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Analisis Marka RAPD yang Te rpaut dengan To leransi terhadap Naungan pada Kedelai Wirnas, Desta; Sopandie, Didy; Trikoesoemaningtyas, ,; Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of the research was to identify RAPD markers linked to QTL related to agronomic traits of soybean under low-light intensity condition.  The genetic material used in the QTL analysis based on RAPD Markers were  Ceneng, Godek, and  F6 RILs derived from hybridization between  Ceneng (tolerant parent) and Godek (sensitve parent). The results of molecular analysis showed that 9 primers were polymorphic and linked to the tolerant parent.  Primers produce 14 RAPD markers which were polymorphic and linked to the tolerant parent. The markers were distributed into a linkage group that containing seven markers. RAPD Markers (OPE15-800, OPM20-800) were linked to two QTL controlling number of productive node and seed weight, respectively.  The marker linked to the tolerant parent could be used as a marker assisted selection for high-yielding soybean lines under low-light intensity.    
Keragaman Karakter Komponen Hasil dan Hasil pada Genotipe Kedelai Hitam Wirnas, Desta; Trikoesoemaningtyas, ,; Sutjahjo, Surjono Hadi; Sopandie, Didy; Rohaeni, Wage Ratna; Marwiyah, Siti; Sumiati, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 3 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

The objective of this research was to determine the trait variance in black soybean lines in terms of yield and yield components. The materials used were F8 lines generated from hybridization of two local varieties, i.e. black seeded Ceneng and greenish yellow seeded Godek, and then selected under single seed descent method, except SC-39-1, SC-62-2 and GC-74-7 that were generated through bulk selection method. The results showed that the traits between plant genotypes were significantly different, except number of productive node and number of unfilled pod. Total variance was contributed more by number of filled pod, seed weight per plant and 100 seed weight. Seed weight per plant and 100 seed weight had high heritability with genetic variance and broad-sense heritability which were significantly different from zero. These characters are important for further selection to improve yields of black soybean lines.Keywords: black soybean lines, broad sense heritability, total variance
Karakter Morfo-Fisiologi Daun, Penciri Adaptasi Kedelai terhadap Intensitas Cahaya Rendah Kisman, ,; Khumaida, Nurul; Trikoesoemaningtyas, ,; Sobir, ,; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The main objective of this study was to identify the leaf morpho-physiological characters as markers for adaptation of soybean to low light intensity. The field experiment was conducted using split plot design with 3 replications. First factor was soybean genotype consisted of shade tolerant genotypes (Ceneng, Pangrango) and sensitive genotypes (Godek, Slamet). Second factor was low light intensity consisted of: L0 = control (under full sun light), L1 = 5 days under 50% shading (exposed after 21 days after planting/DAP), L2 = 5 days under dark condition (exposed after 21 DAP), L3 = 3 days 50% shading + 5 days sun light (exposed after 18 DAP), dan L4 = 3 days 50% shading + 3 days sun light + 5 day dark conditions (exposed after 15 DAP). The 50% shading was made artificially using black plastic paranet with 50% light transmitted. Results of this study showed that under the conditions of low light intensity, leaf morpho-physiological characters of soybean that could be used as markers for adaptation were leaf area, specific leaf weight, and chlorophyll content. Leaves of shade tolerant genotypes were wider and thinner and also contained higher chlorophyll especially for chlorophyll b than that of shade sensitive genotypes under low light intensity.   Key words :  Low light intensity, tolerant genotype, leaf area, chlorophyll, specific leaf weight
Evaluasi Beberapa Kultivar Padi Gogo Asal Kalimantan Timur untuk Ketahanan terhadap Aluminium Menggunakan Metode Kultur Hara Rusdiansyah, ,; Rohaeni, Neni; Trikoesoemaningtyas, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 29, No 3 (2001): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

Twenty four upland rice cultivars from East Kalimantan and control cultivar were evaluated for tolerance to aluminium using nutrient culture with two treatments of 0 ppm and 45 ppm AI. Result showed that eight cultivars (Pimping, Ketan Hitam, Mayas, Padi Bulu, Popot, Siam Kuning, Ketan Merah, Serai) have PAR > 0.5 and were categorized as tolerant cultivar, the other 16 cultivars were categorized as suceptable. Three tolerant cultivars: Pimping, Ketan Merah and Mayas. even have PAR higher than a control, Krowal.   Key words: Rice, AI, PAR, Nutrient
Evaluasi Daya Pemulih Kesuburan Padi Lokal dari Kelompok Tropical Japonica Hairmansis, Aris; Aswidinnoor, Hajrial; Trikoesoemaningtyas, ,; Suwarno, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 33, No 3 (2005): Jurnal Agronomi Iindonesia
Publisher : Bogor Agricultural University

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Abstract

Twenty seven land races of tropical japonica rice were test-crossed with a ´WA´ type cytoplasmic male sterility (CMS) line IR58025A to evaluate their fertility restoration ability.  Based on pollen fertility and spikelet fertility of their F1 hybrids, genotypes are classified into maintainer or restorer.  The result showed frequency of maintainers among genotypes was higher than restorers.  Four genotypes, i.e., Ase Lapan, Ase Mandi, Hawara Bunar and Lampung Kuning were designated as maintainers.  These maintainers possess a number of desirable traits such as pest resistance and abiotic stresses tolerance; thus they will be useful for improving parental lines of hybrid rice.     Key words: Land race, test cross, fertility restoration
Pola Pewarisan Adaptasi Kedelai (Glycine max L. Merrill) terhadap Cekaman Naungan Berdasarkan Karakter Morfo-Fisiologi Daun Kisman, ,; Trikoesoemaningtyas, ,; Sobir, ,; Khumaida, Nurul; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this study was to analyze the inheritance pattern of soybean adaptation to shade stress based on leaf morpho-physiological characters. Genetic materials used in this study consisted of 22 plants of low irradiance (LI)-tolerant genotype (Ceneng) and 22 plants of LI-sensitive genotype (Godek); 21 plants of F1 (Ceneng x Godek); and 114 plants of F2 populations (derived from F1). These populations were planted under shading of paranet 50%. The population was arranged in a Randomized Complete Block Design with two replicates. Analysis of inheritance of soybean adaptation involved estimation of heritability (broad sense) and gene action. Results of this study showed that: adaptation of soybean to low light stress based on characters of yield per plant was highly heritable (68% of broad sense) with partial dominant mode of action. Characters of leaf area and specific leaf weight were highly (68% of broad sense) and moderately (48% of broad sense) heritable, respectively, with additive mode of action. Soybean adaptation based on leaf physiological characters (chlorophyll contents) was highly heritable (70% - 86% of broad sense) in epistatic mode of action.   Key words:  Soybean, leaf morpho-physiological character, heritability, gene action
Penyebab Kehampaan Gabah pada Persilangan Padi antar Subspesies Hermanasari, Rini; Aswidinnoor, Hajrial; Trikoesoemaningtyas, ,; Suwarno, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 2 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The  objective  of  the  research  was  to  study  the  factors  effecting  unfilled  grains  in  intersubspecific  hybridization.  The  research  was  conducted  at  Muara, Bogor  from  April  2005  to  February  2006.  The  materials  used  in the research were  ten  F1 hybrid  combinations.  The evaluation was done by crossing the hybrids to Ciherang and it was resiprocal. A randomized block design with three replications were arranged in greenhouse. The results showed that five cross combinations produced higher unfilled grains and lower pollen fertility due to defective male gamets. The cross combinations that is Akitakomaci/IR64, Akitakomaci/Wayrarem, Akitakomaci/Salumpikit, Akitakomaci/Ketupat, and Akitakomaci/Kewal. Another five cross combinations produced normal pollen fertility but high unfilled grains. The high unfilled grains of four cross combinations was due to defective female gamets. The cross combination that is IR64/Sirendah Pulen, IR64/Lampung Putih, IR64/Lampung Kuning, and IR64/Mesir.  The other cross combination was due to defective male and female gamets  namely IR64/Brentel.   Key words:  Unfilled grain, F1 hybrids, male gamets, female gamets
Pewarisan Sifat Toleransi Padi Sawah (Oryza sativa L) Terhadap Cekaman Suhu Rendah Limbongan, Yusuf L.; Aswidinnoor, Hajrial; Purwoko, Bambang S.; Trikoesoemaningtyas, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 2 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

