Articles

Imunisasi Ikan Jambal Siam dengan Vaksin Ichthyophthirius multifiliis Syawal, Henni; Ikhwan Siregar, Yusni
Jurnal Veteriner Vol 11, No 3 (2010)
Publisher : Jurnal Veteriner

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Abstract

An experiment on the immunization of Jambal Siam ( Pangasius hypophthalmus) withIchthyophthirius multifiliis vaccine was conducted in the Laboratorium of Parasitic and Fish Diseases,Faculty Fisheries and Marine Science of Riau University, and Laboratorium of Fish Health of FacultyFisheries and Marine Science, IPB. The objective of the study was to enhance immune system of the fishfry on the ichthyophthiriasis diseases. The vaccine was prepared by prolonged treatment of theron in waterbath of 47°C for 30 minutes. The vaccine was administrated to fish by immersion in aquaria. A completelyrandomized design (CRD) in factorial pattern (3 X 3 X 3) was carried out with dose and time as factors.Doses of treatment and time of immersion were of 1 ml/L, 2 ml/L and 3 ml/L as well as 15, 30 and 45minutes of treatment time respectively. To evaluate the effectiveness of vaccine in fish, a challenge test tofish was done at day 15 with theron 90.000 cell/ aquaria until rearing day 25 after vaccination. It revealedthat the best performance (p<0.5) were of dose 3 ml/L and time treatment of 15 minutes in that survivalrate of fish was 100% followed by dose 3 ml/L with time 45 minutes (63,3%). The total erythrocyte count,hematocrit level and hemoglobin of tested fish were fluctuated. The water quality were recorded including;dissolved oxygen range from 3,74 - 4,98 ppm; temperature 25 - 30°C and acidity of 4 - 6, quality were ofnormal range for fish. The optimal vaccine dose is 3 ml/l with 15 minute immersion.
RESPON FISIOLOGIS IKAN JAMBAL SIAM (Pangasius hypopthalamus) PADA SUHU PEMELIHARAAN YANG BERBEDA Syawal, Henni; S, Yusni Ikhwan
Jurnal Terubuk Vol 39, No 01 (2011)
Publisher : Jurnal Terubuk

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Fish stress may be governed by change of water temperature that increaseboth chatecolamine and cortisal secretion. In high level both hormone have anegative effects on fish immune system. Increasing of cortical in blood plasmawould lead to inhibition of interleukin I and II formation. Thus, fish immunesystem dropped which cause fish suffer from infection and eventually dead.Physiological stress may stimulate increasing of cortical and glucose level ofblood plasma. As such lysozime activity would increase. Increase of bloodglucose is of a normal indicator of stressed fish. An randomized block designexperiment with one factor, have been carried out to evaluate the effect or watertemperature on blood glucose of fish. Three level of water temperature ( 24, 28.and 280C) were applied. It revealed that blood glucose level ranged from 127.23to 192,96gr/dl with maximum level of glucose appeared in fish reared in 240C (12gr/dl), and the lowest at of 32oC.. Blood hemoglobin in accordance with bloodglucose, in which maximum level found in 240C (12 gr/dl). The highesthematocrite level were appeared in treatment of 240C (39,83%). Red BloodNumber of 4.725 X 103 sel/mm3, were found in the water temperature of 240C.
Histology of Liver and Kidney of Mystus nemurus that immersed with Curcuma xanthorrhiza, ROXB extract Lubis, Fauzi Ahmad; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was carried out in November 2013 until January 2014 in the laboratory of Fish Diseases and Parasites, Faculty of Fisheries and Marine Science Riau University Pekanbaru. The purpose of this research was to understand the histological structure of liver and kidney of Mystus nemurus that is immersed with Curcuma xanthorrhiza ROXB extract. The method used in this study was experimental and the histologycal structure of the fish will be analyzed descriptively. The treated fish were immerse in 3 different concentration of Curcuma xanthorrhiza ROXB extract, they were (P1 (0.2 g/L), P2 (0.4 g/L, P3 (0.6 g/L). for five minutes/ day for 30 days period. The negative control were fishes that were not treated with Curcuma xanthorrhiza ROXB extract. The liver showed hemorhage, fatty degenerative and necrosis. The kidney showed necrosis and hemorhage. It was concluded that immersion of fish in Curcuma xanthorrhiza ROXB extract shown histologycal alterations in the tissue of Mystus nemurus.Key words: Mystus nemurus, Curcuma xanthorrhiza ROXB, kidney, liver
