C. Talib
Center Research Institute of Animal Sciences, Jl. Raya Pajajaran Kav. E59, Bogor

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COMPARISON ON ACCURACY OF LOGISTIC, GOMPERTZ AND VON BERTALANFFY MODELS IN PREDICTING GROWTH OF NEW BORN CALF UNTIL FIRST MATING OF HOLSTEIN FRIESIAN HEIFERS Budimulyati S., L.; Noor, R.R.; Saefuddin, A.; Talib, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 3 (2012): (September)
Publisher : Diponegoro University

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Abstract

The body weight records of 1221 heifers were used in this study collected from PT Taurus DairyFarm Sukabumi from year 2001 until 2011. The records that could be used for analysis were 373 out of1221 heifers, having completed data from birth to first mating period. Three different models i.e,Logistic, Gompertz, and von Bertalanffy were performed to analyze the growth rate of heifers. Theresults showed that the three models had different accuracy and heavily depend on age, environment andduration of recording. The body weights of sexual maturity and on certain ages were affected by theduration of recording. The Gompertz model was performed as the simpliest model in form ofcalculation. On the other hand, the Logistic was more difficult to calculate. All models indicated highaccuracy with the determination coefficient (R2) more than 90%. Based on the comparison, theGompertz and Logistic model are recommended for predicting the growth rate of heifers from birth tosexual maturity.
ANTIBODY POLYCLONAL PRODUCTION ON RABBIT ANTI-OVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (Rabbit anti-ovPAG) Setiatin, E.T.; Sajuthi, D.; Purwantara, B.; Talib, C.; Fathul, F.; Adiati, U.; Praira, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

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Abstract

The aim of the study was to produce polyclonal antibody (rabbit anti-ovPAG) which could detectPAG in the urine of pregnant ewes. Twelve rabbits were immunized against ovPG DEAE-TrisHCl (DT),DEAE-NaCl 20mM (DN2), DEAE-NaCl 40mM (DN4), DEAE-NaCl 80mM (DN8), DEAE-NaCl160mM (DN16), DEAE-NaCl 320mM (DN32) and DEAE-NaCl 1M (DN1) and NaCl 0.9 % as aplacebo. The 0.5 ml of isolate (purified from ovine cotyledon) was emulsified in equal volume withcomplete and incomplete Freud’s adjuvant. The mixture of each isolate and adjuvant was injected atmutiple sites along the dorsal area of rabbits by subcutaneous route. Blood were collected from marginalear vein, starting before first injection (baseline) and every 14 days. Rabbit anti-ovPAG were measuredusing Modified ELISA Technique. By using Western Blot Technique, DN32 showed the best immuneresponse among others and also could differenciate ovPAG in the urine of pregnant ewes It could beconcluded that ovPAG DN32 is a specific source of rabbit anti-ovPAG production. Protein of ovPAG atmolecular weight 31 kDa is a pregnancy protein marker of garut sheep and could be developed as amajor protein for producing antibodi.
Extraction and isolation of Ovine Pregnancy-Associated Glycoprotein (ovPAG) from cotyledon placenta of Garut sheep Setiatin, E.T.; Sajuthi, D.; Purwantara, B.; Talib, C.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

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Abstract

Pregnancy-associated glycoprotein (PAG) structurally related to aspartic protease, expressed in the outer epithelial cell layer (trophectoderm) of ungulate placenta. Ovine PAG (ovPAG) synthesized by mono- and binucleic trophoblast before complete implantation at Day 14-15. Of this, ovPAG could be used as a marker for early pregnancy. The objective of study was to extract and isolate PAG from placenta of Garut Sheep collected at term and to characterize their molecular weight. The procedures included extraction of protein at neutral pH (cotyledon was thawed, minced, added PBS, blended and centrifuged), acidic (H3PO41M, pH 4,5; centrifuged) and ammonium sulfate (40% and 80% (NH4)SO4, centrifuged) precipitation; gel filtration (Sephadex-G75), anion exchange chromatography (DEAE- cellulose). Cotyledon extract was subjected to Sephadex-G75 and DEAE cellulose, and their fractions were measured their absorbances. Absorbances of Sephadex-G75 and DEAE fractions at peak were assayed for protein concentration (Bichinconinic protein assay). Continuously, these fractions were subjected to monogel SDS-PAGE and stained by Commassie Brilliant Blue. It was four different molecular weights isolating from cotyledon of Garut Sheep, namely 68.8, 36.04, 32.39 and 12.18  kDa. However, after chromatography anion exchange (DEAE-cellulose), only  three bands exist, those were  71,67;  33,64 and 30,86 kDa. Key words: Garut sheep, Pregnancy-Associated Glycoprotein (PAG), Cotyledone, SDS-PAGE, DEAE-cellulose
Efektivitas Catatan Test Day untuk Evaluasi Genetik Produksi Susu pada Sapi Perah Indrijani, H.; Anang, A.; Noor, R. R.; Talib, C.
Zuriat Vol 14, No 1 (2003)
Publisher : Zuriat

