Umrah Umrah
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ANTAGONISITAS DAN EFEKTIVITAS Trichoderma sp DALAM MENEKAN PERKEMBANGAN Phytophthora palmivora PADA BUAH KAKAO Umrah, Umrah; Anggraini, Tjandra; Esyanti, Rizkita Rachmi; Aryantha, I Nyoman P.
AGROLAND Vol 16, No 1 (2009)
Publisher : AGROLAND

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Abstract

The purpose of the study was to select several Trichoderma sp based on its sporalation capabilities, and antagonistic character, and effectiveness to control P. palmivora on cocoa pod.  The study consisted of two stages. In the first stage, a Completely Randomised design was used which consisted of seven treatments with three replicates: T. viridae, collection of  Laboratory of Plant Pest and Diseases of Bogor Institute of Agriculture (T-A), T harzianum, collection of Laboratory of Plant Pest and Diseases of Bogor Institute of Agriculture (T-B), T harzianum,  collection of  Coffee and Cacao Research centre of Jember (T-C), T koningii, collection of  Coffee and Cacao Research Centre of Jember (T-D), T. Trichoderma sp 1, collection of Laboratory of Microbiology  Life Sciences Centre of Bandung Institute of Technology    (T-E), Trichoderma sp 2, collection of Laboratory of Microbiology  Life Sciences Centre of Bandung Institute of Technology (T-F) and Trichoderma sp, collection of Laboratory of Plant Pest and Diseases University of Tadulako (T-G). Parameters measured were sporulation capability of Trichoderma sp, percentage of antagonisticity of Trichoderma sp towards in vitro P. palmivora . The second stage of the study was to determine the effectiveness of Trichoderma antagonisticity, either individual or mixed cultures in controlling the development of  P. palmivora on cocoa pod. The study was designed with seven treatments with three replicates:  T-D, T-E, T-G, T-DE  (mixed culture of T-D and T-E),  T-DG  (mixed culture of T-D and T-G), T-EG ( mixed culture of T-E and T-G), T-DEG (mixed culture of  T-D, T-E, and T-G). The results of the study showed that at in vitro condition the kinds of Trichoderma sp which had the highest antagonisticity on P. palmivora were  Trichoderma sp (T-G, T-E and T-D). They were effective to control P. palmivora on cocoo pod either in individual or mixed cultures.  However, T-G will be further developed due to its highest effectiveness as a biological control compared to the others.
Perilaku dan Penentuan Oviposisi dari Parasitoid Telur Hadronotus leptocorisae (Hymenoptera) pada Telur Hama Bulir Padi Leptocorisa acuta (Hemiptera) Anggraeni, Tjandra; Jamili, Aisah; Umrah, Umrah
Biocelebes Vol 4, No 2 (2010)
Publisher : Biocelebes

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The research of behavior and host determination for the oviposition of egg parasitoid of Hadronotus leptocorisae on egg of the rice seed pests (Leptocorisa acuta) was carried out. H. leptocorisae collected from rice field were inserted into the tube containing of eggs of L. acuta which were divided into three group based on their ages; young eggs (0-1 days); mature eggs (2-3 days) and old eggs (4-5 days). The results showed that female H. leptocorisae prefer to oviposition on young eggs. Descriptive observation indicated that before eggs were laid, female H. leptocorisae inspect L. acuta eggs by rubbing of L. acuta eggs with its antenna and ovipositor. Furthermore, oviposition of H. leptocorisae eggs was begun by holing of L. acuta eggs and was followed by putting its eggs by its ovipositor. That behavior is its strategies to survive. The results of this study can contribute to answer pest L. acuta problems which are often found in rice fields. By the appropriate monitoring of L. acuta eggs in rice field, the disposal time of H. leptocorisae could be done effectively and efficiently.Key words: Hadronous leptocorisae; Leptocorisa acuta; egg parasitoid.
Pemanfaatan Limbah Cair Tahu (Whey Tahu) Sebagai Media Tumbuh Acetobacter xylinum untuk Memproduksi Nata Alwi, Muhammad; Rahmiati, Rahmiati; Umrah, Umrah
Biocelebes Vol 5, No 2 (2011)
Publisher : Biocelebes

