Sutiastuti Wahyuwardani
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Gumboro Disease: Etiology, Epidemiology, Pathology, Diagnosis And Disease Control Wahyuwardani, Sutiastuti; Agungpriyono, D.R.; Parede, L.; Manalu, W.
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 3 (2011): SEPTEMBER 2011
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Original Source | Check in Google Scholar | Full PDF (261.355 KB)

Abstract

Infectious bursal disease (IBD) or known as Gumboro, is a disease that attacks chicken older than 3 weeks, caused by famili Birnaviridae virus. Gumboro in Indonesia was firstly reported in 1983 and until now is commonly found. Very virulent IBD virus causes high morbidity and mortality that can even reach 100%. Clinical symptoms are exhibited as sluggish chicken, dropped wings and cloacal pasting. At gross examination, the bursa was found swollen, with yellowish fluid or hemorrhagic 3 days after infection. The bursa will get atrophy from 7 days post-infection. Meanwhile, the non virulent IBD virus causes subclinical symptoms. Chicken that survived, became stunted or dwarfed. On gross and histopathological findings, the bursa Fabricius has mild lesion and will recover at 14 days post-infection. Diagnosis of IBD can be determined based on pathological observation, supported by immunohistochemical examination and laboratory confirmation of disease by agar gel immunodiffusion, polymerase chain reaction techniques, antigen capture enzyme linked immunosorbent assay and isolation. Detection of antibodies can be made by serum neutralization technique or enzyme linked immunosorbent assay. Prevention can be done by routine vaccination in the field when the maternal antibodies have declined. The review describes the etiology, epidemiology, pathogenesis clinical symptoms, pathological discription and control of the disease to improve the knowledge of poultry farmer or people who are interested in poultry health. Key words: Gumboro, etiology, epidemiology, pathology, diagnosis
The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Wahyuwardani, Sutiastuti; Huminto, Hernomoadi; Parede, Lies
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Original Source | Check in Google Scholar | Full PDF (337.56 KB)

Abstract

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation.     Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption
The influence of body development of adult female Boophilus microplus ticks on their eggs fertility Wahyuwardani, Sutiastuti
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 1 (1995)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (578.403 KB)

Abstract

One hundred and seventy six adult female cattle ticks, Boophilus microplus, were collected to observed the influence of their body development on their eggs fertility under laboratory conditions (22°-32°C and 84-92% relative humidity) . The regression analysis result shown that as the body developed, eggs production and eggs fertility increased, but there was no correlation between ticks weight and the incubation periode . The relation between ticks weight and eggs production was shown as Y = -309 .433 + 13 .868X and r = 98 .5 % whereas the influence of ticks body development on eggs fertility was shown as Y = 8 .224 + 0 .338X, r = 92 .0% and the incubation periode was about 21-26 days . The fertile eggs begin to be produced by ticks with body weight of about 35-49 mg and fertility 28.39%. Key words: Cattle ticks, Boophilus microplus, eggs production, eggs fertility
Infection of Chicken Anaemia Virus: Etiology, Epidemiology, Clinical Sign, Pathological Changes and Disease Control Wahyuwardani, Sutiastuti; Syafriati, Tatty
Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 3 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (956.851 KB)

Abstract

Initially, Chicken anaemia virus (CAV) is known as CAA, which was first isolated by Yuasa in Japan in 1976. CAV particles are non enveloped with a diameter of 19 .1-20 .7nm, belonging to the family Circoviridae, genus Gyrovirus . CAV infection was first appeared in Indonesia at the same time as the outbreak of stunting and runting syndrome in 1996 with a mortality rate of 5-15% but it may reach to 60%. CAV can be transmitted vertically or horizontally. Chicken all ages is susceptible to infection. Infection of CAV occurred in young chicken flock at 2-3 weeks growth of age, causing clinical signs while in old chicken flock which is sub clinical. The signs of infectious of CAV are retarded, anaemia, anorexia, pale of face and pial. The pathology anatomy changes are pale carcases, yellowish bone marrow, atrophy of thymus and bursa fabricius . Whereas, histophatological changes are thymic necrosis of cortex and medulla, lymphocyte depletion of thymus, bursa fabricius and bone marrow. Diagnose of CAV is based on pathological changes and followed by the isolation of certain lymphoblastoid chicken cell MDCC-MSB I and then, is identified by virus neutralization. The presence of virus can also be identified by immunofluorescent and immunoperoxidase staining, in situ hybridization technique or PCR. For antibody detection, ELISA technique can be used. The syndromes of CAV infection are closely associated with those of osteopetrosis, reovirus, infectious bursal disease (IBD) and Marek. Vaccination programme in breeding farm is needed to induce maternal antibody. This paper describes the CAV disease and its occurrence in Indonesia. Key word : Chicken anaemia virus, diagnose, pathology anatomy, histopathology, control
Gumboro Disease: Etiology, Epidemiology, Pathology, Diagnosis And Disease Control Wahyuwardani, Sutiastuti; Agungpriyono, D.R.; Parede, L.; Manalu, W.
Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (261.355 KB)

