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ISOLASI DAN PEMANFAATAN BAKTERI PROTEOLITIK UNTUK MEMPERBAIKI KUALITAS LIMBAH CAIR PENGOLAHAN BANDENG PRESTO Paskandani, Riky; Ustadi, Ustadi; Husni, Amir
Jurnal Manusia dan Lingkungan (Journal of People and Environment) Vol 21, No 3 (2014)
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

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Abstract

Industri pengolahan ikan menghasilkan limbah dengan kadar protein tinggi. Penambahan bakteri proteolitik diharapkan dapat membantu mendegradasi limbah berprotein tinggi. Pada penelitian ini, isolasi bakteri proteolitik dilakukan untuk mendapatkan isolat dengan aktivitas proteolitik tinggi. Isolat terbaik diuji kemampuan hidup dan aktivitasnya pada berbagai konsentrasi NaCl dan pH. Kemampuan isolat meningkatkan kualitas limbah juga diuji pada limbah pengolahan bandeng presto. Hasil penelitian diperoleh 36 isolat bakteri proteolitik dari limbah cair pencucian ikan Pasar Kranggan Yogyakarta. Dari ke-36 isolat tersebut, isolat D61 merupakan isolat dengan aktivitas proteolitik tertinggi dengan zone diameter 20,5 mm. Isolat tersebut mampu hidup pada NaCl 0-10% dan kisaran pH 5-10, namun aktivitas proteolitik tertingginya pada NaCl 0-2% dan kisaran pH 7-8 dan pH 10. Berdasarkan karakteristik morfologi dan biokimianya, isolate D61 memiliki kemiripan 93,93% dengan Bacillus soli. Hasil ujicoba pada limbah cair pengolahan bandeng presto ternyata isolat D61 tidak mampu memperbaiki kualitas limbah tersebut.
Penggunaan Ekstrak Rumput Laut Padina sp. untuk Peningkatan Daya Simpan Filet Nila Merah yang Disimpan pada Suhu Dingin Husni, Amir; Ustadi, Ustadi; Hakim, Andi
Agritech Vol 34, No 3 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

The aim of the research was to determine effect of Padina sp. extract on shelf life of red nile fi llet during storage at low temperature. Red nile fi llet was soaked up into Padina sp. extract solution at various concentration of 0; 0.5%; 1%; 1.5%; and 2% for 30 minutes, then was stored in chilling room for 10 days. The observation was carried out every 2 days. The parameters observed were pH, Total Plate Count (TPC), Total Volatile Base-Nitrogen (TVB-N), and organoleptic still be tests. The results showed that the different concentration of Padina sp. extract yielded the signifi cant effect (P<0,05) on pH, TPC, TVB-N and organoleptic of the red nile fi llet. The treatment of Padina sp. extract on red nile fi llet can still be consumed up to 8 days of storage based on TPC, TVB, and organoleptic namely odor and texture. The treatment 1% of Padina sp. extract was the best treatment in maintaining shelf life of red nile fi llet stored at low temperature.ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak Padina sp. terhadap daya simpan fi let nila merah selama penyimpanan pada suhu dingin. Filet nila merah direndam selama 30 menit dalam larutan ekstrak Padina sp. dengan konsentrasi: kontrol; 0,5%; 1%; 1,5%; dan 2%, kemudian disimpan pada chilling room selama 10 hari dengan selang waktu pengamatan setiap 2 hari. Parameter yang diamati meliputi: pH, Total Plate Count (TPC), Total Volatile Base-Nitrogen (TVB-N), dan organoleptik. Hasil penelitian menunjukkan bahwa penggunaan ekstrak Padina sp. yang berbeda memberikan pengaruh nyata (P<0,05) terhadap nilai pH, kandungan bakteri total, kandungan TVB-N, dan nilai organoleptik keseluruhan fi let nila merah selama 10 hari penyimpanan pada suhu dingin. Filet nila merah yang diberi ekstrak Padina sp. masih layak dikonsumsi sampai hari ke-8. Penggunaan ekstrak Padina sp. 1% merupakan perlakuan terbaik dalam mempertahankan kesegaran fi let nila merah yang disimpan pada suhu dingin.
Penyiapan Starter Kering Bakteri Asam Laktat Halofilik untuk Pengolahan Hasil Perikanan Fermentatif Bergaram Ustadi, Ustadi; Suparmo, Suparmo; Rahayu, Endang Sri
Agritech Vol 22, No 2 (2002)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