Respons Of Erythrocytes, Hematocrit And Hemoglobin River Catfish (Mystus Nemurus) Combination Fed Guava Leaves (Psidium Guajava) And Bitter (Andrographis Paniculata Ness) Susanti, Mesi; Lukystiowati, Iesje; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This study was conducted from April-June 2013 in the Laboratory of Aquaculture and Fish Diseases and Parasites Laboratory Faculty of Fisheries and Marine Sciences University of Riau. The purpose of this study was to determine the response of erythrocytes, hematocrit, and blood hemoglobin river catfish (Mystus nemurus) fed a combination of guava leaves and bitter infected with the bacterium A. hydrophila. This study used 5 treatments of: negative control (without any solution guava, bitter and non-infected A. hydrophila fish meal), Kp: positive control (without guava leaves and a bitter and infected A. hydrophila fish meal), P1 (addition of guava leaves as much 10 g and 10 g bitter / kg of feed fish meal), P2 (addition of guava leaves and bitter as much as 20 g of 20 g / kg of feed fish meal), P3 (addition of guava leaves and bitter as much as 30 g of 30 g / kg of feed fish meal). The results showed that feeding a combination of guava leaves and bitter can not increase erythrocytes, hematocrit, and hemoglobin but it can increase endurance fish seen from passing fish alive after are infected A. hydrophila. Average erythrocytes from 0.52 to 2.90 million cells/mm3, 16.60 to 50.97% hematocrit and hemoglobin percentage from 3.62 to 5.33 g /%Key word: River catfish (Mystus nemurus), guava leaves and bitter, A. hydrophila
SENSITIVITAS EKSTRAK BUAH MENGKUDU (Morinda citrifolia L.) TERHADAP BAKTERI Streptococcus agalactiae Muharram, Anzila Rizki W; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThe research was conducted from October to December 2014 in the Laboratory of Parasitic Diseases of Fish Faculty of Fisheries and Marine Sciences, and Laboratory of organic and natural material of Faculty of Mathematics and Natural Sciences, University of Riau, Pekanbaru. The purpose of this study to determine the concentration of noni fruit (Morinda citrifolia L.) that can inhibit the growth of Streptococcus agalactiae and safe dose to be given to the fish. The method that had been used was experimental method with concentration : P0: control, P1 (4%), P2 (5%), P3 (6%), P4 (7%), P5 (8%), P6 (9%), P7 (10%). The results show noni fruit solution to Streptococcus agalactiae sensitive average highest inhibition zone formed on treatment P7 (10%) of 9.83 mm and significantly different between treatments (P <0.05). MIC test of extract methanol Morinda citrifolia L. on treatment P2 (5%) of 78 x 108 cfu/mL. LD50 test solution for tilapia (Oreochromis niloticus) extract methanol Morinda citrifolia L. marinated for 24 hours after Reed and Muench analysis contained in the concentration of 1.42%.Key word: Morinda citrifolia L., Streptococcus agalactiae, Sensitivitas, MIC, LD50.
IDENTIFICATION OF PATHOGENIC BACTERIA OF TILAPIA (Oreochromis niloticus) IN SUBDISTRICT MARPOYAN DAMAI PEKANBARU Lubis, Dicky Azwar; Syawal, Henni; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis research was done in April - Juni 2014 in Fish Disease Parasite and Laboratories and an Experiment of the Faculty of Fisheries and Marine Science University of Riau. This study aims to determine the types of pathogens that attack tilapia in District Marpoyan Damai Pekanbaru. The benefits of this research is to provide information to farmers about the types of pathogens in tilapia, so it can be the prevention of disease. The method used in this study is a survey method by taking samples in the field and analyzed in the laboratory. The amount of bacteria found from both the ponds was six species. Bacteria found in a pond one these were Aeromonas hydrophila, Basillus sp, Pseudomonas sp and Streptococcus sp. Meanwhile bacteria found in a pond two these were Basillus sp, Stapyhilococcus sp, and Edwardsiella tarda.Keywords: Pathogenic Bacteria, Tilapia,
Sensitivity of Mangosteen Rind (Garcinia mangostana L.) Solution Toward Aeromonas hydrophila M, Dian Fitria; Lukistyowati, Iesje; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTResearch was conducted from September to October 2014, at the labory Examination of Fish Disease, Fisheries and Marine Sciences Faculty of Riau University. The Purpose of this study was to determine the sensitivity of mangosteen rind (G. mangostana L.) as antimicrobial to A. hydrophila, the range of Minimal Inhibitory Concentration (MIC) and the lethal dose (LD50) mangosteen rind (G. mangostana L.) solution against to catfish (Clarias gariepinus) (10-13 cm) by immersion. The research method used doses 10 % (19 g/L), 20 % (38 g/L), 30 % (57 g/L), 40 % (76 g/L), 50 % (95 g/L), 60 % (114 g/L), 70 % (133 g/L), 80 % (152 g/L), 90 % (171g/L), 100 % (190 g/L). The results showed that mangosteen rind was effectively to inhibit the growth of bacterial A. hydrophila at a dose of 50 % (95 g/L) inhibitory zone of 13,65 mm, with the number of A. hydrophila colonies in TSA medium dose 50 % (95 g/L) is 30,6 x 108 cfu/mL. LD50 test was obtained in the dose mangosteen rind 4, 43 g/L.Key words : Garcinia mangostana L., Aeromonas hydrophila, antimicrobial, lethal dose
