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Journal : Jurnal AgroBiogen

Isolasi Identifikasi Bakteri Penghasil Xilanase serta Karakterisasi Enzimnya Richana, Nur; Irawadi, Tun T.; Nur, Anwar; Syamsu, Khaswar
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Abstract

Xylanase is an extracellular enzyme produced bymicroorganisms. This enzyme is able to hydrolise xylane(hemicellulose) to produce xylooligosaccharide and xylose.Thermoalkaliphilic xylanase is an agent that can be used asa substitute in the pulp whitening process instead of chlorine.A study was done to isolate, identificate of bacteria andcharacterize xylanase. The isolation of xylanase producingbacteria has been done from soil and waste of starch industry.Colonies which produced clearing zone were presumedas xylanolytic bacteria and chosen for further screening.Identification of potential isolate in xylanase production wasdone using 16S ribosomal RNA sequencing. Isolate Bacilluspumilus RXA-III5 originated from lime or alkaline soil wasmore potential isolate in xylanase production than other 24isolates. Precipitation of xylanase, that was done usingammonium sulphate followed by dialyzes produced xylanaseof a higher specific activity (267.1 U.mg-1) than that usingacetone (131.1 U.mg-1) and ethanol (186.65 U.mg-1). Xylanasewas done at purification produced three fractions of xylanase.Xylanase characteristics consist of pH and temperature(9 and 50oC), Km and Vmaks value 6 mg.ml-1 and 0.2mol.minute-1, respectively. The Fe2+ was the strongest activetorand Mg2+ was the strongest inhibitor activity. This enzymewas detected as a cellulose-free xylanase. Xylanase is aprospective agent for bio-bleaching of paper.
Purifikasi dan Karakterisasi α-amilase Termostabil dari Bacillus stearothermophilus TII-12 Lestari, Puji; Richana, Nur; Darwis, Abdul Aziz; Syamsu, Khaswar; Murdiyatmo, Untung
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Abstract

Purification and Characterization of Thermostableα-amylase from Bacillus stearothermophilus TII-12. PujiLestari, Nur Richana, Abdul A. Darwis, Khaswar Syamsu,and Untung Murdiyatmo. Thermostable α-amylase is apotential enzyme employed in the starch processing andwidely used in food industries, but this enzyme is stillimported. The local enzyme production would be moreeconomist and useful for its broad applications. Here wereport α-amylase from indigenous bacteria TII-12 which waspurified and characterized, as well as analyzed its hydrolysisproduct on cassava starch. The enzyme of Bacillusstearothermophilus TII-12 partially purified by ultrafiltration,acetone precipitation and gel filtration (Sephadex G-100)showed the reduced total activity, total protein and yield, butincreased the specific activity. The enzyme had a Km of 1,06mg/ml and Vmax of 1,21 mol/min, with optimal activity at pH 7and 90oC. An apparent molecular mass was of 192.932,8Dalton, as estimated by Native-Polyacrylamide Agarose Gelelectrophoresis. Its activity was inhibited by the divalentcation chelator such as EDTA and CuSO4 but activated bycalcium ion. Hydrolysis products of this enzyme on cassavastarch were glucose, dextrin, maltose and oligosaccharides.After 24 hours of hydrolysis, the concentration of glucoseand maltose reached 51.970 and 10.090 ppm, respectively.The thermostable α-amylase of TII-12 is an endo-α-amylaseand prospective to be applied on starch liquefaction withhigh temperature process.
Co-Authors . Gunawan . Suharyanto A. Aziz Darwis A. Herryandie Abdul Aziz Darwis ABDULLAH, M Irfani ABDULLAH, M Irfani Aida Farida Anas M. Fauzi Anas M. Fauzi Anas Miftah Fauzi Ani Suryani Anwar Nur Arkenan, Yandra Asep Riswoko ASTUTIATI NURHASANAH Bambang Prasetya Dede Rais Diana Dewi, Diana DIANA NURANI Djoko Said Damardjati Djumali Mangunwidjaja Dwi Purnomo Dwi Setyaningsih DYAH NOOR HIDAYATI E. Gumbira Said E. Gumbira-Sa’id E. Mardliyati Endang Gumbira Said Endang Warsiki Eni Dwi Islamiati, Eni Dwi ERWAHYUNI ENDANG PRABANDARI Erwahyuni Prabandari, Erwahyuni Etik Mardliyati, Etik Etty Riani Fitriani Kasim Gamma Irca Pradikta Gumbira-Sa'id, E Gumbira-Sa’id, E. Han Roliadi Hendri Muchtar, Hendri Iftachul Farida Illah Sailah Irawadi Jamaran IS HELIANTI Lanjar Sumarno Liesbetini Haditjaroko Liesbetini Hartoto Lucky Risanto M. ANWAR NUR Misgiyarta, nFN Muhammad Daud Muhammad Tasrif Mulyorini Rahayuningsih Munarso, S Joni N. A. Evalia Nabilah, Siti Nabilah, Siti Nastiti Siswi Indrasti NIKNIK NURHAYATI Nur Atifah Nur Richana Nyoman J Wistara Nyoman Jaya Wistara, Nyoman Nyoman WISTARA PERMATASARI, Shinta Prabandani, Erwahyuni E PUJI LESTARI Purbowati, Ike Sitoresmi M PUTRI, FENTRI PARAMITHA Rini Purnawati Rofiq Sunaryanto S Heris Anita, S Heris Sarifah Nurjanah Sri, Herastuti Sudirman Sudirman Suprihatin Suprihatin Suryadarma, Prayoga TRI-PANJI, . TRI-PANJI, . Tun T. Irawadi, Tun T. Tun Tedja Irawadi UNTUNG MURDIYATMO Wahono Sumaryono Wasrin Safii Wasrin Syafii Widya FATRIASARI Yandra Arkeman Yeni, Gustri Yernisa Yernisa