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Journal : Jurnal Perikanan Universitas Gadjah Mada

EFEKTIVITAS VAKSIN POLIVALEN UNTUK PENGENDALIAN VIBRIOSIS PADA KERAPU TIKUS (Cromileptes altivelis) Nitimulyo, Kamiso Handoyo; Isnansetyo, Alim; Triyanto, Triyanto; Murdjani, Muhammad; Sholichah, Lili
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9056

Abstract

The objective of this research was to know the effectiveness of polyvalen  Vibrio vaccine to control vibriosis in humpback grouper (Cromileptes altivelis). The effectiveness of vaccine was evaluated by the survival rate (SR), relative percent survival (RPS), mean time to death (MTD) as well as growth rate of vaccinated fish. This research consisted of 4 treatments (control, injection, immersion, and oral vaccinations) in quadruplicates. Injection  vaccination was conducted by intraperitoneal injection of polyvalen vaccine at 107 cells/fish. Immersion vaccination was done by immersing the fishes at 107 cells/ml for 30 minutes. Oral administration of vaccine was also carried out  at 107 cells/fish. One week after the first vaccination, second vaccination (booster) was carried out at the same dosage and by the same administration. One week after the second vaccination, fishes were challenged with 3.16x104 cells/fish of Vibrio ordalii 3J by intraperitoneal injection, and reared for 20 days post infection. Results indicated that polyvalen Vibrio vaccine increased SR (P<0.01) up to 100%. Vaccination was also able to delay MTD of fishes. However, the vaccination was not influence the growth rate of fish.
PENGARUH TETRAKSIKLIN TERHADAP KERENTANAN OLEH SERANGAN PENYAKIT MAS, PERTUMBUHAN DAN DAYA TETAS TELUR LELE DUMBO (Clarias gariepinus) N., Kamiso H.; Triyanto, Triyanto
Jurnal Perikanan Universitas Gadjah Mada Vol 1, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8855

Abstract

Study was conducted to find out the effect of tetracycline medication to suspectibility against MAS, growth rate and hatching rate of African catfish (Clarias gariepinus). The result indicated that intraperitoneal injection of tetracycline (50 mg/kg fish) could decrease the growth rate of fish significantly (P<0.05). However, this medication did not have significant effect to suspectibility of fish against A. hydrophila the causative agent of MAS. Intraperitoneal injection of tetracycline (50 mg/kg fish) in african catfish brooders two weeks before spawning also did not effect fertility or hatching rate of eggs.  
IDENTIFIKASI BAKTERI PATOGEN PADA KUDA LAUT (Hippocampus kuda) DI BALAI BUDIDAYA LAUT, LAMPUNG Adiputra, Yudha Trinoegraha; Triyanto, Triyanto; Probosunu, Namastra
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9064

Abstract

Sea horse (Hippocampus kuda) is one of the ornamental marine organisms and raw material of traditional medicine. Since 1993, Seafarming Development Centre, Lampung has pioneered a research and  culture  of sea horse in Indonesia. The serious problem in the culture of sea horse is pathogenic bacteria caused death of juveniles and broodstocks. The objective of this study was to identify pathogenic bacteria isolated from  sea horse in Seafarming Development Centre. Koch Postulate test was carried out, and then the pathogenic bacteria were identifed by morphological and biochemical tests. Results showed that from a total of 6 bacterial strains isolated from diseased sea horse, 3 strains were pathogenic bacteria to sea horse. These 3 pathogenic bacteria caused identical disease signs with the initial disease signs when the bacteria were isolated.  Morphological and biochemical tests suggested that  the pathogenic bacteria could be identified to be Vibrio fluvialis, V. alginolyticus and V. hollisae. 
VAKSINASI INDUK LELE DUMBO (Clarias gariepinus) UNTUK MENINGKATKAN KELULUSHIDUPAN DAN PERTUMBUHAN PADA TAHAP PENDEDERAN Triyanto, Triyanto; N., Kamiso H.; Isnansetyo, A.
Jurnal Perikanan Universitas Gadjah Mada Vol 1, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8857

Abstract

Female and male brooders were vaccinated by intraperitoneal injection and intramuscular injection respectively. Unvaccinated brooders were used as control. Fry which was produced by vaccinated and unvaccinated brooders were reared separately in paddy field pond for 15 days for the first nursery rearing period and another 15 days for the second nursery rearing period. These field experiment conducted in four locations, namely slmean (2 location) and magelang (2 location). Results of these experiments indicated that brooders vaccination could increase survival rates of the first and the second nursery rearing periods approximately 65.00% and 3.69% respectively. Average weight and total length of fry which produced by vaccinated broodstock increased 12.59% and 58.51% during the first nursey rearing period, 8.55% and 7.14% during the second nursery period.
PENGARUH VAKSINASI INDUK LELE DUMBO (Clarias gariepinus) TERHADAP KELULUSHIDUPAN, PERTUMBUHAN BENIH DAN PRODUKSI IKAN Triyanto, Triyanto; N., Kamiso H.; Isnansetyo, A.
Jurnal Perikanan Universitas Gadjah Mada Vol 1, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8848

