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Uji Aktivitas Antibakteri dari Ekstrak Rumput Mutiara (Hedyotis corymbosa (L.) Lamk) Mukmilah, Lela; Udin, Zalinar; Lisnawati, Elis
VALENSI Vol 2, No 5 (2012): Jurnal Valensi Volume 2//No.5//November 2012
Publisher : Program Studi Kimia, Fakultas Sains dan Teknologi, UIN Syarif Hidayatullah Jakarta

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Abstract

Rumput mutiara merupakan tanaman yang berguna sebagai obat tradisional, tanaman ini termasuk kedalam famili tumbuhan Rubiaceae. digunakan sebagai obat alternatif penyebab penyakit infeksi yang disebabkan karena masuknya mikroorganisme kedalam tubuh. Salah satu mikroorganisme yang mengakibatkan penyakit ini adalah bakteri ( Amonim, 1993). Antibakteri adalah senyawa kimia yang dapat membunuh atau memghambat pertumbuhan mikroorganisme penyebab penyakit dengan kerusakan minimal jaringan induknya. Dilakukan penelitian aktivitas antibakteri dari ekstrak rumput mutiara (Hedyotis corimbosa (L.) Lamk) terhadap pertumbuhan beberapa bakteri, yang dlilakukan melalui 4 tahapan yaitu tahapan maserasi, tahapan ekstraksi fraksinasi, tahap pengujian fitokimia, dan diteruskan dengan tahap pengujian aktivitas antibakteri menggunakan metode difusi agar, parameter yang digunakan adalah dengan melihat zona terang yang dihasilkan (zona yang tidak terjadi pertumbuhan). Hasil penelitian menunjukkan bahwa dalam ekstrak rumput mutiara terdapat zat aktif yang berfungsi sebagai antibakteri yang terdapat dalam fraksi metilen klorida, pada fraksi ini aktivitas paling baik dihasilkan oleh bakteri S. Disentry dengan diameter daerah hambatan sebesar 27,5 mm.
Cytotoxicity Assay From Fractions of Hedyotis corymbosa Extract Against Breast Cancer Cell Line T47D Andriyani, Rina; Risdian, Chandra; Udin, Zalinar
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Drug discovery for cancer medication is the most important effort that researcher do  at  this time. Indonesia bio diversities  have  possibility  as  a cancer medicine  sources. Finding a herbal medicine for cancer treatment is a first step to find a right cancer medicine in the future.This research has already completed for the earlier another research.Some fractions of Hedyotis corymbosa extract has been analyzed using Sulforhodamine B method with  UV wavelength  515 nm against T47D cell line, a human breast cancer. There are Hexane extract, Methylene chloride extract and Ethyl acetate extract,and give inhibitory concentration 50 (IC50) of 33.45 1g/ml, 54.59 1g/ml and 52.58 1g/ml, respectively. Ethanolic extract,  itself  has  IC50 of 61.57 1g/ml, whereas IC50 value of  Cisplatinum is 9.63 1g/ml. There is a difference between the ethanolic extracts with the other fraction.
Molecular Docking of Robustaflavone Derivatives on Pi3k Protein Target and The Correlation With Its Cytotoxic Activity on Cancer Cells Handayani, Sri; Udin, Zalinar
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Robustaflavone  derivatives  have  cytotoxic  effect  on  cancer  cell.  Inhibition  of  PI3K  on cancer  cell  has  correlation  with  apoptotic  induction.  The  aim  of  this  research  is  to  observe interaction  from  robustaflavone  derivatives  on  PI3K  receptor  that  may  give  contribution  to  the cytotoxic effect of the compounds on cancer cell line. Geometric optimization of robustaflavone derivatives  structured  was  done  used  Hyperchem  7.5  software.  Arguslab  4.01  software  with GAdock  method  applied  for  the  docking  step.  The  docking  result  showed  that  robustaflavone 7,4,7-  trimethyl  ether  (RTE)  has  affinity  on  PI3K  target  receptor  which  gave  the  lowest  Gibbs free energy (∆G -10,855 kcal/mol).Key words: robustaflavone, PI3K, cancer, docking
SENYAWA AKTIF ANTIKANKER PAYUDARA DAN ANTIMALARIA DARI TUMBUHAN DADAP AYAM (ERHYTHRINA VALERIEGATA) SECARA IN VITRO Herlina, Tati; Syafruddin, Syafruddin; Udin, Zalinar
Jurnal Manusia dan Lingkungan (Journal of People and Environment) Vol 19, No 1 (2012)
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

