B. Tiesnamurti
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Methane emission factors for enteric fermentation in beef cattle using IPCC Tier-2 method in Indonesia Widiawati, Yeni; Rofiq, M.N.; Tiesnamurti, B.
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 2 (2016): JUNE 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

Methane emission from enteric is a sub-category considered under the Agriculture sector greenhouse gas emissions by UNFCCC, thus Indonesia developed calculation on enteric CH4 EF for ruminant using Tier-2 method as country-specific emission factors (EF). Indonesia has huge amount of beef cattle population, which contributes significant amount to national enteric methane emission. The aim of this study was to estimate enteric methane EF for beef cattle in Indonesia using IPCC Tier-2 method.  The EF generated from this study is then used to estimate the methane emitted from beef cattle. Data on beef cattle population was obtained from BPS, data on energy content of feed, feed intake and digestibility were compiled from laboratory analysis and published paper. Equations were adopted and followed the instruction of IPCC 2006. Local cattle has different CH4 EF among each sub-category, which are  ranging from 18.18 to 55.89 Kg head-1 yr-1, with the average of 36.75  head-1 yr-1. Imported beef cattle has lower  CH4 EF (25.49 kg head-1 yr-1) than the average for local beef cattle. Overall, the national CH4 EF of beef cattle calculated by using IPCC Tier-2 method in Indonesia is 33.14 head-1 yr-1. The value is lower than default EF from IPCC for Asia country (47 kg head-1 yr-1). The conclusion is enteric CH4 EF for beef cattle in Indonesia calculated using Tier-2 method shows the real livestock system in Indonesia condition. Further research needed to be addressed are calculation of EFs for various breeds and feeding systems, since large variations of breeds and types of feed among provinces in Indonesia.
Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Batubara, Aron; Noor, R.R.; Farajallah, A.; Tiesnamurti, B.; Doloksaribu, M.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

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Abstract

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats
Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Batubara, Aron; Noor, R.R.; Farajallah, A.; Tiesnamurti, B.; Doloksaribu, M.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (136.805 KB)

Abstract

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats
Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Batubara, Aron; Noor, R.R.; Farajallah, A.; Tiesnamurti, B.; Doloksaribu, M.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Original Source | Check in Google Scholar | Full PDF (136.805 KB)

Abstract

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats