whereas, the lungs in the rats in Groups K I, K III and K IV were still within the normal limits. It is concluded, that turmeric extract may have a potential antipulmonary cytotoxity effect due to cigarette smoke.
The Role of Extrinsic Pathway (Death Receptor Pathway) Apoptosis through Caspase-8 in Atherogenesis due to High Fat and High Cholesterol Diet Yanuartono, Yanuartono; Wuryastuti, Hastari; Wasito, R; Raharjo, Sri
Indonesian Food and Nutrition Progress Vol 12, No 1 (2005)
Publisher : Indonesian Association of Food Technologists

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Thirty male rats, strain Sprague Dawley were used as experimental animal to study the role of death receptor pathway apoptosis in atherogenesis due to high fat and high cholesterol diet. The rats were randomly alloted into three group (I, II, III) of 10 each. Group I as control was fed normal diet, group II was fed diet containing high fat diet, and group III was fed containing high fat and high cholesterol diet (atherogenic diet). After 6 and 12 weeks on experimental diet, 15 rats were selected randomly (5 rats of each group). All animal were then killed and the aorta were taken out for caspase-8 immunohistochemical analysis. Based on the present study result it can be concluded that high cholesterol and/or high fat diet induced apoptosis through death receptor pathway via caspase-8.
Diagnostic Value of Lipoprotein (a) in Cardiovascular Yanuartono, Yanuartono; Wuryastuti, Hastari; Wasito, R
Indonesian Food and Nutrition Progress Vol 5, No 2 (1998)
Publisher : Indonesian Association of Food Technologists

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This research was conducted to evaluate cardiovascular disease caused by atherogenic diets, based on immunological assay by measuring the concentration of lipoprotein (a)/Lp (a). Eighty male Sprague Dawley rats, 150-200 grams of body weight and three months of age were used in this research. The rats were randomly alloted into four groups, 20 of each. Group I as control was fed normal diet, group 11 was fed diet containing high cholesterol, group 111 was fed diet containing high fat and group 117 was fed diet containing high cholesterol and high fat (atherogenic). After 2, 4, 8, 16 weeks on experimental diet, 20 rats were selected randomly (5 rats of each group), and blood sample were withdrawn for Lp (a) analysis. All animal were then killed and the heart were taken out for histopathological analysis. The statistical analysis for Lp (a), data showed that there were significant differences (p<0.05) among all of the treatments, high fat diet had the greatest influence on Lp (a) concentration. It can be concluded that Lp (aconcentration could be influence by high fat diet, but not by period of treatment. Lp (a) concentration seems connected with the incidence of atherosclerosis in rats. For this reason, evaluation of Lp (a) concentration could be considered as a routine procedure in general health evaluation.