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Abstract

Tumbuhan Erythrina variegata (Leguminosae) secara tradisional dikenal oleh masyarakat Indonesia sebagai obat antikanker dan antimalaria. Bagian tumbuhan ini yang biasa digunakan sebagai bahan pengobatan adalah daun dan kulit batang, tapi kandungan senyawa kimia aktif biologisnya belum banyak dilaporkan. Tujuan penelitian ini adalah untuk mengungkapkan senyawa aktif antikanker dan antimalaria secara in vitro yang terdapat di dalam daun dan kulit batang E. variegata. Penelitian dilakukan dengan cara ekstraksi metanol dan fraksionasi dari daun dan kulit batang E. variegata yang dipandu dengan uji antikanker secara in vitro terhadap sel kanker payudara T47D menggunakan metode Sulforodamin B (SRB) dan uji antimalaria secara in vitro terhadap Plasmodium falciparum 3D7 (sensitif klorokuin) dan K1 (resisten klorokuin) menggunakan metode laktatdehidrogenase (LDH). Selanjutnya dilakukan pemisahan fraksi etil asetat daun dan kulit batang E. variegata yang dipantau dengan uji antikanker dan antimalaria secara in vitro menggunakan kombinasi kolom kromatografi diperoleh tiga senyawa aktif (1, 2, dan 3). Struktur kimia senyawa aktif (1, 2, dan 3) ditetapkan berdasarkan data-data spektroskopi dan diidentifikasikan sebagai turunan triterpenoid pentasiklik glikosida(1); flavonoid, eristagallin A (2); dan steroid, (22E)-5α,8α-epidioksiergosta-6,22-dien-3β-ol (3). Senyawa (1) menunjukkan aktivitas antimalaria secara in vitro terhadap P. falciparum strain 3D7 (sensitif klorokuin) dengan IC50 1,8 µg/mL dan terhadap strain K1 (resisten klorokuin) dengan IC50 3,3 µg/mL. Senyawa (2) dan (3) menunjukkan aktivitas antikanker secara in vitro terhadap sel kanker payurada T47D dengan IC50 masing-masing 3,0 dan 3,2 µg/mL. Tumbuhan E. variegata mempunyai potensi sebagai bahan dasar obat herbal antikanker payudara dan antimalaria.
SELAGINELLA ACTIVE FRACTIONS INDUCE APOPTOSIS ON T47D BREAST CANCER CELL Handayani, Sri; Risdian, Chandra; Meiyanto, Edy; Udin, Zalinar; Andriyani, Rina; Angelina, Marissa
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.839 KB) | DOI: 10.14499/indonesianjpharm0iss0pp48-53

Abstract

Apoptosis  is  an  important  target  on  anticancer  mechanism. The  purpose  of  this  research  is  to  investigate  apoptosis  induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol  was  used  to  extract Selaginella  plana powders.  Ethanolic extract  was  dilluted  by  methanol:water  (4:1)  and  then fractionated  by  hexane  (S_Hex),  methylene  chloride  (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D  cell  line  was  detected  by  SRB  (Sulforhodamine  B) assay which  was  measured  at  a  wavelength  of  515nm.  Flowcytometry analysis  to  determine  apoptosis  was  examined  by  Propidium Iodide  (PI)  and  Annexin  V  assay  using  T47D  breast  cancer  cell line.  The  result  showed  that  the  IC50 value  of  S_Hex,  S_MTC, S_EA,  and  S_BuOH  on  T47D  cells  were  107  µg/mL,  4  µg/mL,  6 µg/mL,  and  17  µg/mL  respectively.  The  active  fractions  (S_MTC and  S_EA)  at  its  IC50 concentration  significantly  (P<0.05) increased  the  total  number  of  early  apoptotic  cells  in  the  T47D cells  3.39%  and  4.1%  respectively  compared  to  that  of  control (1.95%).  Based  on  the  result,  methylene  chloride  and  ethyl acetate  fraction  of Selaginella  plana induced  apoptosis  on  T47D cell.Keywords: apoptosis, breast cancer, Selaginella
Brazilein in combination with cisplatin inhibit proliferation and migration on highly metastatic cancer cells, 4T1 Handayani, Sri; Susidarti, Ratna Asmah; Udin, Zalinar; Meiyanto, Edy; Jenie, Riris Istighfari
Indonesian Journal of Biotechnology Vol 21, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.26106

Abstract

Brazilein performs anti­cancer activities on several cancer cells and potentially inhibits metastasis. The aims of this study is to observe the synergistic cytotoxic and migration inhibitory effect of brazilein combined with cisplatin on 4T1 breast cancer cells. Under MTT assay, we found that brazilein revealed cytotoxic effect on 4T1 cells in a dose­dependent manner (IC50=50 ± 0.3 µM). Combination of brazilein and cisplatin showed synergistic effect (CI=0.72). Flowcytometry analysis on the cell cycle progression showed that single treatment of 25 µM brazilein induced G2/M­phase accumulation, 12.5 µM cisplatin induced S­phase accumulation, while combination of brazilein and cisplatin induced S­phase and G2/Mphase accumulation. Combination of brazilein and cisplatin induced apoptosis higher than that of the single treatments. Based on wound healing assay, 12.5 µM brazilein and its combination with 6.25 µM cisplatin inhibited cells migration. Immunoblotting and gelatin zymography analysis showed that combination of brazilein and cisplatin inhibited the expression level of Rac1 and MMP9 proteins. Based on these results, we conclude that brazilein enhanced cytotoxic activity of cisplatin and inhibited migration on 4T1 cells and potentially can be developed as an enhancing cytotoxic and antimetastasis agent.
ANTICANCER ACTIVITY OF ETHANOLIC EXTRACT OF Selaginella plana HIERON. ON T47D CELL LINE IN VITRO Risdian, Chandra; Handayani, Sri; Udin, Zalinar; Andriyani, Rina; Angelina, Marissa
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v13i2.153

Abstract

Selaginella sp belongs to the Selaginellaceae family. It has been used in China and Indonesia as a traditional medicine. It hasseveral medicinal properties including antibacterial, anticardiovascular, and anticancer agent. The aim of the present study was to access the anticancer property of the ethanolic extracts of Selaginella plana Hieron. on T47D breast cancer cell line. The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515 nM. The result showed that the IC50 of the ethanolic extract was determined at 7.03 pg/mL. This significant activity was assumed due to its high total flavonoid content. The totalflavonoid content of the ethanolic extract was 23.04%. Flow cytometry analysis indicated that the extract may undergo the cell death via apoptosis pathway. In conclusion, the ethanolic extract of Selaginella plana Hieron: has considerable activity in inhibiting T47D cell line proliferation.Key words: Selaginella plana Hieron., SulforhodamineB, T47Dcells, anticancer flow cytometry
SITOTOKSISITAS XANTHORRHIZOL dari MINYAK ATSIRI RIMPANG Curcuma xanthorrhizaRoxb. terhadap SEL KANKER PAYUDARA YBM-1 Udin, Zalinar
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 15, No 1 (2013)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v15i1.101

Abstract

Curcuma xanthorrhiza Roxb. (temulawak) known hascytotoxic activity against cancel cells. The active compoundofCurcuma xanthorrhizaRoxb., for several cancer cells isxanthorrhizole. This research would assay the cytotoxicactivity of volatile oil from Curcuma xanthorrhizaRoxb. 'srhizome toward YMB-1 breast cancer cell. The method wasdone by in-vitro with alamar blue dyed, and measuredtheabsorbance to observe the viability (%) of cell and IC,ovalue. Xanthorrhizole used in this research was isolate fromethyl acetate fraction of Curcuma xanthorrhizaRoxb. 'srhizome compared the result with pure xanthorrhizol . Thecytotoxic activity assay toward YMB-1 cell showed thatxantorrhizole has highest activity with ICso 2.88 glmL,followed by the volatile oil with IC,o 3.20 glmL.lt meansthatactivity of volatile oil was not significantly different comparedwith that of xanthorrhizole . The lowest activity was found onisolate C27 with ICso 4.80 g/ml, while antimycin as positivecontrol gave IC,o 1.03 glmL..Keywords: Curcumaxanthorrhiza Roxb., volatile oil,xanthorrhizol,breast cancer,YMB-1cell, alamar blue method
Cytotoxic and Antimetastasis Effect of Ethyl Acetate Fraction from Caesalpinia sappan L. on MCF-7/HER2 Cells Jenie, Riris Istighfari; Handayani, Sri; Susidarti, Ratna Asmah; Udin, Zalinar; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 8, No 1 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (781.574 KB) | DOI: 10.14499/indonesianjcanchemoprev8iss1pp42-50

Abstract

Overexpression of HER2 in breast cancer cell is found on invasive breast cancer and correlated with worse prognosis. Caesalpinia sappan L shows cytotoxic activity on various cancer cells. The goal of this research is to determine the cytotoxic activity and inhibition of migration and invasion of ethyl acetate fraction of Caesalpinia sappan L. (FEA) on HER2 overexpression-breast cancer cells (MCF-7/HER2). The MTT and flow cytometry assay showed that FEA revealed cytotoxic effect in a dose-dependent manner (IC50= 34 ± 3.1 µg/ml) and induced apoptosis, S and G2/M phase accumulation. Wound healing assay, gelatin zymography and immunoblotting assay showed that FEA inhibited migration and suppressed MMP2, MMP9, HER2 and Rac1 protein level. Thus, ethyl acetate fraction of Caesalpinia sappan L. is potential to be developed on future research especially to treat metastatic breast cancer with HER2 overexpression.Keywords: Ethyl acetate fraction of Caesalpinia sappan L, cytotoxic effect, migration and invasion, MCF-7/HER2 cells
Molecular Docking of Robustaflavone Derivatives on Pi3k Protein Target and The Correlation With Its Cytotoxic Activity on Cancer Cells Handayani, Sri; Udin, Zalinar
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (827.932 KB) | DOI: 10.14499/indonesianjcanchemoprev2iss3pp319-324

Abstract

Robustaflavone derivatives have cytotoxic effect on cancer cell. Inhibition of PI3K on cancer cell has correlation with apoptotic induction. The aim of this research is to observe interaction from robustaflavone derivatives on PI3K receptor that may give contribution to the cytotoxic effect of the compounds on cancer cell line. Geometric optimization of robustaflavone derivatives structured was done used Hyperchem 7.5 software. Arguslab 4.01 software with GAdock method applied for the docking step. The docking result showed that robustaflavone 7,4',7''- trimethyl ether (RTE) has affinity on PI3K target receptor which gave the lowest Gibbs free energy (∆G -10,855 kcal/mol).Keywords: robustaflavone, PI3K, cancer, docking