Kustiariyah Tarman
Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor

Published : 32 Documents
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Journal : Jurnal Pengolahan Hasil Perikanan Indonesia

Aktivitas Antibakteri Ekstrak Kapang Laut Nodulisporium sp. KT29 terhadap Vibrio harveyi Hariati, Sri; Wahjuningrum, Dinamella; Yuhana, Munti; Tarman, Kustiariyah; Effendi, Irzal; Saputra, Fazril
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21, No 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.422 KB) | DOI: 10.17844/jphpi.v21i2.22855

Abstract

Nodulisporium sp. KT29 merupakan salah satu kapang endofit yang diisolasi dari alga merah Eucheuma edule, tidak bersifat toksik, dan bersifat antibakteri. Penelitian ini bertujuan mengevaluasi aktivitas antibakteri ekstrak kapang laut Nodulisporium sp. KT29 terhadap Vibrio harveyi. Penelitian dilakukan dengan beberapa tahapan yaitu kultivasi kapang, ekstraksi senyawa aktif, pengujian aktivitas antibakteri, kromatografi lapis tipis (KLT), bioautografi, serta pengamatan kerusakan sel bakteri Vibrio harveyi. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etil asetat dan dipekatkan dengan rotary evaporator. Ekstrak Nodulisporium sp. KT29 diuji terhadap bakteri Vibrio harveyi pada konsentrasi 0,125; 0,25; 0,5; 1 dan 2 mg/disc. Ekstrak Nodulisporium sp. KT29 membentuk zona hambat tertinggi pada konsentrasi 2 mg sebesar 45,33±0,71 mm. Hasil uji KLT ekstrak Nodulisporium sp. KT29 menunjukkan adanya 7 spot retardation factor (Rf) dengan tiga komponen warna yaitu cokelat, kuning dan ungu. Uji bioautografi menghasilkan 2 spot zona hambat yaitu 16 mm dan 13 mm pada Rf 0,94 dan 0,14. Pengamatan menggunakan SEM menunjukkan bahwa kerusakan pada morfologi sel Vibrio harveyi dengan pemberian ekstrak Nodulisporium sp. KT29 yang ditandai dengan lisis.
Characterization and Photoprotector Activity of Endophytic Fungal Pigments from Coastal Plant Sarang Semut (Hydnophytum formicarum) Sibero, Mada Triandala; Tarman, Kustiariyah; Hanif, Novriyandi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19, No 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.119 KB) | DOI: 10.17844/jphpi.v19i1.12512

Abstract

Endophytic fungus RS3 isolated from coastal plant sarang semut (Hydnophytum formicarum) produced extracellular black pigment. The aims of this research were to obtain the pigment, to characterize and to determine the photoprotector activity. This research was conducted into several steps, that were determination of the best precipitating agent, characterization using instrument and solubility analysis, and analysis of Sun Protection Factor (SPF). Results showed the pigment was precipitated using acid solvent with pH ? 2.5. Functional groups of pigment were hydroxyl, aromatic ring, phenol and amine. According to its characteristics, black pigment produced by RS3 isolate was proposed as melanin. The photoprotector analysis showed the SPF value was 11.33.
Antibacterial and Antioxidant Activity of Green Algae Halimeda gracilis from Seribu Island District Basir, Abdul; Tarman, Kustiariyah; Desniar, D.
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20, No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.455 KB) | DOI: 10.17844/jphpi.v20i2.17507

Abstract

Seaweeds have ecological functions as primary producers in marine waters. It also has an important economic value as a producer of hydrocolloids (alginate, agar and carrageenan) that is used in various industries of food and pharmaceuticals. This study aimed to determine the antibacterial and antioxidant activity of green algae Halimeda gracilis. The study was conducted in several stages, sample collection and preparation, extraction of bioactive compound, fractionation, antibacterial and antioxidant test, and phytochemical. Extraction was done by maceration method using methanol and concentrated by rotary evaporator. The methanol extracts of H. gracilis were tested against Staphylococcus aureus and Escherichia coli. Methanol extract of H. gracilis formed inhibition zone against the test bacteria with diameter of inhibition zone was 10 mm and 6 mm, respectively. After liquid-liquid partition (water: ethyl acetate), inhibition zone was only seen in the ethyl acetate fraction of H. gracilis with diameter of inhibition zone was 6 mm and 7.50±1.71 mm, respectively. Antioxidant test methanol extracts and ethyl acetate fractions of H. gracilis each show IC50 value of 290.49 ppm and 375.50 ppm. Phytochemical test showed methanol extract of H. gracilis contains phenols and steroids.
Characterization and Photoprotector Activity of Endophytic Fungal Pigments from Coatal Plant Sarang Semut (Hydnophytum formicarum) Sabero, Mada Triandala; Tarman, Kustiariyah; Hanif, Novriyandi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19, No 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Departement of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (39.104 KB) | DOI: 10.17844/jphpi.v19i1.11685