Lactic acid bacteria (LAB) is known as fermentation agent in traditional food fermentation products in Indonesia, which also include fish products. Objectives of this research were to select LAB strain isolated from peda, terasi, salted fish and bekasam which were potential for dry starter preparation. This research were consisted of three parts : (a) selection of halophilic LAB strain (b) dry starter preparation by addition glycerol protectant, sucrose and starter neutralization and further testing its viability upon storage and (c) ability of the selected isolate to inhibit pathogenic and spoilage bacteria. Results of this reseacrh indicated there were 8 moderate halophilic LAB, they were Lb. plantarum (isolate EDI-14, DES-21, DES-24, and DES-26), Leuc. paramesenteroides (isolate DAN-7 and DAN-7), and Lv. casei subsp. casei (isolateDES-27). Addition of 5% glycerol protects LAB viability during storage. Addition of 17.7% sucrose in the media and neutralized the pH increase LAB viability during storage. Within 5 weeks of storage. The viability of Leuc. paramesenteroides (DAN-7) and Lb. plantarum (DES-26) decrease 10.4% (from 33 x 107 sel/g to 3.3 x 107 sel/g dried starter) and 9.77% (from 36 x 107 sel/g to 3.5 x 107 sel/g dried starter), respectively. Freeze drying process and dry storage did not affect the LAB isolate towards inhibiting the pathogenic bacteria and spoilage bacteria such as Salmonella choleraesius JCM 3919, Shigella, Escherichia coli FNCC 0091, Vibrio parahaemolyticus JCM 2147 (gram negative), Staphylococcus aureus FNCC 0091, and Morganella morganii NCTC 2847 (gram positive).
Pengembangan Metode Ekstraksi Alginat dari Rumput Laut Sargassum sp. sebagai Bahan Pengental Husni, Amir; Subaryono, Subaryono; Pranoto, Yudi; Taswir, Taswir; Ustadi, Ustadi
Agritech Vol 32, No 1 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

Indonesia has a lot of seaweed that have high potential as a producer of alginate, but the method of extraction has not been as expected. The objective of this study to develop a method of extraction of sodium alginate from seaweed Sargassum through the calcium alginate pathway. This study used different variations of the concentration of calcium chloride. The concentration of calcium chloride used varied 0.5, 0.75 and 1 M. As a control, the extraction of alginate performed through alginic acid pathway which was developed at the Center for Research Product Processing and Biotechnology of Marine and Fisheries, Jakarta. Quality parameters were observed including alginate yield, product appearance, viscosity and gel strength. The result showed that the yield of alginate produced successively for 32.67; 44.67 and 53.33 % and 31.67 % for controls. In appearance, the concentration of calcium chloride did not significantly affect the alginate product appearance, but darker when compared with the product extracted through the alginic acid. Viscosity alginate produced successively 149, 131 and 144 cP, while 304 cP for control. In general, the alginate gel strength extracted through of calcium alginate pathway is lower than the alginic acid pathway.ABSTRAKIndonesia  mempunyai  banyak  rumput  laut  yang  berpotensi  tinggi  sebagai penghasil  alginate,  namun  metode ekstraksinya belum sesuai yang diharapkan. Penelitian ini bertujuan untuk mengembangkan metode ekstraksi natrium alginat dari rumput laut Sargassum melalui jalur kalsium alginat. Dalam penelitian ini digunakan berbagai variasi konsentrasi kalsium klorida yang digunakan pada pemisahan alginat dari filtrat hasil ekstraksinya. Konsentrasi kalsium klorida yang digunakan divariasi 0,5; 0,75 dan 1 M. Sebagai kontrol dilakukan ekstraksi alginat melalui jalur asam alginat yang dikembangkan di Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Jakarta. Parameter kualitas alginat yang diamati meliputi rendemen alginat, kenampakan produk, viskositas dan kekuatan gel yang dihasilkan. Hasil pengamatan menunjukkan bahwa rendemen alginat yang dihasilkan berturut-turut sebesar 32,67; 44,67 dan 53,33 % dan untuk kontrol 31,67 %. Secara kenampakan, konsentrasi kalsium klorida tidak terlalu mempengaruhi kenampakan produk alginat yang dihasilkan, tetapi lebih gelap jika dibandingkan dengan produk hasil ekstraksi melalui jalur asam alginat. Viskositas alginat yang dihasilkan berturut-turut 149, 131 dan 144 cP, sementara untuk kontrol 304 cP. Secara umum kekuatan gel alginat yang dihasilkan dari jalur kalsium alginat lebih rendah dibandingkan dengan yang diekstrak melalui jalur asam alginat.
Komponen Bioaktif pada Madu Karet (Hevea brasiliensis) Madu Kaliandra (Calliandra callothyrsus) dan Madu Randu (Ceiba pentandra) Ustadi, Ustadi; Radiati, Lilik Eka; Thohari, Imam
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol 12, No 2 (2017)
Publisher : Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK)

