Ristianto Utomo
Lab. Hijauan Makanan Ternak dan Pastura, Fakultas Peternakan, Universitas Gadjah Mada, Jl. Fauna No 3 Kampus Bulaksumur UGM, Yogyakarta

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Journal : Buletin Peternakan

Estimasi Sintesis Protein Mikrobia Rumen Menggunakan Ekskresi Derivat Purin dalam Urin dengan Teknik Spot Sampling pada Kambing Bligon dan Kambing Kejobong Putra, Dianestu; Yusiati, Lies Mira; Utomo, Ristianto
Buletin Peternakan Vol 40, No 3 (2016): BULETIN PETERNAKAN VOL. 40 (3) OKTOBER 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.095 KB) | DOI: 10.21059/buletinpeternak.v40i3.12766

Abstract

This study were aimed to determine the correlation between concentration of purine derivatives (PD) in spot sample with PD total excretion in Bligon and Kejobong goats and determine the appropriate sampling time, in order to predicting microbial protein synthesis in both breeds. Six male Bligon goats and six male Kejobong goats with age range from 8 to 14 months and body weight from 16 to 21 kg were placed in metabolism cages. Peanut straw and water were given to both groups of goats through ad libitum feeding and drinking. The study was done in 14 days for adaptation, 3 days for collection. Sample of feeds, feed residues, and feces were collected and then analyzed to determine dry matter and organic matter content. Spot urine and the total daily urine samples were also collected. Samples collection of spot sampling technique was run by taking the urine periodically with 3 hours intervals at 24 hours. Urine samples were analyzed for the content of creatinine and PD which includes allantoin, uric acid, xanthine, and hypoxanthine. Data were tested for the correlation between concentration of PD spot urine sample with total PD daily excretion. It is known that the concentration of PD and creatinine (µmol/L) for Bligon were 1,418.40 and 202.85 respectively, while for Kejobong were 1,547.40 and 219.68 respectively. Total excretion of PD, allantoin, uric acid, xanthyne and hypoxanthine and creatinine (µmol/W0,75/day) for Bligon were 114.14, 95.86, 17.31, 0.97, and 16.40 respectively, with microbial protein synthesis efficiency was 4.61 g N/kg degraded of organic matter in rumen (DOMR). Total excretion of PD allantoin, uric acid, xanthyne and hypoxanthine and creatinine (µmol/W0,75/day) for Kejobong were 180.18, 158.17, 20.60, 1.40, and 24.87 respectively, with microbial protein synthesis efficiency was 6.90 g N/kg DOMR. Based on this study also known that the best time for spot sampling to determine the total excretion of PD in Bligon was in the range time of 11.00 am to 2.00 pm, with equation Y=1.474X+48.81, while Kejobong goat in the range of 2.00 to 5.00 pm, with equation Y=2.678X+5.692.
Pengaruh Inokulasi Lactobacillus plantarum dan Saccharomyces cerevisiae terhadap Fermentasi dan Kecernaan In Vitro Silase Kulit Buah Kakao Zakariah, Muhammad Askari; Utomo, Ristianto; Bachruddin, Zaenal
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.843 KB) | DOI: 10.21059/buletinpeternak.v40i2.9294

Abstract

The objective of this study was to identify the effect of L. plantarum and S. cerevisiae mixed culture inoculation into cocoa pods silage on chemical composition and in vitro digestibility. The four treatments were: 1 kg freshly harvested cocoa pods without inoculants as control (K); K + L. plantarum (KLp); K + S. cerevisiae (KSc); and K + L. plantarum and S. cerevisiae mixture (KLp+Sc) 0.1% dry matter, Cassava meal were added in all treatments. Each treatment was replicated 3 times, and then fermented for 21 days. Parameters observed in current study were gas test production, ruminal fermentation parameter, and in vitro digestibility. The collected data were analyzed by one-way analysis of variance and followed by Duncan’s new Multiple Range Test for data with significant differences. Result showed that the mixed culture Lp+Sc inoculation increased (P<0.05) chemical quality of cocoa pods by reducing fibre fraction and increase NFE contents, increased degradation rate, degradation theory, reduced rumen pH, and propionate acid production, without affecting acetate to propionate ratio, microbial protein synthesis, and digestibility of cocoa pod silage.
THE EFFECT OF MOLASSES, Lactobacillus plantarum, Trichoderma viride, AND ITS MIXTURES ADDITION ON THE QUALITY OF TOTAL MIXED FORAGE SILAGE Chalisty, Vian Dwi; Utomo, Ristianto; Bachruddin, Zaenal
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (703.83 KB) | DOI: 10.21059/buletinpeternak.v41i4.17337

