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Perbanyakan Padi Fl Interspesifik untuk Bahan Silang Balik (Back Cross) Syukur, M.; Aswidinnoor, H.; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 31, No 2 (2003): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

A lot of plant materials are needed for successful back cross program of interspecific FI rice to their recurrent parents. Multiplication of the steril FI plants through tiller propagation is not adequate. The research aims to develop technique of vegetatif multiplication of interspesific hybrid rice utilizing nodal segments. In the vegetatif multiplication experiment, several factors were examined as treatments: nutrition culture (MS, Yoshida, and water), stum position (first, second and third) and growth media (vermiculite, husk charcoal, and sand). The plant materials are interspecific FI rice i.e. Ranah Sanra (genom AA) x O. officinalis 100870 (genom CC), Hawara Bunar (genom AA) x O. Punctata 9101411 (genom BB), Grogol (genom AA) x O. punctata 9101411 (genom BB), CT 6510-24-1-3 (genom AA) x O. malamphuzaensis 100957 (genom BBCC). Results of the study indicated that MS and Yoshida nutrition, stum without sheath, base of stum and sand media gave better growth more than other treatments. Key words: Vegetatif multiplication, FI interspecific
Analisis Stabilitas Hasil Tujuh Populasi Jagung Manis Menggunakan Metode Additive Main Effect Multiplicative Interaction (AMMI) Sujiprihati, S.; Syukur, M.; Yunianti, R.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 34, No 2 (2006): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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The objective of this study was to identify the stability of seven sweet corn genotypes as breeding result of Center for Crop Improvement Studies (PSPT), using Additive Main Effect Multiplicative Interaction (AMMI) method. The design was Randomized Complete Block Design (RCBD) with three replications as blocks. The genotypes used were; PSPT-C, PSPT-K, PSPT-T1, PSPST-T2, PSPT-MM, and two commercial varieties Bogor-Hi and SD-2. The genotypes were planted in four different locations which included experiment fields of IPB-Cikabayan Darmaga (250 m above sea level) and Pasir Sarongge Cipanas (1120-1200 m above sea level), Gunung Geulis Cisarua (550 m above sea level), and Cibedug Ciawi (600 m above sea level). Based on the postdictive success and predictive success methods, the model used (AMMI 2) was able to explain interaction-influenced variation as much as 85%. The genotypes found stable in four locations were PSPT-MM, PSPT-T1, Bogor-Hi and SD-2. PSPT-C was specific for Ciawi, PSPT-K and PSPT-T2 specific for Cisarua.   Key words: AMMI, sweet corn, multilocation trials.
INDUKSI POLIPLOIDI MENGGUNAKAN KOLKISIN SECARA IN VIVO PADA BIBIT ANGGREK BULAN (Phalaenopsis amabilis (L.) BLUME) Rahayu, Eka Martha Della; Sukma, Dewi; Syukur, M.; Aziz, Sandra A.; Irawati, Irawati
Buletin Kebun Raya Vol 18, No 1 (2015): Buletin Kebun Raya Vol. 18 (1) January 2015
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Induksi poliploidi pada bibit Phalaenopsis amabilis telah dilakukan menggunakan kolkisin secara in vivo. Induksi poliploidi dilakukan dengan meneteskan kolkisin pada pucuk bibit P. amabilis.  Tujuan penelitian ini adalah untuk mendapatkan konsentrasi kolkisin yang efektif untuk induksi poliploidi bibit P. amabilis dan  menghasilkan bibit P. amabilis poliploid. Percobaan disusun dalam rancangan kelompok lengkap teracak dengan satu faktor, yaitu konsentrasi kolkisin. Pucuk bibit P. amabilis ditetesi 0,01 ml kolkisin (0, 1000, 2000, 3000, 4000, dan 5000 mg L-1). Hasil percobaan menunjukkan bahwa peningkatan konsentrasi kolkisin dari 1000 sampai 5000 mg L-1 tidak berpengaruh nyata terhadap persentase hidup dan pertumbuhan bibit pada 24 minggu setelah perlakuan (24 MSP). Bibit P. amabilis poliploid dapat dihasilkan pada penetesan kolkisin 1000, 3000, 4000, dan 5000 mg L-1 dengan konsentrasi kolkisin paling efektif adalah 5000 mg L-1. Bibit poliploid memiliki ukuran stomata lebih besar dari bibit diploid sebaliknya kerapatan stomatanya lebih rendah.
Pendugaan Parameter Genetik Vigor untuk Viabilitas dan Vigor Benih Cabai (Capsicum annuum L.) Menggunakan Analisis Setengah Dialel Ekowahyuni, Luluk Prihastuti; Syukur, M.; Sutjahjo, Surjono H.; Suhartanto, M. R.
Jurnal Hortikultura Indonesia Vol 6, No 3 (2015): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia dan Departemen Agronomi dan Hortiluktura

