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Journal : Jurnal Teknologi Pertanian

The Fermentation Study on Polyhydroxyalkanoates Produced by Ralstonia eutropha from Hydrolized Sago Starch as The Carbon Source Atifah, Nur; Syamsu, Khaswar; Suryani, Ani
Jurnal Teknologi Pertanian Vol 8, No 3 (2007)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Polyhydroxyalkanoates (PHAs), microbial bioplastics, not only were similar in properties with those made of petrochemical products, they were also completely biodegradable. At the moment, the price of PHAs was still high. This research was conducted to investigate the ability of Ralstonia eutropha to produce PHAs on a sago starch-based substrate, one of the cheap and abundantly renewable resources in Indonesia, and to characterize the PHAs produced. The microorganism was grown on the hydrolyzed sago starch with an initial sugar concentration of 30 g/lIt was found that the best value of the maximum specific growth rate (µmax) was  0,188 h-1. Kinetic parameters at 96 hour-batch fermentation showed the respective final cell and PHA concentrations of 4,41 g/L and 1,44 g/L. The yields of cell biomass (Yx/s), PHAs per gram of cells (Yp/x), PHAs per gram of substrate (Yp/s) and the percentage of substrate consumption  (∆S/So) were, 0.15 g cell/g sugar; 0.35 g PHA/g cells; 0.06 g PHA/g sugar and 99%, respectively.  The PHAs produced were predominantly composed of poly(3-hydroxybutyrate) (PHB) as the functional groups with a melting point of 163,96 oC.Keywords : hydrolyzed sago starch, PHA, Ralstonia eutropha
Co-Authors . Gunawan . Suharyanto A. Aziz Darwis A. Herryandie Abdul Aziz Darwis ABDULLAH, M Irfani ABDULLAH, M Irfani Aida Farida Anas M. Fauzi Anas M. Fauzi Anas Miftah Fauzi Ani Suryani Anwar Nur Arkenan, Yandra Asep Riswoko ASTUTIATI NURHASANAH Bambang Prasetya Dede Rais Diana Dewi, Diana DIANA NURANI Djoko Said Damardjati Djumali Mangunwidjaja Dwi Purnomo Dwi Setyaningsih DYAH NOOR HIDAYATI E. Gumbira Said E. Gumbira-Sa’id E. Mardliyati Endang Gumbira Said Endang Warsiki Eni Dwi Islamiati, Eni Dwi ERWAHYUNI ENDANG PRABANDARI Erwahyuni Prabandari, Erwahyuni Etik Mardliyati, Etik Etty Riani Fitriani Kasim Gamma Irca Pradikta Gumbira-Sa'id, E Gumbira-Sa’id, E. Han Roliadi Hendri Muchtar, Hendri Iftachul Farida Illah Sailah Irawadi Jamaran IS HELIANTI Lanjar Sumarno Liesbetini Haditjaroko Liesbetini Hartoto Lucky Risanto M. ANWAR NUR Misgiyarta, nFN Muhammad Daud Muhammad Tasrif Mulyorini Rahayuningsih Munarso, S Joni N. A. Evalia Nabilah, Siti Nabilah, Siti Nastiti Siswi Indrasti NIKNIK NURHAYATI Nur Atifah Nur Richana Nyoman J Wistara Nyoman Jaya Wistara, Nyoman Nyoman WISTARA PERMATASARI, Shinta Prabandani, Erwahyuni E PUJI LESTARI Purbowati, Ike Sitoresmi M PUTRI, FENTRI PARAMITHA Rini Purnawati Rofiq Sunaryanto S Heris Anita, S Heris Sarifah Nurjanah Sri, Herastuti Sudirman Sudirman Suprihatin Suprihatin Suryadarma, Prayoga TRI-PANJI, . TRI-PANJI, . Tun T. Irawadi, Tun T. Tun Tedja Irawadi UNTUNG MURDIYATMO Wahono Sumaryono Wasrin Safii Wasrin Syafii Widya FATRIASARI Yandra Arkeman Yeni, Gustri Yernisa Yernisa