LEUKOCYTES DIFFERENTIATION OF Pangasius hypopthalmus THAT WERE FEED WITH CURCUMIN EXTRACT FROM Curcuma domestica V Iman, Khasan Nur; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted on June until August 2016 in Parasites and Fish Diseases Laboratory, Faculty of Fisheries and Marine science, University of Riau, Pekanbaru. The aim of this research was to determine leucocyte differentiation of Pangasius hypopthalmus that were feed with curcumin extract from Curcuma domestica V. This research used five treatment such as; Kn (negative control): that were feed without curcumin extract and were not infected with A. hydrophila, Kp (positive control): that were feed without curcumin extract and infected with A. hydrophila, P1: feed with curcumin extract in 0,3g/kg, P2: feed with curcumin extract in 0,5g/kg and P3: feed with curcumin extract in 0,7 g/kg. Fish reared in an aquarium the size of 40x30x30 cm with stocking density 10 fish / aquarium, feeding as many as 10% of body weight and do 3 times a day. Pisciculture carried out for 30 days and after that fish infected with Aeromonas hydrophila. The results shown that P3 (feed with curcumin extract in 0,7 g/kg) were the best treatment with lymphocytes 83,00%; monocytes 5.33%; neutrophils 3,67%; thrombocyte 8.00%; total leukocytes 12,01x104 cell/mm3; and fagocyte index 58,33.Keyword: Leukocytes, Pangasius hypopthalmus, Curcuma domestica V, Curcumin Extract
HEMATOLOGYCAL CONDITION OF TILAPIA (Oreochromis niloticus) THAT WERE INFECTED BY ECTOPARASITES IN PONDS IN SIMPANG TIGA SUB-DISTRICT PEKANBARU Rizki, Isnaini Lidya; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis research was conducted in July to September 2014 in Parasite and Fish Diseases Laboratory of Fisheries and Marine Science Faculty, Riau University. The aim of this research to understand the hematologycal condition of tilapia (Oreochromis niloticus) that were infected by ectoparasites. The method used in this study is a survey method by taking fish samples from one location and the fish sample will be taken in the laboratory and identified. Ectoparasite examinations will be done based on Kabata, 1985 and hematologycal parameter will be measured based on Siwicki and Anderson, 1993. Result shown that the three types of ectoparasites that were infected in tilapia are: Trichodina sp., Dactylogyrus sp., and Gyrodactylus sp. The average of hematocrit value of male tilapia was 19,7- 22,18%, the hemoglobin concentration was 4.07-4.8%, and the total erythrocyte was 1.483.000-1.680.000 cells / mm3, whereas the hematocrit of female tilapia was 18,9-21,6%, the hemoglobin concentration was 3,8-4,9%, the total erythrocytes was 1.346.000-1.562.500 cells / mm3. It can be concluded that the hematologycal conditions of tilapia (male and female) in that area was different.Keywords : Ectoparasites, Hematologycal, Tilapia, Pekanbaru
LEUKOCYTES DIFFERENTIATION PROFIL OF TILAPIA (Oreochromis niloticus) THAT WERE INFECTED BY ECTOPARASITES IN POND IN SIMPANG TIGA SUB-DISTRICT PEKANBARU Syawal, Henni; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis research was conducted in Oktober to Desember 2015 in the Laboratory of Parasite and Fish Disease of Fisheries and Marine Science Faculty, University of Riau. This study aims to understand the leukocytes differentiation Profil of tilapia (Oreochromis niloticus) that were infected by ectoparasites in Simpang Tiga sub-district Pekanbaru. The research method used in this study is a survey method by taking sampels in the field and fish sample will be analized in the laboratory, and will be identificied based on Kabata, 1995. The hematologycal parameter (leukocytes differentiation) will be measured based on Swicki and Anderson, 1974. The result shown that three types of ectoparasite that were infected in tilapia was Trichodina sp., Dactylogyrus sp., and Gyrodactylus sp. The total leukocytes of male tilapia was 92.350-103.400 cells/mm3 and famale tilapia was 89.375-100.200 cells/mm3. Differentiation of leukocytes such as lymphocytes of male tilapia was 65-73,5%, whereas the famale tilapia was 61,5-77,5%, monocytes of male tilapia was 22-31,5%, whereas the famale tilapia was 18-34,5%, and neutrophils of male tilapia (3-4,5%) was higher than famale tilapia (4-5%).Keywords : Ectoparasites, Differentiation, Leukocytes, Tilapia, Pekanbaru