Budi Untari
Pharmaceutical Program, Faculty of Sciences, University of Sriwijaya, Palembang 30662, South Sumatra

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Kuersetin-3-O-Glikosida (Rutin) dari Daun Ubi Karet (Manihot Glaziovii.M.A) Yusuf. M, Setiawati; Untari, Budi
Jurnal Penelitian Sains No 18 (2005)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Telah diekstrak senyawa Kuersetin-3-o-glikosida dari daun ubi karet (manihot glaziovii.m.a) dengan cara sokletasi menggunakan pelarut etanol teknis. Senyawa yang diperoleh berupa kristal kuning yang titik lelehnya 184,5° C -  186,5° C. Analisa terhadap kristal tersebut dengan alat Spektofotometri Ultra Violet dilakukan secara kualitatif dengan menggunakan pereaksi geser NaOCc 2,5%, NaOAc/H3BO3, AlCl3 5%/HCl 50%. Hasil penelitian menunjukkan gugus hidroksil (OH) yang terletak pada atom karbon C5, C7, C3, C4 dan glikosida pada atom karbon C3. Analisa data Spektroskopi Infra Merah menunjukkan adanya gugus-OH kuat pada 3417,0 cm-1, gugus –C=C- kuat pada 1651,5 cm-1, gugus –C=O kuat pada 1651,5 cm-1, gugus-C-H sedang pada 1293,3 cm-1 dan gugus –C-O sedang pada 1203,6 cm-1. Berdasarkan hasil analisa, kristal yang diperoleh adalah senyawa kuersetin-3-o-glikosida atau rutin (0,105%) berat kering.   
Pengembangan Metode Identifikasi Dna (Pij702) Menggunakan Prinsip Lisis Alkali dan Pengendapan dengan Natrium Asetat pH 2 Untari, Budi; Mardiyanto, Mardiyanto
Jurnal Penelitian Sains No 14 (2003)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Telah dilakukan perbaikan visualisasi plasmid bermarka melanin (pIJ 702) pada Streptomyces lividans Rekombinan dengan modifikasi metode lisis alkali. Larutan netrium asetat yang digunakan untuk mengendapkan DNA dalam metode lisis alkali didapat pada pH 2 memberikan hasil yang terbaik dalam menvisualisasikan pita plasmid pIJ 702. Dengan perendaman etidium bromida (50 mg dalam 50 mL air) lebih dari waktu normal juga tidak memperlihatkan melanin pengganggu, sedangkan larutan buffer penyimpan yang di dapat pada pH di bawah 7 tidak memberikan pengaruh yang berarti (p = 0,05). Hasil visualisasi fragmen Bam H1 plasmid pIJ 702 juga jelas pertanda metoda bebas dari pengaruh melanin. 
Telaah Penggunaan Senyawa Aktif Hasil Pirolisis Tempurung Kelapa Untuk Pembasmi Bakteri Perusak Kayu (Bacillus Cereus Var Mycoides). Bakti, I.A. Rivai; Untari, Budi; Anugrah, Dwi
Jurnal Penelitian Sains No 11 (2002)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Telah dilakukan uji daya hambat asap cair pirolisis tempurung kelapa terhadap pertumbuhan bakteri Bacillus cereus var mycoides. Bakteri Bacillus cereus var mycoides merupakan hasil isolasi bakteri kayu merawan (Hopea sp) yang ditanam dengan metode cawan tuang dan diuji pertumbuhannya dengan cara in vitro dengan menggunakan metode difusi kertas cakram. Hasil menunjukkan bahwa konsentrasi asap cair, yaitu 25%, 50%, 75% dan 100% v/v maka zona hambatannya yaitu sebesar 1,825; 2,425; 2,725 dan 3,10 cm. Senyawa antibakteri pada asap cair adalah senyawa fenol, 2-metoksi fenol, 2-metoksi-4-metil fenol, 4-etil-2-metoksi fenol dan 2,6-dimetoksi fenol.
Uji Anti Bakteri dan Identifikasi Senyawa Antibiotika yang Dihasilkan Streptomyces Sp bakti, I.A. Rivai; Untari, Budi; Oesman, Frida; Fitriani, Emilia
Jurnal Penelitian Sains No 6 (1999)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

