A.E.T.H. Wahyuni
Faculty of Veterinary Medicine, Gadjah Mada University, Jl. Fauna No. 2, Bulaksumur Yogyakarta 55281

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Isolasi Bakteri Asam Laktat dari Usus Ikan Kerapu Macan (Epinephelus fuscoguttatus) dan Potensinya Sebagai Antivibrio

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Tujuan penelitian ini adalah untuk mengisolasi dan menguji potensi antibakterial bakteri asam laktat dari ikan Kerapu Macan (Epinephelus fuscoguttatus) terhadap bakteri patogen Vibrio alginolyticus. Bakteri tersebut diisolasi dari usus ikan dengan metode sebarulas pada media agar MRS dan GYP+CaCO3. Koloni yang tumbuh diidentifikasi  berdasarkan  karakter  morfologi,  biokimia  dan  fisiologi.  Aktivitas  antibacterial  terhadap  V. alginolyticus diuji dengan metode difusi kertas cakram pada media Zobell 2216E lapis ganda, diameter zona jernih disekitar kertas cakram diukur dengan kaliper. Pada medium agar GYP+CaCO3 didapatkan 21 isolat yang menampakkan zona jernih disekitar koloninya. Dari karakteristik morfologi, biokimia dan fisiologi dari isolat-isolat tersebut semua isolat dapat dikategorikan kedalam genus Lactobacillus. Tetapi, dari uji aktivitas antivibrio hanya 20 isolat menunjukkan zona hambat yang berkisar dari 14,0-21,5 mm, dimana tiga isolat menunjukkan aktivitas tertinggi, yaitu berturut-turut isolat KSBU 12C, KSBU 13D dan KSBU 5Da.Kata kunci: Isolasi, bakteri asam laktat, antivibrio, Kerapu Macan The objectives of this research were to isolate and examine antibacterial potency of lactic acid bacteria from Tiger Grouper (Epinephelus fuscoguttatus) against pathogen, Vibrio alginolyticus. The bacteria were isolated from the fish intestine by spread plate method on MRS and GYP+CaCO3  agar media. The grown colonies were identified based on morphological, biochemical and physiological characters. Antibacterial activity against V. alginolyticus was examined by the paper disc diffusion method on Zobell 2216E double layer agar, and clear zone diameter around the paper disc was measured by using caliper. Twenty one isolates  with  clear zone around the colonies were obtained from the GYP+CaCO3 agar. Morphological, biochemical and physiological characteristics of the colonies and cells indicated that all isolates might be categorized into Lactobacillus. However, there were only twenty isolates showed inhibition zones from 14.0-21.5 mm in antivibrio activity test, of which the highest activity was indicated by three isolates namely KSBU12C, KSBU 13D and KSBU 5Da, respectively. Key words: Isolation, lactic acid bacteria, antivibrio, Tiger grouper.

Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus)

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 3 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

  Tujuan penelitian ini adalah untuk menyeleksi isolat bakteri asam laktat (BAL) yang telah diisolasi dari ikan Kerapu Macan (Epinephelus fuscoguttatus) berdasarkan toleransi terhadap pH dan bile salts, patogenisitas dan kemampuannya menekan pertumbuhan Vibrio alginolyticus pada uji ko-kultur. Hasil penelitian menunjukkan bahwa dua puluh isolat BAL yang telah diuji antagonismenya terhadap V. alginolyticus pada penelitian terdahulu, dapat hidup pada pH rendah 3 dan tumbuh pada konsentrasi bile salts 0,5% kecuali isolat KSBU 13D.  Semua isolat tidak patogen terhadap ikan Kerapu Macan setelah diinfeksi BAL pada konsentrasi 107 cfu/ml secara intraperitoneal (IP). Pada uji ko-kultur, dari 6 isolat BAL yang dipilih, tiga isolat (KSBU 12C, KSBU 5Da, KSBU 9) berturut-turut mampu menekan pertumbuhan V. alginolyticus, dan penambahan konsentrasi BAL terbaik adalah 107 cfu/ml. Kata kunci: bakteri asam laktat, vibriosis,  patogenisitas, ko-kultur, kerapu macan The research objective was to select lactic acid bacteria which previously isolated from Tiger Grouper (Epinephelus fuscoguttatus) based on pH and bile salts tolerances, pathogenicity test and ability to decrease the growth of Vibrio alginolyticus in co-culture test. The result shows that twenty isolates which had also been examined their antagonisms against V. alginolyticus previously, were able to survive relatively low pH  values 3 and able to survive at high bile salts concentrations 0.5% except KSBU 13D isolate.  All isolates were nonpathogenic to Tiger Grouper after being intraperitoneally infected with the lactic acid bacteria at concentration 107 cfu/ml. In co-culture test, V. alginolyticus was reduced significantly by three isolates (KSBU 12C, KSBU 5Da, KSBU 9) from 6 isolates selected, and the best additional bacterial concentration was  107 cfu/ml. Key words: lactic acid bacteria,  vibriosis,  pathogenicity,  kerapu macan