One of the  definite successful of rice breeding programs for increasing yield and tolerance to environmental stress condition is selection method.  Selection method will be effectively if it is supported by a perfect knowledge of genetics character inheritance.  The objectives of this study is to investigate the information of inheritance pattern adapted of rice towards low temperature stress condition.  The experiment was conducted in both Sesean rural area, Toraja regency, South Sulawesi on 1500 m above sea levels, an mean temperature 15 oC, type of soil is Podzolic and at Bogor Agriculture Institute.  This experiment is done in January 2006 until July 2007.  The result showed that tolerance parental grew well and higher yield than sensitive parental.  There were no significant differences between F1 and F1 reciprocal for all characters.  Tolerance parental is more superior than sensitive parental in all characters have been tested.  Mean of  F1 generation is in between tolerance parental and sensitive parental.  BCP1 has spheres wider than parentals and F1 generation, but BCP2 smaller than parentals and F1 generation.  F2 generation is spheres wider than the average of both parentals.  The m [d] [h] genetic model is the most suitable for all characters.  Except, date of flowering and weight of filled rice where appropriate models is m [d] [h] [l].  Broad sense and narrow sense heritability is range between low to high.  Based on the degree of dominant, additive effect is higher than dominant effect. The negative F value reached on date of flowering and fur lenght; and others characters, F value is positive.    Key words: Low temperaure stress tolerance, backcross, reciprocal,  broad sense heritability, narrow sense heritability
Adaptasi Tanaman Kedelai Terhadap Intensitas Cahaya Rendah : Karakter Daun untuk Efisiensi Penangkapan Cahaya Muhuria, La; Tyas, Kartika Ning; Khumaida, Nurul; Trikoesoemaningtyas, ,; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 34, No 3 (2006): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

This research was conducted to identify some leaf characters related to light capture efficiency. It used split plot design with sub plot nested at the main plot. The main plot was light intensity consisted of two levels: I100 = light intensity 100% and I50 = light intensity 50%, while the sub plot was soybean genotype consisted of G1 = Pangrango, G2 = Ceneng, G3 = Godek, and G4 = Slamet.  Leaf characters measured were : leaf total area, specific leaf area, trifoliate leaf area, leaf hairs (trichome) density, leaf thickness, length of  palisade, chlorophylls content (chlorophyll a, b, and ratio a/b), and the greenness intensity.  The result of research indicated that Ceneng has lower total leaf area, higher specific and trifoliate leaf area, less leaf hair density, thinner leaf, shorter palisade, higher greenness intensity, higher chlorophyll content (a and b), and also lower ratio chlorophyll a/b.   Key words:  Light capture efficiency, light intensity, tolerant genotype, sensitive genotype, soybean
Konstribusi Akumulasi Silikat, Nitrogen dan Aluminium terhadap Ketenggangan Aluminium dan Ketahanan terhadap Penyakit Blas pada Padi Gogo Bakhtiar, ,; Purwoko, Bambang Sapta; Trikoesoemaningtyas, ,; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 3 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Aluminum (Al) toxicity and blast disease are the most important yield-limiting factors for upland rice production in acid soils. The objective of this experiment was to examine the contribution of accumulation of Silicate (Si), Nitrogen (N) and Al in plant tissue on Al tolerance and blast disease resistance in upland rice. The experiment was arranged in a split-split plot design with 2 replications. Main plots were randomly assigned to blast treatment (control and inoculation to blast fungi). Subplots were assigned to control box (lime 1.5 AlEC) and another acid soils (no lime) box and sub-subplots were assigned to the tested genotypes. The result of the experiment showed that leaf blast disease resistance in rice cannot be solely explained by Si or N content in shoot tissue. The resistant to leaf blast disease might be attributed by high ratio Si/N weight in shoot. Al tolerance was ascribed by low reduction in root growth, high shoot dry weight, high Si content in shoot, and as well as high of  Si/Al ratio in root.   Key words:  Al-tolerance, blast disease, upland rice, Si/Al ratio