Kustiariyah Tarman
Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor

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Kandungan Senyawa Aktif Spirulina platensis yang Ditumbuhkan pada Media Walne dengan Konsentrasi NaNO3 Berbeda Notonegoro, Hartoyo; Setyaningsih, Iriani; Tarman, Kustiariyah
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): Desember 2018
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v13i2.555

Abstract

AbstrakSpirulina platensis merupakan sumber makanan organik  yang mengandung protein tinggi dengan asam amino yang seimbang. Spirulina juga memiliki kandungan senyawa aktif yaitu fikosianin dan flavonoid. Senyawa aktif tersebut pada umumnya memiliki aktivitas yang potential sebagai suplemen dan sediaan bahan aktif pada pangan fungsional. Salah satu faktor yang mempengaruhi kandungan fikosianin dan flavonoid dari mikroalga adalah nutrisi yang digunakan dalam media pertumbuhan sehingga perlu dilakukan kajian mengenai pengaruh komposisi media terhadap kandungan fikosianin dan flavonoid. Penelitian ini bertujuan untuk menentukan pengaruh variasi kandungan NaNO3 pada media pertumbuhan terhadap kandungan fikosianin dan flavonoid S. platensis; serta menentukan konsentrasi NaNO3 terbaik pada media Walne untuk menghasilkan biomassa S. platensis dengan kandungan fikosianin dan flavonoid  tertinggi. Penelitian ini terdiri dari 2 tahapan yaitu kultivasi S. platensis; dan ekstraksi fikosianin, flavonoid, dan komponen aktif lainnya. Kultur S. platensis menggunakan media Walne dengan variasi kandungan NaNO3 yaitu masing-masing sebesar 80, 100 dan 120 g selama 11 hari. Total protein dan nitrogen tertinggi diperoleh pada perlakuan 100 g NaNO3 masing-masing sebesar 44,30% dan 7,09%. Biomassa S. platensis setiap perlakuan NaNO3 mengandung flavonoid, steroid, fenol dan saponin. Konsentrasi dan rendemen fikosianin terbaik diperoleh pada perlakuan 80 g NaNO3 sebesar 1,32 mg/ml dan 32,93%.Total flavonoid ekstrak S. platensis tertinggi diperoleh pada perlakuan 80 g NaNO3 sebesar 16,56%. S. platensis terpilih adalah perlakuan NaNO3 80 g karena menghasilkan kandungan senyawa aktif flavonoid dan fikosianin tertinggi. ncentration. The Active Compounds of  Spirulina  platensis Grown on Walne Media with Different NaNO3 ConcentrationsAbstractSpirulina platensis is a source of organic foods that contain high protein with balanced amino acids. Spirulina also contains active compounds of phycocyanin and flavonoids. The active compound communly to have potential activities as supplement and functional food. One of the factors that affects the content of phycocyanin and flavonoids from microalgae is the nutrients used in growth media. So that it is necessary to study the effect of media composition on the content of phycochyanin and flavonoids. This study aimed to determine the effect of various concentrations of NaNO3 on growth media on the content of flavonoids and phycocyanin and determine the best NaNO3 concentration in Walne media that produce S. platensis biomass with the highest phycocyanin and flavonoid contents. This study was run in two steps that were cultivation of S. platensis and extraction of phycocyanin, flavonoid, and other active components. S. Platensis were cultured in Walne media with various NaNO3 concentrations that were 80, 100 and 120 g for 11 days. The highest total protein and nitrogen were obtained from 100 g NaNO3 treatment which were 44.30% and 7.09% respectively. The biomass of S. platensis for each of NaNO3 treatment contains flavonoids, steroids, phenols and saponins. The best concentration and yield of phycocyanin were obtained from the treatment of 80 g NaNO3 of 1.32 mg/ml and 32.93% respectively. The highest total flavonoids of S. platensis extract were obtained at 80 g NaNO3 treatment of 16.56%. The best treatment to grow S. platensis which produced highest active content of phycocyanin and flavoniud was gained from 80 g NaNO3 medium
FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] Inthe, Mita Gebriella; Tarman, Kustiariyah; Safithri, Mega
Jurnal Teknologi Dan Industri Pangan Vol 25, No 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.397 KB) | DOI: 10.6066/8301