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Abstract

The objectives of this study were to determine of bioactive compoounds from the different plants such as kapok, calliandra and rubber. The samples were obtained from Kediri (Calliandra), Sragen (Rubber) and Pasuruan (Kapok). The results showed the different of bioactive compounds. It had the following phenolic content ranged from 309.12 ± 33.40 mg/GAE kg (Kapok honey) to 557.93 ± 13.41 mg/GAE kg (Calliandra honey), flavonoid content ranged from 47.25 ± 1.49 mg QE/100 g (Kapok honey) to 156.27 ± 5.69 mg/GAE kg (Calliandra honey), vitamin C content ranged from 25.47 ± 1.62 mg/100 g (Kapok honey) to 31.04 ± 1.45 mg/100 g (Calliandra honey). The IC50 of Calliandra honey (3.36 ± 0.89 mg/mL) showed that antioxindat activity is higher than Rubber and Kapok honey (15.08  ± 1.49 mg/mL and 16.83 ± 1.23 mg/mL, respectively).  
IMMUNOSTIMULATORY EFFECT OF FATTY ACID FROM STAR FISH (ACANTHASTER PLANCI) ON LYMPHOCYTE PROLIFERATION IN-VITRO Achmad, M. Janib; Isnansetyo, Alim; Kasanah, Noer; Ustadi, Ustadi; Kamiso, Kamiso
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 3 (2014): December 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

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Abstract

The aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of star fish Acanthaster planci. A. planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated in column chromatography using silica gel and hexane-ethyl acetate-methanol. The active compound had been purified by Thin Layer Chromatography (TLC) and identified by Gas Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity was measured based on % Stimulation Index (SI) from sample absorbency and control absorbency. The result showed that the 3 fractions of hexane fraction exhibited lymphocyte proliferation activity. Fraction 1 was able to increase lymphocyte proliferation at 48 hours and 72 hours by 55% (80 µl/ml) and 88% (160 µl/ml) increase, and fraction 2 had 77% (160 µl/ml) and 86% (640 µl/ml) increase. Meanwhile, fraction 3 had 75% (640 µl/ml) and 89% (640 µl/ml) increase. Metabolite analysis of active fraction using GC-MS yielded a number of chemical constituents that was dominated by fatty acid. The study concluded that star fish A. planci from Ternate Island has a potential source of immunostimulator.
Bioformation of N-Acetylglucosamine from Shrimp Shell Chitin by Serratia marcescens PT-6 Cultured in various pH and Temperature Sari, Bekti Wulan; Isnaini, Nurul Binti; Puspita, Indun Dewi; Husni, Amir; Ustadi, Ustadi
Jurnal Perikanan Universitas Gadjah Mada Vol 19, No 1 (2017)
Publisher : Universitas Gadjah Mada

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The study aimed to determine the effect of pH and temperature on N-Acetylglucosamine (NAG) formation from shrimp shell chitin by Serratia marcescens PT-6 fermented in chitin broth medium. Parameters examined on NAG formation include bacteria growth, chitinase activity (U/ml), and NAG concentration in medium (mg/ ml). Bacteria growth was measured by turbidity (OD600), while chitinase activity and NAG concentration in the medium were analyzed quantitatively by colorimetric assay. The variation of initial pH examined in chitin broth medium was 5; 6; 7; 8, while temperature variation was 30°C; 37°C; dan 40°C. The results show that maximum concentration of NAG formed by Serratia marcescens PT-6 was 33.86 µg/ml on day-3 of fermentation, at pH 7 and temperature of 30°C. Chitinase activity on the same day was 0.002 U/ml, while OD600 value of the culture was 0.42 indicating the bacteria were in the log phase. This research implies that Serratia marcescens PT-6 is potential to be optimized further for the bioconversion process of NAG from shrimp shell waste through an enzymatic method by modifying its culture condition.
Aktivitas Enzim Pendegradasi Kitin dari Isolat SDI23 Asal Petis serta Karakterisasi pH dan Suhu Aktivitas Enzim Hasil Purifikasi Parsial Orinda, Eny; Puspita, Indun D.; Putra, Muhammad P.; Ustadi, Ustadi; Lelana, Iwan Y. B.
Jurnal Perikanan Universitas Gadjah Mada Vol 17, No 2 (2015)
Publisher : Universitas Gadjah Mada

