. Tejasari
Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Jember

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AKTIVITAS STIMULASI KOMPONEN BIOAKTIF RIMPANG JAHE (Zingiber officinale Roscoe) PADA SEL LIMFOSIT B MANUSIA SECARA IN VITRO [Effects of Bioactive Compounds of Ginger (Zingiber officinale Roscoe) Root on B Cell Lymphocyte Function using In Vitro System

Jurnal Teknologi Dan Industri Pangan Vol 12, No 1 (2002): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

AKTIVITAS STIMULASI KOMPONEN BIOAKTIF RIMPANG JAHE (Zingiber officinale Roscoe) PADA SEL LIMFOSIT B MANUSIA SECARA IN VITRO [Effects of  Bioactive Compounds of Ginger (Zingiber officinale Roscoe) Root on B Cell Lymphocyte Function using In Vitro System]            Tejasari  1),  Fransiska R  Zakaria  2) ,  dan  Dondin Sajuthi  3) 1)    Staf Pengajar Fakultas Teknologi Pertanian, Universitas Jember 2)  Staf Pengajar Jurusan Teknologi Pangan dan Gizi, Fateta-IPB 3) Peneliti Pada Pusat Studi Satwa Primata IPB ABSTRACT The effect of two fractions of oleoresin compounds from ginger root on lymphocyte function were studied by observing B cell proliferations, which were  measured by the incorporation of 3H-thymidine during cell incubation. The doses of oleoresin compounds and its two fractions tested were 50,100, 150, and 200 mg per ml. Lymphocyte from human peripheral blood were isolated using ficoll density gradient technique, and cultured  in the presence of the compounds in RPMI-1640 medium for 4 days, with or without the addition of 3 mM paraquat as the oxidizing agent.  The results showed that the effects of oleoresin and its two fractions depended on its doses. Oleoresin and its two fractions increased B cell proliferation with  the highest activity of 456 percent at low dose of 50 mg per ml. These result showed a positive effect of oleoresin compound and its two fractions on B cell functions  at low doses. It indicated that bioactive compounds of ginger root at low  concentration have  positive effects on humoral immune response and this supports the traditional belief that ginger increases body resistance to common cold.   Key words : bioactive compound, proliferation, humoral immune responds, stress oxidative, in vitro, and  lymphocyte

EVALUASI EFEK KONSUMSI MIE BIJI ALPUKAT GORENG DAN OSENG BUNCIS SERTA DAGING KAMBING TERHADAP KADAR GULA DARAH PENYANDANG NIDDM

Jurnal AGROTEKNOLOGI Vol 6, No 1 (2012)
Publisher : Jurnal AGROTEKNOLOGI

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Abstract

Diabetes mellitus (DM) is a metabolic disease related to insulin deficient and or resistant that lead to hyperglycemic or blood glucose level higher than normal value (120 mg/100mL). Prolong hyperglikemc emerging the chronic effect such as retino, nefropati, and neuropati diabetic that decrease child growth and working performace. Balance diet with bioactive compound that stimulated pancreatic function or insulin secretion is one effort for stabilizing blood glucose level. This cross sectional research were aimed to evaluate the effect of functional diet of fried alvocado seed noodles,bean, and goat flash on blood glucose level of NIDDM subject. The functional diet consumed as breakfast for two weeks decreased blood glucose by 14.1 percent.Keywords : avocado noodle, functional diet, Diabetes mellitus(DM), blood glucose level

KONSUMSI MINUMAN NUTRAFOSIN BERISI INULIN DAN FRUKTOOLIGOSAKARIDA (FOS) MENURUNKAN KADAR TRIGLISERIDA PENDERITA DISLIPIDEMIA

