Hartini Tiono
Bagian Histologi, Fakultas Kedokteran, Universitas Kristen Maranatha

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Pengaruh Pasta Tomat (Solanum lycopersicum) terhadap Diameter Tubulus Seminiferus Mencit (Mus musculus) Galur DDY yang Terpajan Asap Rokok Berfilter Sugeng, Sri Utami; Tiono, Hartini; Anandaputri, Vikie N.
Jurnal Kedokteran Maranatha Vol 10, No 1 (2010)
Publisher : Universitas Kristen Maranatha

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Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh pasta tomat (Solanum lycopersicum) terhadap diameter tubulus seminiferus mencit jantan galur DDY yang terpajan asap rokok berfilter. Penelitian ini menggunakan metode prospektif eksperimental laboratorium sungguhan dengan Rancangan Acak Lengkap (RAL) yang bersifat komparatif. Dua puluh lima ekor mencit jantan galur DDY dibagi secara acak dalam 5 kelompok perlakuan (n=5). Pada hari pertama kelompok kontrol akuades dan kontrol asap rokok berfilter diberi akuades, sedangkan tiga kelompok pasta tomat diberi pasta tomat yang dilarutkan dalam akuades dengan dosis 0,16 g, 0,32 g, dan 0,48 g secara per oral. Pada hari ke-7 kelompok kontrol asap rokok berfilter dan 3 kelompok pasta tomat dipajankan pada asap rokok berfilter. Pada hari ke-14 semua mencit dikorbankan dan dibuat sediaan histopatologis tubuli seminiferi. Data dianalisis menggunakan uji ANAVA satu arah dengan α=0,05 yang dilanjutkan dengan uji beda rata-rata Tukey HSD. Hasil penelitian menunjukkan, terdapat perbedaan yang signifikan antara rata-rata diameter tubulus seminiferus kelompok pasta tomat dosis II (p=0,014) dan III (p=0,032) dengan kelompok kontrol asap rokok berfilter. Simpulan penelitian adalah pasta tomat meningkatkan diameter tubulus seminiferus pada mencit yang terpajan asap rokok berfilter.Kata kunci: pasta tomat, likopen, asap rokok berfilter
Effect of Garlic Tuber Juice (Allium sativum L.) in Wound Healing Shorten the Duration of Swiss Webster Mice Dewi Timotius, Ivana Cyntia; Puradisastra, Sugiarto; Tiono, Hartini
Jurnal Medika Planta Vol 2, No 1 (2012)
Publisher : Jurnal Medika Planta

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Introduction:Wound is a very common thing that can be happened to all people around the world, starts from children to adults. We can treat wounds by using modern medicine and herbs medicine. One of the most commonly used herbs in the community is garlic (Allium sativum L.).Objectives: The purpose of this research is to investigate the effect juice of garlic (Allium sativum L.) in accelerating the healing wound process.Methods: In this study, we use a comparative true experimental design method with 25 mice with average be a five groups of mice were made cut along 8 mm and treated with juice of garlic 2.5%, juice of garlic 5%, juice of garlic 10%, distilled water, 1% chloramphenicol. We observed the healing wound process until the both wound edges interlocked. Data analysis is using one-way ANOVA which is continued by Tukey HSD, α = 0,05. Results: The mean wound healing time in days with juice of garlic 2.5% (5), juice of garlic 5% (5), juice of garlic10% (5.6) show a highly significant difference compared with the control negative group with p=0,000, p=0,000, p=0,000.Conclusion: Juice of garlic (Allium sativum L.) has effect in accelerating healing wound process.
The Comparison Effect of Aloe vera L., Psidium guajava Linn, Curcuma domestica Val to Colitis Ulcerative Mice Models Histopatology Kurniawati, Dewi; Jasaputra, Diana Krisanti; Ratnawati, Hana; Tiono, Hartini; Sujatno, Muchtan; Dewi, Aprilin Krista; Mayestica S., Putu; Arifin, Samuel
Jurnal Medika Planta Vol 1, No 2 (2010)
Publisher : Jurnal Medika Planta