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Research on “Waste Utilization Tahu” (Whey Tahu) as growth media for producing nata Acetobacter xylinum has been carried out from February to March 2011 with the aim of utilizing wastewater tahu (whey tahu) as a growing medium nata-producing Acetobacter xylinum as a source food and sucrose concentration and pH optimal for the growth of bacteria A. xylinum in producing nata. Method used in this research is experiment by 9 combinations of treatments and 3 repeation. Design was using a “Completely Randomized with composed with factorial” the factors is sugar concentration and secondary factor is acidity level (pH) . The Result of research indicate that nata and thick and also different at all of given treatments. The interaction between sucrose and pH provide a response to differences in thickness of nata formed. The best treatment which is interaction of sugar 10% and pH 5 in thick forming nata that is and 0,43 cm. and rendemen nata 15.92%. Key words: Whey tahu, Acetobacter xylinum, and Nata.
Pemanfaatan Ampas Tahu Sebagai Bahan Baku Kecap Melalui Bioproses Rhizopus spp. Umrah, Umrah; Asrinawaty, Nur; Alwi, Muhammad
Biocelebes Vol 5, No 2 (2011)
Publisher : Biocelebes

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Penelitian Pemanfaatan Ampas Tahu Sebagai Bahan Baku Kecap Melalui Bioproses R.hizopus spp. telah dilaksanakan pada bulan April-Juni 2011. Adapun tujuan dan penelitian ini yaitu untuk mengetahui pengaruh lama bioproses Rhizopus spp., terhadap kadar protein kecap ampas tahu dan kesesuaian dengan standar mutu kecap di Indonesia serta pengaruhnya terhadap aroma dan cita rasa kecap ampas tahu. Penelitian dilakukan di laboratorium Bioteknologi dan di Laboratorium Agroteknologi. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap dengan perlakuan lama bioproses Rhizopus spp.: P0 (tanpa fermentasi), P1 (fermentasi satu han). P2 (fermentasi dau han) dan P3 (fermnetasi tiga han). Penentuan kadan protein dilakukan dengan menggunakan metode spektrofotometer. Hasil penelitian menunjukkan bahwa lama bioproses Rhizopus spp. berpengaruh nyata pada kadar protein kecap ampas tahu dengan perlakuan yang terbaik adalah P2 (fermentasi 2 han) sebesar 7,29%. Selama 3 hari proses fermentasi, kadar protein meningkat. Kadar protein tersebut telah sesuai dengan standar mutu kecap nomor satu di Indonesia. Lama bioproses Rhizopus spp. berpengaruh nyata pada aroma kecap ampas tahu tetapi tidak berpengaruh pada cita rasa kecap ampas tahu. Key words: Ampas tahu, kecap , Rhizopus spp., dan kadar protein.
Uji Keefektivan Formula Trichoderma sp. Sediaan Tablet Sebagai Dekompuser Limbah Organik Ampas Sagu Menjadi Biokompos Umrah, Umrah; Alwi, Muhammad; Maddo, Muh. Arsal N.
Biocelebes Vol 5, No 2 (2011)
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Research entitle of “Bioactive compost from sago waste and chicken manure through bioprocess using Trichoderma sp.” have been conducted during March and July 2011. This research have been done in Biotechnology Laboratory, Science Fakulty of Tadulako University. This aims of this study are to produce bioactive compost from waste of sago pulp and chicken manure through bioprocess Trichoderma sp., also to determine the optimum dose of inoculums of Trichoderma sp.. This study was done experimentally and it was based on a completely randomized design. Result of this study showed that P2 (substrate + 2 tablets inoculum/kg substrate) was the best treatment. Key words: Bioactive compost , sago waste, chicken manure, Trichoderma sp.
Formulasi Media Tumbuh Acetobacter xylinum Dari Bahan Limbah Cair Tempe dan Air Kelapa Untuk Produksi Nata De Soyacoco Alwi, Muhammad; Lindhemuthianingrum, Andi; Umrah, Umrah
Biocelebes Vol 5, No 2 (2011)
Publisher : Biocelebes