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Abstract

Penelitian ini bertujuan untuk mempelajari efektivitas catatan Test day dengan model regresi tetap untuk evaluasi genetic produksi susu pada sapi perah berdasarkan laktasi satu dan dua. Data produksi susu berasal dari sapi perah Frisian Holstein periode laktasi satu dan dua dari tahun 1989–2000 di PT. Taurus Dairy Farm Sukabumi. Catatan Test day sebanyak 7503 buah berasal dari 456 ekor sapi betina, 40 pejantan dan 342 ekor induk. Model yang digunakan adalah Model Regresi Tetap, varian komponen diduga dengan Animal Model dengan Restricted Maximum Likelihood (REML) dan Nilai Pemuliaan dengan Best Linier Unbiased Prediction (BLUP). Hasil analisis menunjukkan hari test ke-35 (TD2) merupakan puncak produksi. Pengaruh musim terhadap produksi susu Test day mempunyai pola yang kurang jelas. Ragam genetik aditif pada laktasi dua menurun sejalan dengan penambahan catatan Test day sampai dengan Test day ke-3, setelah itu meningkat sampai akhir laktasi. Dugaan nilai heritabilitas mengikuti pola yang sama dengan ragam genetik. Evaluasi genetic berdasarkan laktasi satu penuh yang ditambah dengan satu catatan Test day pada laktasi dua sudah cukup untuk mengevaluasi seluruh ternak, sedangkan untuk mengevaluasi pejantan, evaluasi harus diperpanjang sampai 3 catatan Test day pada laktasi dua.
Extraction and isolation of Ovine Pregnancy-Associated Glycoprotein (ovPAG) from cotyledon placenta of Garut sheep Setiatin, E.T.; Sajuthi, D.; Purwantara, B.; Talib, C.
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Animal Sciences Society

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Abstract

Pregnancy-associated glycoprotein (PAG) structurally related to aspartic protease, expressed in the outer epithelial cell layer (trophectoderm) of ungulate placenta. Ovine PAG (ovPAG) synthesized by mono- and binucleic trophoblast before complete implantation at Day 14-15. Of this, ovPAG could be used as a marker for early pregnancy. The objective of study was to extract and isolate PAG from placenta of Garut Sheep collected at term and to characterize their molecular weight. The procedures included extraction of protein at neutral pH (cotyledon was thawed, minced, added PBS, blended and centrifuged), acidic (H3PO41M, pH 4,5; centrifuged) and ammonium sulfate (40% and 80% (NH4)SO4, centrifuged) precipitation; gel filtration (Sephadex-G75), anion exchange chromatography (DEAE- cellulose). Cotyledon extract was subjected to Sephadex-G75 and DEAE cellulose, and their fractions were measured their absorbances. Absorbances of Sephadex-G75 and DEAE fractions at peak were assayed for protein concentration (Bichinconinic protein assay). Continuously, these fractions were subjected to monogel SDS-PAGE and stained by Commassie Brilliant Blue. It was four different molecular weights isolating from cotyledon of Garut Sheep, namely 68.8, 36.04, 32.39 and 12.18  kDa. However, after chromatography anion exchange (DEAE-cellulose), only  three bands exist, those were  71,67;  33,64 and 30,86 kDa. Key words: Garut sheep, Pregnancy-Associated Glycoprotein (PAG), Cotyledone, SDS-PAGE, DEAE-cellulose