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Introduction: Ulcerative colitis (UC) is an inflammatory disease limited to the mucosal and submucosal layers of the colon. UC treatments was aimed to decrease inflammation and oxidation process on colon. Nowadays people start using hermal medicine to attentuate inflammation and oxidation process in ulcerative colitis, e.g. Aloe vera, Psidium guajava Linn, Curcuma domestica Val.Objective: to compare the effect of Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract on histopatology of ulcerative colitis.Methods: experimental comparative research with completely randomized design. Mice were divided on 5 groups (n=6) which were given Aloe vera ethanol extract 700 mg/kgBB and DSS 2,5%; Psidium guajava Linn ethanol extract 1.591,2 mg/kgBB and DSS 2,5%; Curcuma domestica Val ethanol extract 180.816 mg/kgBB and DSS 2,5%; aquadest and DSS 2,5% (positive control); aquadest (negative control). Data were analyze with ANOVA followed by Tukey HSD test with a=0.05.Results: there were a decrement percentage of crypt loss on Aloe vera ethanol extract group (10.5 +- 4,64681%), Psidium guajava Linn ethanol extract group (11 +- 2,09762%), Curcuma domestica Val ethanol extract group (9 +- 3,09839%) compared with positive control group (26.8 +- 1,72240%)(p<0.05). Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract had same effectivity (p>0.05).Conclusion: Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract had same effectivity on decreasing percentage of crypt loss on colitis ulcerative histopatology of mice induced by DSS 2.5%.
The Preventive Effect of Fig Leaves’s (Ficus carica L.) towards Colon Histopathological Feature and IL-6 Serum Level on Ulcerative Colitis Induced Mice Tiono, Hartini
Journal of Medicine and Health Vol 1, No 4 (2016)
Publisher : Maranatha Christian University

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Abstract

Ulcerative colitis is a chronic inflammatory disease mainly affects sigmoid colon and rectum. The inflammation process will activate NF-κB and leads to proinflamatory cytokine release such as Interleukin-6 (IL-6). Fig leaves contain a high level of flavonoid which can prevent NF-κB activation, and further inhibits IL-6 secretion. This research aims to see the preventive effect of methanolic extract of fig leaves towards colon histopathological feature and IL-6 serum level on ulcerative colitis induced mice. Balb/C male mice were randomly assigned into 5 groups (n=5). The treatment groups were dextran suphate sodium (DSS) control group (group I), methanolic extract of Fig leaves dose 28 mg/ day control group (group II), and methanolic extract of Fig leaves dose 7 mg/ day (group III), 14 mg/ day (group IV), and 28 mg/ day (group V) for 14 days, which at the 8th till 14th day were given DSS to induce colitis. The results showed that both of colon mucosal damage and IL-6 serum level of group I were significantly different from other groups (p=0,029). In  conclusion, the methanolic extract of Fig leaves can improve colon mucous damage and decrease IL-6 serum level on ulcerative colitis-induced mice. Keywords: ulcerative colitis, fig leaves, colon mucousal damage, interleukin-6
The Preventive Effect of Fig Leaves’s (Ficus carica L.) towards Colon Histopathological Feature and IL-6 Serum Level on Ulcerative Colitis Induced Mice Tiono, Hartini
Journal of Medicine & Health Vol 1 No 4 (2016)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (733.331 KB) | DOI: 10.28932/jmh.v1i4.529

Abstract

Ulcerative colitis is a chronic inflammatory disease mainly affects sigmoid colon and rectum. The inflammation process will activate NF-κB and leads to proinflamatory cytokine release such as Interleukin-6 (IL-6). Fig leaves contain a high level of flavonoid which can prevent NF-κB activation, and further inhibits IL-6 secretion. This research aims to see the preventive effect of methanolic extract of fig leaves towards colon histopathological feature and IL-6 serum level on ulcerative colitis induced mice. Balb/C male mice were randomly assigned into 5 groups (n=5). The treatment groups were dextran suphate sodium (DSS) control group (group I), methanolic extract of Fig leaves dose 28 mg/ day control group (group II), and methanolic extract of Fig leaves dose 7 mg/ day (group III), 14 mg/ day (group IV), and 28 mg/ day (group V) for 14 days, which at the 8th till 14th day were given DSS to induce colitis. The results showed that both of colon mucosal damage and IL-6 serum level of group I were significantly different from other groups (p=0,029). In  conclusion, the methanolic extract of Fig leaves can improve colon mucous damage and decrease IL-6 serum level on ulcerative colitis-induced mice. Keywords: ulcerative colitis, fig leaves, colon mucousal damage, interleukin-6