Agung Wahyu Susilo
Pusat Penelitian Kopi dan Kakao Indonesia

Published : 4 Documents
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Relationship Between Pod Characteristics of Some Cocoa Clones (T. cacaoL.) and Their Resistance Response to Cocoa Pod Borer Wahyu Susilo, Agung; Mangoendidjojo, Woerjono; ., Witjaksono
Pelita Perkebunan (Coffee and Cocoa Research Journal) Vol 23, No 3 (2007)
Publisher : Indonesian Coffee and Cocoa Research Institute

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Abstract

Characteristic study on CPB resistance had been carried out by characterizing mesocarp layer of cocoa pod where egg laying and larva penetration are carried out. For this study, 4 clones were selected which performed different response to CPB, namely resistant clones of KW 514 and ARDACIAR 10, moderately resistant clone of KW 411 and the susceptible clone of RCC 72. Pod samples were classified as young and mature were detached from cocoa collection at the experimental station of Kaliwining, Indonesian Coffee and Cocoa Research Institute in Jember. Characterization was conducted based on microchemical method at the Microtechnique Laboratory of Gadjah Mada University to identify trichome appearance at upper layer of pod, distributed granules of tannin through mesocarp and lignification of the sclerotic. It was characterized that the trichome density performing resistant in which the resistant and moderately resistant clones had more number of trichome density than of the susceptible one. The distributed granules of tannin at young pod showed significant difference among those clones that indicate resistant characteristics. The number of distributed granules of tannin was higher of those the resistant and moderatly resistant clones than of the susceptible clones that were formed both at furrow and at ridge of pod. However, the granules of tannin were distributed more at ridge than at furrow where furrow were preferred by CPB for egg laying. The characteristic differences between the resistant and moderately resistant clones were identified based on the intensity of lignification at sclerotic tissue. The lignified tissue of the resistant clones perform more intensive and more compact than of the moderately resistant clones. Tissue of young pod of the moderately resistant and susceptible clones was not yet lignified. Thickness of lignified tissue at the furrow of resistant clone was higher than of the moderately resistant and susceptible clones. Key words : Theobroma cocoa L.,cocoa pod borer, resistant, mesocarp, sclerotic, trichome, tannin.
Stability in Flowering, Flushing, and Fruiting Characters of 21 Potential Cacao Clones in ICCRI Collection Sari, Indah Anita; Wahyu Susilo, Agung
Pelita Perkebunan (Coffee and Cocoa Research Journal) Vol 29, No 2 (2013)
Publisher : Indonesian Coffee and Cocoa Research Institute

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Abstract

Flowering character and flowering intensity can be used to estimate productivity and it is important for supporting breeding activity especially for pollination program. This research was aimed to study the stability of character of flushing, flowering and fruiting on different cocoa clones (genetic). The research was done at Kaliwining Research Station, Indonesian Coffee and Cocoa Research Institute, Jember. There were 21 clones; KW 422, KW 426, KW 427, KW 425, KW 523, KW 062, KW 084, KW 094, KW 162, KW 163, KW 165, KW 219, KW 235, KW 236, KW 265, KW 005, KW 292, KW 570, KW 514, KW 516, and KW 617. Flowering intencity, fruiting, and flushing were observed. The result of the study showed that KW 084 was the most stable clone for fruiting. KW 235 had high pod number in April. KW 426, KW 427, KW 523, and Sulawesi 2 had high pod number in March and June. KW 219 and KW 516 had the lowest pod number. Low pod number did not occur in March until June. Flowering and flushing on all genotypes showed the stability value with IPCA value nearly zero. KW 094 showed high flowering intensity in October. High flushing intensity occurred in January and July, the lower one in April and August. Key words: Stability analysis, flushing, flowering, fruiting, cocoa clones
Development of Selection Criteria on Bean Weight Character of Cocoa (Theobroma cacaoL.) through Path Analysis Approach Anita Sari, Indah; Wahyu Susilo, Agung
Pelita Perkebunan (Coffee and Cocoa Research Journal) Vol 29, No 3 (2013)
Publisher : Indonesian Coffee and Cocoa Research Institute

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Abstract

Path coefficient analysis is frequently used for development of selection criteria on various type of plants. Path analysis on this research was conducted to find the selection criteria of yield component which directly affect bean weight. In addition to the value of path analysis coefficient, genetic variation coefficient, heritability and the value of genetic progress were also studied. The study was conducted at the Indonesian Coffee and Cocoa Research Institute. The research used randomized complete block design consisting of 14 accession numbers and each consisting of three replications. Pod girth, pod length, pod weight, wet beans weight per pod, number of normal beans per pod, number of abnormal beans per pod, dry weight per normal bean, and shell content were observed. The results showed that the pod weight character had an important role in determining the dry weight of normal bean. The character had a positive genotype correlation coefficient values which was high and significantly different (r=0.46) for dry weight per normal bean, considerable direct influence (P=0.479), moderate of the genotype variation coefficient (9.6%), and high genetic progress (95.23). Character of wet bean weight per pod could also be used indirectly for the selection criteria for dry weight per normal bean based on genetic variation coefficient value (11.88%), genetic progress value (82.48), and direct effect on dry weight per normal bean had positive value (P=0.006). Key words: Selection criteria, dry weight per bean, path analysis,Theobroma cacaoL.
Self-Compatibility Crosses of Several Cocoa Clones Wahyu Susilo, Agung
Pelita Perkebunan (Coffee and Cocoa Research Journal) Vol 22, No 3 (2006)
Publisher : Indonesian Coffee and Cocoa Research Institute

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Self compatibility cross in cocoa is a useful criterion in utilizing germplasm collection. Evaluation of self-compatibility crosses on cocoa clones has been carried out in Kaliwining Experimental Station of ICCRI by treating artificial self-pollination. The observed clones were TSH 858, ICS 60, ICS 13, UIT 1, KW 162, KW 165, KW 163, DR 1, DR 2, DRC 16, DRC 15, KKM 22, Na 32, Na 33 and DR 38. Self-compatibility crosses were identified by percentage of fruit set evaluated during 6 consecutive weeks after pollination. The results showed that the percentage of fruit set were significantly different among clones in the range of 0—46.34%. Three groups of compatibility of cocoa clones were identified i.e. first, self-incompatible clones of DR 1, Na 32, Na 33; second, partially self compatible clones of DR 38, TSH 858, ICS 60, ICS 13, UIT 1, KW 162, KW 165, KKM 22; and third, self-compatible clones of DR 2, DRC 16,DRC 15, KW 163. Clones which perform self-incompatible and partially self-compatible furthermore can be used as female parents in the production of hybrids. Key words: self-compatibility, Theobroma cacao, clones.