Abstract

Isolate endophytic fungal RS3 from smooth ant plants (Hydnophytum formicarum) produced blackpigment. The aims of this research were to obtain the pigment, to characterize and to determine thephotoprotector activity. This research was consisted into several steps, there were determined the bestprecipitating agent, characterization using instrument and solubility analysis, and analysis of Sun ProtectionFactor (SPF). Results showed the pigment was precipitated using acid solvent with pH ≤ 2,5. Functionalgroups of pigment pellet were hydroxy, aromatic ring, phenol and amine. According to characteristic, blackpigment produced by fungal RS3 proposed as melanin. The photoprotector analysis showed SPF the valuewas 11.33.Keywords: endophyte, melanin, pigment, photoprotector, SPF
Extraction and Characterization of Collagen from Sea Cucumber Flesh Alhana, A.; Suptijah, Pipih; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 18, No 2 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (624.02 KB) | DOI: 10.17844/jphpi.v18i2.10610

Abstract

Sea cucumber (Stichopus variegatus) is one of the Echinodermata phylum that growsalong Indonesian coastal. Sea cucumber is potential source of collagen. The purposes of thisresearch were to determine the optimal concentration of NaOH and CH3COOH solutionin collagen production and analyze the physicochemical characteristics of collagen fromS. variegatus. Yield of the collagen was 1.5% (based on wet weight basis), produced bypretreatment with NaOH 0,30%, hydrolysis with CH3COOH 0.10% and extracted usingdistilled water. Protein, moisture, and ash content of the collagen was 67.68%, 13.64%,and 4.15%, respectively. Collagen was extracted using distilled water at 45°C during 2hand still had triple helix structure ; pH 7.37 ; melting temperature 163.67°C and whiteness69.25%. The major amino acid content of collagen were glycine, alanine, proline andglutamic acid.Keywords: NaOH, collagen, Stichopus variegatus, FTIR.
Toxicity Sub chronic Water Extract Meretrix meretrix Linnaeus In Vivo on Sprague dawley Rats Apriandi, Azwin; Tarman, Kustiariyah; Sugita, Purwantiningsih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19, No 2 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.82 KB) | DOI: 10.17844/jphpi.v19i2.13464

Abstract

Meretrix meretrix is one of the shells of sea water are widely utilized by people as food. This clamalso has many properties and benefits, so in this study tested the effect of the water extract of Meretrixmeretrix against blood chemistry profile Sprague Dawley rats with the method (OECD 413: 2009). Based onobservations obtained growth, feed intake, weight of liver and kidney in normal conditions. Levels of urea,creatinine, cholesterol between the control mice treated with A/0.1 and A/1 were not significantly different(p> 0.05) while the levels of bilirubin and albumin between control mice treated with A/0.1 and A/1 resultssignificantly different (p<0.05), but all blood chemistry parameters tested is still in the normal category.
Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans) against Vibrio sp. Isolated From Mud Crab (Scylla sp.) Imra, Imra; Tarman, Kustiariyah; Desniar, Desniar
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19, No 3 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.567 KB) | DOI: 10.17844/jphpi.v19i3.15079

Abstract

AbstractNipah (Nypa fruticans) is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf) that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah?s leaf extract was more effective (22,5 ?g/mL) than nipah?s fruit extract (415 ?g/mL). This activity to be classified to the strong antioxidant activity (IC50<50 ?g/mL). The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah?s leaf was toxic with toxicity value is 663,598 ?g/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah?s leaf.
Potensi Kolagen Teripang Emas sebagai Inhibitor Tirosinase Safithri, Mega; Setyaningsih, Iriani; Tarman, Kustiariyah; Yuhendri, Vitriyanna Mutiara; Meydia, Meydia
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21, No 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.598 KB) | DOI: 10.17844/jphpi.v21i2.23085