Abstract

FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] Mita Gebriella Inthe1), Kustiariyah Tarman1)* dan Mega Safithri2) 1) Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor 2) Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor   Diterima 22 Juli 2013 / Disetujui 03 Maret 2014 ABSTRACT Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells.
ISOLATION AND SCREENING OF ENDOPHYTIC MARINE FUNGI FOR CELLULASE PRODUCTION Andhikawati, Aulia; Oktavia, Yulia; Ibrahim, Bustami; Tarman, Kustiariyah
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 6, No 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Cellulolytic fungi are potentially useful in biotechnology. Decomposition of substrates containing cellulose can be applied in bioethanol and enzymes production. Fungi are one group of microorganisms that can decompose cellulase materials.  This study aimed to obtain potential cellulolytic fungi isolated from marine habitat. Screening of the fungi was performed using CBM media with different salinity. Six isolates were tested quantitatively for cellulolytic activity using congo red staining. Cellulolytic index of algicolous and manglicolous fungi were lower than those of EN isolate, Veronea sp. KT19, and sponge associate (SMH). Fungi cultured in freshwater and seawater media possessed higher cellulolytic index in compared to those of cultures in 3% NaCl medium. However, freshwater and seawater media showed no significant effect. Cellulolytic index of EN isolate, Veronaea sp. KT19, and SMH after six days of incubation were 1.357; 1.267, and 1.278, respectively. All six isolates potentially produced cellulase in all medium tested. Keywords: Cellulolytic, cellulose, endophytes, enzyme
CHARACTERIZATION OF CRUDE CELLULASE OF SEAGRASS ENDOPHYTIC FUNGUS Oktavia, Yulia; Andhikawati, Aulia; Nurhayati, Tati; Tarman, Kustiariyah
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 6, No 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

In this study, cellulase was produced by endophytic fungus isolated from seagrass. Substrate used for incubating the fungus was the waste of agar industry. The objectives of this study were to optimize cellulase production and to characterize the highest activity of fungal crude cellulase. In our previous study, the EN isolate (isolated from Enhalus sp.) showed the highest cellulolytic index. Therefore, in this research we focused on cellulase activity of the isolate. Cellulase activity was determined based on endoglucanase activity, total cellulase activity, and β-glucosidase activity. The highest activity was then used to determine cellulase activity in enzyme characterization. The fungus was cultured in different concentration of agar extraction algal wastes. The fungal culture was incubated for 3-21 days with 120 rpm orbital shaker. The results showed that endoglucanase activity was 0.019-0.031 U/mL, total cellulase activity was 0.007-0.013 U/mL, and β-glucosidase activity was 0.00012-0.00361 U/mL. The highest endoglucanase and total cellulase activity were obtained from the fungal culture after 9 days incubation, β-glucosidase was obtained from the fungal culture after 15 days incubation with 1.5% of algal waste as substrate. The optimum pH and temperature were determined as 4 and 60 oC, maximum reaction rate (Vmax) and Michaelis-Menten constant (Km) for endoglucanase activity was determined as 0.044 U/mL and 0.103% respectively. Keywords: algal waste, cellulase, endophytes, enzymes, seagrass
AKTIVITAS ANTIBAKTERI PROTEIN KAPANG Xylaria psidii KT30 TERHADAP Escherichia coli DAN Bacillus subtilis [Antibacterial Activity of Protein Fungus Xylaria psidii KT30 Escherichia coli and Bacillus subtilis] Munandar, Aris; Mustopa, A. Zaenal; Tarman, Kustiariyah; Nurhayati, Tati
Jurnal Teknologi dan Industri Pangan Vol 25, No 2 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.636 KB) | DOI: 10.6066/jtip.2014.25.2.146

Abstract

Previous research shows that extracellular protein of an algicolous fungus Xylaria psidii KT30 inhibited Bacillus pumilus, Listeria sp., Salmonella typhi, Staphylacoccus aureus, and Pseudomonas sp. with an average clear zone diameter of 7 mm. To enhance the potent antibacterial activity of extracellular protein from Xylaria psidii KT30, the present research demonstrated fungal growth optimization and purification of its secreted extracellular protein. The fungal growth optimization was performed following NaCl concentration and harvest day variations. The protein was precipitated using ammonium sulphate at 60%-90% saturation range and was purified through gel chromatography filtrationusing Sephadex G-50, eluted with 30% aq methanol. The active fraction possesing antibacterial activity was then determined resulting supernatant, pellet, and protein fraction.The fungal was optimally obtained after 15 days cultivation using freshwater.The highest protein yield was 1.67%, resulted over 90% saturation. Fractions 11 and 12 were the most active against Escherichia coli dan Bacillus subtiliswith clear zone diameter of 8 mm.Three bands of those fractions were detected through SDS-PAGE analysis, revealing molecular weights of 23.42, 20.09, and 14.33 kDa.  
Profile of amino acid, fatty acid, and mineral content of Tambelo (Bactronophorus sp.) from Kendari, Southeast Sulawesi Riviani, Riviani; Purwaningsih, Sri; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19, No 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.295 KB) | DOI: 10.17844/jphpi.v19i1.11696

