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The Selection of Xylanase-Producing Indegenous Bacteria Richana, Nur; Lestari, Puji; Thontowi, Ahmad; ., Rosmimik
Jurnal Mikrobiologi Indonesia Vol 5, No 2 (2000): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

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Abstract

The isolation and selection of xylanase producing bacteria have been done from soil and waste of starch Industry.Strains were cultivated in neutral and alkaline media with xilan as a substrate. Colonies which produced clearingzone were presumed as xylanolytic bacteria and chosen for further screening. Results show that 10 isolates ofbacteria In neutral condition and 25 bacteria in alkaline condition. There are three isolates in neutral condition ON.12, ON-13 and ON-33 have high enzyme activities at 19.93, 23.42 and 19.32 U/ml, and specific activities which were80.37, 71.85 and 114.36 U/mg protein respectively. On the other hand there are two isolates in alkaline condition TAIS and TA-fl15 which have high enzyme activities at 12.43 and 11.18 U/mI, and specific activities which were 36.72 and42.68 U/mg protein respectively.
Pertumbuhan Bakteri Laut Shewanella indica LBF-1-0076 dalam Naftalena dan Deteksi Gen Naftalena Dioksigenase - (The Growth of Marine Bacteria Shewanella indica LBF-1-0076 in Naphthalene and Naphthalene dioxygenase Gene Detection) Farini, Nuzul; Thontowi, Ahmad; Yetti, Elvi; Suryani, Suryani; Yopi, Yopi
Biopropal Industri Vol 8, No 1 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Crude oil exploitation which often occured offshore can cause water pollution in the sea since its contains naphthalene which is a hazardous compounds. This research used marine bacteria LBF-1-0076 that have ability in naphthalene degradation. This research aimed to study the parameter effect of naphthalene and cell concentration toward marine bacteria LBF-1-0076. This research also identified isolate LBF-1-0076 and detected the encode gene of naphthalene dioxygenase. Based on growth test result, the optimum naphthalene degradationby isolate LBF-1-0076 occured in 75 ppm naphthalene concentration with 15cell concentration. The result of 16S rDNA gene analysis showed that LBF-1-0076 was identified as Shewanella indica strain 0102 with identical value 99%. The result of naphthalene dioxygenase gene detection using Polymerase Chain Reaction (PCR) showed that the isolate contained naphthalene dioxygenase gene with size ±377 bp. Therefore, LBF-1-0076 potential as bioremediation agent to solve crude oil contamination in the sea.Keywords:   crude oil, marine bacteria, naphthalene, naphthalene dioxygenase, Shewanella indicaABSTRAKEksploitasi minyak bumi yang sering terjadi di laut mengakibatkan adanya pencemaran minyak di laut. Naftalena merupakan salah satu senyawa dominan berbahaya yang terkandung dalam minyak bumi dan dapat mengakibatkan pencemaran perairan. Penelitian ini menggunakan bakteri laut LBF-1-0076 yang memiliki kemampuan untuk mendegradasi naftalena. Tujuan dari penelitian ini adalah mempelajari pengaruh parameter konsentrasi naftalena dan konsentrasi sel terhadap bakteri laut pendegradasi naftalena LBF-1-0076. Penelitian ini juga bertujuan untuk mengidentifikasi isolat LBF-1-0076 dan mendeteksi gen pengkode naftalena dioksigenase. Berdasarkan hasil uji pertumbuhan, degradasi naftalena yang optimal oleh isolat LBF-1-0076 terjadi pada konsentrasi naftalena 75 ppm dengan konsentrasi sel 15. Hasil analisis gen 16S rDNA menunjukkan isolat LBF-1-0076 teridentifikasi sebagai Shewanella indica strain 0102 dengan nilai keidentikan 99%. Hasil deteksi gen naftalena dioksigenase dengan menggunakan Polymerase Chain Reaction (PCR) menunjukkan bahwa isolat tersebut mempunyai gen naftalena dioksigenase dengan ukuran ±377 bp. Oleh karena itu, isolat LBF-1-076 berpotensi sebagai agen bioremediasi untuk mengatasi masalah pencemaran minyak bumi di laut.Kata kunci: bakteri laut, minyak bumi, naftalena, naftalena dioksigenase, Shewanella indica
Alkane Degradation and Detection of Mono-xygenase Gene from Alcanivorax sp. from Jakarta Bay Thontowi, Ahmad; Yopi, Yopi
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.757 KB) | DOI: 10.1234/48

Abstract

Alkanes is a major component of crude oil that can be hydrolized by enzyme alkane monooxygenase from bacteria. Nine oil-degrading bacteria were analyzed their capability to degrade alkanes (pristane and paraffin). The result of growth test on paraffin and pristane were showed that 9 isolates could be devided into two groups. First group (BL09, BL31 and BL45) could degrade both paraffin and pristane, and second group (BL01, BL06, BL44, BL057, BL058 and BL071) preferred to degrade paraffin than pristane. Three isolates (BL09, BL31 and BL45) have activity to decrease paraffin and pristane until less 50% remain. Based on homology analysis of 16S rRNA gene sequences showed that isolates No. BL09, BL31 and BL45 were identified as Alcanivorax sp. and the partial sequences of the alkB gene from those three isolates are showing 66-68% of identity compare with some mono-oxygenase gen from database of genbank.Keywords: biodegradation, alkane, monooxygenase, cloning, alkB
Pertumbuhan Optimal Bakteri Laut Pseudomonas aeruginosa LBF-1-0132 dalam Senyawa Piren Safitriani, Safitriani; Thontowi, Ahmad; Yetti, Elvi; Suryani, Suryani; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.628 KB) | DOI: 10.14203/jbi.v13i1.3100

Abstract

ABSTRACTPyrene is a high molecular weight chemical compound belongs to polycyclic aromatic hydrocarbon (PAHs) group that are difficult to degrade by environment. Biodegradation techniques using indigenous marine bacteria are used to be as an effort to reduce pollutants that are carsinogenic. The objectives of this research are to screen of 18 marine bacteria isolates qualitatively by sublimation method and quantitatively by growth test and to optimize degradation activity of marine bacteria isolates by pyrene concentration and cell concentration. Identification by 16S rDNA and phylogenetic tree analysis were conducted to determine the molecular basis of bacterial identity. The result of sublimation showed that 15 isolates were positive result for pyrene degradation and classified to 3 groups. The first group consisted of 5 isolates that can produce clear zone, while the second group are 5 isolates with isolate color changes. The third group have both of activities. Growth test showed that isolate LBF-1-0132 has high potency to degrade pyrene compound. Isolate LBF-1-0132 is capable of degrading pyrene compounds optimally at concentration of 600 ppm and optimum cell concentration of 20. Based on 16S rDNA gene analysis, isolate LBF-1-0132 is Pseudomonas aeruginosa with 98% identity.Keywords :pyrene, marine bacteria, optimization, 16S rDNA identification
Pencirian Produksi Amilase oleh Saccaromyces cerevisiae W303A Rekombinan Thontowi, Ahmad; Puspaningsih, Ni Nyoman Tri; Hadi, Sofjan; Purkan, Purkan; Irawan, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.112 KB) | DOI: 10.14203/jbi.v3i3.3464

Abstract

ABSTRACTCharacterization of Amylase Production by Saccharomyces cerevisiae W303A Recombinants. Cloning of amylase gene from Endomycopsis fibuligera ITB.R.cc.64 into S. cerevisiae W303a can effectively increase the yeast function to digest starch directly into ethanol. Production of amylase by S. cerevisiae W303a recombinants (I and P) were done by growing in yeast peptone starch (YPS) medium. The result showed that the recombinants could be produced of amylase by gave clear zone after staining by iodium vapor. The optimum condition of production of amylase by S. cerevisiae W303a recombinants were pH 7.0, 40?C temperature incubation, and gave maximum activity after 36 hours incubation. Amylase activity of I was higher than P recombinant for these condition respectively.Key words: Characterization, amylase, S. cerevisiae W303a
Metabolisme Benzonitril oleh Flavobacterium sp. NUB 1 Sulistinah, Nunik; Sunarko, Bambang; Thontowi, Ahmad
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.945 KB) | DOI: 10.14203/jbi.v3i3.3472

Abstract

ABSTRACTMetabolism of Benzonitriles by Flavobacterium sp. NUB 1. Flavobacterium sp. NUB 1 was isolated from industrial waste of PT. Petrokimia Gresik. The bacterium was able to utilize benzonitrile and acetonitrile and propionitril as the sole source of carbon and nitrogen. Growth on benzonitrile gave higher growth rate and biomass yield than growth on acetonitrile and propionitrile. When Flavovobacterium sp. NUB1 grew on benzonitril 15 mM , the doubling time is 9 hours 54 minutes and the specific growth rate (?) was 0,07 h-1. Whole cell of Flavobacterium sp. NUB 1 could hydrolyzed aromatic and aliphatic nitriles. The bacteria isolate has ability in metabolism of acetonitrile greater than benzonitrile. Activity of nitrile hydratase and amidase are more dominant than nitrilase in metabolism of benzonitrile.Key words: Biodegradation, benzonitril, Flavobacterium sp. NUB 1, nitrile-hydratase,amidase, nitrilase
Karakterisasi Biodegradasi Senyawa Poliaromatik Dibenzothiophene Oleh Bakteri Laut Novosphingobium mathurense LBF-1-0061 Tanjung, Puspasari Noerwan; Yetti, Elvi; Thontowi, Ahmad; Suprihadi, Agung; Purwantisari, Susiana; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1056.931 KB) | DOI: 10.14203/jbi.v12i2.2894

