Reiny A Tumbol
Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi

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Evaluation of Baker’s Yeast (Saccharomyces cereviciae) In Enhancing Non Specific Immune Response and Growth of Nile Tilapia Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : e-Journal BUDIDAYA PERAIRAN

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This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus
DETERMINASI MOLEKULER KOI HERPES VIRUS (KHV) YANG DIISOLASI DARI IKAN KOI (Cyprinus carpio koi) Saselah, Jetti Treslah; Tumbol, Reiny A; Manoppo, Henky
JURNAL PERIKANAN DAN KELAUTAN TROPIS Vol 8, No 2 (2012)
Publisher : Sam Ratulangi University

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Penelitian ini bertujuan untuk mendeterminasi keberadaan Koi Herpes Virus (KHV) pada ikan koi (Cyprinus carpio koi). Sampel ikan diambil dari Kabupaten Kepulauan Sangihe. Penelitian di lakukan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi dengan menggunakan metode Polymerase Chain Reaction (PCR). Hasil pemeriksaan PCR terhadap sampel ikan koi mengindikasikan bahwa sampel ikan koi telah terinfeksi oleh virus KHV, yang ditandai dengan munculnya pita DNA pada hasil visualisasi elektroforesis agarosa. Hasil pemeriksaan pada morfologi ternyata sampel ikan yang digunakan menunjukkan gejala-gejala klinis terserang KHV seperti mata pucat, insang berwarna pucat serta produksi lendir yang berlebihan. Beberapa sampel lainnya walaupun secara morfologi belum menunjukan gejala-gejala klinis tetapi melalui pemeriksaan PCR telah terindikasikan terinfeksi KHV. Kata kunci: KHV, PCR, ikan koi   The purpose of this study was to determine the presence of Koi Herpes Virus (KHV) in Koi (Cyprinus carpio koi). Fish samples were taken from the Sangihe Archipelago Regency. Laboratory work conducted at the Laboratory of Biology Faculty of Mathematics and Natural Sciences University of Sam Ratulangi using Polymerase Chain Reaction (PCR) method. The samples indicated that the koi fish samples were infected with KHV. It is shown by the appearance of DNA bands on agarose electrophore­sis visualization. The morphological examination of the body indicated clinical symptoms of KHV infection, such as pale eyes, pale gills and excessive mucus production. Several other samples, although without clinical symptoms, have shown to be infected as indicated by PCR test. Keywords: KHV, PCR, Koi.
The use of β-glucan extracted from baker’s yeast (Saccharomyces cerevisiae) to increase non-specific immune system and resistence of tilapia (Oreochromis niloticus) to Aeromonas hydrophila Jamal, Ida N; Tumbol, Reiny A; Mangindaan, Remy E.P
AQUATIC SCIENCE & MANAGEMENT (Jurnal Ilmu dan Manajemen Perairan) Edisi Khusus 1 (2013): Mei
Publisher : Pascasarjana, Universitas Sam Ratulangi

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Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from bakers yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©
Kelayakan lokasi budidaya ikan di Danau Tondano ditinjau dari parameter fisika kimia air Kamsuri, Agus I; Pangemanan, Penky N.L; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 3 (2013)
Publisher : e-Journal BUDIDAYA PERAIRAN

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The purpose of this study was to determine the current condition of the water quality of Lake Tondano in terms of physical and chemical parameters in a fish farming locations on Lake Tondano waters. Determination of sampling points at each station is placed vertically at three predetermined points from the guard house toward the front of the net, the distance between one point to the next point was ± 10 m; whereas for the analysis of water quality parameters was done in Clinical Pathology of Fish Diseases Laboratory, Faculty of Fisheries and Marine Science, Sam Ratulangi Manado and Manado Industrial Research and Standards Laboratory. Determination points were done by purposive sampling which refers to the physiographic location wherever possible in order to represent or describe these waters. The research was carried out for 6 weeks and was done in 2 stages, morning and afternoon. For direct measurement (in situ) was performed once a week which included parameters DO, pH, temperature, and brightness, while the laboratory tests were conducted for 2 weeks which included parameters Nitrite, Nitrate, Iron, Chlorine, Manganese, Chloride, Sulfate, Aluminum, ammonia and Phosphate. The results show that the locations of the four parameters of chlorine (CL2) with range (0.1 to 0.28) and the parameters of Ammonia with the range (0.0125 to 0.15) over the limit indicated on water quality standards. The parameters of temperature, DO, pH, brightness, nitrate, nitrite, sulfate, manganese, chloride, aluminum, iron and phosphate were still in the water quality standard PP No.82 of 2001.
Detection of the presence Aeromonas sp on nile tilapia cultured in floating net cage in lake Tondano Tantu, Wandalia; Tumbol, Reiny A; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 1, No 3 (2013)
Publisher : e-Journal BUDIDAYA PERAIRAN