Jurnal AGROTEKNOLOGI Vol 5, No 2 (2011)
Publisher : Jurnal AGROTEKNOLOGI

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Abstract

Pangan memasok zat gizi, termasuk karbohidrat yang berfungsi utama menghasilkan energi untuk aktivitas internal dan eksternal. Beberapa jenis karbohidrat, seperti inulin (fruktan) dan fruktooligosakarida (FOS) telah terbukti mampu memodifikasi proses fisiologis dan biomiawi, khususnya metabolisme asam lemak dan kolesterol. Kenyataan ini menunjukkan bahwa kedua senyawa karbohidrat tersebut secara aktif biologis berpengaruh terhadap profil lipida darah, termasuk trigliserida (TAG). Kedua senyawa tersebut telah diklaim sebagai bahan pangan fungsional. Efek fungsional sehat inulin dan FOS akan dirasakan jika individu cukup konsumsi energi dan zat gizinya. Di Indonesia, uji klinis efek sehat bahan pangan fungsional pada manusia sulit dilakukan karena tidak dapat mengontrol seutuhnya konsumsi pangan subjek. Penelitian ini menggunakan penderita dislipidemia yang memenuhi syarat inklusi subjek, dan bersedia (informed consent) untuk mengikuti semua prosedur perlakuan yang telah disepakati, selama 21 hari, dengan pola konsumsi seperti biasanya. Subjek dislipidemia adalah pasien rawat jalan Rumah Sakit Subandi Jember, sehingga telah mendapatkan konsultasi gizi baik dari dokter ahli penyakit jantung maupun dari bagian gizinya. Tujuan penelitian ini adalah mengevaluasi efek penurunan kadar trigliserida akibat asupan inulin dan FOS dalam minuman Nutrafosin pada penderita dislipidemia berpola konsumsi seperti biasa. Data konsumsi pangan diperoleh dengan metoda 24 jam Recall Method. Kebutuhan energy subjek dislipidemi ditentukan berdasarkan berat badan relative (BBR). Jika BBR<90% maka kebutuhan energy sebesar 40-60 Kal/kg BB. Jika BBR 90-100% maka kebutuhan energy sejumlah 30 Kal/kg BB. Jika BBR>110% maka kebutuhan energy 20 Kal/kg BB. Bila BBR>120% maka kebutuhan energinya sebesar 15 Kal/kg BB. Lalu, kebutuhan energy dibandingkan dengan asupan energinya. Penelitian eksperimental klinik (clinical trial) dengan metode uji klinis dan desain pararel matched pairs. Sampel yang dikehendaki (intended sampel) diambil dari accesible population secara non-probability dengan consecutive sampling. Minuman Nutrafosin berisi 2 g bubuk inulin dan 50 ml bahan cair FOS diberikan secara single blinding placebo designed kepada 16 subjek dislipidemia untuk dikonsumsi setiap hari 1 gelas secara oral. Pengukuran kadar TAG dilakukan sebelum (0 hari) dan terakhir (hari ke-22). Perbedaan kadar TAG sebelum dan sesudah intervensi, kelompok placebo dan perlakuan, dianalisis dengan uji statistic T-test berpasangan Confidence Interval 95%. Hasil penelitian membuktikan bahwa konsumsi minuman Nutrafosin oleh subjek dislipidemia selama 21 hari menurunkan kadar trigliserida secara nyata. (Thitung= 1,108 < TTabel = 2,365). Berdasarkan temuan ini untuk hasil yang lebih optimal, disarankan pada penderita dislipidemia untuk mengurangi asupan energy dari karbohidrat agar tidak lebih dari 50% total energy AKG, dan meningkatkan asupan serat larut air dari kelompok pangan bebuahan, dan tidak larut air (seperti selulosa) dari sayuran minimal 30 g serat per hari. Terkait hal tersebut, diperlukan daftar kadar serat pangan berbagai jenis pangan nabati Indonesia, agar dapat digunakan oleh masyarakat sebagai acuan perencanaan konsumsi pangan sumber serat, terutama bagi penderita dislipidemia.Kata kunci: Inulin, Fruktooligosakarida (FOS), trigliserida, dislipidemia, serat pangan

