Niken Ulupi
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Pengaruh Penambahan Tepung Tapioka dan Es Batu pada Berbagai Tingkat yang Berbeda terhadap Kualitas Fisik Bakso Sapi (Komariah), Komariah; Ulupi, Niken; Fatriana, Yani
Buletin Peternakan Vol 28, No 2 (2004): Buletin Peternakan Vol. 28 (2) Mei 2004
Publisher : Fakultas Peternakan UGM

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PENGARUH PENABURAN ZEOLIT PADA LANTAI LITTER TERHADAPPERSENTASE KARKAS DAN KOMPQN(EN NON KARKAS AYAM PEDAGING PADA KEPADATAN KANDANG YANG BERBEDA Kardaya, Dede; Ulupi, Niken
JURNAL PETERNAKAN Vol 2, No 2 (2005): September 2005
Publisher : JURNAL PETERNAKAN

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Spreading zeolites on litter floor of broiler house at rate of 5.0 kg/m2 increased broiler carcass percentages up to 5.41 percents. Effect of zeolites-spread litters on broiler carcass and non-carcaSs components under different floor spaces had been performed in five weeks. As many as 324 chicks of a day-old Hubbard strain were fed with rations contained 3,000 -3,100 Kcal/I<g ME and 21 %crude protein during starter period and fed with the ration contained 20% crude protein duringfinisher period. The chicks were allocated to three different floor spaces (10, 12, and 14 heads/m2) and three different zeolites-spread litters (0.0, 2.5, and 5.0 kg zeolites/m2) under completely randomized design with factor 3 x 3 and 3 replications. Parameters concerned of present research including percentages of both carcasses and non-carcass components were analyzed with analysis of variance (ANOV A) and least .significant different.Results of the preseqt research showed that there was no interaction effect between floor spaces and zeolites-spread litters on all parameters concerned. Floor spaces affected the percentages of gizzard, kidney, and spleen significantly (p<O.05) but neither carcass nor abdominal fat percentage was af.fectedby the floor space. The zeolites-spread litters only affected significantly (P<O.05) on carcass percentage. Spreading zeolites at rates of5.0 kg/m2 on litter floor resulted in the highest carcass percentage (p<O.05) while rates of 25 kg/m2 resulted the same carcass pen:entage as the rates of 0.0 kg/m2. It was concluded that spreading zeolites at rates of 5.0 kg/m2 on litter floor under different floor spaces increased can:ass percentages up to 5.41 percents.
The Role of Triglyceride Lipase, Fatty Acid Synthase and Fatty Acid Binding Protein Family Genes on Fat Metabolism of Broiler Chickens Ulupi, Niken; Sumantri, C
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 25, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development

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Broiler chicken has high fat content, especially abdominal and subcutan fat which reduced carcass quality and efficiency of feed energy. Genetic approach could be potentially applied to reduce high abdominal and intramuscular fat in broiler chicken through the selection program at gene level related to fat metabolism. This paper describes the metabolism and biosynthesis of body fat and the role of its controlled genes. Fat synthesis is controlled by feed quality and metabolism and biosynthesis process occurred in liver. These processes are controlled by many family genes, but certain genes have dominant role in the process; those are triglyceride lipase genes, fatty acid synthase genes and fatty acid binding protein genes. Expression of fatty acid synthase genes has positive correlation with fat content in liver and intramuscular. Expression of fatty acid binding protein genes was related to the increased abdominal fat deposit. These genes are polymorphic, so that they can be used as a genetic marker in selection to optimize feed efficiency, to minimize abdominal fat and to increase economic value of broiler chicken. Key words: Fat, triglyceride lipase genes, fatty acid synthase genes, fatty acid binding protein genes
IDENTIFIKASI KERAGAMAN GEN TOLL-LIKE RECEPTOR-4 PADA AYAM TOLAKI, AYAM PETELUR KOMERSIAL DAN AYAM BROILER ulupi, Niken
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 1, No 1 (2014)
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