Abstract

Public coastal had trust natural material as medicine became one of the most important things inthe discovery of medicine latest. Papua, Belitung, and Kendari coastal communities believed that tambelo(Bactronophorus sp.) can treat various kinds of diseases like lumbago, rheumatism, cough, flu, malaria, andimprove production of the breast milk, increase appetite, and vitality of man. It was important to know whatcourse content of tambelo. Tambelo could be examined amino acids, fatty acids, and mineral contents. Thehighest essential amino acids in tambelo were leucyne, lysine, and valin of 0.57%, 0.39%, 0.36%. The highestnon essential amino acid was alanin, glutamic acid, and aspartic acid of 1.24%, 1.09%, and 0.78%. Barrierof amino acid was histidine. Fatty acid total of tambelo was 29.52%, whereas the fatty acid compositionsconsist of 10.09% saturated fatty acid (SAFA) was 14.37% monounsaturated fatty acid (MUFA) was 5.06%polyunsaturated fatty acids (PUFAs). Among them, those occuring in the highest proportions were palmaticacid (4.49%), oleic acid (5.73%), palmitoleic acid (4.96%), and Aracidic acid (1.88%). Tambelo had n6/n3ratio of 1.84. Tambelo had mineral contents as Natrium of 1144000 mg/kg, calcium of 17000 mg/kg, Kaliumof 21000 mg/kg, magnesium of 13000 mg/kg, phosphor of 1900 mg/kg, cadmium < 0.24 mg/kg, and lead<1.25 mg/kg.Keyword : amino acids, Bactronophorus sp., fatty acids, mineral contents, tambelo
Characterization and Photoprotector Activity of Endophytic Fungal Pigments from Coatal Plant Sarang Semut (Hydnophytum formicarum) Sabero, Mada Triandala; Tarman, Kustiariyah; Hanif, Novriyandi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19, No 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Departement of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (39.104 KB) | DOI: 10.17844/jphpi.v19i1.11685

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Isolate endophytic fungal RS3 from smooth ant plants (Hydnophytum formicarum) produced blackpigment. The aims of this research were to obtain the pigment, to characterize and to determine thephotoprotector activity. This research was consisted into several steps, there were determined the bestprecipitating agent, characterization using instrument and solubility analysis, and analysis of Sun ProtectionFactor (SPF). Results showed the pigment was precipitated using acid solvent with pH ≤ 2,5. Functionalgroups of pigment pellet were hydroxy, aromatic ring, phenol and amine. According to characteristic, blackpigment produced by fungal RS3 proposed as melanin. The photoprotector analysis showed SPF the valuewas 11.33.Keywords: endophyte, melanin, pigment, photoprotector, SPF
FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] Inthe, Mita Gebriella; Tarman, Kustiariyah; Safithri, Mega
Jurnal Teknologi dan Industri Pangan Vol 25, No 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.397 KB) | DOI: 10.6066/jtip.2014.25.1.39

Abstract

FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa[Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]Mita Gebriella Inthe1), Kustiariyah Tarman1)* dan Mega Safithri2)1) Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor2) Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 22 Juli 2013 / Disetujui 03 Maret 2014ABSTRACT Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells.
Antibacterial activity of Stichopus hermanii and Stichopus variegatus Methanol Extract Susanto, Hendra; Safithri, Mega; Tarman, Kustiariyah
Current Biochemistry Vol 4, No 2 (2017)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (966.659 KB) | DOI: 10.29244/%p

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Since 500 years ago sea cucumber has been used as wound healing medicine. Sea cucumbers are thought to function as a cell growth factor and have the potential as an antibacterial agent. This study aims to determine whether the methanol extract of sea cucumber has antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa so that it can be used to help diabetic wound healing of people infected with the same bacteria. The extraction method used was maceration with methanol to dried frozen simplicia. The simplicia was obtained by freeze drying to liquid preparation of heated fresh sea cucumber. The antibacterial activity test was performed using well diffusion method. There were no any antibacterial activity found in the two methanol extracts of sea cucumber.
Extraction and Characterization of Collagen from Sea Cucumber Flesh Alhana, A.; Suptijah, Pipih; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 18, No 2 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (624.02 KB) | DOI: 10.17844/jphpi.v18i2.10610

Abstract

Sea cucumber (Stichopus variegatus) is one of the Echinodermata phylum that growsalong Indonesian coastal. Sea cucumber is potential source of collagen. The purposes of thisresearch were to determine the optimal concentration of NaOH and CH3COOH solutionin collagen production and analyze the physicochemical characteristics of collagen fromS. variegatus. Yield of the collagen was 1.5% (based on wet weight basis), produced bypretreatment with NaOH 0,30%, hydrolysis with CH3COOH 0.10% and extracted usingdistilled water. Protein, moisture, and ash content of the collagen was 67.68%, 13.64%,and 4.15%, respectively. Collagen was extracted using distilled water at 45°C during 2hand still had triple helix structure ; pH 7.37 ; melting temperature 163.67°C and whiteness69.25%. The major amino acid content of collagen were glycine, alanine, proline andglutamic acid.Keywords: NaOH, collagen, Stichopus variegatus, FTIR.