Abstract

Kolagen memiliki aktivitas antioksidan dan mampu menghambat aktivitas tirosinase pada prosesmelanogenesis, salah satu biota laut yang dapat dijadikan sumber kolagen alternatif adalah teripang.Penelitian ini bertujuan menentukan karakteristik kolagen dan aktivitas penghambatan enzim tirosinaseoleh kolagen teripang emas (Stichopus hermanii). Penghilangan protein non kolagen dilakukan dengancara perendaman daging teripang menggunakan NaOH 0,1 M selama 48 jam. Isolasi kolagen dilakukanmenggunakan asam asetat 0.5 M selama 48 jam. Analisis gugus fungsi kolagen dilakukan menggunakanFTIR dan analisis aktivitas tirosinase dilakukan menggunakan spektrofotometer. Kadar protein nonkolagen yang diperoleh yaitu 0,090 mg/mL dan kolagen teripang emas memiliki rendemen 0,66%. Kolagenteripang memiliki nilai pH 6,91, gugus fungsi khas amida A (3379,29 cm-1), amida B (2924,09 cm-1), amidaI (1681,93 cm-1), amida II (1568,13 cm-1), dan amida III (1269,16 cm-1). Aktivitas penghambatan tirosinasemenunjukkan bahwa kolagen teripang emas memiliki nilai IC50 5610 ppm. Kolagen teripang emas belummampu menghambat aktivitas tirosinase.
Anticancer Activity from Active Fraction of Sea Cucumber Putram, Nurul Mutia; Setyaningsih, Iriani; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20, No 1 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1191.863 KB) | DOI: 10.17844/jphpi.v20i1.16399

Abstract

Sea Cucumber Holothuria atra is one of marine organisms has been used as a new source of novel bioactive compounds. Many of them have been used as the lead compounds in discovery of new anticancer drugs. The objective of this study was to determine the active fractions of sea cucumber (H. atra) which have anticancer activity. H. atra was macerated using ethanol and the extract was freezedried using a freeze dryer. The crude extract was partitioned using n-hexane, ethyl acetate, and methanol-water (3:1:1:1). Cytotoxicity test was performed using HeLa (cervic cancer) cell line and MCF-7 (breast cancer) cell line based on the MTT assay. The crude extract of H. atra showed the best cytotoxic activity against HeLa cells (IC50 = 12.48 µg/mL) and MCF-7 cells (IC50 = 17.90 µg/mL). The toxicity tests showed the IC50 value of the n-hexane fraction, ethyl acetate fraction, and methanol-water fraction against HeLa cells HeLa (IC50 = 76.45 µg/mL; 77.95 µg/mL;  14.27 µg/mL) and MCF-7 cells (IC50 = 58.50 µg/mL; 59.59 µg/mL; 14.33 µg/mL).
KANDUNGAN KOMPONEN BIOAKTIF DAN AKTIVITAS ANTIMIKROB EKSTRAK BINTANG LAUT (Culcita schmideliana) Tarman, Kustiariyah; Prestisia, Hana Nurullita; Setyaningsih, Iriani; Meydia, Meydia; Yogiara, Yogiara; Hwang, Jae-Kwan
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15, No 3 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia 15 (3)
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.925 KB) | DOI: 10.17844/jphpi.v15i3.21418

Abstract

Bintang laut Culcita schmideliana merupakan salah satu spesies dari kelas Asteroidea yang memiliki potensi sebagai antimikrob. Tujuan penelitian ini adalah untuk menentukan komponen bioaktif dan aktivitas antimikrob dari bintang laut C. schemideliana. Penelitian ini dilakukan melalui beberapa uji, yaitu uji komponen fitokimia, uji aktivitas antimikrob, dan fraksinasi dengan kromatografi lapis tipis, serta bioautografi. Hasil uji fitokimia secara kualitatif menunjukkan bahwa ekstrak metanol mengandung alkaloid, steroid, flavonoid, saponin, dan ninhidrin, sedangkan ekstrak n-heksan dan etil asetat mengandung steroid dan saponin. Aktivitas antimikrob terbesar ditunjukkan oleh ekstrak etil asetat dengan konsentrasi 2 mg yaitu sebesar 16 mm. Fraksinasi KLT diidentifikasi memiliki 7 bercak pada lampu sinar UV ? 254 nm dan UV ? 366 nm menghasilkan 1 bercak. Pengamatan bioautografi terhadap bakteri B. subtilis menghasilkan zona hambat sebesar 7 mm pada Rf = 0,02 dan pada Rf = 0,62 menghasilkan zona hambat sebesar 3 mm.