A.E.T.H. Wahyuni
Faculty of Veterinary Medicine, Gadjah Mada University, Jl. Fauna No. 2, Bulaksumur Yogyakarta 55281

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Journal : ILMU KELAUTAN: Indonesian Journal of Marine Sciences

Isolasi Bakteri Asam Laktat dari Usus Ikan Kerapu Macan (Epinephelus fuscoguttatus) dan Potensinya Sebagai Antivibrio Nursyirwani, Nursyirwani; Asmara, W.; Wahyuni, A.E.T.H.; Triyanto, Triyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Tujuan penelitian ini adalah untuk mengisolasi dan menguji potensi antibakterial bakteri asam laktat dari ikan Kerapu Macan (Epinephelus fuscoguttatus) terhadap bakteri patogen Vibrio alginolyticus. Bakteri tersebut diisolasi dari usus ikan dengan metode sebarulas pada media agar MRS dan GYP+CaCO3. Koloni yang tumbuh diidentifikasi  berdasarkan  karakter  morfologi,  biokimia  dan  fisiologi.  Aktivitas  antibacterial  terhadap  V. alginolyticus diuji dengan metode difusi kertas cakram pada media Zobell 2216E lapis ganda, diameter zona jernih disekitar kertas cakram diukur dengan kaliper. Pada medium agar GYP+CaCO3 didapatkan 21 isolat yang menampakkan zona jernih disekitar koloninya. Dari karakteristik morfologi, biokimia dan fisiologi dari isolat-isolat tersebut semua isolat dapat dikategorikan kedalam genus Lactobacillus. Tetapi, dari uji aktivitas antivibrio hanya 20 isolat menunjukkan zona hambat yang berkisar dari 14,0-21,5 mm, dimana tiga isolat menunjukkan aktivitas tertinggi, yaitu berturut-turut isolat KSBU 12C, KSBU 13D dan KSBU 5Da.Kata kunci: Isolasi, bakteri asam laktat, antivibrio, Kerapu Macan The objectives of this research were to isolate and examine antibacterial potency of lactic acid bacteria from Tiger Grouper (Epinephelus fuscoguttatus) against pathogen, Vibrio alginolyticus. The bacteria were isolated from the fish intestine by spread plate method on MRS and GYP+CaCO3  agar media. The grown colonies were identified based on morphological, biochemical and physiological characters. Antibacterial activity against V. alginolyticus was examined by the paper disc diffusion method on Zobell 2216E double layer agar, and clear zone diameter around the paper disc was measured by using caliper. Twenty one isolates  with  clear zone around the colonies were obtained from the GYP+CaCO3 agar. Morphological, biochemical and physiological characteristics of the colonies and cells indicated that all isolates might be categorized into Lactobacillus. However, there were only twenty isolates showed inhibition zones from 14.0-21.5 mm in antivibrio activity test, of which the highest activity was indicated by three isolates namely KSBU12C, KSBU 13D and KSBU 5Da, respectively. Key words: Isolation, lactic acid bacteria, antivibrio, Tiger grouper.
Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) Nursyirwani, Nursyirwani; Asmara, W.; Wahyuni, A.E.T.H.; Triyanto, Triyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 3 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

  Tujuan penelitian ini adalah untuk menyeleksi isolat bakteri asam laktat (BAL) yang telah diisolasi dari ikan Kerapu Macan (Epinephelus fuscoguttatus) berdasarkan toleransi terhadap pH dan bile salts, patogenisitas dan kemampuannya menekan pertumbuhan Vibrio alginolyticus pada uji ko-kultur. Hasil penelitian menunjukkan bahwa dua puluh isolat BAL yang telah diuji antagonismenya terhadap V. alginolyticus pada penelitian terdahulu, dapat hidup pada pH rendah 3 dan tumbuh pada konsentrasi bile salts 0,5% kecuali isolat KSBU 13D.  Semua isolat tidak patogen terhadap ikan Kerapu Macan setelah diinfeksi BAL pada konsentrasi 107 cfu/ml secara intraperitoneal (IP). Pada uji ko-kultur, dari 6 isolat BAL yang dipilih, tiga isolat (KSBU 12C, KSBU 5Da, KSBU 9) berturut-turut mampu menekan pertumbuhan V. alginolyticus, dan penambahan konsentrasi BAL terbaik adalah 107 cfu/ml. Kata kunci: bakteri asam laktat, vibriosis,  patogenisitas, ko-kultur, kerapu macan The research objective was to select lactic acid bacteria which previously isolated from Tiger Grouper (Epinephelus fuscoguttatus) based on pH and bile salts tolerances, pathogenicity test and ability to decrease the growth of Vibrio alginolyticus in co-culture test. The result shows that twenty isolates which had also been examined their antagonisms against V. alginolyticus previously, were able to survive relatively low pH  values 3 and able to survive at high bile salts concentrations 0.5% except KSBU 13D isolate.  All isolates were nonpathogenic to Tiger Grouper after being intraperitoneally infected with the lactic acid bacteria at concentration 107 cfu/ml. In co-culture test, V. alginolyticus was reduced significantly by three isolates (KSBU 12C, KSBU 5Da, KSBU 9) from 6 isolates selected, and the best additional bacterial concentration was  107 cfu/ml. Key words: lactic acid bacteria,  vibriosis,  pathogenicity,  kerapu macan