MUTU GIZI DAN TINGKAT KESUKAAN MINUMAN KOPI DEKAFOSIN INSTAN

Jurnal AGROTEKNOLOGI Vol 4, No 1 (2010)
Publisher : Jurnal AGROTEKNOLOGI

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Abstract

Reducing caffeine was meant for improving health effect of drinking coffee. Meanwhile supplementation of functional food ingredient such inulin and fructooligosaccharides (FOS) in coffee beverage is expected for emerging health value of decaff coffee. Decaffosin instant coffee was formulated from decaffeinated coffee powder enriched with 27 % w/w inulin powder and 7 % fructooligosacharides (FOS). This study aims to identify nutritional quality and to evaluate the effect of inulin and FOS addition at the same time on preference level of Dekafosin instan coffee. The result of showed that Dekafosin instan coffee contain 5.04 % of water and 2.3 % of mineral. Total reducing sugar, sucrose, glucose, fructose and soluble dietary fiber content of the beverage are 30.83, 0.47, 2.8, 94.33 and 98.1 percentage, respectively. Meanwhile, caffeine and inulin content of the the beverage are 1.23 and 28.15 percentage. The statistical analysis proved that decaffeination and the supplementation of inulin and FOS did not influence on the preference level of Dekafosin instant coffee, as showing by the following value : preference level of colour (x² count = 17.397 < x² table = 18.549), aroma (x² count = 5.563 > x² table = 4.168) and taste (x² count = 11.413 < x² table = 14.684) wich has a significance value (p) > 5 percent. Therefore, based on this analysis data it can be concluded an addition of inulin and FOS did not influence preference level toward Dekafosin instant coffee beverage.Keywords : decaffeinated coffee, inulin, FOS, Dekafosin instant coffee beverage, nutritional quality, preference level

AKTIVITAS STIMULASI KOMPONEN BIOAKTIF RIMPANG JAHE (Zingiber officinale Roscoe) PADA SEL LIMFOSIT B MANUSIA SECARA IN VITRO [Effects of Bioactive Compounds of Ginger (Zingiber officinale Roscoe) Root on B Cell Lymphocyte Function using In Vitro System

Jurnal Teknologi Dan Industri Pangan Vol 12, No 1 (2002): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Original Source | Check in Google Scholar | Full PDF (238.275 KB)

Abstract

The effect of two fractions of oleoresin compounds from ginger root on lymphocyte function were studied by observing B cell proliferations, which were  measured by the incorporation of 3H-thymidine during cell incubation. The doses of oleoresin compounds and its two fractions tested were 50,100, 150, and 200 mg per ml. Lymphocyte from human peripheral blood were isolated using ficoll density gradient technique, and cultured  in the presence of the compounds in RPMI-1640 medium for 4 days, with or without the addition of 3 mM paraquat as the oxidizing agent.  The results showed that the effects of oleoresin and its two fractions depended on its doses. Oleoresin and its two fractions increased B cell proliferation with  the highest activity of 456 percent at low dose of 50 mg per ml. These result showed a positive effect of oleoresin compound and its two fractions on B cell functions  at low doses. It indicated that bioactive compounds of ginger root at low  concentration have  positive effects on humoral immune response and this supports the traditional belief that ginger increases body resistance to common cold.