It has been isolated identified anti bacterium of antibiotic compunds produced by streptomyces sp. The isolation of sterptomyces sp was done from three kinds of soil on Soak area Km . 6, Palembang Municipallity using Plating Technic Spread Method produced 4 streptomyces isolate,. The test of the blocked capacity againts the whole isolates (4 isolates) with the bactery terstor showed that TL.J2 isolate from corn plant land has biggest activities. The isolates of antibiotics produced using fermentation method for TL.J2 isolates by using solvent extraction method and continued with HPLC produced 4 pure compunds. The test of the blocked capacity againts the 4 pure compunds, just STP1 which still antibacteria activities. Furthermore, the identification of STP1 using IR Spectrophotometer and UV showed that the results can be clasified into Alifaitic antibiotic consisting of unsaturated ketod alifatic function with the primer amine and hydroxy as a substiutent.
CONCENTRATIONS TEST OF METHANE GAS FROM SWAMP FORAGE SILAGE WITH IN VITRO METHOD Sandi, Sofia; Gofar, Nuni; Sari, Meisji Liana; Yosi, Fitra; Untari, Budi; Kartika, Erra
BIOVALENTIA: Biological Research Journal Vol 3, No 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

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Abstract

Aims of this study to concentration test of methane gas from swamp forage silage with in vitro methods.This study used Completely Randomized Design with 3 treatments and each treatment consisted of 5 replications. The treatments used are as follows: P1 (100% kumpai tembaga grass (Hymenachne acutigluma)), P2 (50% kumpai tembaga grass (Hymenachne acutigluma) + 50% kemon air (Neptunia oleracea lour)), P3 (100% kemon air ( Neptunia oleracea lour)). The parameters observed N-Ammonia, total gas production, methane gas concentrations in vitro and VFA partially. The results of variance showed that treatment significantly different (P <0.05) with methane concentration  in vitro with highest gas concentration treatment of silage made from 50%  kumpai tembaga grass (Hymenachne acutigluma) and  50% kemon air (Neptunia oleracea lour).Keywords: Concentration, Methane, Silage Forage Swamp, In Vitro.
Oxidation and Acetylation of Ursolic and Oleanolic Acids Isolated from Fragraea fragrans fruits; Antiproliferation of P388 Leukemia Cells Basir, Dasril; Julinar, Julinar; Agustriana, Eva; Untari, Budi
Indonesian Journal of Chemistry Vol 14, No 3 (2014)
Publisher : Universitas Gadjah Mada

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Abstract

An interesting natural product chemistry aspect of Fragraea fragrans is that their fruits are richness with ursolic acid and its isomer oleanolic acid (3.05% of dried powder). As our continuous work on these inseparable structural isomeric triterpenes, this paper reports that 51.0% of inseparable 3-oxo-ursolic[3-oxo-oleanolic] acids and 48.6% of inseparable 3-acethyl-ursolic [3-acethyl-oleanolic] acids have already been made from those triterpenes as starting materials of the oxidized and acetylated compounds and evaluated their activity against P388 leukemia cells. The activity of 3-oxo-ursolic [3-oxo-oleanolic] acids with IC50 = 18.6 µg/mL exhibited three-fold more potent against P388 leukemia cell proliferations compared to ursolic [oleanolic] acids with IC50 = 53.5 µg/mL; while the 3-acethyl-ursolic [3-acethyl-oleanolic] acids with IC50 = 37.9 µg/mL showed two-fold more potent then their parent triterpenes (IC50 = 53.5 µg/mL) in the inhibition of P388 leukemia cell growth.