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The impact of tempeh liquid-waste (Limbah Cair Tempe, LCT) as Contaminant has become a serious environmental problem. This study was design as an alternative problem solving related to that issue. Combination of tempeh liquid-waste and coconut water (Air Kelapa, AK) can be utilized as a medium of nata de soyacoco production. This research was aimed to obtain the best medium formulation for Acetobacter xylinum in order to produce the bacterial cellulose. This experiment was arranged in completely randomized design with 6 treatments and 3 replications. The treatments were P0 (LCT 0% + AK 0%), P1 (LCT 0% + AK 100%), P2 (LCT 25% + AK 75%), P3 (LCT 50% + AK 50%), P4 (LCT 75% + AK 25%), and P5 (LCT 100% + AK 0%). Parameters observed in this experiment were the days appear of nata, thickness, fresh weight, rendement and texture of nata which tested organolepticly. The best medium formulation for nata de soyacoco production was P4 (LCT 75% + AK 25%), which resulted 1.04 cm thickness, 139.48 gram fresh weight, 42.27% rendement and 1.7 of texture value. Key words: tempeh liquid water, coconut water, Acetobacter xylinum.
Identifikasi Actinomycetes yang Terdapat Pada Tanah di sekitar Danau Lindu Sulawesi Tengah Alwi, Muhammad; Merdekawaty, Latifah; Umrah, Umrah
Biocelebes Vol 6, No 1 (2012)
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The research about “Identification of Actynomycetes from soil around of Lindu Lake” from September to Desember 2010 the purpose was receiving, to identificate, and screening of Actinomycetes from soil around of Lindu Lake. The metodology is descriptive it means that all character data of isolat fenotive Actynomicetes were reSp.sentatively to be applied for identificating conventionally by applying the key book of identification. The result of this research was obtained six isolats Actynomycetes resepsentatively. They have been chosen to represnt the whole of isolat Actynomycetes nine isolat which were successful isolated from soil around of Lindu Lake. The result of identification obtained three famalies of Actynomycetes consisted of four genus and six Sp.ecies. They were Streptomyces Sp.1, Steptomyces griseus, Streptomyces roseoticillatrus, Herbidospora cretaceae, Micromonospora, and Thermomonospora formonensis. There were many genus of Streptomyces from six isolats. Key words: Actynomycetes, identification, and Lindu Lake.
Uji Kemampuan Formula Beauveria bassiana Balsamo. Bentuk Sediaan Tablet Untuk Mengendalikan Penggerek Buah Kakao Conopomorpha cramerella Snellen. Rahayu, Rahayu; Umrah, Umrah
Biocelebes Vol 6, No 1 (2012)
Publisher : Biocelebes

