Endang Nur W
Faculty of Medicine Universitas Diponegoro, Semarang

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Nutrional Value and Amino Acid Composition of Tempe Gembus and Its Effect on Growth of Rats Sulchan, Mohammad; W, Endang Nur
Journal of the Indonesian Medical Association Vol. 57 No. 3 March 2007
Publisher : Journal of the Indonesian Medical Association

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Abstract

It has recently been proven that soybean “tempe” can improve the blood lipid profile inhuman as well as in animal. People in Central Java also consume another traditionally fermented food called “tempe gembus” which is made from soybean solid waste of soya-curd factory fermented by tempe mold Rhizopus sp. The aim of this study was to analyze the nutritional value, amino-acid composition and its effect of some various concentration of “tempe gembus” on growth of rats. The concentrations of tempe gembus used in this study were 0%, 4%, 8%, and12% (w/w basic). All diets were isocaloric and isoprotein based on the AIN 93M purified diet standard formula. Twenty eight male Spague Dawley rats 10 weeks old with normal body weight were housed in individual stainless-steel cage for acclimatization. Animal were then assigned toone of four dietary treatment groups by randomization (n=7). Dietary treatment (G-0, G-1, G-2 and G-3) was given for 5 weeks. At all steps of the experiments, diet and water were offered adlibitum. Body weight were recorded weekly, while food intake was recorded daily. Data collected were then analyzed using the paired t-test and oneway Anova, followed by Least Significant Difference at 95% confidence level. The result of study revealed that tempe gembus are not composed of high value energy and protein, but contains essential amino acid. The effect of three dietary treatment groups of tempe gembus are not different compared to standard dietary treatament (G-0, 0% tempe gembus).Keywords: isocaloric isoprotein, Sprague Dawley rats