Triyuliati Triyuliati
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Peran Limfosit T Helper-1 (TH1) dan T Helper-2 (TH2) pada Patogenesis Artritis Lepra Hadi, Suyanto; Sunarto, Sunarto; S, Hardyanto; Triyuliati, Triyuliati; J, Susanto; Hartono, F.X.
MEDIA MEDIKA INDONESIANA 2008:MMI Volume 43 Issue 1 Year 2008
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTThe role of lymphocyte T Helper-1 (TH1) and T Helper-2 (TH2) in the pathogenesis of leprosy arthritisBackground: The autoreactive of TH CD4+ cells is the thought to play an important role in arthritis leprosy pathogenesis. However, wheter of TH1 or TH2 predominant has never been studied.Methods: Various Ag M. leprae (Ag 35 kDa, 10 kDa, 45 kDa, 85 kDa, and MLSA 2 ug/ml) were stimulated to the peripheral blood (10cc) lymphocyte culture (PBMC) using 96 weels microplate and RPMI 1640 media of 22 leprosy arthritis cases, control-1 (n=12) (leprosy without arthritis) and control 2 (n=12) (healthy contact). The activity difference between TH1 and TH2 CD4+ lymphocyte was analysed using the difference delta levels of IFN-γ and IL-4 (ELISA) of the three group studies. Statistical analysis used wereANOVA, Kruskal Wallis or Mann Whitney, and Chi-square.Results: IFN-γ delta levels was significantly higher in the lymphocytes cultures in LA group (the median 132.234 pg/ml, 60.347 g/ml, 14.093 pg/ml, 16.619 pg/ml and 138.394 pg/ml) compared with IL-4 value level (median 0.317 pg/ml, 0.017 pg/ml, -0.206 pg/ml, -0.200 pg/ml and 0.492 pg/ml) after being stimulated with 35 kDa, 10 kDa MMP-1, 45 kDa LAM, 85 kDa and MLSA of 2 ug/ml dose consecutively (all p<0.001). The IFN-γ delta value in LA group also showed the significantly higher level in response toall M. leprae Ag compared to all control-groups, with all p value < 0.05.Conclusion: TH1 CD4+ lymphocyte activity is more dominant compared than TH2 CD4+ lymphocyte activity in leprosy arthritis group patients.Key Words: Leprosy arthritis, IFN-γ, IL-4, TH1 CD4+ and TH2 CD4+ lymphocyte, and M. leprae Ag ABSTRAKLatar belakang: TH CD4+ autoreactive diduga kuat pada patogenesis artritis lepra. Apakah autoimunitas akibat dominansi aktivasi limfosit TH1 atau TH2 pada penderita lepra belum pernah diteliti.Metode: Berbagai Ag M.leprae (35 kDa, 10 kDa, 45 kDa LAM, 85 kDa, dan MLSA dosis 2 ug/ml) distimulasikan pada kultur limfosit darah perifer (10 cc darah vena), menggunakan media RPMI 1640 dari 22 kasus artritis lepra, 12 kontrol-1 (lepra tanpa artritis), dan 12 kontrol-2 (tetangga sehat kontak positif). Nilai delta kadar IFN-γ dan IL-4 (ELISA) diukur untuk mengetahui peran aktivitas limfosit TH1 dan TH2. Statistik yang dipergunakan uji ANOVA, uji Kruskal-Wallis atau Mann Whitney, dan metode Chi-square.Hasil: Nilai delta IFN-γ kultur limfosit kasus artritis lepra (median 132,234 pg/ml, 60,347 pg/ml, 14,093 pg/ml, 16,619 pg/ml dan 138,394 pg/ml) kelompok LA lebih tinggi bermakna dibandingkan nilai delta IL-4 (median 0,317 pg/ml, 0,017 pg/ml, -0,206 pg/ml, - 0,200 pg/ml dan 0,492 pg/ml) pasca stimulasi dengan Ag M. leprae 35 kDa, 10 kDa MMP-1, 45 kDa LAM, 85 kDa dan MLSA dosis 2 ug/ml, (p<0,001). Nilai delta IFN-γ kelompok kasus juga lebih tinggi dibandingkan kedua kelompok kontrol (p< 0,05).Simpulan: Aktivitas limfosit TH1 CD4+ lebih dominan dibandingkan TH2CD4+ pada kelompok penderita artritis lepra.
Kadar IFN-γ (fungsi TH-1 CD4+) dan IL-4 (Fungsi TH-2 CD4+) pada Penderita Artritis Lepra Hadi, Suyanto; Triyuliati, Triyuliati
MEDIA MEDIKA INDONESIANA 2010:MMI VOLUME 44 ISSUE 1 YEAR 2010
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

The correlation between IFN-γ (TH-1 CD4+ function) and IL-4 level (TH-2 CD4+ function) in leprosy arthritisBackground: It was hypothesized by some expertise that there is a negative correlation between TH-1 CD4+ function (IFN- level) and TH-2 CD4+ function (IL-4 level) in rheumatoid arthritis and lupus pathogenesis (Leader). The correlation between IFN-γ level and IL-4 level in the leprosy arthritis has never been reported. The objective of the study is to evaluate the correlate between IFN- and IL-4 level in leprosy arthritis. Method: The correlation between IFN- level (pg/ml) and IL-4 level (pg/ml) were analysed after stimulated with several M. leprae Ag. (10 kDa, 35 kDa, and MLSA 2 ug/ml) on the peripheral blood lymphocyte culture of leprosy arthritis patients (n=22) (RPMI 1640 sigma). The study was carried out at Hayati Laboratory Gajah Mada University. The Spearman correlation test was used in analysis.Result: There were no significant correlations between IFN- and IL-4 level given 3 different numbers of M. leprae with r1=-0.32, r2=0.262, and r3=0.324 with p1=0.888, p2=0.238, and p3=0.747 respectively of Spearman test on arthritis leprosy group. Conclusion: There is no correlation between IFN- and IL-4 level in leprosy arthritis patients.ABSTRAKLatar belakang: Dihipotesiskan oleh para ahli adanya korelasi negatif antara fungsi limfosit TH1CD4+ (kadar IFN- pg/ml) dan limfosit TH-2 CD4+ (kadar IL-4 pg/ml) pada artritis reumatoid dan lupus eritematosus sistemik (Leader). Korelasi kadar IFN-γ (pg/ml) dan kadar IL-4 (pg/ml) pada artritis lepra belum pernah dilaporkan. Penelitian ini bertujuan untuk mengevaluasi korelasi antara kadar IFN- dan IL-4 pada penderita artritis lepra.Metode: Metode penelitian yang dipergunakan adalah belah lintang. Dilakukan analisis hubungan kadar IFN- (pg/ml) dan IL-4 (pg/ml) pada kultur limfosit darah perifer (RPMI 1640 sigma) penderita artritis lepra pasca stimulasi beberapa Ag M. leprae (10 kDa, 35 kDa, dan MLSA 2 ug/ml). Kultur limfosit darah dilakukan di Laboratorium Hayati FK UGM Yogyakarta. Dilakukan uji korelasi dengan Spearman. Hasil: Tidak terdapat korelasi antara kadar IFN- (pg/ml) dengan kadar IL-4 (pg/ml) setelah distimulasi dengan dosis berbeda, dengan nilai r1=-0,32, r2=0,262, dan r3=0,324 berturut-turut disertai nilai p1=0,888, p2=0,238, dan p3=0,747 dengan uji korelasi Spearman. Simpulan: Tidak terdapat korelasi antara kadar IFN-γ dengan IL-4 pada kultur limfosit darah perifer penderita artritis lepra