Cenra Intan Hartuti Tuharea
Unknown Affiliation

Published : 1 Documents

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Pollen Viability and Pollen Tube Growth of IPB’s Papaya Suketi, Ketty; Tuharea, Cenra Intan Hartuti; Widodo, Winarso Dradjad; Poerwanto, Roedhy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 1 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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The purpose of the research  was to examine the pollen germination process and growth rate of pollen tubes of papaya. Pollen tube growth of nine genotypes of papaya (IPB 1, IPB 2, IPB 3, IPB 4,  IPB 5, IPB 7, IPB 8,  IPB  9,  and  IPB 10) was investigated in this experiment in order to study their pollen germination rate and pollen viability. The fresh pollen were excised from the flowers of papaya grown at Tajur Field Station of  Research Center for Tropical Fruit, Bogor Agricultural University (IPB), Bogor. The extracted pollen was cultured aseptically on the Brewbaker and Kwack medium (pH 7.3) at ambient  temperature of 26-28 °C. Pollen germination and pollen tube growth was observed under optical microscope with 100 and 400 magnification. Papaya pollen viability was not associated with size-based categories of papaya fruits. IPB 4 had the longest pollen tube at first 30 minutes after germination  (115.5 µm), whereas IPB 10 had the shortest (99.5 µm).  The distance  from  stigma  to   ovary  in  hermaphrodite  flowers  varies with genotypes, ranging from 7.38 to 13.44 mm.  Average length of pollen tube within four hours of germination for small papaya fruit category (IPB 1, IPB 3, and IPB 4) was 1,030.67 ± 19.14 #m, while the distance between stigma and ovary was short (14.85 ± 2.19 mm) so that the expected of  fertilization process occurred sooner.  At the end of the experiment (four hours after germination), IPB 1 genotype  had the longest pollen tube (1,052 µm) while IPB 9 genotype (913 µm) had the shortest  pollen tube.  Genotype with the highest percentage of germination  at the end experiment was IPB 2 (65.65%), whereas the lowest was IPB 7 (42.56%).   Keywords: Carica papaya, germination rate, pollen germination