Abstract

In Indonesia, outbreak of MAS on cultured African catfish  occurs periodically. The outbreek seems relate closely to quality of fry,. High quality fry and proper handling will ensure the successful of African catfish culture. Vaccination on vrooders is one of methods to produce high quality of African catfish fry. The objectives of this experiment  were to know effect of fingerling produced by vaccination brooders on survival., growth rate, and fish production  during grow out periode. Female and male brooders were vaccinated by intraperitoneal and intramuscular injection of 0.5 ml vaccine respectively. Unvaccinated brooders were used as control. Those brooders were spawned 2-4 weeks after vaccination. Fry which produced by unvaccinated brooders was reared separately in paddy field pond 15 days for the first nursery rearing. Fingerling produced by the second nursery rearing period was used for grow out for 3 mounths. Results of this experiment indicated that brooders vaccination could increase survival rate and production of African catfish approximately 22.16% and 9.94% from control respectively. However, the length and weight of fingerling produced by vaccinated brooders were lower than fingerling produced by unvaccinated brooders
Patogenisitas Vibrio Fluvialis 24SK terhadap Kerapu Tikus (Cromileptes altivelis) Istiqomah, Indah; Isnansetyo, Alim; Triyanto, Triyanto; Nitimulyo, Kamiso Handoyo; Murdjani, Muhammad
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.159

Abstract

This research was aimed to investigate the pathogenicity of Vibrio fluvialis 24SK in humpback grouper (Cromileptes altivelis) based on its Lethal Dosage 50 (LD50). V. fluvialis 24SK was isolated from ren of humpback grouper cultured in floating net cage at Brackishwater Aquaculture Development Center (BADC) Situbondo, with vibriosis signs. The bacterium was cultured in Tryptone Soy Broth (TSB) medium dissolved in trisalt solution (KCl, 0.75 g/l; MgSO4.7H2O; 14.2 g/l; NaCl, 18.4 g/l), incubated at 300C for 24 h. Infection was carried out by interperitoneal injection to humpback grouper (8-9 cm of total length) at 102, 104, 106, and 108 cfu/fish. Control fishes were injected with 0.2 ml trisalt solution. Disease sign and mortality of fishes were observed every eight hour for 40 days. LD50 was calculated based on Dragstedt Behrens method (Hubert, 1980). Result indicated that infection of the bacteria at 106 and 108 cfu/fish caused sub-acute disease signs, such as haemorhagic on operculum, base of fins (pinnae pectorales, pinnae abdominales, pinna analis), and also head and abdomen, while infection at 102 and 104 cfu/fish caused chronic disease signs, such as haemorhagic on fins base which was followed by necrotic on fins and skin tissue in prolonged time. Histopathologically, infection of the bacteria caused atrophy on the gills, infiltrations of lymphocyte, heterofel and plasma cell on the gills and fins base, vacuolar degeneration on the liver, and also present the bacteria colony on the fins base and intestine tissues. V. fluvialis 24SK has LD50 at (1,1±0,5)x107 cfu/fish.
PERBEDAAN DAYA TAHAN NON SPESIFIK LIMA SPESIES IKAN AIR TAWAR TERHADAP INFEKSI Aeromonashydrophila Syakuri, H.; Triyanto, Triyanto; Nitimulyo, K. H.
Jurnal Perikanan Universitas Gadjah Mada Vol 5, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9031

Abstract

Fish naturally has a non-specific defense against several pathogens. The non-specific defense is anatomical and physiological function that varies according to genetical and environmental factor. The differences of non-specific defense against Aeromonas hydrophila infection were studied in blackfinpacu (Colossoma macropomum), gourami (Osphronemus goramy), common crap (Cyprinus carpio), African catfish (Clarias gariepinus), and red nile tilapia (Orochromis sp.). This study also examined the differences on several parameters of non-specific defense, including differentiation anc number of leucocytes, serum total, titer of antibody, and antibacterial activity of skin mucus.The fishes were intramuscularly infected at the media lethal doses for common carp (7,4 x 108cfu/fish). Pathological changes, survival rate, and mean time to death were observed every day. The observation of non-specific defense parameters were carried out prior and at seven day after infection.The results showed thahblackfinpacu was the most resistant species against A. hydrophila infection. African catfish and red nile tilapia were most resistant than gourami and common carp. The antibody titer and percentage of thrombocytes were the causative factor for the difference of the resistence against A. hydrophila. The five fish species also showed differences on leucocytes number, monocytes and eosinophiles percentage, and serum total, but they did not showed the differences of neutrophile percentage. The skin mucus of all fish species did not exhibited antibacterial activity against A. hydrophila. This study also found increase in antibody titer and leucocytes number after A. hydrophila infection.
UJI KONSENTRASI PENGHAMBATAN MINIMAL, RESISTENSI DAN PENGGUANAAN ANTIBIOTIK UNTUK MENANGGULANGI PENYAKIT MOTIL AEROMONAS SEPTISEMIA (MAS) PADA LELE DUMBO (Clarias gariepinus) N., Kamiso H.; Triyanto, Triyanto; Hartati, Sri
Jurnal Perikanan Universitas Gadjah Mada Vol 1, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8849