Abstract

The objective of this research was to determine the effect of molasses, Lactobacillus plantarum, Trichoderma viride, and its mixtures addition on the quality of total mixed forage silage. Molasses was added 4% (w/w), L. plantarum 0,1% (v/w), and T. viride 0,1% (v/w). Each treatment was made 3 replication and then fermented for 21 days anaerobically. Variables measured were physical, chemical, and biological quality includes color, odor, texture, fungi, pH, lactic acid content, and ammonia. Data were analyzed using the analyses of variance Completely Randomized Designs. If there was significant among the treatment, it followed by a test of the average between two treatments with Duncan’s New Multiple Range Test. Test the quality of physical showed a yellowish green color, smell sour, dense texture, and the presence of the fungus little / no fungi. The addition of molasses alone or a mixture of molasses and L. plantarum/T. viride lowered pH and ammonia (P <0.05), while the lactic acid content increased (P <0.05). The addition of molasses or water-soluble carbohydrate is a must to produce good quality total mixed forage silage that shown with pH 3.60, lactic acid content 4.28% DM, ammonia content 0.43% DM.
IMPROVING PHYSICO-CHEMICAL CHARACTERISTIC AND PALATABILITY OF KING GRASS (Pennisetum hybrid) SILAGE BY INOCULATION OF Lactobacillus plantarum - Saccharomyces cerevisiae CONSORTIA AND ADDITION OF RICE BRAN Sofyan, Ahmad; Widyastuti, Yantyati; Utomo, Ristianto; Yusiati, Lies Mira
Buletin Peternakan Vol 41, No 1 (2017): BULETIN PETERNAKAN VOL. 41 (1) FEBRUARI 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.789 KB) | DOI: 10.21059/buletinpeternak.v41i1.12980

Abstract

This study was conducted to determine effectiveness of inoculants consisted of lactic acid bacteria Lactobacillus plantarum (Lp) and yeast Saccharomyces cerevisiae (Sc) combined with addition of rice bran on the physico-chemical characteristics and palatability of king grass (Pennisetum hybrid) silage. The experiment was arranged on the factorial randomized design (3x3) consisting of the inoculants treatments (control, Lp, Lp+Sc) and the addition level of rice bran (0, 5 and 10%). The measured variables were physico-chemical characteristics i.e. colour, odour, pH, lactic acid, Fleigh points, and palatability of silage. Inoculation of Lp and Lp+Sc improved silage odour and reduced fungal contamination. Silage was treated by Lp+Sc and rice bran (5-10%) showed reduction of pH and an increase of lactic acid and Fleigh points. However, interaction between inoculants and rice bran treatment was not significance. Either inoculation or addition of rice bran tended to enhance the palatability of silage in cattle. It concluded that the addition of inoculants L. plantarum and S. cerevisiae with/without addition of 5-10% rice bran could improve the physico-chemical characteristics of silage and its palatability to ruminant.
EFFECT OF LIGNOSELULOLITIC FUNGUS TO ENZIMATIC ACTIVITY, FIBER FRCTION, AND DIGESTIBILITY ON FERMENTATION PROCESS OF COCOA POD Yakin, Engkus Ainul; Bachruddin, Zaenal; Utomo, Ristianto; Millati, Ria
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.832 KB) | DOI: 10.21059/buletinpeternak.v41i3.22657

Abstract

The study was conducted to determine the enzyme activity, fiber fraction and digestibility in the fermentation process of cocoa pod. The substrate was used the cocoa pod while the fungi used Phanerochaete chrysosporium, Pleurotus ostreatus and Schizophyllum commune. Preparation of cocoa pod was chopped, finely ground and then dried. Preparation of fungi by growing fungi in liquid medium. Research methodology was the fermentation conducted with different fungi used four treatments and five replications. T1 = fermentation of cocoa pod without fungi addition, T2 = fermentation of cocoa pod with P. chrysosporium addition, T3 = fermentation of cocoa pod with  P. ostreatus addition, and T4 = fermentation of cocoa pod with S. commune. Fermentation used Erlenmeyer 250 ml and weight of cocoa pod was 100 gram. Fungi added on 5% from substrat weight bassis dry matter. Variables observed was enzyme activity, fiber fraction and digestibility . This study was designed using research design completely randomized design with a unidirectional pattern analysis of variance. Significant variables followed Duncan’s multiple range test. The results showed fermentation used P. chrysosporium has highest lignin peroxidase enzyme activity of 0.52±0.04 U/mL and mangan peroxidase 0.06±0.00 U/mL, neutral detergent fiber 75,54±0,41%, acid detergent fiber 68,10±0,30%, lignin 26,86±0,19%, cellulose 27,17±0,25%, hemicellulose 6,77±0,52%, dry matter digestibility 69,70±0,43% and organic matter digestibility 69,59±1,03%. The conclusion from this research that the fermentation by using fungi P. chrysosporium addition has the best result to degradated lignin. 
ADDITION OF CASSAVA IN THE BASAL FEED BASED ON RICE STRAW FERMENTATION TO IMPROVE THE DIGESTIBILITY IN VITRO ON THE BALI CATTLE Christofel, John Bernhard Sendow; Noviandi, Cuk Tri; Utomo, Ristianto
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (630.058 KB) | DOI: 10.21059/buletinpeternak.v41i4.8807