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ABSTRACTThe purpose of this study was to estimate the narrow sense heritability (h2ns), broad sense heritability (h2bs), heterosis, and heterobioltiosis, coefficient of genetic variances, coefficient of phenotypic variances, additive and dominant variances. Pepper population used in this study consisted of IPB C2, IPB C9, IPB C10, IPB C15 and half diallel hybrid. To estimate the effect of reciprocal IPB C10 x IPB C2 hybrid was used. Accelerated aging method was used to test the vigor using methanol 20% in five periods of time 0, 2, 4, 6 and 8 hours. Observations consisted of: (1) germination rate, (2) the length of radicle, (3) the length of hypocotile, (4) dry weight of normal seedlings, (5) speed of growth, (6) electrical conductivity and (7) moisture content. Genetic parameters were estimated using affinity analysis. Reciprocal effect indicated that there was no maternal effect. Parents with high combining ability was IPB C15 and that with high specific combining ability was IPB C2 x IPB C5 and gave positive heterosis.Key words: pepper, genetic coefficient, heterosis, seed vigor, specific combining ability ABSTRAKPenelitian ini bertujuan menduga nilai parameter genetik untuk viabilitas dan vigor benih cabai menggunakan metode persilangan setengah dialel untuk mendapatkan informasi nilaiheritabilitas, heterosis, koefisien keragaman genetik, varians aditif dan dominan, sehingga diharapkan dapat mendukung keberhasilan program pemuliaan yang mengkombinasikan karakter tanaman dengan viabilitas dan vigor benih yang baik. Empat genotipe tetua cabai yang digunakan adalah IPB C2, IPB C9, IPB C10, IPB C15, dan enam genotipe cabai hasil persilangan antar tetua adalah IPB C2 x IPB C9, IPB C2 x IPB C10, IPB C2 x IPB C15, IPB C9 x IPB C10, IPB C9 x IPB C15, IPB C10 x IPB C15. Rancangan percobaan yang digunakan adalah Rancangan Kelompok Lengkap Teracak (RKLT). Pengamatan untuk pengujian vigor dan viabilitas dilakukan pada genotipe tetua dan persilangan dengan tolok ukur yaitu (1) daya berkecambah (2) panjang akar, (3) panjang hipokotil, (4) bobot kering kecambah (5) kadar air benih, (6) kecepatan tumbuh (Kct), dan (7) daya hantar listrik (DHL). Pengujian vigor daya simpan benih cabai pada genotipe tetua dan persilangan menggunakan metode pengusangan cepat methanol 20%. Setelah pengujian vigor daya simpan benih dilakukan analisis varian dan analisis regressi dan analisis statistik biometrik. Hasil analisis menunjukkan bahwa genotipe cabai IPB C15 mempunyai nilai daya gabung umum yang tinggi untuk vigor daya simpan benih sehingga dapat dijadikan sebagai tetua untuk perakitan varietas F1 hibrida vigor daya simpan benih tinggi. Kombinasi persilangan tetua IPB C2 x IPB C15 menghasilkan benih yang memiliki vigor daya simpan benih tinggi.Kata kunci : cabai, daya gabung khusus, heterosis, koefisien genetik, vigor benih
EVALUASI KERAGAMAN GALUR MUTAN ARTEMISIA HASIL IRADIASI GAMMA Purnamaningsih, Ragapadmi; Lestari, E.G.; Syukur, M.
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 6, No 2 (2010): Desember 2010
Publisher : BATAN

