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Penapisan Bakteri Endofit Akar Kedelai Secara in Planta untuk Mengendalikan Penyakit Pustul Bakteri

Jurnal Fitopatologi Indonesia Vol 8, No 4 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Endophytic bacteria reside within plant hosts without causing disease symptoms. Bacterial endophytes promote plant growth and yield, suppress pathogens, may help to remove contaminants, solubilize phosphate, or contribute assimilable nitrogen to plants. The aim of this study were to obtain indigenous endophytic bacterial isolates, which have the ability to control bacterial pustule cause by Xanthomonas axonopodis pv. glycines and to increase growth and yield of soybean. The bacterial endophyte isolates were introduced as seed treatment (108 cfu/ml) and soil drench on 3 weeks old soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated on one month old of soybean by pricking leaf methodes. The effect of bacterial endophyte on disease incidence, disease severity, plant growth and yield of soybean were evaluated. We have found two selected bacterial endophyte isolates from soybean’s rhizosphere (St4E1.1 and St1E1.1.), with the ability to control bacterial pustule and to increase growth and yield of soybean.Key words: bacterial endophyte, bacterial pustule, in planta technique, soybean

Identifikasi Rizobakteria Asal Tanaman Cabai Berdasarkan Sekuen Gen 16S rRNA

Jurnal Fitopatologi Indonesia Vol 8, No 3 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Rhizobacteria was reported to have a potential role as inducer of systemic resistance in several host plants. Five isolates of rhizobacteria (RbTD1-3, RbTD1-8, RbPdGN-3, RbAg1-5, RbLPK1-9) were isolated from rhizosphere of chillipepper plants in West Sumatera. Identification of rhizobacteria was conducted by polymerase chain reaction using primers 27F and 1525R which amplified 16S rRNA region. DNA fragment of 1.5 kb was succesfully amplified from 4 isolates (RbLPK1-9, RbPdGN-3, RbTD1-3 and RbTD1-8) and subjected to direct sequencing. Phylogenetic analysis based-on 16S rRNA sequences showed that isolate RbLPK1-9 has a close relationship with Pseudomonas fluorescen Biotipe G (92%), whereas isolates RbTD1-3, RbTD1-8 and RbPdGN-3 have a close relationship with Bacillus subtilis 10407 (57%).Key words: polymerase chain reaction, rhizobacteria, 16S rRNA

Detection and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Pepper (Capsicum annuum) in West Sumatra, Indonesia

Microbiology Indonesia Vol 3, No 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

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Abstract

Yellow leaf curl disease of pepper has become an emerging important disease in West Sumatra since early 2000. Several attempts have been made, including disease survey and detection, in order to identify the causal agent of the disease. Pepper (Capsicum annuum) plants showing yellow leaf curl from West Sumatra were analyzed for presence of Begomovirus employing Polymerase Chain Reaction (PCR) with degenerate primers pAL1v 1978 and pARc 715. A DNA fragment of 1.6 kb was successfully amplified and subjected to direct sequencing. A stem loop region was found in the nucleotide sequence obtained, which contains the conserved nucleotide signature sequence TAATATTAC present in begomoviruses. Based on the stem loop region comparisons and phylogenetic analysis, the virus isolates from West Sumatra showed the closest relationship to Pepper yellow leaf curl Indonesia virus (PYLVIV) and Tomato yellow leaf curl Indonesia virus (TYLCIV). The sequence was different from other Asia Begomoviruses reported earlier. These isolates were divided into three groups which were tentatively called Pepper yellow leaf curl Indonesia virus-West Sumatra-[group 1], -[group 2] and -[group 3] {PYLCIV-WS-[group1], -[group2], and -[group3]}.

Penyakit Kerupuk Tembakau di Sumatera Barat

Jurnal Fitopatologi Indonesia Vol 10, No 6 (2014)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Tobacco leaf curl disease (“kerupuk”) is a new disease on tobacco in West Sumatra. This disease has spread throughout the tobacco planting area in District Limapuluh Kota with intensity of 2.5–26.3%. DNA fragment of 1600 bp was successfully amplified using polymerase chain reaction method with primer pairs for Begomovirus PAL1v 1978/PAR1c 715 from tobacco leaves showing symptoms. This result indicated Begomovirus infection. Sequence analysis based on NCBI BLAST program showed that Begomovirus infecting tobacco in West Sumatera has the highest homology of 89% and 91–97% with Pepper yellow leaf curl Indonesia Virus infecting tomato and chili pepper in West Sumatera, respectively. This is considered as the first report of Begomovirus infection on tobacco in West Sumatra.