Abstract

Five isolates of A. hydrophila were used for MIC, and resistency test to rifampicin, kanamycin, chloramphenicol, erythromycin and oxytetracyclin by dilution method with TSB medium. The results indicated that the bacteria had various degree of sensitivity to these antibiotics. Resistency of bacteria isolates increase 1.3 to 6.2 times after they were passaged three times to MIC of the drugs or antibiotics by dilution method. Other test was kanamycin medication conducted by i.p. injection, immersion, and oral methods. The result showed that all kanamycin medication methods were effective to decrease mortality rate caused by A. hydrophilia infection. Oral medication method was the most effective compare to other methods because this method could minimize stress during handling. However, oral medication took much longer time (15 days) than immersion and injection methods (few minutes).
ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO Nitimulyo, Kamiso Handoyo; Isnansetyo, Alim; Triyanto, Triyanto; Istiqomah, Indah; Murdjani, Muhammmad
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9053

Abstract

This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio spp., by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 106 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibriosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnisii, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on fins (pinnae pectorales, pinnae abdominales, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly.
MONITORING PARASIT PADA BUDIDAYA IKAN DAN UDANG DI DAERAH ISTIMEWA YOGYAKARTA Triyanto, Triyanto; Isnansetyo, Alim
Jurnal Perikanan Universitas Gadjah Mada Vol 6, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9041

