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The changes of viability, vigor, and biochemical content of Trema (Trema orientalis Linn. Blume) seeds during storage Yuniarti, Naning -; Syamsuwida, Dida; Kurniaty, Rina
Jurnal Penelitian Kehutanan Wallacea Vol 7, No 1 (2018): Jurnal Penelitian Kehutanan Wallacea
Publisher : Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.585 KB) | DOI: 10.18330/jwallacea.2018.vol7iss1pp83-92

Abstract

The seed of trema (Trema orientalis Linn. Blume) is categorized as physiologically intermediate which decline faster during storage compared to orthodox seeds, resulting in the changes of its viability, vigor and biochemical contents.The study was objected to determine the changes of viability, vigor and biochemical contents of trema seeds during storage. The seeds were collected from Badung - Bali province. Seeds testing were carried out in a laboratory and glasshouse of Forest Tree Seed Technology Research & Development Centre. Biochemical analysis was implemented in Soil and Plant Laboratory, Seameo- Biotrop.  A Completely Randomized Design was employed in this trial with a treatment of storage periods (0.1, 2, 3, 4, 5 and 6 months). The parameters were germination capacity, germination speed, moisture content and biochemical content including carbohydrate, protein and fat. The results revealed that all the parameters were significantly affected by storage periods. After six months periods of trema seeds storage, it would caused the decrease of moisture content (6.2%), germination capacity (61%), germination speed (2.5%/etmal), carbohydrate (19.2%) and protein contents (1.8%). However, the content of fat was increased significantly (10.2%).
KAJIAN PENGGUNAAN PACLOBUTRAZOL TERHADAP PERTUMBUHAN SEMAI AGATHIS LORANTHIFOLIA (THE STUDY OF PACLOBUTRAZOL ON THE GROWTH OF AGATHIS LORANTHIFOLIA SEEDLINGS) Satjapradja, Ombo; Setyaningsih, L; Syamsuwida, Dida; Rahmat, Ateng
Jurnal Manajemen Hutan Tropika Vol. 12 No. 1 (2006)
Publisher : Institut Pertanian Bogor (IPB University)

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Abstract

Paclobutrazol is one of hormone which able to inhibit the growth of tree seedling. This kind of hormone is usually used extensively in horticulture in stimulating flowering and enhancing fruiting. The objective of the research was to know the effect of paclobutrazol on the growth of Agathis loranthifolia seedlings during storage under different condition, in order to support seedling supply programs for forest establishment and rehabilitation. There were two factors of the trial i.e.) growth inhibitors (paclobutrazol, NaCl and aquadest) and 2) storage conditions (growth house, heavy shading and light shading). A complete randomized design with two factors and 3replication were used in this trial. The result of the research showed that the treatment of paclobutrazol to the seedlings stored at a green house gave an effect to the height and diameter growth of Agathis loranthifolia seedlings which were lower than those treated by NaCl and aquades. While paclobutrazol treated seedlings stored under light shading, gave higher value of seedling height and diameter than those stored under green house condition and heavy shading. The highest value of seedling survival Agathis loranthifolia achieved by paclobutrazol treated seedlings which were stored at the green house and under light shading. Field testing after 3 months plantation showed that paclobutrazol ? treated seedlings and seedlings, stored under light shading gave a highest value of seedling survival (100%) and seedling quality index (0,095).Keywords : paclobutarazol hormone, seedling storage technique, recalcitrant seed, seedling survival,  seedling quality index.
METODE PERLAKUAN PENDAHULUAN UNTUK PENYIMPANAN BENIH MELUR PADA SUHU SANGAT RENDAH (PRETREATMENT METHOD OF MELUR SEEDS STORING AT EXTREME LOW TEMPERATURE) Syamsuwida, Dida; Aminah, Aam
Jurnal Manajemen Hutan Tropika Vol. 14 No. 2 (2008)
Publisher : Institut Pertanian Bogor (IPB University)