ISOLATION AND IDENTIFICATION BACTERIA OF CLINICAL MASTITIS ON ETTAWAH CROSSBRED GOAT

Jurnal Sain Veteriner Vol 31, No 1 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract

ABSTACT Ettawah crossbred goat (PE) IS much more usefull to milk goat produce. One of constraints in milk goat is clinical mastitis. Clinical mastitis is economic inflict a financial lost because stopped of milk production, high cost of treatment, and even cause death. The aim of the present study was to determine the kind bacteria that can cause clinical mastitis in PE. A total of 13 clinical mastitis milk and water used to wash the six udder samples were collected from dairy PE farms. All samples were subjected  for isolation and identification of bacteria based on biochemical reactions. From the clinical mastitis milk samples, bacteria isolated were Staphylococcus aureus (55.55%), Pseudomonas sp (27.77%), Streptococcus sp (8.3%) and Bacillus sp (8.33%). Whereas, Pseudomonas sp (77.77%) and Bacillus sp (22.23%) were isolated from the water used to wash the udder. The study showed that more clinical mastitis in PE caused by Staphylococcus aureus. Water use to wash the udder was source of Pseudomonas sp causing clinical mastitis in PE.  

The Detection of Encoding Gene of Toxic Shock Syndrome Toxin-1 S. aureus Isolate from the Milk of Cows and Goats by Polymerase Chain Reaction

Jurnal Sain Veteriner Vol 31, No 2 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Abstract Staphylococcus aureus is the main bacterium found in cows and goats milk. The bacteria can produce toxin called toxic shock syndrome toxin-1 (TSST-1) that can infect humans and animals causing several serious diseases. The objective of this study was to detect the existence of encoding gene of TSST-1 S. aureus isolate from cows and goats milk. The research is initiated by re-identification stage of S. aureus conventionally continued by identification based on the molecular method of polymerase chain reaction (PCR). A number of 10 S. aureus isolates from cows and goats milk cultured in aerobics continued by Gram stain, catalase, coagulase, MSA, VJA and VP tests. The identification of S. aureus based on the molecular approach conducted by 16S rRNA gene amplification continued with amplification of TSST-1 encoding gene as the target gene. The PCR product of TSST-1 encoding gene then sequenced to ensure whether the DNA fragment amplified is the TSST-1 encoding gene or not. The result of the research indicates that re-identification of S. aureus conventionally generating positive reaction of S. aureus species. Molecular identification of 16S rRNA gene amplification gives a good result by producing DNA fragment of 745 bp size and meets the target gene. The detection result of encoding gene TSST-1 gives negative result marked by DNA fragment which the size does not match the target gene. The allignment result of sequence isolate SA.1 indicates that the sequence is not tst gene but the gene which coded glutamate sinthetase belongs to S. aureus, whereas sequence of isolate KI.8 is 50S rRNA gene belongs toS. saprophyticus.

Isolasi and Identification of Bacteria from the Urine Fluid Organic Fertilizer (POC)Ettawa Crossbred (PE) in The Sleman Regency

Jurnal Sain Veteriner Vol 31, No 2 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Urine is a by product from Ettawa crossbred (PE) goat farm in Sleman regency. In addition, the manure urine goats can be used for fluid organic fertilizer (POC). Recently, organic farming is more interesting for people because it does not use any chemical fertilizers. Salmonella sp and E. coli O157:H7 are pathogenic bacteria that can contaminate agricultural products and dangerous for public health. One of sources of contamination in agricultural products is due to the organic fertilizer. Therefore, the aim of the present study was to determine pathogenic bacteria from POC urine PE goats in Sleman regency. A total of 8 POC samples were collected from PE goat urine in Sleman regency. These samples were analyzed for Salmonella sp, E. coli O157:H7 and other pathogenic bacteria by using biochemical reactions. All samples are negative for Salmonella sp. and E. coli O157:H7 as well. Whereas, 85% of 8 samples are positive for Pseudomonas sp. The present study showed that POC from PE goats farm in Sleman regency are negative for pathogenic bacteria.  

