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Journal : Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal

Efek Antimitosis Biomutagen dari Tanaman Kembang Sungsang (Gloriosa superba L.) pada Pembelahan Sel Ujung Akar Kecambah Cabai Merah Besar (Capsicum annuum L.)

Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Flame lily (Gloriosa superba L.)  is one of the plants containing colchicine, often used as antimitotic compound, in the whole organ. This study aims to gain the combination of extract source  and extract concentration of G. superba capable of inhibit mitosis in root tip cells of chili  sprouts.  The plant is extracted  using the extraction and dilution methods to determine the extract concentration of the treatment. The squash method is used to make a  mitosis preparation. The study is arranged in a factorial using Randomized Complete Block Design (RCBD) with 4 replications. The first factor is extract source:   roots, stems, and leaves. The second factor is the extract concentration: 0, 20.40, 60, and 80%.   The observation shows that the extract of leaf can inhibit mitosis more than exctract of bulbs and roots.  All of the concentration treatment from all extract sources tend to result in similar mitotic index, but significantly different from cells that are not given treatment. The combination of the source extract and concentration of the extract result in varied mitotic index, but  it is obvious that the leaf extract with a concentration of 80% gives the most inhibiting mitosis index: 1.298%.

Co-Authors . Indarto ., Yusetyowati Abdullah, Teuku Aceng Komarudin Mutaqin Achmad subhi Gumilar Adyatma, Rengga Agus Yodi Gunawan Ahmad Sofwan Ahmad Yusron, Ahmad Akriono, Muhammad Luqman Andries Lionardo, Andries Ani Rusilowati Anis Khiyaroh, Anis Arca Datam Sugiarto, Arca Datam Arif Budi Satrio Ash Shiddiq, Dimas Ghufron Ash-Shiddiq, Dimas Ghufron Askin, Askin Askin, Askin Astuti, Desi Widi Aulanni’am a Aziza, Siti Nur Budi Prayitno BUDI SETIADI DARYONO Cahyono Purbomartono DAHRI, IRSYAD Denny Irbianto Devi, Mike Indriana Dini Siswani Mulia Djumadi Djumadi Edijatno Edijatno Eka Haryani, Eka Eko Prasetyo Eko Pudjadi, Eko Elida Novita Endang Semiarti Erly Anna Rosita Ernawiati, Eti Ervi Rachma Dewi, Ervi Rachma F. Parasmawati Firman Fuad Wirakusumah Fitri, Susi Gofar Ismail Hamid Ahmad Hardiyanto Hardiyanto Hari Wijayanto Harsi D. Kusumaningrum Heri Nugroho Hery Maryanto, Hery Husaini . Husin, Farid I Wayan Suteja Ikhwanudin Ikhwanudin Indarto - Indarto Indarto Indriani . Ishak Abdulhak Istanto Istanto Istianah Istianah Jang, Soenghoe JB. Suparyatmo Suparyatmo, JB. Suparyatmo Jenny Ratna Suminar Joko Suryanto Joko Tri Sujiwo Khairunnissa, Aulia Khoiriyah, Naila Ulfa Komalasari, Gantina Lalu A. Zaenuri Latipah, Latipah Lawrie, Muhammad Dylan Lepong, Piter Lepong, Piter lina marlina, lina LUKMAN ILHAM, LUKMAN Lutfi Aris Sasongko M Nur Ihsan Malihatin Munawaroh, Malihatin Martha Lulus Lande, Martha Lulus Masyhudi, Lalu Mateus Sakundarno Adi, Mateus Sakundarno Moh. Djazari Muhamad Afandi, Muhamad Muhammad Ade Salim Muhardjo Pudjojono, Muhardjo Muslim, Khaerul Nadjadji Anwar Nafrida Ulfah, Nafrida Nanang Sugiarto Nanda Arista Rizki Nasir Saleh Nathan Hindarto Nieke Karnaningroem Nina Fitriyati Ningtias, Rahayu Ninik Hasanah, Ninik Nuada, I Wayan Nugraha E. Suyatma, Nugraha E. Nugroho Arifianto Nur Ducha Nur Hidayah, Afifah Nurjazuli Nurjazuli NURTITI SUNUSI Pradana, Hendra Andiananta Pramudya Annisa Firdausi Pratiwi Trisunuwati Puji Wahyumi, Puji Rahmadiyanti, Ridhani Ratna P, Kenah Renan Subantoro Rini Rita T. Marpaung Risma Sake, Risma Rita Irma, Rita Rossi Prabowo Royaningsih, Nanik RUBEN DHARMAWAN Saefudin Saefudin Sahat, Afro Aransa Sasono Wibowo Satrijo Budiwibowo, Satrijo Setiawan Setiawan Setyono, Bagus Dwi Hari Shinta Arum Wahyuningsih, Shinta Arum Shofia Nur Awami Sitti Raodhah, Sitti Soekarsono Hardjono, Soekarsono Sri Hartini Sri Heny Wahyuningsih, Sri Heny Sri Suparwitri, Sri St. A. Rahayuningsih Sucipto Sucipto Sudaryati ., Sudaryati Suhardjo Widodo Dan, Suhardjo Widodo Suhartono Suhartono Suharyo Hadisaputro Sukadi, Desi Rahmawati Sukarti Moeljopawiro Suprapti Supardi Suprati Supardi Supriyanto Supriyanto Suroso . Susiarno, Hadi Sutawanir Darwis Sutiman B. Sumitro Sutrisno sutrisno Suyadi Suyadi Suyitno Suyitno Suyoto Suyoto Syahrul Basri Syaripuddin Syaripuddin Tino Bachtiar, Tino TITIK SUNDARI Tri Jalmo Trinil Susilawati Utami Dyah Syafitri Utami Kurniawati Wildana Latif Mahmudi, Wildana Latif Yanti Setianti Yudhatama, Tri Galih Yudi Widodo Yulianto Nugroho Yulianty Yulianty Yulinah S, Elin Yuwono Yuwono Zulkifli Zulkifli