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The purpose of this study was to detect the presence of bacteria Aeromonas sp in tilapia (Oreochromis niloticus). Nine fish with an average body weight of 150-200 g were used in this study as samples. Sampling was conducted at three different locations which were representative of fish culture sites located on Lake Tondano. The number of sample gathered from each location was and 3 fish which were taken randomly. Fish samples were taken by using a scoop, then the samples were taken alive by placing in an oxygen-filled plastic separately and taken direcly to lab Fish Disease Control and Environmental Center, Tateli, Department of Marine and Fisheries of North Sulawesi province, for examination the presence of bacteria. Isolation of bacteria were conducted by taking samples from gill and kidney. This study was carried out from April - July 2013. Identification of the presence of bacteria was done through a series of observations of colony morphology and gram staining of bacteria, followed by a series of biochemical tests: oxidase test , catalase test , test TSIA , H2S production test , indole test , motility test , citrate test, and test O/F. It could be concluded that 22.22 % of farmed nile tilapia in Lake Tondano were infected with the Aeromonas sp with the following percentage from each site: Paleloan village 7.40% , 7.40% Toulimembet village, and 7.40% Eris village. Keywords: Aeromonas sp, isolation, nile tilapia, floating net cage, Lake Tondano
Diagnosa Penyakit Bakterial Pada Ikan Nila (Oreocrhomis niloticus) Yang Di Budi Daya Pada Jaring Tancap Di Danau Tondano Ashari, Chairanitansyah; Tumbol, Reiny A; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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This research aimed to diagnose bacterial diseases on nile tilapia cultured in stick net cage in Lake Tondano. Fish samples were taken from three locations of net cages in Lake Tondano namely Paleloan, Eris and Remboken villages. Three samples of fish were taken from each location/village. The samples taken showed clinical symptoms of bacterial infection such as hemorrhagic on the abdomen, the base of the fin, anal and chest, irregular swimming fish, hemorrhagic on the gills, fish refuse food, remain silent on the outskirts of net cage. Bacteria were isolated by using a sterile loop from head kidney (anterior kidney), which is located on the front, below the spine near the head. Bacterial cultures were performed on TSA media by making a zigzag scratches. The petri dish containing bacterial isolates were subsequently incubated in an incubator at a temperature of 35oC for 24 hours. The water quality parameter data measured consisted of temperature, Dissolved Oxygen, pH, ammonia, brightness. The results of the study showed that farmed tilapia in Lake Tondano has been infected with Aeromonas hydrophila that causes MAS (Motile Aeromonad Septicemia). The water quality, both chemical and physics around the net cages in the Lake Tondano was still in good condition and suitable for fish cultivation.   Keywords: Oreocrhomis niloticus, Bacterial disease, septicemia aeromonad motile, fixed net cage, Lake Tondano
Identifikasi Penyakit Bakterial Pada Benih Sidat (Anguilla marmorata) di Balai Budidaya Air Tawar Tatelu Kusen, Kevin Octavian; Tumbol, Reiny A; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