HUBUNGAN ANTARA PRAKTEK MANAJEMEN PEMELIHARAAN DENGAN KEJADIAN AVIAN INFLUENZA PADA PETERNAKAN AYAM PEDAGING DI SEKTOR 3 MILIK MITRA PT. DUTA TECHNOVET DI DIY SELAMA SATU SIKLUS PEMELIHARAAN

JURNAL PETERNAKAN Vol 8, No 1 (2011): Februari 2011
Publisher : JURNAL PETERNAKAN

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Abstract

The control strategies of avian influenza (AI) inpedaging operations primarily depend on tight biosecuritymeanwhile management practices on farm. This experiment was designed to study the relationship between the level ofbiosecurity and the incidence of AI in one rearing period ofpedaging farms at sector-3 with an opened house system. Thisstudy was done in contractpedaging company farms of PT. Duta Technovet which has a minimum of 1.000 chickens. Thisstudy was conducted in 18 houses (n) ofpedaging farms which were distributed in 3 different villages of 3 differentsubdistricts and districts in Yogyakarta Special Region. Sixpedaging houses were selected from Sendang Sari Village ofPajangan subdistrict of the district of Bantul; 6 pedaging houses were from Getas Village of Playen subdistrict of the district of Gunungkidul; and 6pedaging houses were from Pakem village of Pakem subdistrict of the district of Sleman. The judgment based on the score of the prepared questionnaire for thepedaging farmers. Isolation of AI virus employing cloacal swab was done in the Virology Laboratory of Balai Besar Veteriner (BBVet) Wates. Data were collected and analyzed with the Fishers exact test (FET), using the software SPSS of Windows version 15.0. The results of this study indicate that the presence of stable air ventilation sufficient to give significant results on the incidence of AI disease inpedagings, with P <0.01.

VIRULANCE FACTOR OF Staphylococcus sp. ISOLATED FROM SUBCLINICAL MASTITIS IN ETTAWA GRADE GOAT’S MILK IN SLEMAN REGENCY -YOGYAKARTA

Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

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Abstract

Stapphylococcus sp., is bacteria that caused subclinical mastitis in Ettawa Grade (EG) goat. Thepurpose of this study was to determine virulance factor Stapphylococcus sp., which was isolated fromsubclinical mastitis EG goat’s milk in Sleman regency, Yogyakarta. A total of 7 isolate Stapphylococcussp., were isolated from subclinical mastitis EG goat’s milk were determinated by several virulancefactors such as haemolysin, clumping factor, and coagulase. Haemolysin was determinated by culture inblood agar plate and incubated in the temperature of 37°C for 24 hours. Clumping factor wasdeterminated by mixing the rabbit plasma with Stapphylococcus sp., in the glass objects. Coagulase wasdeterminated by mixing the rabbit plasma and broth culture of Stapphylococcus sp. After incubated inthe temperature of 37°C for 24 hours in tube, then the gel formation was observed. Haemolytic type ßwas yielded from 5 isolate Stapphylococcus sp., whereas 2 isolates were not haemolytic. Clumpingfactor and coagulase were produced from 2 isolate Stapphylococcus sp. This study showed that not all ofStapphylococcus sp., isolate causing subclinical mastitis in EG goat have virulance factor.