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Abstract

Cryopreservation is a method of long term storage in which an extremely low temperature of nitrogen liquid (-196 oC) being used to preserve seeds of recalcitrant characteristics. The study was aimed to determine the standard protocol of vitrivication in melur (Podocarpus neriifolius) seeds storing in various cryoprotectans. The use of sucrose PVS2 and DMSO as a cryoprotectan were employed to protect the regularly-desiccated seeds during preserving in liquid nitrogen for 1 hour. Evaluation was made on the viability by germinating the seeds in green house. Randomized completely design of 3 factors was used in this study. The results revealed that seed moisture content was 53% after being desiccated using vaccuum-desicator for 24 hours and showed more than 85% of germination, in average. Vitrivicated seeds in DMSO for 2 hours performed 66.67% germination. Meanwhile, seeds in sucrose and PVS2 for 1 hour reached of 53.33% and 25.33% of germination, respectively. Preservation in liquid nitrogen for 1 hour did not succeed to keep any treated seeds as there was no germination found during viability testing.Keywords: cryiopreservation, vitrivication, melur, desiccation, recalcitrant
ECOLOGICAL CONDITIONS AND DISTRIBUTION OF GEMOR TREE SPECIES IN CENTRAL AND EAST KALIMANTAN Adinugroho, Wahyu C.; Sidiyasa, Kade; Rostiwati, Tati; Syamsuwida, Dida
Indonesian Journal of Forestry Research Vol 8, No 1 (2011): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

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Abstract

The aim of this study was to determine the ecological conditions and distribution of gemor bark producing tree species at Tuanan village in Kapuas District, Central Kalimantan Province and Long Daliq village in Kutai Barat, East Kalimantan Province. In order to collect adequate vegetation data, several obser vation plots were laid out by using purposive sampling. Primary and secondary data were collected from the plot areas by obser ving directly the habitat and its ecological condition of vegetation. It was revealed that the gemor tree species tended to grow well on the habitats which have a thin layer of peat (< 2 m), pH 3 – 4 and in a humid climatic condition.  Two gemor bark producing tree species were identidfied in the study areas, namely Nothaphoebe coriacea (Kosterm.) Kosterm. and  N. umbelliflora Blume. The similarity level of vegetation composition at both sites (Kapuas and Kutai Barat Districts) was low. The tree species richness in the plot areas of Tuanan in Kapuas District, Central Kalimantan (82 species, 57 genera and 28 families) was higher than that found in Long Daliq, Kutai Barat District, East Kalimantan (38 species, 26 genera and 19 families).
TEKNIK PENYIMPANAN SEMAI KAYU BAWANG ( Dysoxylum moliscimum ) MELALUI PEMBERIAN ZAT PENGHAMBAT TUMBUH DAN PENGATURAN NAUNGAN Syamsuwida, Dida; Aminah, Aam
Jurnal Penelitian Hutan Tanaman Vol 8, No 3 (2011): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Peningkatan Produktivitas Hutan

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Abstract

PENENTUAN KARAKTERISTIK FISIOLOGIS BENIH KRANJI ( BERDASARKAN NILAI KADAR AIR Pongamia pinnata) Aminah, Aam; Syamsuwida, Dida
Jurnal Penelitian Hutan Tanaman Vol 10, No 1 (2013): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Peningkatan Produktivitas Hutan

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Abstract

ABSTRACTPongamia pinnata is one of sources for biodiesel. This study aims to determine physiological and biochemical parameters of seed for planting and biodiesel production. Seed properties were analyzed such as moisture content, germination percentage, biochemical content (protein, starch and electrical conductivity) as well as oil contents. Initial moisture content of seeds was done by means of heat treatment in oven for 24 hours 103OC. Reduction of moisture content of seeds for germination test was conducted by means of incubation for 0, 24, 48 and 72 hours at 35OC. The results revealed the highest germination (92%) was reached at the moisture content of 46.80 % after desiccating for 48 hours. The content of protein and starch were 14.84% and 15.01% respectively and electrical conductivity was 815 millimho. Moisture content for seedling cultivation was 46.8% and for biodiesel was 51.37% with rendemen of 11.47%. The content of fat tended to decrease during desiccation.ABSTRAKKranji (Pongamia pinnata) adalah salah satu jenis tanaman yang berperan dalam menyediakan dua sumber energi yaitu biomassa kayu untuk kayu bakar dan biji mengandung minyak nabati yang dapat dimanfaatkan sebagai sumber biodiesel. Penelitian ini bertujuan untuk mengetahui parameter fisiologis dan biokimia setelah terjadinya penurunan kadar air dalam rangka penyediaan bahan tanaman dan bahan baku biodiesel. Metode yang digunakan untuk pengujian kadar air benih awal adalah pengeringan dengan oven pada suhu 1030C ± 20C selama 24 jam. Perlakuan penurunan kadar air benih dilakukan dengan metode penyimpanan dalam inkubator untuk P1 = 0 jam (kontrol); P2 =24 jam; P3 = 48 jam dan P4 = 72 jam. Parameter yang diamati meliputi kadar air, daya kecambah, kandungan biokimia benih (protein, pati dan Daya Hantar Listrik/DHL) serta rendemen minyak. Hasil penelitian menunjukkan bahwa pengeringan yang tepat untuk bibit kranji adalah 48 jam. Perlakuan ini menghasilkan benih dengan kadar air 46,39%, protein 14,84%, pati 15,01% dan DHL 815 millimho dan daya kecambah 92%. Perubahan biokimia benih kranji selama penurunan kadar air memperlihatkan adanya peningkatan protein dan daya hantar listrik seiring dengan lamanya pengeringan serta penurunan kandungan lemak dan kandungan pati. Berdasarkan perubahan fisiologis dan biokimia yang terjadi, benih kranji memiliki kecenderungan bersifat rekalsitran. Kadar air benih untuk bibit tanaman adalah 48,6% sedangkan untuk produksi biodiesel 51,37% dengan rendemen 11,47%. Kandungan minyak terus menurun selama penurunan kadar air.
DAMPAK PERUBAHAN FISIOLOGI DAN BIOKIMIA BENIH EBONI ( Diospyros celebica Bakh.) SELAMA PENYIMPANAN Yuniarti, Naning; Syamsuwida, Dida; Aminah, Aam
Jurnal Penelitian Hutan Tanaman Vol 10, No 2 (2013): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Peningkatan Produktivitas Hutan