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Serangan penyakit malaria di Indonesia terus meningkat. Di lain pihak, penyebab penyakit ini yaitu Plasmodium falciparum telah resisten terhadap obat malaria yang selama ini digunakan. Artemisinin, dari tanaman artemisia telah diteliti dapat mengendalikan malaria. Permasalahan yang dihadapi adalah kandungan artemisinin dari Artemisia annua yang ditanamdi Indonesia masih sangat rendah, yaitu berkisar 0.1 — 0.5 %. Peningkatan keragaman genetik artemisia dengan menggunakan iradiasi sinar gamma merupakan metode alternative untukmengatasi masalah tersebut. Pada penelitian sebelumnya telah dilakukan induksi mutasi pada biji artemisia dengan dosis 10-100 Gy. Planlet hasil iradiasi yang mempunyai perakaran yang baik, diaklimatisasi di rumah kaca dan galur mutan yang didapatkan ditanam di Kebun Percobaan Gunung Putri, Balittro dengan ketinggian 1545 m dpl. Sebagai pembanding digunakan tanaman yang berasal dari biji (kontrol biji) dan tanaman dari kultur in vitro yang tidak diradiasi (kontrol in vitro). Hasil penelitian menunjukkan bahwa galur-galur mutan nmemperlihatkan keragaman morfologi, antara lain tinggi tanaman, bentuk daun, umur berbunga. Galur mutan umumnya berbunga lebih lambat dibandingkan tanaman kontrol. Sepuluh galur mutan telah terseleksi berdasarkan bobot basah dan bobot kering tanaman yang kemudian di analisis kandungan artemisininnya. Hasil penelitian menunjukkan kandungan artemisinin dari galur mutan bervariasi antara 0.44 — 1.41%, sedangkan kandungan artemisinin dari tanaman kontrol in vitro adalah 0.43%.
Analisis Stabilitas Hasil Tujuh Populasi Jagung Manis Menggunakan Metode Additive Main Effect Multiplicative Interaction (AMMI) Sujiprihati, S.; Syukur, M.; Yunianti, R.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 34, No 2 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

The objective of this study was to identify the stability of seven sweet corn genotypes as breeding result of Center for Crop Improvement Studies (PSPT), using Additive Main Effect Multiplicative Interaction (AMMI) method. The design was Randomized Complete Block Design (RCBD) with three replications as blocks. The genotypes used were; PSPT-C, PSPT-K, PSPT-T1, PSPST-T2, PSPT-MM, and two commercial varieties Bogor-Hi and SD-2. The genotypes were planted in four different locations which included experiment fields of IPB-Cikabayan Darmaga (250 m above sea level) and Pasir Sarongge Cipanas (1120-1200 m above sea level), Gunung Geulis Cisarua (550 m above sea level), and Cibedug Ciawi (600 m above sea level). Based on the postdictive success and predictive success methods, the model used (AMMI 2) was able to explain interaction-influenced variation as much as 85%. The genotypes found stable in four locations were PSPT-MM, PSPT-T1, Bogor-Hi and SD-2. PSPT-C was specific for Ciawi, PSPT-K and PSPT-T2 specific for Cisarua. ? Key words: AMMI, sweet corn, multilocation trials.
Perbanyakan Padi Fl Interspesifik untuk Bahan Silang Balik (Back Cross) Syukur, M.; Aswidinnoor, H.; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 31, No 2 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

A lot of plant materials are needed for successful back cross program of interspecific FI rice to their recurrent parents. Multiplication of the steril FI plants through tiller propagation is not adequate. The research aims to develop technique of vegetatif multiplication of interspesific hybrid rice utilizing nodal segments. In the vegetatif multiplication experiment, several factors were examined as treatments: nutrition culture (MS, Yoshida, and water), stum position (first, second and third) and growth media (vermiculite, husk charcoal, and sand). The plant materials are interspecific FI rice i.e. Ranah Sanra (genom AA) x O. officinalis 100870 (genom CC), Hawara Bunar (genom AA) x O. Punctata 9101411 (genom BB), Grogol (genom AA) x O. punctata 9101411 (genom BB), CT 6510-24-1-3 (genom AA) x O. malamphuzaensis 100957 (genom BBCC). Results of the study indicated that MS and Yoshida nutrition, stum without sheath, base of stum and sand media gave better growth more than other treatments. Key words: Vegetatif multiplication, FI interspecific