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Respon Molting, Pertumbuhan, dan Mortalitas Kepiting Bakau (Scylla olivacea) yang disuplementasi Vitomolt melalui Injeksi dan Pakan Buatan Wijaya, Yushinta; Aslamyah, Siti; Usman, Zainal
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 4 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Salah satu teknologi produksi kepiting cangkang lunak adalah menggunakan vitomolt untuk menstimulasi molting.  Penelitian bertujuan menganalisis respon molting, pertumbuhan, dan mortalitas kepiting bakau setelah diberikan vitomolt melalui injeksi dan pakan buatan. Penelitian dilaksanakan pada bulan Mei - Juli 2011, di Crabs Research Station yang terletak di Kabupaten Maros. Ada tiga perlakuan suplementasi vitomolt, yakni; secara tunggal melalui injeksi, kombinasi injeksi-pakan buatan, dan tanpa suplementasi vitomolt (kontrol), Hasil penelitian menunjukkan bahwa suplementasi vitomolt melalui injeksi dengan dosis 15 µg/g kepiting memberikan respon persentase molting tertinggi, yakni (84,00±5,48%), namun kombinasi injeksi (15 µg/g kepiting) dan pakan buatan (32.375 mg/kg pakan) memberikan respon molting yang lebih cepat.  Pada minggu kedua setelah perlakuan, kepiting yang molting pada perlakuan kombinasi adalah 14%, dibandingkan perlakuan lainnya, masing-masing 8% untuk perlakuan secara tunggal melalui injeksi dan 2% untuk kontrol.  Suplementasi vitomolt tidak memberikan pengaruh yang signifikan terhadap pertumbuhan setelah molting, namun memberikan pengaruh yang siginifikan pada mortalitas.  Mortalitas kepiting yang mendapat suplementasi vitomolt lebih rendah (6-8%) dibandingkan tanpa suplementasi vitomolt (24%). Kata kunci: vitomolt, injeksi, pakan buatan, kepiting cangkang lunak   One of the soft shell crab production technology is by application vitomolt to stimulate molting. The study aims to analyze the response of molting, growth, and mortality of mangrove crabs after being given vitomolt through injection and artificial feed. The experiment was conducted between May-July 2011, at Crabs Research Station in Maros Regency. There were two treatments of vitomolt and a control in this experiment, namely single treatment by injection, combination treatment by injection-artificial feed, and without vitomolt supplementation (control),  Results showed that supplementation of vitomolt through injection at a dose of 15 mg / g crab had highest percentage of molting (84.00 ± 5.48%), but a combination among injection (15 µg / g of crab) and artificial feed (32 375 mg / kg of feed) give faster molting response.  On the second weeks after treatment, there were 14% of crab had been molting in combination treatment, compared other treatments, respectively 8% for a single treatment by injection and 2% for control.  Vitomolt supplementation did not have a significant influence on growth after molting, but it gives a significant effect on mortality. Mortality of crabs that got vitomolt supplementation was lower (6-8%) compared without vitomolt supplementation (24%). Key words: vitomolt, Injection, artificial feed, soft shell crab
Effectivity of mulberry leaf extract on stimulating ekdisteroid hemolimph content and molting of mud crab (Scylla olivacea) Fujaya, Yushinta; Trijuno, Dody Dharmawan; Haryati, Haryati; Hasnidar, Hasnidar; Rusdi, Muhammad; Usman, Zainal
TORANI: Journal of Fisheries and Marine Science Vol 2, No 1 (2018)
Publisher : Marine Science and Fisheries Faculty, Hasanuddin University

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Abstract

This study aims to study the performance of phytoecdisteroids from mulberry leaves in molting stimulating of mangrove crabs. The research was carried out at the Research and Development Center for Aquaculture at the Faculty of Marine Sciences and Fisheries at Hasanuddin University, located in Bojo Village, Mallusetasi District, Barru Regency, South Sulawesi Province. Mangrove crabs (Scylla olivacea) weighing 45-55 g and carapace widths 60-65 mm were used as test animals. There were five doses of mulberry leaf extract applied through feed, namely: A) 1.1 mg / g feed, B) 1.9 mg / g feed, C) 2.7 mg / g feed, D) 3.5 mg / g feed. Crabs were kept individually in plastic boxes that are floated above the surface of the pond. During rearing, crabs are fed 3% dry fish per day which has been enriched with mulberry leaf extract. The parameters observed were ecdysteroid compounds contained in mulberry leaf extract, ecdysteroid content in hemolymph before and after application of mulberry leaf extract, and molting percentage. Identification of the ekdisteroid compounds contained in mulberry leaf extract was carried out using Thin Layer Chromatography (TLC) and Measurement of the content of ecdysteroids in crab hemolymh was carried out using Ultra Fast Liquid Chromatography (UFLC).  The results showed that the 3.5 mg dose of mulberry leaf extract / g feed gave the highest increase in ecdysteroid hemolymph, which was approximately 1760 ekdisteroid per mL hemolimph, while the dose of 1.1 mg / g feed only provided an increase of 100 ng ekdisteroid per mL hemolymph. The higher the dose of mulberry leaf extract in the feed, the higher the increase in the concentration of hemolymph ecdysteroids, but the high concentration of ecdystoid in the hemolymph does not guarantee molting.  The optimal dose of mulberry leaf extract in the feed stimulating molting was 2.4 mg / g of feed.Keywords: mulberry, phytoecdysteroid, molting, crab, aquaculture