Abstract

Infectious bursal disease (IBD) or known as Gumboro, is a disease that attacks chicken older than 3 weeks, caused by famili Birnaviridae virus. Gumboro in Indonesia was firstly reported in 1983 and until now is commonly found. Very virulent IBD virus causes high morbidity and mortality that can even reach 100%. Clinical symptoms are exhibited as sluggish chicken, dropped wings and cloacal pasting. At gross examination, the bursa was found swollen, with yellowish fluid or hemorrhagic 3 days after infection. The bursa will get atrophy from 7 days post-infection. Meanwhile, the non virulent IBD virus causes subclinical symptoms. Chicken that survived, became stunted or dwarfed. On gross and histopathological findings, the bursa Fabricius has mild lesion and will recover at 14 days post-infection. Diagnosis of IBD can be determined based on pathological observation, supported by immunohistochemical examination and laboratory confirmation of disease by agar gel immunodiffusion, polymerase chain reaction techniques, antigen capture enzyme linked immunosorbent assay and isolation. Detection of antibodies can be made by serum neutralization technique or enzyme linked immunosorbent assay. Prevention can be done by routine vaccination in the field when the maternal antibodies have declined. The review describes the etiology, epidemiology, pathogenesis clinical symptoms, pathological discription and control of the disease to improve the knowledge of poultry farmer or people who are interested in poultry health. Key words: Gumboro, etiology, epidemiology, pathology, diagnosis
Sensitivity of some local isolates of Mycoplasma gallisepticum against antibiotics Wahyuwardani, Sutiastuti; ., Soeripto
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

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Abstract

Sensitivity of five local isolates ofMycoplasma gallisepticum (MG) strain and two standard MG isolates obtained from Australia were tested against antibiotics of oxytetracycline, doxycycline, erythromycin, bacitracin, vancomycin, methicillin and penicillin using antibiogram disc method. The result showed that one, 2 and 3 local MG isolates were resistent to doxycycline, erythromycin and oxytetracycline respectively . MG isolate of ADA7 from Australia was found to be resistent to all antibiotics tested. None ofthe local MG isolates were sensitive against bacitracin, vancomycin, methicillin and penicillin.   Key words: Mycoplasma gallisepticum, antibiogram disc
The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Wahyuwardani, Sutiastuti; Huminto, Hernomoadi; Parede, Lies
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 1 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (337.56 KB)

Abstract

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation.     Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption
The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Wahyuwardani, Sutiastuti; Huminto, Hernomoadi; Parede, Lies
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (337.56 KB)

Abstract

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation.     Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption
The influence of body development of adult female Boophilus microplus ticks on their eggs fertility Wahyuwardani, Sutiastuti
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 1 (1995)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (578.403 KB)

Abstract

One hundred and seventy six adult female cattle ticks, Boophilus microplus, were collected to observed the influence of their body development on their eggs fertility under laboratory conditions (22°-32°C and 84-92% relative humidity) . The regression analysis result shown that as the body developed, eggs production and eggs fertility increased, but there was no correlation between ticks weight and the incubation periode . The relation between ticks weight and eggs production was shown as Y = -309 .433 + 13 .868X and r = 98 .5 % whereas the influence of ticks body development on eggs fertility was shown as Y = 8 .224 + 0 .338X, r = 92 .0% and the incubation periode was about 21-26 days . The fertile eggs begin to be produced by ticks with body weight of about 35-49 mg and fertility 28.39%.
Infection of Chicken Anaemia Virus: Etiology, Epidemiology, Clinical Sign, Pathological Changes and Disease Control Wahyuwardani, Sutiastuti; Syafriati, Tatty
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Original Source | Check in Google Scholar | Full PDF (956.851 KB)

Abstract

Initially, Chicken anaemia virus (CAV) is known as CAA, which was first isolated by Yuasa in Japan in 1976. CAV particles are non enveloped with a diameter of 19 .1-20 .7nm, belonging to the family Circoviridae, genus Gyrovirus . CAV infection was first appeared in Indonesia at the same time as the outbreak of stunting and runting syndrome in 1996 with a mortality rate of 5-15% but it may reach to 60%. CAV can be transmitted vertically or horizontally. Chicken all ages is susceptible to infection. Infection of CAV occurred in young chicken flock at 2-3 weeks growth of age, causing clinical signs while in old chicken flock which is sub clinical. The signs of infectious of CAV are retarded, anaemia, anorexia, pale of face and pial. The pathology anatomy changes are pale carcases, yellowish bone marrow, atrophy of thymus and bursa fabricius . Whereas, histophatological changes are thymic necrosis of cortex and medulla, lymphocyte depletion of thymus, bursa fabricius and bone marrow. Diagnose of CAV is based on pathological changes and followed by the isolation of certain lymphoblastoid chicken cell MDCC-MSB I and then, is identified by virus neutralization. The presence of virus can also be identified by immunofluorescent and immunoperoxidase staining, in situ hybridization technique or PCR. For antibody detection, ELISA technique can be used. The syndromes of CAV infection are closely associated with those of osteopetrosis, reovirus, infectious bursal disease (IBD) and Marek. Vaccination programme in breeding farm is needed to induce maternal antibody. This paper describes the CAV disease and its occurrence in Indonesia. Key word : Chicken anaemia virus, diagnose, pathology anatomy, histopathology, control