Abstract

ABSTRACTDibenzothiophene is one of polycyclic aromatic hydrocarbon (PAH) compound containing sulfur element. This compound has toxicity, mutagenic and quiet persistent in environment. From sreening test, it was known that isolate LBF-1-0061 was potential to degrade dibenzothiophene. The objectives of this study are to study dibenzotiophene degrading capability by marine bacteria isolate LBF-1-0061 using screening test; analysis of dibenzothiophene residue by GC/MS and identifiy the isolate by molecular identification. The result of this research shown that LBF-1-0061 isolate could grow up to 100 ppm of dibenzotiophene. This isolate also presented degrading capability approximately 37.5% of dibenzotiophene in 14 days incubation. Based on partial 16S rRNA gene analysis, LBF-1-0061 was identified 99% as Novosphingobium mathurense strain SM117.Keywords: sea bacteria, biodegradation, dibenzotiofen, hydrocarbon aromatic polisiclic
Keragaman Bakteri Laut Pendegradasi Alkana dan Poliaromatik Hidrokarbon di Pulau Pari Jakarta Thontowi, Ahmad; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.283 KB) | DOI: 10.14203/jbi.v9i1.154

Abstract

Minyak mentah merupakan salah satu sumber pencemaran di lingkungan laut. Degradasi oleh bakteri memegangperanan penting dalam bioremediasinya. Sejumlah 66 bakteri laut dari Pulau Pari, Kepulauan Seribu Jakarta telahdiisolasi, dianalisa berdasarkan gen 16S rDNA, dan diuji kemampuannya dalam mendegradasi minyak. Berdasarkananalisis gen 16S rDNA diperoleh lima kelompok bakteri pendegradasi minyak, yaitu α-proteobakteria (43.6%), γ-proteobakteria (48.5 %), Flavobakteria (4.5 %), Aktinobakteria (1,5 %), dan Bacillales (1,5%). Bakteribakteritersebut mampu mendegradasi komponen minyak (senyawa alkana dan poliaromatik hidrokarbon). γ-Proteobakteria dan α-proteobakteria mempunyai peran penting dalam bioremediasi minyak di kawasan lingkunganlaut di Pulau Pari. Dari hasil tersebut membuktikan bahwa bakteri pendegradasi minyak dari Pulau Pari sangatberagam.Kata kunci: minyak, laut, bakteri, bioremediasi, alkana, poliaromatik hidrokarbon
The Optimal Conditions of Xylanase Production Using Empty Fruit Bunch Raw Biomass by Marine Isolate LBF-001 Wijaya, Hans; Thontowi, Ahmad; Rahmani, Nanik; Yopi, Yopi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.495 KB) | DOI: 10.15578/squalen.v11i3.211

Abstract

Several xylanases have already been studied. However, only a few xylanases derived from marine microorganisms has been reported. Marine bacterium LBF-001 was isolated from Pari Island Kepulauan Seribu, Indonesia. The purpose of this study is to identify isolate LBF-001 using 16S rDNA gene and to optimise the medium conditions for the xylanase production i.e. concentration of biomass, nitrogen source, pH and temperature. Based on 16S rDNA gene analyses, LBF-001 isolate has 99% similarity with Bacillus pumilus HNS70 (KF933667). Fermentation process to produce xylanases was conducted using several agricultural residues under solid-state fermentation (SSF). The optimum condition for xylanase production by B. pumilus LBF-001 was using a medium containing  2.5% empty fruit bunch and 0.6% lactose broth, at pH 6.5, temperature 30 oC, under submerged fermentation with shaking at 150 rpm for 48 h fermentation. The optimised condition resulted higher xylanase activity, i.e. 10.85 U/mL.
Evaluation of Non-Saccharomyces Cerevisiae Strains Isolated from Sea Water Against Inhibitory Compounds for Ethanol Production Thontowi, Ahmad; Putra, Filemon Jalu Nusantara; Yopi, Yopi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.849 KB) | DOI: 10.15578/squalen.v12i2.284

Abstract

An important parameter in industrial bioethanol fermentation is the resistance of yeast to osmotic pressure and inhibitor compounds. Aureobasidium pullulans LBF-3-0074 and Schwanniomyces etchellsii LBF-3-0034 are reported capable to produce ethanol. LBF-3-0034 and LBF-3-0074 are yeast strains isolated from Bali and Lombok sea water. This study aimed to evaluate characteristics of both LBF-3-0034 and LBF-3-0074 strains under the effects of glucose and inhibitor compounds. Both strains were allowed to consume glucose up to 120 mM. Then, these strains were grown with the present of several inhibitors, i.e. 5-hydroxymethyl-2-furaldehyde (5-HMF), furfural, acetic acid, formic acid, and levulinic acid. Results showed that the two yeast strains studied could grow and ferment the sugars under both osmotic and inhibitor stress conditions. As conclusion, Schwanniomyces etchellsii LBF-3-0034 and Aureobasidium pullulans LBF-3-0074 are potential for direct fermentation of lignocellulosic hydrolysate to ethanol.