Risa Tiuria
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Bogor Agricultural University (IPB)

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PREVALENCE OF TREMATODES IN JAVAN RHINOCROS AND BANTENG IN UJUNG KULON NATIONAL PARK

Jurnal Veteriner Vol 9, No 2 (2008)
Publisher : Jurnal Veteriner

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Abstract

Javan rhinoceros (Rhinoceros sondaicus) and Javan bull (Bos javanicus) in National Park Ujung Kulon are protected wild animals. A study to examine the presence of trematode eggs in their fecal samples was carried out. The fecal samples were collected based on the transec route in National Park Ujung Kulon. The presence of eggs in fecal samples was examined by filtration method. Trematode eggs were found in 56% of Javan rhinoceros fecal samples and 73,91% of Javan bull fecal samples.. Base on the size of the eggs it was determined that Javan bulls in National Park of Ujung Kulon were infected by Fasciola spp (17,39%) and Paramphistomum spp (56,52%). Javan rhinoceros in National Park of Ujung Kulon was infected with Fasciola spp (44%) and Schistosoma spp (12%). This study clearly showed that tramatode infection is commom among wild ruminants in Ujung Kulon National Park and attention should be taken to improve the health status of the animals especially Javanese rhinoceros and Javanese bull

An estimate of meat production loss in native chicken in Bogor and its surrounding districts due to gasterointestinal helminthiasis

Hemera Zoa Vol 74, No 3 (1991): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Among 78 native chicken from Bogor and its surrounding districts, only  4 (5.1%) with mean dressing weights of 1250 ± 29 g were free from parasiric worms. The other 74 chicken found infected with nematodes [mean nematode numbers (mn) = 24]. Cestodes (mc = 45) and trematodes (mt = 7). Single parasite class infections of nematode (mn = 18) and of trematode (mt = 96) were each one sample (1.3% with mws 1250 and 1180 g, respectively); single parasite class infections of cestode (mc = 47) were 15 (19.2% wit mw = 1145 g). Total single parasite class infections were 17 (21.8%). Mixed nermatode (mn = 29) + castode (mc = 50) were 41 (52,6%; mw = 1056 g), nematode (mn = 4) + trematode (mt = 28) were 2 (2.6% mw = 1225 g) and cestode (mc = 44) + trematode (mt = 6 - were 2 (2,6%; ws = 975 g). Total two parasite class infections were 45 (57.7%). Mixed infections of nematode (mn - 45) + cestode (mc = 44) + trematode (mt = 27) were 12 (15,4%; mw = 1034 g).

Taksiran kerugian produksi daging akibat infeksi cacing saluran pencernaan pada ternak domba

Hemera Zoa Vol 74, No 2 (1991): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Sebanyak 100 ekor domba jantan dan betina yang berasal dari Kotamadya dan Kabupaten Bogor dicatat berat karkasnya pada waktu penyembelihan di jagal dan contoh tinjanya diperiksa secara kuantitatif terhadap telur cacing saluran pecernaan. Terdapat korelasi negatif yang nyata (r= 0,4126; dk=3 : 76; P < 0,005) dengan indeks determinasi (r2) 0,1702, antara jumlah ttgt didalam tinja dengan cerat karkas domba. Dari sampel dengan rata-rata berat karkas 16,3 kg terdapat 80% dengan rata-rata berat karkas 15,4 kg yangmengandung telur cacing nematode, cestoda dan/atau trematoda. Hanya 20% yang negative dengan rata-rata berat karkas 19.8 kg. Dari yang positif, 27% menderita infeksi tunggal nematode, 7% cestoda, 8% trematoda, 9% infeksi campuran nematode dan cestoda, 7% nematode dan trematoda, 9% cestoda dan trematoda serta 13% infekasi campuran nematode, cestoda plus trematoda. Dari infeksi tunggal yang berjumlah 38% dan infeksi campuran sebanyak 42% terdapat 56% infeksi nematode, 38% cestoda dan 37% trematoda. Infeksi tunggal nematode mengakibatkan penurunan berat karkas sebesar 21, 72%, cestoda 9,60% dan trematoda 7,07% disbanding dengan berat karkas kelompok negatif. Infekski campuran nematode dan cestoda mengakibatkan penurunan produksi daging yang paling banyak (41,92%), disusul oleh infeksi campuran nematode, cestoda plus trematoda (34,34%). Domba jantan mengalami infeksi yang lebih berat dengan presentase penurunan berat karkas yang lebih besar disbanding dengan domba betina. Helminthiasis, ditambah interaksi dengan factor-faktor lain, mengakibatkan kerugian produksi daging dari ternak domba yang ditaksir antara 17,75 – 24,77% atau 3,2 – 4,4 juta kg atau Rp. 7,68 – 10,56 milyar atau US$ 4,8 – 6.6 juta pertahun.

Populasi Ascaridia galli Dalam Usus Halus Ayam Yang Diberikan Kombinasi Ekskretori/Sekretori L3 dan Imunoglobulin Yolk

Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

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Abstract

Ascaridia galli populations in intestine of chickens treated with combination of excretory/secretory L3 and immunoglobulin yolkABSTRACT. The purpose of the present study was to determine the presence of worm populations in intestine of chickens vaccinated and combined with egg yolk to experimental Ascaridia galli infection. Amount of 18 head chickens were devided into six groups (A – F). Group A, the chickens were not vaccinated. Group B, the chickens were vaccinated with excretory/secretory of A. galli L3. Group C, the chickens were vaccinated with excretory/secretory of A. galli L3, challenged with dose 1000 L2, and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group D, the chickens were vaccinated with excretory/secretory of A. galli L3 and challenged with dose 1000 L2. Group E, the chickens were challenged with dose 1000 L2 and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group E, the chickens were challenged with dose 1000 L2. Intestinal worm burdens of infected groups were recorded. The result showed that excretory/secretory of A. galli L3 combined with egg yolk decreased significantly A. galli survival in intestine of laying hens. Vaccinations were positively correlated with worm burden at 12 weeks after chalanged. The results suggest that A. galli L3 excretory/secretory product contain potential antigen and that antibody-mediated mechanisms contribute to immune protection.