KONSUMSI MINUMAN NUTRAFOSIN BERISI INULIN DAN FRUKTOOLIGOSAKARIDA (FOS) MENURUNKAN KADAR TRIGLISERIDA PENDERITA DISLIPIDEMIA

Jurnal AGROTEKNOLOGI Vol 5, No 2 (2011)
Publisher : Jurnal AGROTEKNOLOGI

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Abstract

Food contains nutrients, including carbohydrate which has major role in energy production needed for internal and external activities. Several kinds of carbohydrate, such as inulin (fruktan) and fructooligosacharides (FOS) were proven be able to modify physiologic and biochemical process, specifically fatty acids and cholesterol metabolism. These fact showed that this both carbohydrate compounds biologically active in influencing blood lipid profile, including tryasilglycerol (TAG) or tryglycerides. Both compounds have been claimed as functional food ingredients. Health effect of inulin and FOS will be emerged if the subject has enough energy and nutrient intake. In Indonesia, clinical trial of health effect of functional food ingredient on human is difficult to be done because of problem in controlling entirely the subject food consumption. This study was done on dyslipidemic subject that fulfill inclusion requirement and willing (informed consent) to follow all experiment procedures for 21 days, with their daily food consumption pattern as usual. Dyslipidemic subjects are out patient in Subandi Hospital in Jember. Therefore, they have been given nutrition consultation from specialized medical doctor in cardiovascular diseases and from nutrition unit as well. This experiment aims to evaluate the effect of inulin and FOS intake from Nutrafosin beverage consumption on tryglycerides level of dyslipidemic whom their consumption pattern was as usual. Food consumption data was gained through 24 hours Recall Method for 3 days. Energy requirement was determined based on relative body weight (RBW). This experimental study was a clinical trial as a pararel matched pairs design. The intended sample were choosen from accesible population thru non-probability and consecutive sampling. One cup of Nutrafosin beverage contained 2 g inulin powder and 50 ml FOS that given by single blinding placebo designed to 16 dislipidemic subject for 21 days consumption orally. Tryglycerides level measurement was done at 0 days and 22th days. The tryglycerides level difference between before and after treatment from placebo and treatment groups was analyzed using statistical T-test pair at 95 % confidence interval. The result showed that Nutrafosin beverage consumption by dyslipidemic for 21 days decreased trygycerides by 18.5 percent, significantly (Tc= 1,108 < Ttab = 2,365).Keywords: inulin, fructooligosacharide (FOS), tryglycerides, dysl ipidemic, dietary fibre consumption pattern

MUTU GIZI DAN TINGKAT KESUKAAN MINUMAN KOPI DEKAFOSIN INSTAN

JURNAL AGROTEKNOLOGI Vol 4 No 01 (2010)
Publisher : Faculty of Agricultural Technology, University of Jember

Show Abstract | Original Source | Check in Google Scholar | Full PDF (661.406 KB)

Abstract

Reducing caffeine was meant for improving health effect of drinking coffee. Meanwhile supplementation of functional food ingredient such inulin and fructooligosaccharides (FOS) in coffee beverage is expected for emerging health value of decaff coffee. Decaffosin instant coffee was formulated from decaffeinated coffee powder enriched with 27 % w/w inulin powder and 7 % fructooligosacharides (FOS). This study aims to identify nutritional quality and to evaluate the effect of inulin and FOS addition at the same time on preference level of Dekafosin instan coffee. The result of showed that Dekafosin instan coffee contain 5.04 % of water and 2.3 % of mineral. Total reducing sugar, sucrose, glucose, fructose and soluble dietary fiber content of the beverage are 30.83, 0.47, 2.8, 94.33 and 98.1 percentage, respectively. Meanwhile, caffeine and inulin content of the the beverage are 1.23 and 28.15 percentage. The statistical analysis proved that decaffeination and the supplementation of inulin and FOS did not influence on the preference level of Dekafosin instant coffee, as showing by the following value : preference level of colour (x² count = 17.397 < x² table = 18.549), aroma (x² count = 5.563 > x² table = 4.168) and taste (x² count = 11.413 < x² table = 14.684) wich has a significance value (p) > 5 percent. Therefore, based on this analysis data it can be concluded an addition of inulin and FOS did not influence preference level toward Dekafosin instant coffee beverage.Keywords : decaffeinated coffee, inulin, FOS, Dekafosin instant coffee beverage, nutritional quality, preference level