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Gen Toll-like Receptor-4 (TLR4) adalah salah satu gen yang mengontrol ketahanan ayam terhadap Salmonella sp. melalui respons imun non spesifik. Gen tersebut dapat dijadikan sebagai marka genetik pada ayam apabila diketahui keragamannya. Oleh sebab itu tujuan penelitian ini adalah untuk mengidentifikasi keragaman gen TLR4 pada ayam Tolaki yang dibandingkan dengan ayam ras yang ada di Indonesia. Total sampel ada 126 terdiri dari ayam Tolaki (82 ekor), ayam petelur (20 ekor) dan ayam broiler (24 ekor). Tahapan identifikasi meliputi ekstraksi DNA genom, amplifikasi PCR gen TLR4 (220 pb, di exon 2), dan metode RFLP, menggunakan enzim restriksi MscI. Data yang dianalisis meliputi frekuensi alel dan frekuensi genotipe, nilai heterozigositas, dan Polymorphic Informative Content (PIC). Hasil yang diperoleh : gen TLR4|MscI pada ayam Tolaki, petelur dan broiler bersifat polimorfik, dengan ditemukan dua alel yaitu alel A dan G. Frekuensi alel G (pembawa sifat resisten) pada ayam Tolaki, broiler dan ayam petelur adalah 0.77, 0.87 dan 0.20. Dari uji x2 diketahui bahwa genotipe gen TLR4|MscI ayam Tolaki berada dalam keseimbangan Hardy-Weinberg, dengan nilai Ho dan He sebesar 0.31 dan 0.29. Nilai PIC gen TLR4 ayam Tolaki (0.25) termasuk kategori sedang. Berdasarkan hasil tersebut gen TLR4|MscI dapat digunakan sebagai penciri genetik untuk sifat ketahanan ayam terhadap infeksi Salmonella sp. pada ayam Tolaki.Kata kunci : keragaman genetik, gen TLR4, Salmonella sp, respons imun non spesifik
EFFECTS OF ZEOLITES ON LITTER FLOOR ON PERFORMENT OF BROILER Kardaya, Dede; Ulupi, Niken
Jurnal Zeolit Indonesia Vol 5, No 2 (2006)
Publisher : Jurnal Zeolit Indonesia

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Effect of zeolites-spread litters on broiler performances under different floor spaces had been performed in five weeks. As many as 324 chicks of a day-old Hubbard strain were fed rations contained 3,000 – 3,100 Kcal/Kg ME and 21% crude protein during starter period and fed the ratio contained 20% crude protein with similar energy during finisher period. The chicks were randomly allocated to three different floor spaces (10, 12, and 14 heads/m2) and three different zeolites spread litters (0.0, 2.5, and 5.0 kg zeolites/m2) under completely randomized design with factor 3 x 3 and 3 replications. Parameters concerned of present research including feed intake, live weight gain, and mortality rate were analyzed with analysis of variance and least significant different. Results of the present research showed that there was no interaction effect between floor spaces and zeolites spread litters on all parameters concerned. Floor spaces affected both feed intake and feed conversion significantly (P
EFFECT OF ZEOLITES SPEREADING ON LITTERS TO BROILER PERFORMANCES REARED UNDER DIFFERENT FLOOR SPACES Kardaya, Dede; Ulupi, Niken
Jurnal Zeolit Indonesia Vol 5, No 1 (2006)
Publisher : Jurnal Zeolit Indonesia

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Effect of zeolites spreading on litters to broiler performances under different floor spaces had been performed in five weeks. As many as 324 chicks of a day-old Hubbard strain were fed rations contained 3,000 – 3,100 Kcal/Kg ME and 21% crude protein during starter period and fed the ration contained 20% crude protein with similar energy during finisher period. The chicks were randomly allocated to three different floor spaces (10, 12, and 14 heads/m2) and three different zeolites spread-litters (0.0, 2.5, and 5.0 kg zeolites/m2) under completely randomized design with factor 3 x 3 and 3 replications. Parameters concerned of present research including feed intake, live weight gain, and mortality rate were analyzed with analysis of variance (ANOVA) and least significant different (LSD). Results of the present research showed that there was no interaction effect between floor spaces and zeolites spread litters on all parameters concerned. Floor spaces affected both feed intake (P
DETEKSI GEN Mx AYAM TOLAKI MENGGUNAKAN TEKNIK EKSTRAKSI DNA YANG BERBEDA Pagala, Muhammad Amrullah; Ulupi, Niken
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 1, No 1 (2014): JITRO, September
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