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Abstract

ABSTRACTThe research was objected to determine the impact on biochemical and physiological changes of eboni (Diospyros celebica Bakh.) seeds during storage. Completely randomized design was used in this trial with two treatments, i.e storage room condition and storage periods. The results showed that physiological changes of the seeds during storage decreased their germination percentage and seed moisture content. Biochemical changes as indicated by the increase of fat and protein contents, as well as reduction of carbohydrat content following the period of drying and storage were observed. Storing the seeds in an Air-Conditioned room could keep germination percentages at 61,94% with the moisture content at 46,69% for as long as 8 weeks. Based on physiological and biochemical responses, eboni seeds could be classified as recalcitrant type.ABSTRAKEboni  (Diospyros  celebica Bakh.)  merupakan  jenis  tanaman  yang potensial  untuk dikembangkan  padapembangunan hutan tanaman. Benih bermutu tinggi diperlukan dalam program pengembangan jenis ini dan penyimpanan benih tidak dapat dihindari berkaitan dengan penyediaan benih. Penyimpanan benih eboni ditujukan untuk mempertahankan viabilitas benih agar tetap tinggi. Tujuan penelitian adalah untuk mengetahui dampak perubahan kandungan biokimia dan fisiologis benih eboni selama penyimpanan. Rancangan percobaan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap pola Faktorial dengan perlakuan faktor ruang simpan  dan  periode penyimpanan.  Hasil  penelitian  menunjukkan perubahan  fisiologis benih eboni  selama penyimpanan mengakibatkan adanya penurunan nilai daya berkecambah dan kadar air benih ; perubahan biokimia benih eboni selama penyimpanan menunjukkan adanya peningkatan kandungan lemak dan protein serta penurunan kandungan karbohidrat seiring dengan lamanya pengeringan dan penyimpanan ; penyimpanan benih eboni di ruang simpan AC dapat mempertahankan viabilitas benih selama 8 minggu, dengan daya berkecambahnya hingga 61,94% dengan kadar air 46,69 %. Berdasarkan reaksi fisiologis dan biokimia yang terjadi, maka benih eboni dapat dikatagorikan sebagai benih rekalsitran.
SIKLUS PERKEMBANGAN PEMBUNGAAN DAN PEMBUAHAN SERTA PEMBENTUKAN BUAH KEMENYAN (Styrax benzoin) DI AEK NAULI Syamsuwida, Dida; Aminah, Aam; Nurochman, Nurkim; Sumarni, E. Baeni; Ginting, Johan
Jurnal Penelitian Hutan Tanaman Vol 11, No 2 (2014): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpht.2014.11.2.89-98