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The studied of Ability of Formula Beauveria bassiana Balsamo. in tablets forms to Control the Cocoa Pod Borer/CPB (Conopomorpha cramerella Snellen.) have been carried out from April to Juny 2011. The aim of this studies was to determine the ability and where the of formula Beauveria bassiana Balsamo. efficient and effectivines to control it. The studied was designed in Completely Randomized Design (CRD) whit 9 treatments as follows: P01(control without treatment), P02 (control with aquades), P1(5 tablets/liter), P2(10 tablets/liter), P3(15 tablet streatment), P4 (20 tablets/liter) ,P5 (25 tablets/liter), P6(30 tablets/liter), and P7(35 tablets/liter) and for it treatment were made by 3 replications. The Parameter in this study were percentage of dead pupae test, the number of test pupae imago, pupa with in 50% of test dies and test and description of the pupa cocon are infected. The results showed that the formula B. bassiana Balsamo. able to control the CPB, this indicated by percentage pupa mortality increased with increasing concentration of the treatment. The percentage of pupae that become imago decreased with increasing concentrations of P01(93.33%), P02 (80%), P1(56.66%), P2(40%), P3(36, 66%), P4(36, 66%), P5(30%), P6 (13.33%), P7(13.33%). When 50% of test pupae diefaster with increasing concentration of the treatment. While the description of the pupa infected with the texture of stiff, hard, body is white and ratherunpleasantodor. The analysis of the percentage, average of dead pupae showed at the P4 test (53.3%) was the most effective hiss treatment. Key words: Ability, formula, Beauveria bassiana Balsamo, C.cramerella.
Keefektivan Metarhizium anisopliae Sebagai Agen Pengendali Hayati Terhadap Larva Lalat Musca domestica L. Amiruddin, Muhammad; Umrah, Umrah; Alwi, Muhammad
Biocelebes Vol 6, No 1 (2012)
Publisher : Biocelebes

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Research on the "effectiveness of the fungus Metarhizium anisopliae as a biological control agent against the larvae of the fly Musca domestica (L)” was done and focusingin determination of the effectiveness of this fungus as a biological control agent against Musca domestica larvae and to determine LC (Lethal Concentration) 50 and LC 90 on this biological control proceses. This study was designed in a completely randomized design consisting of 6 treatments and 3 replications. Arrangement of treatments i.e. the concentration of M. anisopliae in distilled water was; Po = distilled water without spore suspension, P1 = 5.1 × 1010 spores / mL, P2 = 2.0 × 1010 spores / mL, P3 = 1.0 × 1010 spores / mL, P4 = 5.1 × 109 spores / mL, and P5 = 2.0 × 109 spores / mL. Based on the observations, the mortality percentage rate was reached 93.33% as it in the treatment of spora concentration of 5.1 x1010 spores / mL.Probit analysis showed that LC50 was 8.3 x109 spores / mL with the range between the lower limit and the upper limit were 6.24 x109 spores / mL -11.43 x109 spores / mL. While the LC 90 was 5.0 x1010 spores / mL with the range of the lower limit until the upper limit were 2.8 x1010 spores / mL - 9.9 x1011 spores / mL. Key words: Metarhizium anisopliae, Musca domestica, effectiveness, mortality, biological control
Skrining Aspergillus Antagonis Terhadap Phythophthora palmivora Butler. Penyebab Penyakit Busuk Buah Kakao di Sulawesi Tengah Husain, Fitriani; Umrah, Umrah; Alwi, Muhammad
Biocelebes Vol 6, No 2 (2012)
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The study about “Screening of Aspergillus antagonistic to Phytopthora palmivora causing black pod disease of cocoa at Sulawesi Tengah” has been carried out during three months. The purpose of this study is how to getting the best of local isolats of Aspergillus sp. antagonistic that can be controlling of Phytopthora palmivora. The method of this study is exploring the local Aspergillus fungi as biological control of the P. palmivora causing black pod disease of cocoa in five Districts central of production kakao, such as Donggala District, Parigi Moutong, Poso, Morowali, and Buol. Parameter observed were antagonist test in vitro and pra in vivo on the cocoa. The result of this study was obtained fourteen isolats of Aspergillus sp namely are (1) Poso District: L-1, L-2, L-3 ; (2) Morowali District, L-4, L-5, L-6; (3) Donggala District, L-7, L-8, L-9; (4) Parimo District , L-10, L-11, L-12; (5) Buol District, L-13, L-14. All isolates of Aspergillus sp. were antagonistic in vitro on P. palmivora, but only six isolates, namely are L-1, L-10, L14, L-4, L-6, dan L-8 were have the best ability to controlling P. Palmivora on the cocoa pod. Key words: Aspergillus sp., antagonism, Phytophthora palmivora and Cocoa pod.