KONSENTRASI PROTEIN DAN PENENTUAN BERAT MOLEKUL EKSKRETORI/SEKRETORI L3 Ascaridia galli

Jurnal Kedokteran Hewan Vol 3, No 1 (2009): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein  ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100  ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 5–10  L3 dikultur secara in vitro  dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 µg ml-1 streptomisin, 5 µg ml-1 gentamisin dan 0,25 µg ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian  menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.

Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli

Jurnal Agripet Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010
Publisher : Agricultural Faculty

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Abstract

Purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli L3 larvae stageABSTRACT. The main immunoglobulin fraction of poultry is called IgY, in order to distinguish it from the mammalian IgG. This article focus on purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli larvae to obtained purity IgY. Active vaccinations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 μg. The vaccinations were repeated three times with dose of each 60 μg with an interval of one week. The first vaccinations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by agar gel precipitation test (AGPT). The chicken’s eggs were collected from 49 day after vaccinations. IgY was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ekscretory/secretory IgY protein was determined by Bradford method (λ = 280 nm). The result showed that antibody in yolk was begun detect with AGPT at four weeks after vaccination. IgY concentration after purification was 0,875 ± 0.251 mg/ml. This study has shown that the product released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing antibody through yolk as biologic manufacturer could be a good choice.

L3 Populations in Laying Hens Infected with 6,000 L2 of Ascaridia galli

Jurnal Kedokteran Hewan Vol 1, No 2 (2007): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae

Struktur Komunitas Cacing Parasitik pada Ikan Kembung (Rastrelliger Spp.) di Perairan Teluk Banten dan Pelabuhan Ratu

Jurnal Ilmu Pertanian Indonesia Vol 19, No 1 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The short mackerel is the most commercially important small pelagic fish in Indonesia. Parasitism plays a central role in fish biology. Parasitism is a ubiquitous phenomenon in the marine environment and it is probable that all marine fishes are infected with parasites. Helminth parasitic of Rastrelliger spp. are Lechitocladium angustonum (Digenea: Hemiuridae), Lecitochirium sp. (Digenea: Hemiuridae), Prodistomum orientalis (Digenea: Lepocreadiidae) and Anisakis typica (Nematodes: Anisakidae), with 90.12% of prevalence. They are not significant different of helminth parasitic abundance from R. kanagurta and R. brachysoma, but significant in helminth species richness. This different of helminth species richness was because of L. angustonum dominances. The different location wasn’t have significant different of helminth parasitic abundance because Indonesian in the tropical zone. Anisakis species in Java sea have a same genetic with Anisakis typical and not zoonotic parasite kategories. The fish digestion was a microhabitat for helminth parasitik because they have much food stuff. The fish growth have significant different in helminth parasitic abundance and intencity. This situation was because the fish immunity development and the food habit of fish. Keywords: Banten bay, community structure, helminth parasites, Pelabuhan Ratu bay, Rastrelliger spp.

The effect of excretory/secretory product released by L3 of A. galli on villous compact in intestine of laying hens

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The nematode, Ascaridia galli, caused pathological changes in the gut, while the larval development takes place in the intestine epithelium. The aim of this research was to examine the effect of excretory/secretory product released by L3 of A. galli to prevent intestine destructions based on villous compact in duodenum, jejunum, and ileum of laying hens. Excretory/secretory was prepared from metabolic of L3 released in culture médium in flasks containingrosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Excretory/secretory product was concentrated with vivaspin 30.000 MWCO. The chickens vaccinated with 80 >g protein excretory/secretory in crudemixed with Fruend Adjuvant Complete and repeated three times with dose of each 60 >g mixed with Freund Adjuvant Incomplete with an interval of one week intra muscularly. The chickens were challenged with 1000 infective larvae A. galli one week later. After two weeks the chickens were operated. Villous compact in duodenum, jejunum, and ileum of laying hens were analyzed. The result showed that immunization was able to sustained significantly intestinevillus compact, particularly in duodenum. This research concluded that the excretory/secretory product could protect villus in duodenum against parasitic diseases caused by A. galli.

Protozoa Parasitik pada Ikan Sidat (Anguilla spp.) Asal Danau Lindu, Sulawesi Tengah

Jurnal Ilmu Pertanian Indonesia Vol 20, No 2 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Infection protozoan parasitic on eels (Anguilla spp.) from Lindu Lake was affected weight eels and health eels. The protozoan parasitic examinations were collection of eels, inspection eels, observation, measurement, identification of parasites, and staining protozoan used giemsa stained, trematode used semichons’s acetocarmine stained and nematode used clove oil and KOH. The diversity of parasitic protozoan found such as: Myxidium sp., Myxobolus sp., Chilodonella sp., Ceratomyxa sp., Balantidium sp., Henneguya sp., and Glugea sp. The highest prevalence Myxidium sp. in eels was 77% and no protozoan dominated between other parasites. Based on chi-square test, protozoan parasitic had a different preference to organs of eels.