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Penelitian ini bertujuan untuk mencari metode yang efektif dalam teknik ekstraksi DNA dari gen Mx (Myxovirus) ayam Tolaki. Gen Mx adalah gen yang bertanggungjawab terhadap kemampuan ayam untuk mertespon serangan penyakit viral seperti penyakit Flu Burung dan New Castle Disesase. Penelitian ini dilaksanakan mulai bulan April sampai dengan bulan Agustus 2013.  Sampel penelitan terdiri atas 25 ekor ayam Tolaki dewasa, yang diperoleh dari pemeliharaan secara ekstensif oleh masyarakat di Kabupaten Konawe. Penelitian ini menggunakan kedua metode ekstraksi DNA yakni metode ekstraksi DNA secara konvensional dan metode Kit ekstraksi DNA.  Selanjutnya hasil ekstraksi DNA diamplifikasi menggunakan mesin PCR. Produk DNA kedua metode tersebut kemudian dibandngkan dan dianalisis secara kualitatif. Secara umum kedua metode tersebut cukup efektif untuk menghasilkan kualitas DNA gen Mx yang baik. Ekstraksi DNA gen Mx dari kedua metode tersebut menghasilkan ukuran DNA dengan panjang 299 pb.   Kata Kunci : Gen Mx, Ayam Tolaki, Ekstraksi DNA, PCR
IDENTIFIKASI KERAGAMAN GEN TOLL-LIKE RECEPTOR-4 PADA AYAM TOLAKI, AYAM PETELUR KOMERSIAL DAN AYAM BROILER Ulupi, Niken; Pagala, Muhammad Amrullah
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 1, No 1 (2014): JITRO, September
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

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ABSTRAK Gen Toll-like Receptor 4 (TLR4) adalah salah satu gen yang mengontrol ketahanan ayam terhadap serangan Salmonella sp, melalui respon immun non-specific. Gen ini dapat digunakan sebagai marka genetik pada ayam.  Oleh karena itu penelitian ini bertujuan mengevaluasi polimorfisme genetik gen TLR4 pada beberapa jenis ayam (Ayam Tolaki, Ayam Ras pedaging dan petelur). Total sampel sebanyak 126. Penelitian ini melalui 3 tahapan yakni ekstraksi DNA, amplifikasi PCR (dengan ukuran DNA 220 pb pada ekson 2), dan metode RFLP menggunakan  enzim MscI.  Hasil penelitian menunjukkan gen TLR4 | MscI bersifat polimorfik pada semua jenis ayam. Diperoleh 2 alel (A dan G). Frekuensi alel G (membawa sfat resisten) pada ayam Tolaki, Ras pedaging, dan petelur masing-masing 0.77, 0.87 dan 0.20. Nilaix2menunjukkan genTLR4|MscI berada dalan keseimbangan Hardy-Weinberg dengan Ho dan He 0,31 dan 0,29. Nilai PIC 0,25 termasuk kategori medium. Gen TLR4| MscI dapat digunakan sebagai marka genetik sifat ketahanan ayam Tolaki terhadap infeksi Salmonellasp.   Keywords : Polimorfik, Gen TLR4, Salmonella sp., Non-Specific Immune Response
g.640T>C Polymorphism of the TGF-β2 Gene is Associated with Salmonella pullorum Resistance in Indonesian Chickens Muhsinin, Muhammad; Ulupi, Niken; Gunawan, Asep; Wibawan, I Wayan Teguh; Sumantri, Cece
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The objectives of this study were to identify polymorphism of transforming growth factor β2 (TGF-β2) gene associated with Salmonella pullorum resistance in Indonesian chickens. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays were used to genotype Indonesian chickens. Analysis of polymorphism was conducted by using PopGen 3.2 software. The effect of genotype on S. pullorum resistance was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 278 chickens from 7 Indonesian chicken populations (Sentul, Merawang, Pelung, Kampung, Parent Cobb broiler, The F1 crossbreed of Kampung x Parent Cobb broiler (KB) and F2 KB x KB. The product of amplification was 284 bp. The TGF-β2| RsaI locus was polymorphic in all populations, producing two alleles (T and C) and three genotypes (TT, CT, and CC). The result from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the C allele in all populations. The χ2 analysis showed that the 6 chicken populations were deviated from Hardy-Weinberg equilibrium, with the exception of the Parent Cobb broiler chicken. The association result showed that TT genotype was significantly associated with S. pullorum resistance in Sentul chicken. Although the leukocyte concentration, leukocyte differentiation and H/L ratio in Sentul chicken with three of TGF-β2 genotypes (TT, TC, and CC) were not statistically different. In conclusion, polymorphism in the TGF-β2 chicken gene can be used as a candidate marker to increase S. pullorum immune response.
Pengaruh Penambahan Tepung Tapioka dan Es Batu pada Berbagai Tingkat yang Berbeda terhadap Kualitas Fisik Bakso Sapi (Komariah), Komariah; Ulupi, Niken; Fatriana, Yani
Buletin Peternakan Vol 28, No 2 (2004): Buletin Peternakan Vol. 28 (2) Mei 2004
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

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