Abstract

INTISARI Tujuan penelitian untuk mengetahui daya cerna sapi Bali secara in vitro terhadap penambahan ubi kayu pada waktu yang berbeda terhadap pakan basal jerami padi fermentasi. Penelitian ini selama 4 bulandan dilaksanakan di Laboratorium Teknologi Makanan Ternak, Biokimia Nutrisi, Uji Teknologi Pangan dan Hasil, Universitas Gadjah Mada, Yogyakarta. Persiapan alat dan bahan yakni sapi Bali berfistula rumen, jerami padi fermentasi, ubi kayu, seperangkat bahan dan alat laboratorium untuk analisis proksimat in vitro.Uji in vitro tahap pertama untuk mengetahui kecernaan bahan kering (BK), bahan organik (BO), konsentrasi NH3, protein mikrobia dan VFA, penelitian menggunakan metodein vitroTilley dan Terry dengan memodifikasi pada bagian tutup tabung reaksi. Data dianalisis dengan menggunakan analisis variansi completely randomized design (CRD) one-way ANOVA dengan bantuan XLSTAT. Analisis dilanjutkan menggunakan Duncan’s new multiple range test (DMRT) jika terdapat perbedaan. Hasil uji in vitro menunjukkan perbedaan nyata (P<0,05) dengan perlakuannya yaknitanpa penambahan 10% (0 jam) danpenambahan 10%ubi kayu kering pada jam ke 2, 4, dan 6sebagai berikut BK(35,35%, 46,01%, 45,27%,41,52%). BO (44,74%, 53,57%, 50,49%, 51,04%),NH3 (6,77 mg/100 ml, 4,85 mg/100 ml, 5,66 mg/100 ml, 5,39 mg/100 ml), protein mikrobia (0,17 mMol/ml/g, 0,15 mMol/ml/g, 0,14 mMol/ml/g, 0,14 mMol/ml/g), VFA : asetat, (22,98 mM, 20,18 mM, 30,70 mM, 35,21 mM), propionat (5,51 mM, 4,87 mM, 5,40 mM, 7,46 mM), butirat (1,38 mM, 2,51 mM, 3,23 mM, 3,42 mM). Kesimpulannya yakni penambahan ubi kayu pada waktu berbeda setelah diberi pakan basal jerami padi fermentasi dapat meningkatkan kecernaan sapi Bali secara in vitro.  (Kata Kunci :  In Vitro, Jerami Padi Fermentasi, Sapi Bali, Ubi Kayu).
Effect of Phosphate Fertilizer and Arbuscular Mycorrhizal Fungi on The Nutrient, Phosphateuptake and in Vitro Digestibility of Alfalfa Suwignyo, Bambang; Putra, Bela; Umami, Nafiatul; Wulandari, Cahyo; Utomo, Ristianto
Buletin Peternakan Vol 40, No 3 (2016): BULETIN PETERNAKAN VOL. 40 (3) OKTOBER 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (652.231 KB) | DOI: 10.21059/buletinpeternak.v40i3.12401

Abstract

This study aimed to determine the effect of arbuscular mycorrhizal fungi (AMF) and phosphate (P) fertilizer on the nutrient content, phosphate uptake and in vitro digestibility of alfalfa (Medicago sativa L.).The research was conducted at green house of Forage and Pastures Science Laboratory, Faculty of Animal Science Universitas Gadjah Mada. The experiment was arranged in Completely Randomized Design using 3x4 factorial patterns with four replications. The first factor was dosage of phosphate fertilizer SP 36 (0, 60, and 120 kg/ha). Second factor was the dosage of AMF (0, 0.8, 1.6, and 2.4kg/ha). The variable measured was nutrient contents (crude protein, dry matter, and organic matter), total P uptake and dry matter and organic matter in vitro digestibility. The results showed that the interaction of AMF and P fertilizer had no significant effect on crude protein and total P uptake, but highly significant effect on the parameters of dry matter, organic matter and dry matter and organic matter in vitro digestibility.