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This study aimed to identify the bacterial disease in cultured eel elvers (Anguilla marmorata). The samples used for this study were taken from the Tatelu Freshwater Aquaculture Center. The samples taken were elvers (juvenile life stage of A. marmorata)  which showed clinical symptoms of bacterial infection such as bleeding in the chest, abdomen, and the base of the fin, passive movement, weakness, and loss of balance of the body, loss of appetite, some wounds on back fin, chest, and tail, and the body was not slippery/shiny. Three elvers with the size of 4-6 cm, and weigh about 0.2 g were taken as samples. The organ chosen to be targeted for isolation was head kidney. Each sample was isolated twice as replicates and streaked on Triptic Soy Agar (TSA) medium. First replicates were identified in the Tatelu Health Lab of Freshwater Aquaculture, and the second replicates were identified in North Sulawesi Provincial Health Lab. Bacterial identification was done through a series of observations and morphology of bacterial colonies through gram stain, followed by biochemical tests through oxidase test, catalase test, Triple Sugar Iron Agar (TSIA) test, H2S production test, motility test, O/F test, and Simmons Citrate Agar (SCA) test. Water quality parameters that were measured included temperature, disolve oxygen, pH, and ammonia. Based on identification of bacteria in cultured elvers in Tatelu Freshwater Aquaculture Center, the elver samples had been infected with bacterial  Aeromonas hydrophila. Keywords: elver, identification, Aeromonas hydrophila, Tatelu Freshwater Aquaculture Center
Penambahan bakasang pada pakan benih sidat (Anguilla marmorata) untuk meningkatkan sistem imun non spesifik Pinoke, Steven A.J; Tumbol, Reiny A; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 3, No 3 (2015)
Publisher : e-Journal BUDIDAYA PERAIRAN

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This study was aimed to examine the effect of immunostimulant bakasang (fermented product from fish) and to determine the optimal dose of bakasang in increasing the non-specific immune response of eels. The eels were obtained from Institute of Freshwater Aquaculture (BPBAT) Tatelu. Bakasang as immunostimulant ingredient contained Lactic Acid Bacteria (LAB) with the bacteria concentration of 105 CFU/ml was prepared by diluting with clean water to get the doses desired. The feed was subsequently mixed with the commercial fish feed in powder form and mixed well with water to form paste. This research used Two-ways ANOVA with 4 different types of treatments A (0 ml/kg feed),   B (50 ml/kg feed), C (100 ml/kg feed), D (150 ml/kg feed) and 3 repetitions. The fish were feed with treatments diets for 3 weeks with a dose of 3% body weight per day with a frequency of twice a day (at 08:00 am) and the afternoon (16.00 pm). The parameters measured was Total Leukocyte Count (TLC) which was measured every week (t0, t1, t2, t3). The results indicated that the addition of bakasang on eels’feed gave a significant increased on TLC, and that the highest TLC was found in the dose of 100 ml/kg feed. This shows that the optimal dose of bakasang in increasing the TLC of eels  was 100 ml/kg feed after administration of 3 weeks. Keywords: Eels, Bakasang, Total Leucocytes Count
IDENTIFIKASI KOI HERVES VIRUS PADA IKAN MAS Cyprinus carpio DI SULAWESI UTARA TAHUN 2017 DENGAN MENGGUNAKAN TEKNIK PCR DAN qPCR Sultan, Makkulau; Wullur, Stenly; Tumbol, Reiny A
JURNAL PESISIR DAN LAUT TROPIS Vol 6, No 2 (2018): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

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This research aimed to detect the distribution of KHV disease in cultured common carps using conventional PCR and Real Time Quantitative PCR methods in North Sulawesi. The samples were taken from 6 aqua culture centres in North Sulawesi. The results of KHV detection by PCR method showed negative KHV infection because visualization does not form a specific band with the KHV gene that is at 409 bp. Detection of KHV of Ct (Quantification cycle) was greater than the LOD with a confidence level of 95% where Ct LOD is 8.71 for the smallest standard of 1.0x102 copies. Ct sample that was read based on qPCR amplification result which was 14,69-18,80 and the value of Ct NTC (Non Template Control) used as a negative control was 17.52.Penelitian ini bertujuan untuk mengidentifikasi keberadaan penyakit Koi Herves Virus pada ikan mas dengan menggunakan metode PCR dan qPCR di Sulawesi Utara. Sampel uji diambil dari 6 sentral budidaya di Provinsi Sulawesi Utara. Hasil penelitian menunjukkan bahwa dengan metode PCR diperoleh hasil deteksi yang negatif, karena visualisasi tidak terbentuk band spesifik dengan KHV, yaitu di 409 bp. Deteksi KHV dengan metode qPCR didapat hasil infeksi KHV yang negatif dilihat dari nilai rata-rata Ct (Quantification cycle) lebih besar dari LOD dengan tingkat kepercayaan (confident level) 95%. Nilai Ct LOD adalah 8,71 untuk standar terkecil 1,0x102 copies, sedangkan Ct sampel hasil amplifikasi qPCR adalah 14,69-18,80 dan nilai Ct NTC (Non Template Control) yang digunakan sebagai kontrol negatif adalah 17,52.