Abstract

Jaminan ketersediaan benih secara terus menerus memerlukan informasi tentang fenologi pembungaan dan pembuahan. Informasi ini sangat bermanfaat untuk menduga waktu pemanenan yang tepat dan memperkirakan potensi produksi benih. Jumlah maksimum produksi benih yang dihasilkan dapat dideterminasi dari ratio bunga menjadi buah. Tujuan penelitian adalah mengetahui siklus reproduksi dan pembentukan buah jenis kemenyan (Styrax benzoin) di Aek Nauli. Pohon sampling dipilih sebanyak10 pohon dengan dua perlakuan yaitu pembungaan pada arah Timur dan Barat. Jumlah bunga dan buah per malai dihitung untuk mengukur ratio bunga menjadi buah (fruit set) tanaman kemenyan. Hasil pengamatan menunjukkan bahwa siklus reproduksi tanaman kemenyan di arboretum Aek Nauli pada periode tahun 2012–2013 berlangsung selama 8–9 bulan diawali dari terbentuknya tunas generatif pada bulan Juni–Juli, bunga mekar Agustus dan buah masak pada bulan Februari–Maret. Ratio buah/bunga (fruit set) kemenyan berkisar antara 10–13%. Pembungaan pada dahan bagian Barat dan Timur tidak menunjukkan perbedaan nyata. 
TEKNIK PENYIMPANAN SEMAI KAYU BAWANG ( Dysoxylum moliscimum ) MELALUI PEMBERIAN ZAT PENGHAMBAT TUMBUH DAN PENGATURAN NAUNGAN Syamsuwida, Dida; Aminah, Aam
Jurnal Penelitian Hutan Tanaman Vol 8, No 3 (2011): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpht.2011.8.3.147-153

Abstract

Kayu bawang (Dysoxylum moliscimum) adalah salah satu jenis pohon hutan yang dikenal memiliki benih dengan viabilitas yang cepat menurun dalam beberapa hari, sehingga penyimpanan benih untuk jenis ini masih menjadi kendala. Dengan demikian, perlu dilakukan penelitian penyimpanan semai dengan Dysoxylum moliscimum) menggunakan metode pertumbuhan lambat (slow growth). Tujuan penelitian adalah mengetahui pengaruh beberapa bahan pengatur tumbuh, kondisi simpan dan umur semai pada saat penyemprotan terhadap pertumbuhan semai jenis kayu bawang selama penyimpanan. Bahan pengatur tumbuh yang digunakan adalah paklobutrazol, NaCl dan akuades sebagai kontrol. Kondisi tempat simpan terdiri dari naungan berat, naungan sedang dan naungan ringan. Sedangkan umur semai pada saat penyemprotan adalah 1, 2 dan 3 bulan  Rancangan percobaan yang digunakan adalah rancangan acak lengkap pola faktorial. Hasil penelitian menunjukkan bahwa faktor yang menghambat pertumbuhan semai secara efektif sehubungan dengan upaya penyimpanan selama 6 bulan adalah penyemprotan semai umur 3 bulan dibawah kondisi naungan berat (T 25 0C, RH96 %, intensitas cahaya 650 lux) dan penerapan larutan NaCl 0,5%. Kondisi ini dapat menekan pertumbuhan tinggi dan diameter rata-rata sebesar 59,13 % serta memberikan persen hidup sebesar 95%.
THE CHANGE OF FAT, PROTEIN, STARCH CONTENS AND ELECTRIC CONDUCTIVITY WITHIN GAHARU (AQUILLARIA MALACCENSIS) SEEDS Syamsuwida, Dida; Aminah, Aam
Jurnal Manajemen Hutan Tropika Vol. 13 No. 2 (2007)
Publisher : Institut Pertanian Bogor (IPB University)

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Abstract

One of the symptomps of seed ageing is broad changes of biochemical content within seeds which is causing the decrease of decrease viability, eventually. The aim of the study was to determine the change of Aquillaria malaccensis seeds biochemically during storage and desiccating. There were two trial in this study: 1) decreasing seed moisture content by desiccations for 6, 12, 18, 24, and 48 hours and 2) storing the seeds conventionally in two different condtions of storage (ambient room and air conditioner room). Since the moisture content of fresh seed on acceptance was low (15,9%) due to the delaying during transportation, reducing moisture content of the seed brought about the decrease of germination capacity down to zero. The content of protein, fat and starch of the seeds during desiccation and storage were mostly  changes which were indicating the deterioration of the seeds. The content of fat of fresh seeds was high (46,2%) and tended to remain high during desiccating. Meanwhile, the content of protein tended to increase and starch was decreased following desiccation. The content of fat and protein tended to increase and starch was decreased following desiccation. the content of fat and protein during storage weree reduced, while starch was increased for 8 weeks and then went down for the rest of storage. The electricconductivity within the seeds in both treatments (desiccation and storage) was increased which were indicating the leakage of membrane cells occured . Based on biochemical content changes, seed of Aquillaria malaccensiscould be categorized as recalcitrant type. However, to make the results more accurate it should be proved specifically by testing the physiological of seeds, simultaneously.Keywords : Aquillaria malaccensis, desiccations, fat, moisture content, protein