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui aktivitas enzim pendegradasi kitin yang dihasilkan selama pertumbuhan dari lima isolat bakteri kitinolitik (SDI23, MDR23, SDI15, SDI13 dan BLT12) asal petis udang serta mengetahui karakteristik aktivitas enzim hasil pemurnian parsial pada berbagai suhu (30-55°C) dan pH (4-9). Kelima isolat ditumbuhkan pada medium koloidal kitin cair dengan suhu inkubasi 37°C selama 96 jam. Pengamatan terhadap jumlah bakteri (log CFU/ml) dan aktivitas spesifik enzim (U/mg) dilakukan setiap 12 jam. Aktivitas enzim pendegradasi kitin diukur secara kuantitatif, dengan mengukur jumlah N-asetilglukosamin yang dihasilkan dari hidrolisis koloidal kitin oleh enzim. Purifikasi parsial dilakukan dengan presipitasi ammonium sulfat bertingkat (20-80%) yang dilanjutkan dengan dialisis. Isolat SDI23, MDR23, SDI15 dan BLT12 menunjukkan waktu pertumbuhan optimum dengan jumlah sel tertinggi pada jam ke-48, sedangkan isolat SDI13 pada jam ke-24. Aktivitas spesifik enzim tertinggi ditunjukkan oleh isolat SDI23 sebesar 0,66 U/mg pada inkubasi selama 48 jam. Berdasarkanhasil tersebut, isolat SDI23 dipilih untuk memproduksi enzim pada tahap purifikasi parsial. Supernatan bebas sel dari media kultur isolat SDI23 disiapkan dari inkubasi SDI23 dalam medium koloidal kitin cair pada suhu 37oC selama 48 jam. Presipitasi dengan amonium sulfat pada fraksi 80% dan dialisis menghasilkan ekstrak kitinase kasar dengan nilai aktivitas spesifik enzim sebesar 0,072 U/mg dan 0,063 U/mg. Enzim hasil pemurnian parsial menunjukkan aktivitas optimum pada pH 6 dan suhu 45°C dengan nilai aktivitas spesifik enzim sebesar 0,09 U/mg.
Aplikasi Campuran Alginat Dari Sargassum Crassifolium Dan Gum Sebagai Pengental Textile Printing Subaryono, Subaryono; Tazwir, Tazwir; Husni, Amir; Ustadi, Ustadi; Pranoto, Yudi
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 2 (2015): Desember 2015
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

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Abstract

Penelitian aplikasi campuran alginat dari Sargassum crassifolium dan gum untuk meningkatkan viskositas alginat sebagai pengental pada textile printing telah dilakukan. Viskositas campuran alginat dengan guar gum, gum arab, dan locust bean gum diamati pada penyimpanan selama 8 jam. Produk terbaik diujikan sebagai pengental pada textile printing. Campuran alginat dengan guar gum pada perbandingan 90:10 dan 80:20 meningkatkan viskositas dan stabilitas alginat selama penyimpanan. Campuran alginat dengan gum arab dan locust bean gum akan menurunkan viskositas alginat sehingga tidak sesuai untuk aplikasi textile printing. Aplikasi campuran alginat dengan guar gum 90:10 dan 80:20 sebagai pengental pada tekstil printing menghasilkan produk akhir yang setara dengan pengental komersial manutex. 
Feasibility Study on the Profitability of Vannamei Shrimp Aquaculture on Coastal Area of Keburuhan Village, Purworejo Regency Djumanto, Djumanto; Ustadi, Ustadi; Rustadi, Rustadi; Triyatmo, Bambang
Aquacultura Indonesiana Vol 17, No 1 (2016)
Publisher : Indonesian Aquaculture Society (MAI)

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Abstract

Brackish water vannamei shrimp aquaculture in Keburuhan village of Purworejo Regency is one of the shrimp aquaculture activities developed since 2010. Shrimp aquaculture enteprises were done by local communities which use sandy land coated by plastic sheet with an area of 1000-4500 m2. Some farmers suffered losses due to lack of technical knowledge in shrimp aquaculture, environmental carrying capacity, ecological and economic feasibility. The aim of this study was to determine the general condition of vannamei shrimp aquaculture and shrimp farming feasibility. The study was conducted by field surveys, interviews and questionnaires to 30 farmers. The survey was conducted in May-June 2016 by purposive sampling of farmers who were members of a joint venture group of Minamulyo. The results showed shrimp pond size aquaculture area was varied, with a range of 1000-1500 m2, 1500-2000 m2, 2000-3000 m2 and 3000-4500 m2. An average fixed cost required for construction and operation of shrimp pond aquaculture was approximately IDR 6,620,934 to 12,606,666 million/plot/cycle shrimp production, while the variable costs of each cycle production ranged from IDR 45,876,733 to 78,861,666. The revenue and net profit varied according to pond size. The net gain for the smallest pond (1000-1500 m2) was IDR 48,702,332/plot/cycle, and the largest pond (3000-4500 m2) was IDR 58,131,666/plot/cycle. The shrimp farmers could recover the original investment incurred within a period of 4-6 months (one half cycles). Vannamei shrimp aquaculture in Keburuhan village of Purworejo regency brings a variety positive and negative impacts to the development of the economic, social and environment. Positive impact included increase of income and welfare of farmers, while the negative impact was a decline in the quality of the pond environment.