Abstract

Parasites often cause the great loss in aquaculture either in fish or shrimp aquacultures. The loss will become more serious when fish or shrimp are secondary infected especially by bacteria. The objective of this study was to identify parasites in fish and shrimp culture inYogyakartaSpecialProvince (DIY). Samples of fish and shrimp were collected from several aquaculture centers in this region including several fish and shrimp hatcheries. Observation of parasites was conducted microscopically and macroscopically.Results showed that giant tiger prawn (Penaeus monodon) cultured in DIY was often infected by Zoothamnium sp. and Gregarines sp.. In addition, giant freshwater prawn (Machrobrachium rosenbergii) was often infected by Apiosoma sp., Dactylogyrus sp., Epystilis sp., Glossatella piscicola, Gregarines sp. and Zoothamnium sp.. Moreover Apiosoma sp.,  Dactylogyrus sp., Gyrodactylus sp., Ichthyopthirius sp., and Trichodina sp. were found in nile (Oreochromis niloticus) culture. Similar parasites such as Apiosoma sp., Dactylogyrus sp., Henneguya sp. Ichtyoptirius sp. and Trichodina sp..were found in Osphronemus gaurami. Ichtyoptirius sp. and Trichodina sp. also were found in walking catfish (Clarias gariephinus). 
Co-Authors A. Isnansetyo, A. A.E.T.H. Wahyuni Abdul Muthalib Abidin Abidin Afi Tarmiawati Agus Cahyono Agus Nursalim, Agus Agustiar, Devie Ahmad, Hariyatunnisa ALIM ISNANSETYO Ambariyanto , Andriansyah, Rico Andrianus Andrianus, Andrianus Andriyani, Fanata Anik Rahmawati, Anik Anshor, Arrini Shabrina Anung Pujiyanto Ardiyawan, Ardiyawan Arief Taslihan Arief, Ahmad Fikri Astri Charolina Azmi, Azmi Azmi, Ulya Zakaria Basri, Novysa Basuki, Umar Beniarso, Mundin Cahyana Amiruddin Ciswiyati, Dwi Endah Darfioes Basir Daya Agung Sarwono Desrina Desrina Desty Ratna Permatasari, Desty Ratna Dian Indriani Dimas A. Pamungkas Dini Siswani Mulia Dini, Tria Ayu Diniarti, Eka Drajad Sarwo Seto, Drajad Sarwo Duranta D. Kembaren, Duranta D. Dwiningtyas, Ratih Dyah Indriyani, Putri E. Sarmini Edi Irawan Eko Sugiarto, Eko Endang Sarmini Enny Lestari Erik, Mohamad Erni Martani Ervia Yudiati FAJAR SETIAWAN Fatimah Azzahrah Fatmawati, Harlinda Fauziyah, Fuzi Afiza Finny Fitry Yani Fitriani Fitriani Gunadi Gunadi H. Syakuri, H. Habib Adjie Hadi, Muhammad Tesar Hambali Hambali Hariswari, Kadek Paramitha Hartono Hartono Henny Ekana Ch. Henny Ekana Chrisnawati Herimanto Herimanto Herlina Herlina Hotnida Junita Situmeang Imamudin, Kurniasih INDAH ISTIQOMAH Irfan D. Prijambada Islami, Hidayatul Istanto, Riza Iswidiarti, Sri JAKA WIDADA Jazuli, Jazuli Juraida, Irma K. H. Nitimulyo, K. H. Kadarisman Kadarisman Kamiso H. N., Kamiso H. KAMISO HANDOYO NITIMULYO Kartikasari, Aprilia KH, Abdul Aziz Kundharu Saddhono Kurniasih Kurniasih LANGKAH SEMBIRING Lantip Diat Prasojo Lelana, Dewi Barata Siswa Lestari, Fajar Sumi Lestari, Yeni Sri Lili Sholichah, Lili Ma'sum, Aziz Malarsih Malarsih, Malarsih Manik, Astri Marita Mardi Mardi Mardiyana Mardiyana Maria Ulfahmi, Cut Gesti Mariana, Zulfa Mayang Pitaloka MIFTAKHUL JANNAH Mirwa, Tetty Muh. Ibnan Syarif, Muh. Ibnan Muhamad Aris Widodo Muhammad Murdjani Muhammad Zaini Muhammad Zainuddin Muhammmad Murdjani, Muhammmad Mujiyono Mujiyono Murti, Fahriza Murtiyoso, Onang Murwantoko Murwantoko Musa Pelu Namastra Probosunu Nina Yuliana Ningsih, Maya Nirmalasari Idha Wijaya Nisya, Hima Choirun Noer Kasanah, Noer Nur Fadhilah Nur Rokhmat Nursyirwani Nursyirwani Pratiwinindya, Ratih Ayu Prayogo, Sugeng Purwanto Purwanto Putri Puspitasari R Sudradjat, R Rachmansyah Rachmansyah Raden Bagus Sugio Sumanta Rahmawati, Dwi Tyas Ratna Puspita, Ratna Retno Widaningroem REZA Adi ADI PRADANA Ria Azizah Tri Nuraini Rini Pramesti Risca Damayanti, Risca Risnawati, Ririn Riyadi Riyadi Rohendi Rohidi, Tjetjep Rohendi Rohidi, Tjetjep Rukayah Rukayah Rumi, Jalaluddin S. Soenarjo Sabillah, Masayu Selly Safia, Rina Anggraeni Sahli, Zamahsjari Salu, Vega Ricky SANTI NURBAITI Sebastian Margino Senny Helmiati Sesung, Rusdiyanto Setiawan, Feri Setyaningrum, Heny Setyaningrum, Ikha Sulis Setyobudi Setyobudi Shanie, Arsan Shatrie, Dikry N Shatrie, Dikry Novel Siti Amirah Budiastuti Sri Hartati Sri Iswidayanti Sri Iswidayati Sri Lestari, Yeni Sri Rejeki Sriyono Sriyono Subagiyo Subagiyo Suharno Suharno Suherman Suherman Sumarwati Sumarwati Sumaryanto Florentinus, Totok Sunarhadijoso Soenarjo Sunarto Sunarto Suparti Suparti Suryanti Retna Sari, Dyah Sutarman Sutarman Sutrisno sutrisno Suyono Suyono Syafii Syafii syakir syakir Syarif, Muh Ibnan Tanjung, Dailami Tjetjep Rohendi Rohidi, Tjetjep Rohendi Totok Sumaryanto Florentinus, Totok Sumaryanto Totok Sumaryanto, Totok Triani Widyaningrum Udi Utomo Umi Nur Sholikhah Utomo, Kasmidjo Budi W. Asmara W.Y. Rahman Wadiyo Wadiyo, Wadiyo Wardani, Anestia Widya Widaningtyas H, Adoniati Meyria Wijaya, Dhimayu Ichtiara Wijaya, Mas Benny Wilis Ari Ari Setyati1,2, Wilis Ari Wilis Ari Setyati Windi Amelia, Windi Wira Rahman Wira Y. Rahman Wisnu Nurcahyo Wulandari, Tita Yana, Rahma Husna Yatman, Teguh Mulyanto Yosita, Riesa Yudha Trinoegraha Adiputra Yulaida, Ita Yulius Docang Casessar Yuniarti, Ivana Yustiningrum, Giantari Ega