SKRINING RHIZOBAKTERIA PENGHASIL INDOLE ANCETID ACID PENGINDUKSI PERTUMBUHAN DARI RHIZOSFIR CABAI (Capsicum annuum L.)

Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

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Abstract

Rhizobacteria was a colonialist bacterial at rooting area that had capacity to produce IAA and enhance plant growth. Rhizobacteria indigenus chili had ability to produce IAA and induces various of plant growth. IAA production was not always linked with the ability to enhance plant growth. Obtained there were 11 isolates with various of morphological characters and had ability as RMSD (Plant Growt Promoting Rhizobacteria) with an effective increase in growth from 57.88 to 82.80%.Key words: Rhizobacteria, IAA, Growth, Chili Rhizosfer

Identifikasi Molekuler Begomovirus Penyebab Penyakit Kuning Keriting pada Tanaman Cabai (Capsicum annum L.) di Sumatera Barat

Jurnal Natur Indonesia Vol 13, No 01 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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Abstract

Pepper plants showing Begomovirus-like symtoms, consisting of yellowing, leaf curling, and distortion, werecollected from fields located in the Padang, West Sumatra. The aim of this research is to identification ofbegomovirus associated with yellow leaf curl diseases on pepper. Total DNA was extracted from infected leaftissue according to Doyle and Doyle (1999) with slight modification. Polymerase chain reaction (PCR) was used toamplify the coat protein region of the virus using universal degenerate primers pAV494 and pAC1048. The PCRamplified DNA product (approx. 560 bp) was sequenced. The nucleotide and amino acid sequences and BLASTsearch revealed highest homology with pepper yellow leaf curl Indonesia virus isolated pepper, tomato andAgeratum conyzoides from Java, but differences from those of tomato yellow leaf curl virus. The isolate was thententatively called pepper yellow leaf curl Indonesia virus-Padang (PYLCIV-Pdg).

DISEMINASI TEKNOLOGI PUPUK KANDANG SAPI PLUS RIZOBAKTERI PADA KELOMPOK TANI KAKAO DI KABUPATEN LIMAPULUH KOTA

Jurnal Hilirisasi IPTEKS Vol 1 No 4a (2018): Desember 2018
Publisher : LPPM Universitas Andalas

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Abstract

Kegiatan diseminasi teknologi pupuk kandang sapi plus rizobakteri pada tanaman kakao dilakukan di Kecamatan Guguak dan Akabiluru Kabupaten Lima Puluh Kota yang dimulai dari bulan Maret sampai dengan November 2018. Kegiatan ini bertujuan untuk mengenalkan teknologi formulasi pupuk kandang plus rizobakteri pada kelompok tani kakao. Inovasi, buah lobek, maju sejahtera dan aroma menggunakan metode penyuluhan. Pentingny kegiatan ini dilakukan disebabkan oleh tingginya kejadian dan keparahan penyakit VSD di Kecamatan Guguak dan Akabiluru Kabuparten Limapuluh Kota yang mencapai 86,60%. Untuk itu, diperlukan teknologi pengendalian yang mudah dibuat, murah dan bahan baku yang mudah diperoleh disekitar petani. Penggunaan air kelapa merupakan salah satu perbanyakan bakteri Serratia maresescens dan Pseudomonas fluorescens yang nanti akan diformulasi dengan pupuk kandang. Kegiatan ini meliputi: pemantauan tingkat serangan OPT dilahan kelompok tani kakao, kemudian dilanjutkan dengan penyuluhan, pelatihan, dan evaluasi hasil kegiatan.  Dari hasil kegiatan bahwa kegiatan ini mampu meningkatkan pengetahuan petani terhadap penggunaan biofertilizer yang berasal dari bakteri yang diformulasikan dengan air kelapa dan pupuk kandang, sehingga penggunaan formulasi ini mampu mengurangi biaya penggunaan pupuk kimia yang memiliki harga yang mahal.