THE SURFACE CHARACTER OF Staphylococcus aureus ISOLATED FROM SUBCLINICAL MASTITIS OF DAIRY COW SUPPORTING ADHERENCE TO UDDER EPITHELIAL CELL

Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 3 (2010): (September)
Publisher : Diponegoro University

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Abstract

Staphylococcus aureus (S. aureus) is one among pathogen bacteria that caused mastitis in dairycow in Indonesia. Its ability to initiate infection depends on the surface character of the bacteria. Thesurface character of S. aureus is determined by the corelation of red blood cell hemagglutination activity,hydrophobicity, production of protein A, and bacterial adherence to the mammary epithelial cells of thehost. This research was designed to characterize the surface of S.aureus, which correlates to bacterialadherence. Four isolates of S. aureus were taken from subclinical mastitis milk of dairy farm in Malang,East Java. The surface character of S.aureus was determined by performing the red blood cellhemagglutination test using the dairy cow erythrocyte suspension with concentration of 0.5%, 1%, and2%. The hydrophobicity expression was determined by Salt Aggregation Test (SAT) method using theAmmonium Sulfate suspension with concentration of 1.2 M, 1.6 M, 2.0 M, 2.4M, and 3.2 M. Serum softagar (SSA) test was used to indicate the production of protein A; Ability of adhesion was done byadhering the S.aureus, which were already labelled by Fluorescent Ichtiocyanate (FITC) to themammary epithelial cell of post-calving mice. Result of this this research showed that the surfacecharacters of S.aureus, which correlates to the adherence, comprise of erythrocytes hemagglutinationability, hydrophobicity, and protein A production. Isolates of S.aureus, which is hydrophobic, possesshaemagglutinine, and protein A were able to adhere to mammary epithelial cell with higher quantity

Escherichia coli Surface Characters of Ettawah Cross Breed Goats Milk on the Adhession Ability of Mammary Epithelial Cells

Jurnal Sain Veteriner Vol 33, No 1 (2015)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Escherichia coli is a bacteria that may cause mastitis in goats. The ability of bacteria to infect is influenced by surface characters of each bacterial cell. Adhesion of bacteria on the udder epithelial cells plays an important role in the incidence of subclinical mastitis.The purpose of this study was to determine the surface characters of Escherichia coli colony morphology, such as the presence of polysaccharides capsule, hemagglutination activityand hydrophobicity contributes to the adhesion process. Three isolates of Escherichia coli isolated from Ettawah cross breed goats on Mandiri and Pangestu Farm at Turi, Sleman, Yogyakarta were used in the study. The ability of E. coli to fermentize lactose was done by examining its ability in fermented lactose on eosin methylene blue. Hemagglutination ability was seen by hemagglutination test using sheep erythrocytes at concentrations of 0.5%, 1%, 1.5% and 2%, respectively. Hydrophobicity expression was done by saltaggregation test using ammonium sulfate ([NH4] 2SO4) at concentrations of 1.2 M, 1.6 M, 2.0 M, 2.4 M and 3.2 M, respectively. The presence or absence of sera proteins was tested with sera soft agar, and the ability of adhesion was done by adhesion of Escherichia coli which has been marked by fluorescein isothiocyanate , with rats udder epithelial cells after birth. The results showed that isolates of E. coli with dry surface characters had athin polysaccharide capsule, positive hemagglutination , and hydrophobic, which had the ability to stick more on the udder epithelial cells after birth. Meanwhile, those with mucoid surface characters, had a thick polysaccharide capsule, negative hemagglutination, and hydrophilic which had a lower adhesion ability.

ISOLATION AND IDENTIFICATION BACTERIA OF CLINICAL MASTITIS ON ETTAWAH CROSSBRED GOAT

Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Abstract

ABSTACT Ettawah crossbred goat (PE) IS much more usefull to milk goat produce. One of constraints in milk goat is clinical mastitis. Clinical mastitis is economic inflict a financial lost because stopped of milk production, high cost of treatment, and even cause death. The aim of the present study was to determine the kind bacteria that can cause clinical mastitis in PE. A total of 13 clinical mastitis milk and water used to wash the six udder samples were collected from dairy PE farms. All samples were subjected  for isolation and identification of bacteria based on biochemical reactions. From the clinical mastitis milk samples, bacteria isolated were Staphylococcus aureus (55.55%), Pseudomonas sp (27.77%), Streptococcus sp (8.3%) and Bacillus sp (8.33%). Whereas, Pseudomonas sp (77.77%) and Bacillus sp (22.23%) were isolated from the water used to wash the udder. The study showed that more clinical mastitis in PE caused by Staphylococcus aureus. Water use to wash the udder was source of Pseudomonas sp causing clinical mastitis in PE.